Restriction map for
pF1KB6310
(Linear) (Six-base) MAPSORT of: KIBB6310.seq Check: 722 from: 1 to: 840
KIBB6310.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 607 840
Size: 565 42 233
Fragments arranged by size:
565 233 42
AfeI AGC'GCT
Cuts at: 0 492 840
Size: 492 348
AhdI GACnn_n'nnGTC
Cuts at: 0 213 840
Size: 213 627
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 814 840
Size: 814 26
ApaI G_GGCC'C
Cuts at: 0 750 840
Size: 750 90
AvaI C'yCGr_G
Cuts at: 0 93 317 526 742 757 777 840
Size: 93 224 209 216 15 20 63
Fragments arranged by size:
224 216 209 93 63 20 15
BamHI G'GATC_C
Cuts at: 0 162 840
Size: 162 678
BanII G_rGCy'C
Cuts at: 0 7 680 750 784 840
Size: 7 673 70 34 56
Fragments arranged by size:
673 70 56 34 7
BbvCI CC'TCA_GC
Cuts at: 0 392 840
Size: 392 448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 448 482 840
Size: 448 34 358
Fragments arranged by size:
448 358 34
BglI GCCn_nnn'nGGC
Cuts at: 0 367 542 840
Size: 367 175 298
Fragments arranged by size:
367 298 175
BglII A'GATC_T
Cuts at: 0 84 561 840
Size: 84 477 279
Fragments arranged by size:
477 279 84
Bme1580I G_kGCm'C
Cuts at: 0 39 363 750 840
Size: 39 324 387 90
Fragments arranged by size:
387 324 90 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 590 840
Size: 590 250
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 195 375 586 840
Size: 101 94 180 211 254
Fragments arranged by size:
254 211 180 101 94
Bpu10I CC'TnA_GC
Cuts at: 0 392 840
Size: 392 448
BsaI GGTCTCn'nnnn_
Cuts at: 0 14 840
Size: 14 826
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 99 129 790 820 840
Size: 99 30 661 30 20
Fragments arranged by size:
661 99 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 529 624 688 832 840
Size: 529 95 64 144 8
Fragments arranged by size:
529 144 95 64 8
BseYI C'CCAG_C
Cuts at: 0 362 374 387 640 682 709 840
Size: 362 12 13 253 42 27 131
Fragments arranged by size:
362 253 131 42 27 13 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 505 840
Size: 295 210 335
Fragments arranged by size:
335 295 210
Bsp1286I G_dGCh'C
Cuts at: 0 7 39 363 680 750 784 840
Size: 7 32 324 317 70 34 56
Fragments arranged by size:
324 317 70 56 34 32 7
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 840
Size: 312 528
BstYI r'GATC_y
Cuts at: 0 84 162 561 840
Size: 84 78 399 279
Fragments arranged by size:
399 279 84 78
Bsu36I CC'TnA_GG
Cuts at: 0 671 840
Size: 671 169
BtgI C'CryG_G
Cuts at: 0 110 305 311 794 840
Size: 110 195 6 483 46
Fragments arranged by size:
483 195 110 46 6
EaeI y'GGCC_r
Cuts at: 0 248 308 840
Size: 248 60 532
Fragments arranged by size:
532 248 60
EarI CTCTTCn'nnn_
Cuts at: 0 506 840
Size: 506 334
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 195 375 565 586 607 840
Size: 101 94 180 190 21 21 233
Fragments arranged by size:
233 190 180 101 94 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 814 840
Size: 814 26
EcoO109I rG'GnC_Cy
Cuts at: 0 215 746 754 767 840
Size: 215 531 8 13 73
Fragments arranged by size:
531 215 73 13 8
HaeII r_GCGC'y
Cuts at: 0 494 840
Size: 494 346
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 199 231 790 822 840
Size: 76 32 91 32 559 32 18
Fragments arranged by size:
559 91 76 32 32 32 18
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 230 840
Size: 230 610
MscI TGG'CCA
Cuts at: 0 250 840
Size: 250 590
MslI CAynn'nnrTG
Cuts at: 0 310 840
Size: 310 530
MspA1I CmG'CkG
Cuts at: 0 366 391 659 691 803 840
Size: 366 25 268 32 112 37
Fragments arranged by size:
366 268 112 37 32 25
NcoI C'CATG_G
Cuts at: 0 311 794 840
Size: 311 483 46
Fragments arranged by size:
483 311 46
NruI TCG'CGA
Cuts at: 0 18 840
Size: 18 822
PasI CC'CwG_GG
Cuts at: 0 60 540 840
Size: 60 480 300
Fragments arranged by size:
480 300 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 564 840
Size: 564 276
PfoI T'CCnGG_A
Cuts at: 0 416 840
Size: 416 424
PpuMI rG'GwC_Cy
Cuts at: 0 215 754 767 840
Size: 215 539 13 73
Fragments arranged by size:
539 215 73 13
PshAI GACnn'nnGTC
Cuts at: 0 659 840
Size: 659 181
PspOMI G'GGCC_C
Cuts at: 0 746 840
Size: 746 94
PstI C_TGCA'G
Cuts at: 0 450 725 840
Size: 450 275 115
Fragments arranged by size:
450 275 115
PvuII CAG'CTG
Cuts at: 0 391 659 840
Size: 391 268 181
Fragments arranged by size:
391 268 181
SanDI GG'GwC_CC
Cuts at: 0 767 840
Size: 767 73
SfcI C'TryA_G
Cuts at: 0 446 721 840
Size: 446 275 119
Fragments arranged by size:
446 275 119
SmaI CCC'GGG
Cuts at: 0 744 779 840
Size: 744 35 61
Fragments arranged by size:
744 61 35
StuI AGG'CCT
Cuts at: 0 628 840
Size: 628 212
StyI C'CwwG_G
Cuts at: 0 166 311 750 794 840
Size: 166 145 439 44 46
Fragments arranged by size:
439 166 145 46 44
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 99 131 840
Size: 99 32 709
Fragments arranged by size:
709 99 32
Tth111I GACn'n_nGTC
Cuts at: 0 124 840
Size: 124 716
XmaI C'CCGG_G
Cuts at: 0 742 777 840
Size: 742 35 63
Fragments arranged by size:
742 63 35
Enzymes that do cut:
AcuI AfeI AhdI AloI ApaI AvaI BamHI BanII
BbvCI BcgI BglI BglII Bme1580I BmrI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsgI Bsp1286I BstXI BstYI
Bsu36I BtgI EaeI EarI Eco57MI EcoNI EcoO109I HaeII
Hin4I MmeI MscI MslI MspA1I NcoI NruI PasI
PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SanDI
SfcI SmaI StuI StyI TstI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BanI BbeI BbsI BciVI BclI BfrBI
BlpI BmgBI BmtI BplI BpuEI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NsiI NspI PacI PciI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
XbaI XcmI XhoI XmnI ZraI