Restriction map for
pF1KB6301
(Linear) (Six-base) MAPSORT of: KIBB6301.seq Check: 2156 from: 1 to: 750
KIBB6301.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 228 750
Size: 228 522
AccI GT'mk_AC
Cuts at: 0 233 750
Size: 233 517
ApoI r'AATT_y
Cuts at: 0 704 750
Size: 704 46
AvaI C'yCGr_G
Cuts at: 0 136 750
Size: 136 614
BanI G'GyrC_C
Cuts at: 0 144 171 181 322 378 750
Size: 144 27 10 141 56 372
Fragments arranged by size:
372 144 141 56 27 10
BbeI G_GCGC'C
Cuts at: 0 185 326 750
Size: 185 141 424
Fragments arranged by size:
424 185 141
BglI GCCn_nnn'nGGC
Cuts at: 0 320 750
Size: 320 430
Bme1580I G_kGCm'C
Cuts at: 0 409 750
Size: 409 341
BmrI ACTGGGnnnn_n'
Cuts at: 0 549 709 750
Size: 549 160 41
Fragments arranged by size:
549 160 41
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 212 221 248 750
Size: 161 51 9 27 502
Fragments arranged by size:
502 161 51 27 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 41 750
Size: 41 709
BsaHI Gr'CG_yC
Cuts at: 0 182 323 750
Size: 182 141 427
Fragments arranged by size:
427 182 141
BsaWI w'CCGG_w
Cuts at: 0 292 750
Size: 292 458
BseYI C'CCAG_C
Cuts at: 0 91 247 331 750
Size: 91 156 84 419
Fragments arranged by size:
419 156 91 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 750
Size: 206 544
BsiHKAI G_wGCw'C
Cuts at: 0 198 219 750
Size: 198 21 531
Fragments arranged by size:
531 198 21
Bsp1286I G_dGCh'C
Cuts at: 0 198 219 409 750
Size: 198 21 190 341
Fragments arranged by size:
341 198 190 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 228 365 750
Size: 228 137 385
Fragments arranged by size:
385 228 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 91 750
Size: 91 659
BtsI GCAGTG_nn'
Cuts at: 0 641 750
Size: 641 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 212 221 248 750
Size: 161 51 9 27 502
Fragments arranged by size:
502 161 51 27 9
EcoO109I rG'GnC_Cy
Cuts at: 0 128 260 750
Size: 128 132 490
Fragments arranged by size:
490 132 128
HaeII r_GCGC'y
Cuts at: 0 185 326 750
Size: 185 141 424
Fragments arranged by size:
424 185 141
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 750
Size: 16 32 702
Fragments arranged by size:
702 32 16
KasI G'GCGC_C
Cuts at: 0 181 322 750
Size: 181 141 428
Fragments arranged by size:
428 181 141
MspA1I CmG'CkG
Cuts at: 0 251 750
Size: 251 499
NarI GG'CG_CC
Cuts at: 0 182 323 750
Size: 182 141 427
Fragments arranged by size:
427 182 141
NspI r_CATG'y
Cuts at: 0 390 750
Size: 390 360
PasI CC'CwG_GG
Cuts at: 0 109 151 238 256 750
Size: 109 42 87 18 494
Fragments arranged by size:
494 109 87 42 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 274 750
Size: 274 476
PfoI T'CCnGG_A
Cuts at: 0 162 210 750
Size: 162 48 540
Fragments arranged by size:
540 162 48
PsiI TTA'TAA
Cuts at: 0 353 750
Size: 353 397
ScaI AGT'ACT
Cuts at: 0 605 750
Size: 605 145
SfoI GGC'GCC
Cuts at: 0 183 324 750
Size: 183 141 426
Fragments arranged by size:
426 183 141
SmaI CCC'GGG
Cuts at: 0 138 750
Size: 138 612
SmlI C'TyrA_G
Cuts at: 0 56 750
Size: 56 694
SphI G_CATG'C
Cuts at: 0 390 750
Size: 390 360
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 261 750
Size: 261 489
TatI w'GTAC_w
Cuts at: 0 603 659 750
Size: 603 56 91
Fragments arranged by size:
603 91 56
XmaI C'CCGG_G
Cuts at: 0 136 750
Size: 136 614
XmnI GAAnn'nnTTC
Cuts at: 0 589 750
Size: 589 161
Enzymes that do cut:
AarI AccI ApoI AvaI BanI BbeI BglI Bme1580I
BmrI BpmI BpuEI BsaHI BsaWI BseYI BsgI BsiHKAI
Bsp1286I BspMI BstXI BtsI Eco57MI EcoO109I HaeII Hin4I
KasI MspA1I NarI NspI PasI PflMI PfoI PsiI
ScaI SfoI SmaI SmlI SphI TaqII TatI XmaI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaXI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfcI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI StuI StyI SwaI TstI Tth111I XbaI
XcmI XhoI ZraI