(Linear) (Six-base) MAPSORT of: KIBB6337.seq  Check: 459  from: 1  to: 786



KIBB6337.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     786 
   Size:         86     700
 
AleI CACnn'nnGTG

Cuts at:      0     473     786 
   Size:        473     313
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     208     240     786 
   Size:        208      32     546

  Fragments arranged by size: 

                 546     208      32
 
BanII G_rGCy'C

Cuts at:      0     246     786 
   Size:        246     540
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     195     786 
   Size:        195     591
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     698     786 
   Size:        698      88
 
BsaBI GATnn'nnATC

Cuts at:      0     545     786 
   Size:        545     241
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      98     786 
   Size:         98     688
 
BseYI C'CCAG_C

Cuts at:      0     163     321     786 
   Size:        163     158     465

  Fragments arranged by size: 

                 465     163     158
 
Bsp1286I G_dGCh'C

Cuts at:      0     246     786 
   Size:        246     540
 
BsrDI GCAATG_nn'

Cuts at:      0     543     630     786 
   Size:        543      87     156

  Fragments arranged by size: 

                 543     156      87
 
BssSI C'ACGA_G

Cuts at:      0     618     710     786 
   Size:        618      92      76

  Fragments arranged by size: 

                 618      92      76
 
BstEII G'GTnAC_C

Cuts at:      0     337     786 
   Size:        337     449
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     529     786 
   Size:        529     257
 
EarI CTCTTCn'nnn_

Cuts at:      0     257     606     786 
   Size:        257     349     180

  Fragments arranged by size: 

                 349     257     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      86     786 
   Size:         86     700
 
EcoO109I rG'GnC_Cy

Cuts at:      0     224     786 
   Size:        224     562
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     358     786 
   Size:        358     428
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     186     218     786 
   Size:        186      32     568

  Fragments arranged by size: 

                 568     186      32
 
HincII GTy'rAC

Cuts at:      0     194     786 
   Size:        194     592
 
MslI CAynn'nnrTG

Cuts at:      0     473     786 
   Size:        473     313
 
MspA1I CmG'CkG

Cuts at:      0     357     786 
   Size:        357     429
 
PpuMI rG'GwC_Cy

Cuts at:      0     224     786 
   Size:        224     562
 
PstI C_TGCA'G

Cuts at:      0     201     786 
   Size:        201     585
 
PvuII CAG'CTG

Cuts at:      0     357     786 
   Size:        357     429
 
SapI GCTCTTCn'nnn_

Cuts at:      0     257     786 
   Size:        257     529
 
SfcI C'TryA_G

Cuts at:      0      12     197     786 
   Size:         12     185     589

  Fragments arranged by size: 

                 589     185      12
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     177     786 
   Size:        177     609
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     235     786 
   Size:        235     551
 

 Enzymes that do cut:

     AcuI     AleI     AloI    BanII     BmrI     BsaI    BsaBI    BseRI
    BseYI Bsp1286I    BsrDI    BssSI   BstEII     DrdI     EarI  Eco57MI
 EcoO109I     FalI    Hin4I   HincII     MslI   MspA1I    PpuMI     PstI
    PvuII     SapI     SfcI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI