Restriction map for
pF1KB6253
(Linear) (Six-base) MAPSORT of: KIBB6253.seq Check: 8851 from: 1 to: 1920
KIBB6253.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 927 1920
Size: 927 993
AclI AA'CG_TT
Cuts at: 0 339 1920
Size: 339 1581
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 770 1115 1471 1588 1920
Size: 172 598 345 356 117 332
Fragments arranged by size:
598 356 345 332 172 117
AflIII A'CryG_T
Cuts at: 0 795 1920
Size: 795 1125
AlwNI CAG_nnn'CTG
Cuts at: 0 1820 1920
Size: 1820 100
ApaLI G'TGCA_C
Cuts at: 0 1025 1920
Size: 1025 895
ApoI r'AATT_y
Cuts at: 0 555 1171 1920
Size: 555 616 749
Fragments arranged by size:
749 616 555
AvaI C'yCGr_G
Cuts at: 0 131 973 1920
Size: 131 842 947
Fragments arranged by size:
947 842 131
BamHI G'GATC_C
Cuts at: 0 1077 1920
Size: 1077 843
BanI G'GyrC_C
Cuts at: 0 1138 1725 1920
Size: 1138 587 195
Fragments arranged by size:
1138 587 195
BanII G_rGCy'C
Cuts at: 0 269 1798 1920
Size: 269 1529 122
Fragments arranged by size:
1529 269 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 15 458 596 866 1751 1920
Size: 15 443 138 270 885 169
Fragments arranged by size:
885 443 270 169 138 15
BbvCI CC'TCA_GC
Cuts at: 0 818 1920
Size: 818 1102
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1782 1816 1920
Size: 1782 34 104
Fragments arranged by size:
1782 104 34
BclI T'GATC_A
Cuts at: 0 1502 1920
Size: 1502 418
BfrBI ATG'CAT
Cuts at: 0 1894 1920
Size: 1894 26
BglII A'GATC_T
Cuts at: 0 1488 1920
Size: 1488 432
Bme1580I G_kGCm'C
Cuts at: 0 1029 1141 1920
Size: 1029 112 779
Fragments arranged by size:
1029 779 112
BmrI ACTGGGnnnn_n'
Cuts at: 0 493 1644 1920
Size: 493 1151 276
Fragments arranged by size:
1151 493 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 750 1920
Size: 750 1170
Bpu10I CC'TnA_GC
Cuts at: 0 263 671 818 1920
Size: 263 408 147 1102
Fragments arranged by size:
1102 408 263 147
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1412 1920
Size: 1412 508
BsaI GGTCTCn'nnnn_
Cuts at: 0 1304 1920
Size: 1304 616
BsaAI yAC'GTr
Cuts at: 0 1887 1920
Size: 1887 33
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1864 1920
Size: 1864 56
BseYI C'CCAG_C
Cuts at: 0 1356 1920
Size: 1356 564
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 1920
Size: 247 1673
BsiHKAI G_wGCw'C
Cuts at: 0 269 1029 1920
Size: 269 760 891
Fragments arranged by size:
891 760 269
BsmI GAATG_Cn'
Cuts at: 0 1894 1896 1920
Size: 1894 2 24
Fragments arranged by size:
1894 24 2
Bsp1286I G_dGCh'C
Cuts at: 0 269 1029 1141 1798 1920
Size: 269 760 112 657 122
Fragments arranged by size:
760 657 269 122 112
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 927 1836 1920
Size: 927 909 84
Fragments arranged by size:
927 909 84
BsrDI GCAATG_nn'
Cuts at: 0 992 1920
Size: 992 928
BsrGI T'GTAC_A
Cuts at: 0 318 1920
Size: 318 1602
BstYI r'GATC_y
Cuts at: 0 1077 1488 1920
Size: 1077 411 432
Fragments arranged by size:
1077 432 411
BtsI GCAGTG_nn'
Cuts at: 0 39 235 632 1920
Size: 39 196 397 1288
Fragments arranged by size:
1288 397 196 39
EaeI y'GGCC_r
Cuts at: 0 629 705 1920
Size: 629 76 1215
Fragments arranged by size:
1215 629 76
EarI CTCTTCn'nnn_
Cuts at: 0 131 440 1920
Size: 131 309 1480
Fragments arranged by size:
1480 309 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 668 1920
Size: 668 1252
EcoICRI GAG'CTC
Cuts at: 0 267 1920
Size: 267 1653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 750 770 1115 1471 1588 1920
Size: 172 578 20 345 356 117 332
Fragments arranged by size:
578 356 345 332 172 117 20
EcoO109I rG'GnC_Cy
Cuts at: 0 277 1784 1920
Size: 277 1507 136
Fragments arranged by size:
1507 277 136
EcoRI G'AATT_C
Cuts at: 0 555 1920
Size: 555 1365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 126 1182 1920
Size: 126 1056 738
Fragments arranged by size:
1056 738 126
HaeII r_GCGC'y
Cuts at: 0 1106 1920
Size: 1106 814
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1069 1101 1446 1458 1478 1490 1576 1608
Size: 1069 32 345 12 20 12 86 32
Cuts at: 1608 1920
Size: 312
Fragments arranged by size:
1069 345 312 86 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 720 911 1920
Size: 720 191 1009
Fragments arranged by size:
1009 720 191
HindIII A'AGCT_T
Cuts at: 0 636 1920
Size: 636 1284
HpaI GTT'AAC
Cuts at: 0 720 1920
Size: 720 1200
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 1621 1920
Size: 550 1071 299
Fragments arranged by size:
1071 550 299
MscI TGG'CCA
Cuts at: 0 631 707 1920
Size: 631 76 1213
Fragments arranged by size:
1213 631 76
MslI CAynn'nnrTG
Cuts at: 0 74 851 1920
Size: 74 777 1069
Fragments arranged by size:
1069 777 74
MspA1I CmG'CkG
Cuts at: 0 826 1075 1920
Size: 826 249 845
Fragments arranged by size:
845 826 249
NsiI A_TGCA'T
Cuts at: 0 1896 1920
Size: 1896 24
NspI r_CATG'y
Cuts at: 0 799 965 1417 1920
Size: 799 166 452 503
Fragments arranged by size:
799 503 452 166
PciI A'CATG_T
Cuts at: 0 795 1920
Size: 795 1125
PfoI T'CCnGG_A
Cuts at: 0 436 1920
Size: 436 1484
PpuMI rG'GwC_Cy
Cuts at: 0 277 1784 1920
Size: 277 1507 136
Fragments arranged by size:
1507 277 136
PshAI GACnn'nnGTC
Cuts at: 0 1912 1920
Size: 1912 8
PstI C_TGCA'G
Cuts at: 0 599 1496 1920
Size: 599 897 424
Fragments arranged by size:
897 599 424
PvuII CAG'CTG
Cuts at: 0 826 1920
Size: 826 1094
RsrII CG'GwC_CG
Cuts at: 0 331 1920
Size: 331 1589
SacI G_AGCT'C
Cuts at: 0 269 1920
Size: 269 1651
ScaI AGT'ACT
Cuts at: 0 254 1920
Size: 254 1666
SfcI C'TryA_G
Cuts at: 0 595 683 785 1492 1920
Size: 595 88 102 707 428
Fragments arranged by size:
707 595 428 102 88
SmaI CCC'GGG
Cuts at: 0 133 975 1920
Size: 133 842 945
Fragments arranged by size:
945 842 133
SmlI C'TyrA_G
Cuts at: 0 1427 1920
Size: 1427 493
SspI AAT'ATT
Cuts at: 0 1701 1920
Size: 1701 219
StyI C'CwwG_G
Cuts at: 0 280 1920
Size: 280 1640
TatI w'GTAC_w
Cuts at: 0 252 318 1920
Size: 252 66 1602
Fragments arranged by size:
1602 252 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 923 955 1920
Size: 923 32 965
Fragments arranged by size:
965 923 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 76 1920
Size: 76 1844
XmaI C'CCGG_G
Cuts at: 0 131 973 1920
Size: 131 842 947
Fragments arranged by size:
947 842 131
XmnI GAAnn'nnTTC
Cuts at: 0 1894 1920
Size: 1894 26
Enzymes that do cut:
AarI AclI AcuI AflIII AlwNI ApaLI ApoI AvaI
BamHI BanI BanII BbsI BbvCI BcgI BclI BfrBI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrGI BstYI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI
MmeI MscI MslI MspA1I NsiI NspI PciI PfoI
PpuMI PshAI PstI PvuII RsrII SacI ScaI SfcI
SmaI SmlI SspI StyI TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BbeI BciVI BglI BlpI BmgBI BmtI BplI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoNI EcoRV FseI FspI FspAI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PflMI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI StuI SwaI TaqII Tth111I
XbaI XhoI ZraI