Restriction map for
pF1KB6241
(Linear) (Six-base) MAPSORT of: KIBB6241.seq Check: 8454 from: 1 to: 2253
KIBB6241.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 0 1259 2253
Size: 1259 994
AclI AA'CG_TT
Cuts at: 0 1542 2253
Size: 1542 711
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 549 717 1977 2253
Size: 374 175 168 1260 276
Fragments arranged by size:
1260 374 276 175 168
AflIII A'CryG_T
Cuts at: 0 264 2253
Size: 264 1989
AleI CACnn'nnGTG
Cuts at: 0 1532 2207 2253
Size: 1532 675 46
Fragments arranged by size:
1532 675 46
ApaI G_GGCC'C
Cuts at: 0 105 2253
Size: 105 2148
ApaLI G'TGCA_C
Cuts at: 0 1009 2253
Size: 1009 1244
ApoI r'AATT_y
Cuts at: 0 155 519 2253
Size: 155 364 1734
Fragments arranged by size:
1734 364 155
AvaI C'yCGr_G
Cuts at: 0 1698 2253
Size: 1698 555
BanII G_rGCy'C
Cuts at: 0 91 105 2253
Size: 91 14 2148
Fragments arranged by size:
2148 91 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 231 1780 2253
Size: 231 1549 473
Fragments arranged by size:
1549 473 231
BciVI GTATCCnnnnn_n'
Cuts at: 0 2024 2253
Size: 2024 229
BclI T'GATC_A
Cuts at: 0 327 2253
Size: 327 1926
BglI GCCn_nnn'nGGC
Cuts at: 0 236 2253
Size: 236 2017
BlpI GC'TnA_GC
Cuts at: 0 85 2253
Size: 85 2168
Bme1580I G_kGCm'C
Cuts at: 0 105 209 1013 1527 2253
Size: 105 104 804 514 726
Fragments arranged by size:
804 726 514 105 104
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 110 2253
Size: 110 2143
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 2253
Size: 145 2108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 724 2253
Size: 411 313 1529
Fragments arranged by size:
1529 411 313
BsaAI yAC'GTr
Cuts at: 0 2209 2253
Size: 2209 44
BsaBI GATnn'nnATC
Cuts at: 0 583 2253
Size: 583 1670
BsaHI Gr'CG_yC
Cuts at: 0 228 2253
Size: 228 2025
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 426 456 2223 2253
Size: 426 30 1767 30
Fragments arranged by size:
1767 426 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 447 742 1162 1381 1465 1750 1918 2253
Size: 447 295 420 219 84 285 168 335
Fragments arranged by size:
447 420 335 295 285 219 168 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 566 732 1152 2253
Size: 266 300 166 420 1101
Fragments arranged by size:
1101 420 300 266 166
BsiHKAI G_wGCw'C
Cuts at: 0 1013 2253
Size: 1013 1240
BsmI GAATG_Cn'
Cuts at: 0 535 883 939 2146 2253
Size: 535 348 56 1207 107
Fragments arranged by size:
1207 535 348 107 56
Bsp1286I G_dGCh'C
Cuts at: 0 91 105 209 1013 1527 2253
Size: 91 14 104 804 514 726
Fragments arranged by size:
804 726 514 104 91 14
BsrDI GCAATG_nn'
Cuts at: 0 664 2253
Size: 664 1589
BsrFI r'CCGG_y
Cuts at: 0 230 2253
Size: 230 2023
BsrGI T'GTAC_A
Cuts at: 0 107 2224 2253
Size: 107 2117 29
Fragments arranged by size:
2117 107 29
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1727 2253
Size: 1727 526
BstXI CCAn_nnnn'nTGG
Cuts at: 0 889 1057 2152 2253
Size: 889 168 1095 101
Fragments arranged by size:
1095 889 168 101
Bsu36I CC'TnA_GG
Cuts at: 0 8 1519 2253
Size: 8 1511 734
Fragments arranged by size:
1511 734 8
BtgI C'CryG_G
Cuts at: 0 386 2253
Size: 386 1867
BtsI GCAGTG_nn'
Cuts at: 0 720 1144 1648 2253
Size: 720 424 504 605
Fragments arranged by size:
720 605 504 424
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 786 821 954 989 1374 1409 1878 1913
Size: 786 35 133 35 385 35 469 35
Cuts at: 1913 2253
Size: 340
Fragments arranged by size:
786 469 385 340 133 35 35 35
35
EaeI y'GGCC_r
Cuts at: 0 232 1059 2253
Size: 232 827 1194
Fragments arranged by size:
1194 827 232
EarI CTCTTCn'nnn_
Cuts at: 0 212 1768 1996 2253
Size: 212 1556 228 257
Fragments arranged by size:
1556 257 228 212
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2043 2253
Size: 2043 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 374 549 717 1977 2253
Size: 145 229 175 168 1260 276
Fragments arranged by size:
1260 276 229 175 168 145
EcoO109I rG'GnC_Cy
Cuts at: 0 4 101 102 238 2253
Size: 4 97 1 136 2015
Fragments arranged by size:
2015 136 97 4 1
EcoRI G'AATT_C
Cuts at: 0 155 2253
Size: 155 2098
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1017 1437 2220 2253
Size: 1017 420 783 33
Fragments arranged by size:
1017 783 420 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 465 497 898 930 1391 1423 1486 1518
Size: 465 32 401 32 461 32 63 32
Cuts at: 1518 1727 1759 2233 2253
Size: 209 32 474 20
Fragments arranged by size:
474 465 461 401 209 63 32 32
32 32 32 20
HincII GTy'rAC
Cuts at: 0 1250 1334 1418 2253
Size: 1250 84 84 835
Fragments arranged by size:
1250 835 84 84
HindIII A'AGCT_T
Cuts at: 0 333 1233 2253
Size: 333 900 1020
Fragments arranged by size:
1020 900 333
MslI CAynn'nnrTG
Cuts at: 0 540 1532 2207 2253
Size: 540 992 675 46
Fragments arranged by size:
992 675 540 46
MspA1I CmG'CkG
Cuts at: 0 352 1997 2253
Size: 352 1645 256
Fragments arranged by size:
1645 352 256
NaeI GCC'GGC
Cuts at: 0 232 2253
Size: 232 2021
NcoI C'CATG_G
Cuts at: 0 386 2253
Size: 386 1867
NgoMIV G'CCGG_C
Cuts at: 0 230 2253
Size: 230 2023
NspI r_CATG'y
Cuts at: 0 268 418 629 2253
Size: 268 150 211 1624
Fragments arranged by size:
1624 268 211 150
PasI CC'CwG_GG
Cuts at: 0 209 2253
Size: 209 2044
PciI A'CATG_T
Cuts at: 0 264 2253
Size: 264 1989
PpuMI rG'GwC_Cy
Cuts at: 0 4 2253
Size: 4 2249
PspOMI G'GGCC_C
Cuts at: 0 101 2253
Size: 101 2152
PstI C_TGCA'G
Cuts at: 0 1555 2253
Size: 1555 698
PvuII CAG'CTG
Cuts at: 0 352 1997 2253
Size: 352 1645 256
Fragments arranged by size:
1645 352 256
SfcI C'TryA_G
Cuts at: 0 132 485 1551 1803 2181 2253
Size: 132 353 1066 252 378 72
Fragments arranged by size:
1066 378 353 252 132 72
SmlI C'TyrA_G
Cuts at: 0 426 739 2253
Size: 426 313 1514
Fragments arranged by size:
1514 426 313
StuI AGG'CCT
Cuts at: 0 2133 2253
Size: 2133 120
StyI C'CwwG_G
Cuts at: 0 386 2253
Size: 386 1867
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 1522 2227 2253
Size: 79 1443 705 26
Fragments arranged by size:
1443 705 79 26
TatI w'GTAC_w
Cuts at: 0 107 2224 2253
Size: 107 2117 29
Fragments arranged by size:
2117 107 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 426 458 2223 2253
Size: 426 32 1765 30
Fragments arranged by size:
1765 426 32 30
XmnI GAAnn'nnTTC
Cuts at: 0 1627 1795 1963 2253
Size: 1627 168 168 290
Fragments arranged by size:
1627 290 168 168
Enzymes that do cut:
AccI AclI AcuI AflIII AleI ApaI ApaLI ApoI
AvaI BanII BbsI BciVI BclI BglI BlpI Bme1580I
BplI BpmI BpuEI BsaAI BsaBI BsaHI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BsrGI BstAPI
BstXI Bsu36I BtgI BtsI CspCI EaeI EarI EciI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII HindIII MslI
MspA1I NaeI NcoI NgoMIV NspI PasI PciI PpuMI
PspOMI PstI PvuII SfcI SmlI StuI StyI TaqII
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AgeI AhdI AlfI
AloI AlwNI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BfrBI BglII BmgBI BmrI
BmtI Bpu10I BsaI BsaWI BseYI BsiEI BsiWI BsmBI
BspEI BspHI BspMI BsrBI BssHII BssSI BstBI BstEII
BstYI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HpaI
KasI KpnI MfeI MluI MmeI MscI NarI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmaI
ZraI