Restriction map for
pF1KB6346
(Linear) (Six-base) MAPSORT of: KIBB6346.seq Check: 1026 from: 1 to: 873
KIBB6346.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 0 803 873
Size: 803 70
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 873
Size: 7 866
AlwNI CAG_nnn'CTG
Cuts at: 0 30 873
Size: 30 843
ApaI G_GGCC'C
Cuts at: 0 659 873
Size: 659 214
ApoI r'AATT_y
Cuts at: 0 595 873
Size: 595 278
BamHI G'GATC_C
Cuts at: 0 178 873
Size: 178 695
BanII G_rGCy'C
Cuts at: 0 659 746 873
Size: 659 87 127
Fragments arranged by size:
659 127 87
BbvCI CC'TCA_GC
Cuts at: 0 142 873
Size: 142 731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 745 779 873
Size: 745 34 94
Fragments arranged by size:
745 94 34
Bme1580I G_kGCm'C
Cuts at: 0 659 768 873
Size: 659 109 105
Fragments arranged by size:
659 109 105
BmgBI CAC'GTC
Cuts at: 0 578 873
Size: 578 295
BmrI ACTGGGnnnn_n'
Cuts at: 0 480 873
Size: 480 393
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 346 415 571 726 873
Size: 345 1 69 156 155 147
Fragments arranged by size:
345 156 155 147 69 1
Bpu10I CC'TnA_GC
Cuts at: 0 142 409 873
Size: 142 267 464
Fragments arranged by size:
464 267 142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 873
Size: 291 582
BsaAI yAC'GTr
Cuts at: 0 378 873
Size: 378 495
BsaHI Gr'CG_yC
Cuts at: 0 530 800 873
Size: 530 270 73
Fragments arranged by size:
530 270 73
BsaWI w'CCGG_w
Cuts at: 0 109 873
Size: 109 764
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 104 134 275 305 873
Size: 104 30 141 30 568
Fragments arranged by size:
568 141 104 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 68 112 217 601 764 873
Size: 55 13 44 105 384 163 109
Fragments arranged by size:
384 163 109 105 55 44 13
BseYI C'CCAG_C
Cuts at: 0 83 191 320 821 873
Size: 83 108 129 501 52
Fragments arranged by size:
501 129 108 83 52
BsiEI CG_ry'CG
Cuts at: 0 786 873
Size: 786 87
BsiHKAI G_wGCw'C
Cuts at: 0 250 277 482 873
Size: 250 27 205 391
Fragments arranged by size:
391 250 205 27
BsmBI CGTCTCn'nnnn_
Cuts at: 0 106 217 682 873
Size: 106 111 465 191
Fragments arranged by size:
465 191 111 106
Bsp1286I G_dGCh'C
Cuts at: 0 250 277 482 659 746 768 873
Size: 250 27 205 177 87 22 105
Fragments arranged by size:
250 205 177 105 87 27 22
BspEI T'CCGG_A
Cuts at: 0 109 873
Size: 109 764
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 407 463 873
Size: 407 56 410
Fragments arranged by size:
410 407 56
BsrDI GCAATG_nn'
Cuts at: 0 294 444 873
Size: 294 150 429
Fragments arranged by size:
429 294 150
BsrFI r'CCGG_y
Cuts at: 0 545 873
Size: 545 328
BssSI C'ACGA_G
Cuts at: 0 481 873
Size: 481 392
BstXI CCAn_nnnn'nTGG
Cuts at: 0 439 873
Size: 439 434
BstYI r'GATC_y
Cuts at: 0 178 873
Size: 178 695
BtgI C'CryG_G
Cuts at: 0 738 873
Size: 738 135
DraIII CAC_nnn'GTG
Cuts at: 0 567 873
Size: 567 306
EaeI y'GGCC_r
Cuts at: 0 735 783 873
Size: 735 48 90
Fragments arranged by size:
735 90 48
EagI C'GGCC_G
Cuts at: 0 783 873
Size: 783 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 345 346 415 571 726 873
Size: 7 338 1 69 156 155 147
Fragments arranged by size:
338 156 155 147 69 7 1
EcoO109I rG'GnC_Cy
Cuts at: 0 213 342 452 616 656 812 873
Size: 213 129 110 164 40 156 61
Fragments arranged by size:
213 164 156 129 110 61 40
HaeII r_GCGC'y
Cuts at: 0 430 873
Size: 430 443
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 104 136 339 371 873
Size: 104 32 203 32 502
Fragments arranged by size:
502 203 104 32 32
HincII GTy'rAC
Cuts at: 0 468 873
Size: 468 405
MslI CAynn'nnrTG
Cuts at: 0 631 873
Size: 631 242
MspA1I CmG'CkG
Cuts at: 0 195 324 491 788 821 873
Size: 195 129 167 297 33 52
Fragments arranged by size:
297 195 167 129 52 33
NaeI GCC'GGC
Cuts at: 0 547 873
Size: 547 326
NgoMIV G'CCGG_C
Cuts at: 0 545 873
Size: 545 328
PasI CC'CwG_GG
Cuts at: 0 135 279 873
Size: 135 144 594
Fragments arranged by size:
594 144 135
PfoI T'CCnGG_A
Cuts at: 0 511 873
Size: 511 362
PmlI CAC'GTG
Cuts at: 0 378 873
Size: 378 495
PpuMI rG'GwC_Cy
Cuts at: 0 342 452 873
Size: 342 110 421
Fragments arranged by size:
421 342 110
PspOMI G'GGCC_C
Cuts at: 0 655 873
Size: 655 218
PstI C_TGCA'G
Cuts at: 0 98 200 873
Size: 98 102 673
Fragments arranged by size:
673 102 98
PvuII CAG'CTG
Cuts at: 0 195 324 491 873
Size: 195 129 167 382
Fragments arranged by size:
382 195 167 129
SexAI A'CCwGG_T
Cuts at: 0 563 873
Size: 563 310
SfcI C'TryA_G
Cuts at: 0 94 196 873
Size: 94 102 677
Fragments arranged by size:
677 102 94
SmlI C'TyrA_G
Cuts at: 0 270 873
Size: 270 603
StuI AGG'CCT
Cuts at: 0 520 873
Size: 520 353
StyI C'CwwG_G
Cuts at: 0 5 873
Size: 5 868
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 462 657 873
Size: 292 170 195 216
Fragments arranged by size:
292 216 195 170
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 275 307 873
Size: 275 32 566
Fragments arranged by size:
566 275 32
Tth111I GACn'n_nGTC
Cuts at: 0 799 873
Size: 799 74
XmnI GAAnn'nnTTC
Cuts at: 0 385 873
Size: 385 488
ZraI GAC'GTC
Cuts at: 0 801 873
Size: 801 72
Enzymes that do cut:
AatII AcuI AlwNI ApaI ApoI BamHI BanII BbvCI
BcgI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspMI BsrDI BsrFI BssSI BstXI BstYI
BtgI DraIII EaeI EagI Eco57MI EcoO109I HaeII Hin4I
HincII MslI MspA1I NaeI NgoMIV PasI PfoI PmlI
PpuMI PspOMI PstI PvuII SexAI SfcI SmlI StuI
StyI TaqII TstI Tth111I XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BbsI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI BsaI BsaBI
BsgI BsiWI BsmI BspHI BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NarI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XcmI XhoI XmaI