Restriction map for
pF1KB6239
(Linear) (Six-base) MAPSORT of: KIBB6239.seq Check: 4431 from: 1 to: 2253
KIBB6239.orf
With 182 enzymes: SgfI *
January 18, 2013 16:22 ..
AccI GT'mk_AC
Cuts at: 0 1404 2253
Size: 1404 849
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 599 1259 1340 2236 2253
Size: 299 300 660 81 896 17
Fragments arranged by size:
896 660 300 299 81 17
AflIII A'CryG_T
Cuts at: 0 1399 1997 2253
Size: 1399 598 256
Fragments arranged by size:
1399 598 256
AgeI A'CCGG_T
Cuts at: 0 1502 2253
Size: 1502 751
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 737 769 2253
Size: 737 32 1484
Fragments arranged by size:
1484 737 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1171 2253
Size: 1171 1082
ApaLI G'TGCA_C
Cuts at: 0 202 2253
Size: 202 2051
ApoI r'AATT_y
Cuts at: 0 143 354 506 649 665 1245 1635 1921
Size: 143 211 152 143 16 580 390 286
Cuts at: 1921 2063 2253
Size: 142 190
Fragments arranged by size:
580 390 286 211 190 152 143 143
142 16
AseI AT'TA_AT
Cuts at: 0 1124 2253
Size: 1124 1129
BanI G'GyrC_C
Cuts at: 0 1863 2253
Size: 1863 390
BanII G_rGCy'C
Cuts at: 0 226 1234 1843 2253
Size: 226 1008 609 410
Fragments arranged by size:
1008 609 410 226
BbvCI CC'TCA_GC
Cuts at: 0 1460 2253
Size: 1460 793
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2063 2097 2253
Size: 2063 34 156
Fragments arranged by size:
2063 156 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 185 1137 1464 2253
Size: 185 952 327 789
Fragments arranged by size:
952 789 327 185
BclI T'GATC_A
Cuts at: 0 1584 2127 2253
Size: 1584 543 126
Fragments arranged by size:
1584 543 126
BglII A'GATC_T
Cuts at: 0 603 2253
Size: 603 1650
BlpI GC'TnA_GC
Cuts at: 0 1034 2253
Size: 1034 1219
Bme1580I G_kGCm'C
Cuts at: 0 206 706 1618 1729 2253
Size: 206 500 912 111 524
Fragments arranged by size:
912 524 500 206 111
BmgBI CAC'GTC
Cuts at: 0 1875 2253
Size: 1875 378
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 2253
Size: 20 2233
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1433 2253
Size: 628 805 820
Fragments arranged by size:
820 805 628
Bpu10I CC'TnA_GC
Cuts at: 0 1460 2253
Size: 1460 793
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1940 2253
Size: 70 1870 313
Fragments arranged by size:
1870 313 70
BsaI GGTCTCn'nnnn_
Cuts at: 0 344 2253
Size: 344 1909
BsaBI GATnn'nnATC
Cuts at: 0 1894 2253
Size: 1894 359
BsaWI w'CCGG_w
Cuts at: 0 1502 2253
Size: 1502 751
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 395 425 737 767 2253
Size: 395 30 312 30 1486
Fragments arranged by size:
1486 395 312 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1224 1466 1542 2253
Size: 1224 242 76 711
Fragments arranged by size:
1224 711 242 76
BseYI C'CCAG_C
Cuts at: 0 1108 1883 2253
Size: 1108 775 370
Fragments arranged by size:
1108 775 370
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1499 1930 2022 2253
Size: 1499 431 92 231
Fragments arranged by size:
1499 431 231 92
BsiHKAI G_wGCw'C
Cuts at: 0 206 226 1234 2253
Size: 206 20 1008 1019
Fragments arranged by size:
1019 1008 206 20
BsmI GAATG_Cn'
Cuts at: 0 309 531 2253
Size: 309 222 1722
Fragments arranged by size:
1722 309 222
BsmBI CGTCTCn'nnnn_
Cuts at: 0 563 2253
Size: 563 1690
Bsp1286I G_dGCh'C
Cuts at: 0 206 226 706 1234 1618 1729 1843 2253
Size: 206 20 480 528 384 111 114 410
Fragments arranged by size:
528 480 410 384 206 114 111 20
BsrBI CCG'CTC
Cuts at: 0 1038 2253
Size: 1038 1215
BsrDI GCAATG_nn'
Cuts at: 0 226 1373 2253
Size: 226 1147 880
Fragments arranged by size:
1147 880 226
BsrFI r'CCGG_y
Cuts at: 0 1502 2253
Size: 1502 751
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1823 1857 2253
Size: 1823 34 396
Fragments arranged by size:
1823 396 34
BstEII G'GTnAC_C
Cuts at: 0 252 2253
Size: 252 2001
BstXI CCAn_nnnn'nTGG
Cuts at: 0 41 264 2253
Size: 41 223 1989
Fragments arranged by size:
1989 223 41
BstYI r'GATC_y
Cuts at: 0 603 1902 2253
Size: 603 1299 351
Fragments arranged by size:
1299 603 351
BtgI C'CryG_G
Cuts at: 0 1591 2253
Size: 1591 662
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 876 2253
Size: 876 1377
BtsI GCAGTG_nn'
Cuts at: 0 1518 1729 2253
Size: 1518 211 524
Fragments arranged by size:
1518 524 211
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 954 989 2253
Size: 954 35 1264
Fragments arranged by size:
1264 954 35
DraI TTT'AAA
Cuts at: 0 870 2253
Size: 870 1383
EarI CTCTTCn'nnn_
Cuts at: 0 1071 1202 1486 1968 2168 2253
Size: 1071 131 284 482 200 85
Fragments arranged by size:
1071 482 284 200 131 85
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 60 2253
Size: 60 2193
EcoICRI GAG'CTC
Cuts at: 0 224 1232 2253
Size: 224 1008 1021
Fragments arranged by size:
1021 1008 224
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 599 628 1259 1340 1433 2236 2253
Size: 299 300 29 631 81 93 803 17
Fragments arranged by size:
803 631 300 299 93 81 29 17
EcoRI G'AATT_C
Cuts at: 0 1921 2253
Size: 1921 332
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1537 1569 1938 1970 2253
Size: 1537 32 369 32 283
Fragments arranged by size:
1537 369 283 32 32
HincII GTy'rAC
Cuts at: 0 2121 2253
Size: 2121 132
HindIII A'AGCT_T
Cuts at: 0 828 2253
Size: 828 1425
HpaI GTT'AAC
Cuts at: 0 2121 2253
Size: 2121 132
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 2253
Size: 298 1955
MslI CAynn'nnrTG
Cuts at: 0 620 1126 2253
Size: 620 506 1127
Fragments arranged by size:
1127 620 506
MspA1I CmG'CkG
Cuts at: 0 1033 1459 1536 1697 1786 1836 2253
Size: 1033 426 77 161 89 50 417
Fragments arranged by size:
1033 426 417 161 89 77 50
NspI r_CATG'y
Cuts at: 0 72 1403 2001 2253
Size: 72 1331 598 252
Fragments arranged by size:
1331 598 252 72
PciI A'CATG_T
Cuts at: 0 1399 1997 2253
Size: 1399 598 256
Fragments arranged by size:
1399 598 256
PflMI CCAn_nnn'nTGG
Cuts at: 0 996 2253
Size: 996 1257
PfoI T'CCnGG_A
Cuts at: 0 1062 1686 2253
Size: 1062 624 567
Fragments arranged by size:
1062 624 567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 737 769 2253
Size: 737 32 1484
Fragments arranged by size:
1484 737 32
PshAI GACnn'nnGTC
Cuts at: 0 1766 2253
Size: 1766 487
PsiI TTA'TAA
Cuts at: 0 496 932 2253
Size: 496 436 1321
Fragments arranged by size:
1321 496 436
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 111 143 2253
Size: 111 32 2110
Fragments arranged by size:
2110 111 32
PstI C_TGCA'G
Cuts at: 0 1525 1828 2253
Size: 1525 303 425
Fragments arranged by size:
1525 425 303
PvuII CAG'CTG
Cuts at: 0 1536 2253
Size: 1536 717
SacI G_AGCT'C
Cuts at: 0 226 1234 2253
Size: 226 1008 1019
Fragments arranged by size:
1019 1008 226
SapI GCTCTTCn'nnn_
Cuts at: 0 2168 2253
Size: 2168 85
SfcI C'TryA_G
Cuts at: 0 346 855 1521 1824 2253
Size: 346 509 666 303 429
Fragments arranged by size:
666 509 429 346 303
SmlI C'TyrA_G
Cuts at: 0 85 1955 2253
Size: 85 1870 298
Fragments arranged by size:
1870 298 85
SspI AAT'ATT
Cuts at: 0 1284 2253
Size: 1284 969
StyI C'CwwG_G
Cuts at: 0 531 1480 2253
Size: 531 949 773
Fragments arranged by size:
949 773 531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 936 2253
Size: 936 1317
TatI w'GTAC_w
Cuts at: 0 65 468 2253
Size: 65 403 1785
Fragments arranged by size:
1785 403 65
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 269 301 2253
Size: 269 32 1952
Fragments arranged by size:
1952 269 32
XbaI T'CTAG_A
Cuts at: 0 957 2253
Size: 957 1296
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1128 2253
Size: 1128 1125
Enzymes that do cut:
AccI AcuI AflIII AgeI AloI AlwNI ApaLI ApoI
AseI BanI BanII BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
BsmBI Bsp1286I BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI CspCI DraI EarI EciI
EcoICRI Eco57MI EcoRI Hin4I HincII HindIII HpaI MmeI
MslI MspA1I NspI PciI PflMI PfoI PpiI PshAI
PsiI PsrI PstI PvuII SacI SapI SfcI SmlI
SspI StyI TaqII TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AhdI AleI
AlfI ApaI AscI AsiSI AvaI AvrII BaeI BamHI
BbeI BbsI BfrBI BglI BmtI BplI BsaAI BsaHI
BsiEI BsiWI BspEI BspHI BspMI BsrGI BssHII BssSI
BstBI BstZ17I Bsu36I ClaI DraIII DrdI EaeI EagI
EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpuMI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XhoI XmaI XmnI ZraI