Restriction map for
pF1KB6318
(Linear) (Six-base) MAPSORT of: KIBB6318.seq Check: 407 from: 1 to: 843
KIBB6318.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 463 843
Size: 278 185 380
Fragments arranged by size:
380 278 185
AlwNI CAG_nnn'CTG
Cuts at: 0 736 843
Size: 736 107
ApoI r'AATT_y
Cuts at: 0 437 513 843
Size: 437 76 330
Fragments arranged by size:
437 330 76
AseI AT'TA_AT
Cuts at: 0 269 580 830 843
Size: 269 311 250 13
Fragments arranged by size:
311 269 250 13
AvaI C'yCGr_G
Cuts at: 0 95 178 843
Size: 95 83 665
Fragments arranged by size:
665 95 83
BanII G_rGCy'C
Cuts at: 0 53 212 843
Size: 53 159 631
Fragments arranged by size:
631 159 53
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 843
Size: 107 736
BciVI GTATCCnnnnn_n'
Cuts at: 0 395 818 843
Size: 395 423 25
Fragments arranged by size:
423 395 25
BmgBI CAC'GTC
Cuts at: 0 759 843
Size: 759 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 843
Size: 622 221
BsaI GGTCTCn'nnnn_
Cuts at: 0 87 843
Size: 87 756
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 211 843
Size: 211 632
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 843
Size: 102 741
BsiEI CG_ry'CG
Cuts at: 0 162 843
Size: 162 681
BsiHKAI G_wGCw'C
Cuts at: 0 53 843
Size: 53 790
Bsp1286I G_dGCh'C
Cuts at: 0 53 212 843
Size: 53 159 631
Fragments arranged by size:
631 159 53
BsrBI CCG'CTC
Cuts at: 0 214 843
Size: 214 629
BsrDI GCAATG_nn'
Cuts at: 0 811 843
Size: 811 32
BsrGI T'GTAC_A
Cuts at: 0 462 843
Size: 462 381
BstYI r'GATC_y
Cuts at: 0 395 843
Size: 395 448
BtgI C'CryG_G
Cuts at: 0 37 843
Size: 37 806
BtsI GCAGTG_nn'
Cuts at: 0 235 843
Size: 235 608
EaeI y'GGCC_r
Cuts at: 0 159 817 843
Size: 159 658 26
Fragments arranged by size:
658 159 26
EagI C'GGCC_G
Cuts at: 0 159 843
Size: 159 684
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 75 843
Size: 75 768
EcoICRI GAG'CTC
Cuts at: 0 51 843
Size: 51 792
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 463 622 843
Size: 278 185 159 221
Fragments arranged by size:
278 221 185 159
FspI TGC'GCA
Cuts at: 0 227 843
Size: 227 616
HindIII A'AGCT_T
Cuts at: 0 290 843
Size: 290 553
MspA1I CmG'CkG
Cuts at: 0 247 843
Size: 247 596
NcoI C'CATG_G
Cuts at: 0 37 843
Size: 37 806
NdeI CA'TA_TG
Cuts at: 0 506 843
Size: 506 337
NotI GC'GGCC_GC
Cuts at: 0 159 843
Size: 159 684
PflMI CCAn_nnn'nTGG
Cuts at: 0 78 843
Size: 78 765
PfoI T'CCnGG_A
Cuts at: 0 398 742 843
Size: 398 344 101
Fragments arranged by size:
398 344 101
PsiI TTA'TAA
Cuts at: 0 706 843
Size: 706 137
PvuII CAG'CTG
Cuts at: 0 247 843
Size: 247 596
SacI G_AGCT'C
Cuts at: 0 53 843
Size: 53 790
ScaI AGT'ACT
Cuts at: 0 329 843
Size: 329 514
StuI AGG'CCT
Cuts at: 0 76 843
Size: 76 767
StyI C'CwwG_G
Cuts at: 0 37 77 843
Size: 37 40 766
Fragments arranged by size:
766 40 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 154 843
Size: 154 689
TatI w'GTAC_w
Cuts at: 0 327 462 843
Size: 327 135 381
Fragments arranged by size:
381 327 135
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 843
Size: 396 447
XmnI GAAnn'nnTTC
Cuts at: 0 104 843
Size: 104 739
Enzymes that do cut:
AcuI AlwNI ApoI AseI AvaI BanII BbsI BciVI
BmgBI BpmI BsaI BseRI BsgI BsiEI BsiHKAI Bsp1286I
BsrBI BsrDI BsrGI BstYI BtgI BtsI EaeI EagI
EciI EcoICRI Eco57MI FspI HindIII MspA1I NcoI NdeI
NotI PflMI PfoI PsiI PvuII SacI ScaI StuI
StyI TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BclI BfrBI BglI BglII BlpI Bme1580I BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoNI
EcoO109I EcoRI EcoRV FalI FseI FspAI HaeII Hin4I
HincII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NgoMIV NheI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmaI ZraI