Restriction map for
pF1KB6305
(Linear) (Six-base) MAPSORT of: KIBB6305.seq Check: 5639 from: 1 to: 774
KIBB6305.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AgeI A'CCGG_T
Cuts at: 0 113 774
Size: 113 661
AleI CACnn'nnGTG
Cuts at: 0 140 568 774
Size: 140 428 206
Fragments arranged by size:
428 206 140
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 607 774
Size: 575 32 167
Fragments arranged by size:
575 167 32
AlwNI CAG_nnn'CTG
Cuts at: 0 431 486 774
Size: 431 55 288
Fragments arranged by size:
431 288 55
ApaI G_GGCC'C
Cuts at: 0 625 774
Size: 625 149
ApoI r'AATT_y
Cuts at: 0 81 774
Size: 81 693
BanII G_rGCy'C
Cuts at: 0 550 625 774
Size: 550 75 149
Fragments arranged by size:
550 149 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 211 711 774
Size: 211 500 63
Fragments arranged by size:
500 211 63
BglI GCCn_nnn'nGGC
Cuts at: 0 415 774
Size: 415 359
Bme1580I G_kGCm'C
Cuts at: 0 625 774
Size: 625 149
BmrI ACTGGGnnnn_n'
Cuts at: 0 177 552 774
Size: 177 375 222
Fragments arranged by size:
375 222 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 140 245 513 687 774
Size: 91 49 105 268 174 87
Fragments arranged by size:
268 174 105 91 87 49
Bpu10I CC'TnA_GC
Cuts at: 0 238 774
Size: 238 536
BsaI GGTCTCn'nnnn_
Cuts at: 0 221 735 774
Size: 221 514 39
Fragments arranged by size:
514 221 39
BsaAI yAC'GTr
Cuts at: 0 267 774
Size: 267 507
BsaWI w'CCGG_w
Cuts at: 0 36 113 774
Size: 36 77 661
Fragments arranged by size:
661 77 36
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 532 562 575 605 774
Size: 532 30 13 30 169
Fragments arranged by size:
532 169 30 30 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 525 774
Size: 525 249
BseYI C'CCAG_C
Cuts at: 0 384 446 774
Size: 384 62 328
Fragments arranged by size:
384 328 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 774
Size: 295 479
Bsp1286I G_dGCh'C
Cuts at: 0 550 625 774
Size: 550 75 149
Fragments arranged by size:
550 149 75
BspEI T'CCGG_A
Cuts at: 0 36 774
Size: 36 738
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 509 774
Size: 509 265
BsrDI GCAATG_nn'
Cuts at: 0 630 774
Size: 630 144
BsrFI r'CCGG_y
Cuts at: 0 113 322 774
Size: 113 209 452
Fragments arranged by size:
452 209 113
BstXI CCAn_nnnn'nTGG
Cuts at: 0 250 774
Size: 250 524
BtgI C'CryG_G
Cuts at: 0 566 660 774
Size: 566 94 114
Fragments arranged by size:
566 114 94
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 318 747 774
Size: 318 429 27
Fragments arranged by size:
429 318 27
BtsI GCAGTG_nn'
Cuts at: 0 186 774
Size: 186 588
DraIII CAC_nnn'GTG
Cuts at: 0 192 774
Size: 192 582
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 140 245 513 687 774
Size: 91 49 105 268 174 87
Fragments arranged by size:
268 174 105 91 87 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 490 774
Size: 490 284
EcoO109I rG'GnC_Cy
Cuts at: 0 30 149 621 622 774
Size: 30 119 472 1 152
Fragments arranged by size:
472 152 119 30 1
EcoRI G'AATT_C
Cuts at: 0 81 774
Size: 81 693
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 168 200 774
Size: 168 32 574
Fragments arranged by size:
574 168 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 774
Size: 39 735
MslI CAynn'nnrTG
Cuts at: 0 140 568 774
Size: 140 428 206
Fragments arranged by size:
428 206 140
MspA1I CmG'CkG
Cuts at: 0 181 384 474 774
Size: 181 203 90 300
Fragments arranged by size:
300 203 181 90
NaeI GCC'GGC
Cuts at: 0 324 774
Size: 324 450
NcoI C'CATG_G
Cuts at: 0 566 660 774
Size: 566 94 114
Fragments arranged by size:
566 114 94
NgoMIV G'CCGG_C
Cuts at: 0 322 774
Size: 322 452
PflMI CCAn_nnn'nTGG
Cuts at: 0 636 774
Size: 636 138
PfoI T'CCnGG_A
Cuts at: 0 703 745 774
Size: 703 42 29
Fragments arranged by size:
703 42 29
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 575 607 774
Size: 575 32 167
Fragments arranged by size:
575 167 32
PpuMI rG'GwC_Cy
Cuts at: 0 30 149 774
Size: 30 119 625
Fragments arranged by size:
625 119 30
PspOMI G'GGCC_C
Cuts at: 0 621 774
Size: 621 153
PstI C_TGCA'G
Cuts at: 0 491 774
Size: 491 283
PvuII CAG'CTG
Cuts at: 0 181 384 474 774
Size: 181 203 90 300
Fragments arranged by size:
300 203 181 90
SanDI GG'GwC_CC
Cuts at: 0 149 774
Size: 149 625
SfcI C'TryA_G
Cuts at: 0 487 713 774
Size: 487 226 61
Fragments arranged by size:
487 226 61
StyI C'CwwG_G
Cuts at: 0 566 660 774
Size: 566 94 114
Fragments arranged by size:
566 114 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 159 376 739 774
Size: 40 119 217 363 35
Fragments arranged by size:
363 217 119 40 35
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 115 471 613 774
Size: 115 356 142 161
Fragments arranged by size:
356 161 142 115
XmnI GAAnn'nnTTC
Cuts at: 0 208 774
Size: 208 566
Enzymes that do cut:
AgeI AleI AloI AlwNI ApaI ApoI BanII BbsI
BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrDI
BsrFI BstXI BtgI BtgZI BtsI DraIII Eco57MI EcoNI
EcoO109I EcoRI Hin4I MmeI MslI MspA1I NaeI NcoI
NgoMIV PflMI PfoI PpiI PpuMI PspOMI PstI PvuII
SanDI SfcI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AhdI AlfI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI
BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XhoI XmaI ZraI