Restriction map for
pF1KB6237
(Linear) (Six-base) MAPSORT of: KIBB6237.seq Check: 3437 from: 1 to: 2253
KIBB6237.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
Acc65I G'GTAC_C
Cuts at: 0 286 2253
Size: 286 1967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 371 759 1263 1431 1683 2187 2253
Size: 371 388 504 168 252 504 66
Fragments arranged by size:
504 504 388 371 252 168 66
AlwNI CAG_nnn'CTG
Cuts at: 0 340 477 1454 2238 2253
Size: 340 137 977 784 15
Fragments arranged by size:
977 784 340 137 15
ApaI G_GGCC'C
Cuts at: 0 1205 2253
Size: 1205 1048
ApoI r'AATT_y
Cuts at: 0 544 633 809 893 977 1061 1145 1397
Size: 544 89 176 84 84 84 84 252
Cuts at: 1397 1481 1649 1817 1985 2153 2253
Size: 84 168 168 168 168 100
Fragments arranged by size:
544 252 176 168 168 168 168 100
89 84 84 84 84 84
BanI G'GyrC_C
Cuts at: 0 286 2253
Size: 286 1967
BanII G_rGCy'C
Cuts at: 0 1205 1963 2253
Size: 1205 758 290
Fragments arranged by size:
1205 758 290
BclI T'GATC_A
Cuts at: 0 840 2253
Size: 840 1413
BglI GCCn_nnn'nGGC
Cuts at: 0 1037 2253
Size: 1037 1216
BglII A'GATC_T
Cuts at: 0 6 2253
Size: 6 2247
BlpI GC'TnA_GC
Cuts at: 0 1637 2253
Size: 1637 616
Bme1580I G_kGCm'C
Cuts at: 0 1205 2253
Size: 1205 1048
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 309 509 637 1331 2003 2253
Size: 228 81 200 128 694 672 250
Fragments arranged by size:
694 672 250 228 200 128 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1646 2253
Size: 1646 607
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 350 581 755 839 923 1175 1427
Size: 2 348 231 174 84 84 252 252
Cuts at: 1427 1679 1763 2099 2253
Size: 252 84 336 154
Fragments arranged by size:
348 336 252 252 252 231 174 154
84 84 84 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1559 2253
Size: 1559 694
BsaI GGTCTCn'nnnn_
Cuts at: 0 198 598 2253
Size: 198 400 1655
Fragments arranged by size:
1655 400 198
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 1364 2253
Size: 314 1050 889
Fragments arranged by size:
1050 889 314
BsiHKAI G_wGCw'C
Cuts at: 0 1123 1963 2047 2131 2253
Size: 1123 840 84 84 122
Fragments arranged by size:
1123 840 122 84 84
BsiWI C'GTAC_G
Cuts at: 0 1250 2253
Size: 1250 1003
BsmI GAATG_Cn'
Cuts at: 0 275 1261 1513 1780 2110 2253
Size: 275 986 252 267 330 143
Fragments arranged by size:
986 330 275 267 252 143
Bsp1286I G_dGCh'C
Cuts at: 0 1123 1205 1963 2047 2131 2253
Size: 1123 82 758 84 84 122
Fragments arranged by size:
1123 758 122 84 84 82
BspHI T'CATG_A
Cuts at: 0 1680 2253
Size: 1680 573
BsrDI GCAATG_nn'
Cuts at: 0 678 1266 1434 2022 2253
Size: 678 588 168 588 231
Fragments arranged by size:
678 588 588 231 168
BssSI C'ACGA_G
Cuts at: 0 1488 2253
Size: 1488 765
BstEII G'GTnAC_C
Cuts at: 0 495 2253
Size: 495 1758
BstYI r'GATC_y
Cuts at: 0 6 1733 2253
Size: 6 1727 520
Fragments arranged by size:
1727 520 6
BtgI C'CryG_G
Cuts at: 0 256 2253
Size: 256 1997
BtsI GCAGTG_nn'
Cuts at: 0 475 1518 2253
Size: 475 1043 735
Fragments arranged by size:
1043 735 475
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 660 695 1248 1283 1416 1451 2253
Size: 660 35 553 35 133 35 802
Fragments arranged by size:
802 660 553 133 35 35 35
DraI TTT'AAA
Cuts at: 0 1086 2253
Size: 1086 1167
EaeI y'GGCC_r
Cuts at: 0 141 2253
Size: 141 2112
EarI CTCTTCn'nnn_
Cuts at: 0 350 596 2253
Size: 350 246 1657
Fragments arranged by size:
1657 350 246
EcoICRI GAG'CTC
Cuts at: 0 1961 2253
Size: 1961 292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 350 371 581 755 759 839 923
Size: 2 348 21 210 174 4 80 84
Cuts at: 923 1175 1263 1427 1431 1679 1683 1763 2099
Size: 252 88 164 4 248 4 80 336
Cuts at: 2099 2187 2253
Size: 88 66
Fragments arranged by size:
348 336 252 248 210 174 164 88
88 84 80 80 66 21 4 4
4 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1113 2253
Size: 1113 1140
EcoO109I rG'GnC_Cy
Cuts at: 0 529 953 1202 2253
Size: 529 424 249 1051
Fragments arranged by size:
1051 529 424 249
EcoRI G'AATT_C
Cuts at: 0 633 809 893 977 1481 1649 1817 1985
Size: 633 176 84 84 504 168 168 168
Cuts at: 1985 2153 2253
Size: 168 100
Fragments arranged by size:
633 504 176 168 168 168 168 100
84 84
EcoRV GAT'ATC
Cuts at: 0 2209 2253
Size: 2209 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 856 888 942 974 1950 1982 2034
Size: 30 826 32 54 32 976 32 52
Cuts at: 2034 2046 2066 2078 2118 2150 2226 2253
Size: 12 20 12 40 32 76 27
Fragments arranged by size:
976 826 76 54 52 40 32 32
32 32 30 27 20 12 12
HindIII A'AGCT_T
Cuts at: 0 1583 1610 2253
Size: 1583 27 643
Fragments arranged by size:
1583 643 27
KpnI G_GTAC'C
Cuts at: 0 290 2253
Size: 290 1963
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 2253
Size: 437 1816
MscI TGG'CCA
Cuts at: 0 143 2253
Size: 143 2110
MslI CAynn'nnrTG
Cuts at: 0 834 918 1238 1685 2253
Size: 834 84 320 447 568
Fragments arranged by size:
834 568 447 320 84
MspA1I CmG'CkG
Cuts at: 0 132 2253
Size: 132 2121
NcoI C'CATG_G
Cuts at: 0 256 2253
Size: 256 1997
NdeI CA'TA_TG
Cuts at: 0 1226 2253
Size: 1226 1027
PflMI CCAn_nnn'nTGG
Cuts at: 0 1047 2253
Size: 1047 1206
PpuMI rG'GwC_Cy
Cuts at: 0 953 2253
Size: 953 1300
PsiI TTA'TAA
Cuts at: 0 796 2093 2253
Size: 796 1297 160
Fragments arranged by size:
1297 796 160
PspOMI G'GGCC_C
Cuts at: 0 1201 2253
Size: 1201 1052
PvuII CAG'CTG
Cuts at: 0 132 2253
Size: 132 2121
SacI G_AGCT'C
Cuts at: 0 1963 2253
Size: 1963 290
SapI GCTCTTCn'nnn_
Cuts at: 0 350 2253
Size: 350 1903
SexAI A'CCwGG_T
Cuts at: 0 188 2253
Size: 188 2065
SfcI C'TryA_G
Cuts at: 0 197 2253
Size: 197 2056
SmlI C'TyrA_G
Cuts at: 0 1574 2253
Size: 1574 679
StuI AGG'CCT
Cuts at: 0 1192 1444 2253
Size: 1192 252 809
Fragments arranged by size:
1192 809 252
StyI C'CwwG_G
Cuts at: 0 36 256 2253
Size: 36 220 1997
Fragments arranged by size:
1997 220 36
XmnI GAAnn'nnTTC
Cuts at: 0 1276 1649 1696 2157 2253
Size: 1276 373 47 461 96
Fragments arranged by size:
1276 461 373 96 47
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI ApoI BanI BanII BclI
BglI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsgI BsiHKAI BsiWI BsmI Bsp1286I BspHI BsrDI
BssSI BstEII BstYI BtgI BtsI CspCI DraI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I
HindIII KpnI MmeI MscI MslI MspA1I NcoI NdeI
PflMI PpuMI PsiI PspOMI PvuII SacI SapI SexAI
SfcI SmlI StuI StyI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BfrBI BmgBI BmtI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsmBI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI BstZ17I
Bsu36I BtgZI ClaI DraIII DrdI EagI EciI FalI
FseI FspI FspAI HaeII HincII HpaI KasI MfeI
MluI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI