Restriction map for
pF1KB6235
(Linear) (Six-base) MAPSORT of: KIBB6235.seq Check: 2453 from: 1 to: 2247
KIBB6235.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 75 2247
Size: 75 2172
AatII G_ACGT'C
Cuts at: 0 1340 2247
Size: 1340 907
AccI GT'mk_AC
Cuts at: 0 1224 1586 1881 2247
Size: 1224 362 295 366
Fragments arranged by size:
1224 366 362 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1522 1663 2045 2247
Size: 1522 141 382 202
Fragments arranged by size:
1522 382 202 141
AfeI AGC'GCT
Cuts at: 0 2220 2247
Size: 2220 27
AflIII A'CryG_T
Cuts at: 0 150 1496 2247
Size: 150 1346 751
Fragments arranged by size:
1346 751 150
AhdI GACnn_n'nnGTC
Cuts at: 0 513 2247
Size: 513 1734
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1724 2247
Size: 1724 523
AlwNI CAG_nnn'CTG
Cuts at: 0 16 558 1485 1791 2247
Size: 16 542 927 306 456
Fragments arranged by size:
927 542 456 306 16
ApoI r'AATT_y
Cuts at: 0 426 1380 2247
Size: 426 954 867
Fragments arranged by size:
954 867 426
AseI AT'TA_AT
Cuts at: 0 682 2247
Size: 682 1565
AvaI C'yCGr_G
Cuts at: 0 455 980 2134 2247
Size: 455 525 1154 113
Fragments arranged by size:
1154 525 455 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 806 1764 2247
Size: 806 958 483
Fragments arranged by size:
958 806 483
BciVI GTATCCnnnnn_n'
Cuts at: 0 239 2247
Size: 239 2008
BfrBI ATG'CAT
Cuts at: 0 976 2247
Size: 976 1271
BlpI GC'TnA_GC
Cuts at: 0 2095 2247
Size: 2095 152
Bme1580I G_kGCm'C
Cuts at: 0 343 727 2247
Size: 343 384 1520
Fragments arranged by size:
1520 384 343
BmgBI CAC'GTC
Cuts at: 0 1499 2247
Size: 1499 748
BmrI ACTGGGnnnn_n'
Cuts at: 0 26 649 1757 2247
Size: 26 623 1108 490
Fragments arranged by size:
1108 623 490 26
BmtI G_CTAG'C
Cuts at: 0 1856 2247
Size: 1856 391
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 394 2247
Size: 394 1853
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 413 535 1717 2247
Size: 287 126 122 1182 530
Fragments arranged by size:
1182 530 287 126 122
Bpu10I CC'TnA_GC
Cuts at: 0 164 1928 2247
Size: 164 1764 319
Fragments arranged by size:
1764 319 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 2054 2247
Size: 272 1782 193
Fragments arranged by size:
1782 272 193
BsaI GGTCTCn'nnnn_
Cuts at: 0 324 2247
Size: 324 1923
BsaBI GATnn'nnATC
Cuts at: 0 1327 2247
Size: 1327 920
BsaHI Gr'CG_yC
Cuts at: 0 362 1337 2247
Size: 362 975 910
Fragments arranged by size:
975 910 362
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2141 2171 2247
Size: 2141 30 76
Fragments arranged by size:
2141 76 30
BseYI C'CCAG_C
Cuts at: 0 465 2247
Size: 465 1782
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 190 1550 2235 2247
Size: 157 33 1360 685 12
Fragments arranged by size:
1360 685 157 33 12
BsiHKAI G_wGCw'C
Cuts at: 0 987 1079 1693 2183 2247
Size: 987 92 614 490 64
Fragments arranged by size:
987 614 490 92 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 484 2247
Size: 484 1763
Bsp1286I G_dGCh'C
Cuts at: 0 343 727 987 1079 1693 2183 2247
Size: 343 384 260 92 614 490 64
Fragments arranged by size:
614 490 384 343 260 92 64
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 75 114 132 2247
Size: 75 39 18 2115
Fragments arranged by size:
2115 75 39 18
BsrBI CCG'CTC
Cuts at: 0 163 2247
Size: 163 2084
BsrGI T'GTAC_A
Cuts at: 0 251 611 2247
Size: 251 360 1636
Fragments arranged by size:
1636 360 251
BssSI C'ACGA_G
Cuts at: 0 1526 2247
Size: 1526 721
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1528 1791 2247
Size: 1528 263 456
Fragments arranged by size:
1528 456 263
BstBI TT'CG_AA
Cuts at: 0 1377 2247
Size: 1377 870
BstXI CCAn_nnnn'nTGG
Cuts at: 0 265 720 754 2247
Size: 265 455 34 1493
Fragments arranged by size:
1493 455 265 34
BtgI C'CryG_G
Cuts at: 0 314 1296 2247
Size: 314 982 951
Fragments arranged by size:
982 951 314
BtsI GCAGTG_nn'
Cuts at: 0 80 1054 1538 1708 1851 2025 2237 2247
Size: 80 974 484 170 143 174 212 10
Fragments arranged by size:
974 484 212 174 170 143 80 10
DraI TTT'AAA
Cuts at: 0 1979 2247
Size: 1979 268
DraIII CAC_nnn'GTG
Cuts at: 0 441 2247
Size: 441 1806
EarI CTCTTCn'nnn_
Cuts at: 0 539 1020 1682 2064 2247
Size: 539 481 662 382 183
Fragments arranged by size:
662 539 481 382 183
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 288 2247
Size: 288 1959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 287 413 535 1522 1663 1717 2045 2247
Size: 287 126 122 987 141 54 328 202
Fragments arranged by size:
987 328 287 202 141 126 122 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1516 2247
Size: 1516 731
EcoRI G'AATT_C
Cuts at: 0 426 2247
Size: 426 1821
EcoRV GAT'ATC
Cuts at: 0 1047 2247
Size: 1047 1200
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1423 2247
Size: 1423 824
HaeII r_GCGC'y
Cuts at: 0 1155 2222 2247
Size: 1155 1067 25
Fragments arranged by size:
1155 1067 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 4 16 36 48 365 397 499 531
Size: 4 12 20 12 317 32 102 32
Cuts at: 531 1036 1068 2247
Size: 505 32 1179
Fragments arranged by size:
1179 505 317 102 32 32 32 20
12 12 4
HincII GTy'rAC
Cuts at: 0 907 1225 1587 2247
Size: 907 318 362 660
Fragments arranged by size:
907 660 362 318
MfeI C'AATT_G
Cuts at: 0 473 2247
Size: 473 1774
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 542 1195 2247
Size: 2 540 653 1052
Fragments arranged by size:
1052 653 540 2
MslI CAynn'nnrTG
Cuts at: 0 752 2247
Size: 752 1495
MspA1I CmG'CkG
Cuts at: 0 1462 2247
Size: 1462 785
NcoI C'CATG_G
Cuts at: 0 1296 2247
Size: 1296 951
NheI G'CTAG_C
Cuts at: 0 1852 2247
Size: 1852 395
NsiI A_TGCA'T
Cuts at: 0 978 2247
Size: 978 1269
NspI r_CATG'y
Cuts at: 0 154 807 2124 2247
Size: 154 653 1317 123
Fragments arranged by size:
1317 653 154 123
PciI A'CATG_T
Cuts at: 0 150 2247
Size: 150 2097
PflMI CCAn_nnn'nTGG
Cuts at: 0 333 997 1177 1314 2247
Size: 333 664 180 137 933
Fragments arranged by size:
933 664 333 180 137
PfoI T'CCnGG_A
Cuts at: 0 1601 1992 2247
Size: 1601 391 255
Fragments arranged by size:
1601 391 255
PstI C_TGCA'G
Cuts at: 0 1061 2244 2247
Size: 1061 1183 3
Fragments arranged by size:
1183 1061 3
SalI G'TCGA_C
Cuts at: 0 1223 1585 2247
Size: 1223 362 662
Fragments arranged by size:
1223 662 362
ScaI AGT'ACT
Cuts at: 0 2196 2247
Size: 2196 51
SfcI C'TryA_G
Cuts at: 0 1057 1809 1949 2222 2240 2247
Size: 1057 752 140 273 18 7
Fragments arranged by size:
1057 752 273 140 18 7
SmlI C'TyrA_G
Cuts at: 0 287 980 2069 2134 2247
Size: 287 693 1089 65 113
Fragments arranged by size:
1089 693 287 113 65
SphI G_CATG'C
Cuts at: 0 2124 2247
Size: 2124 123
SspI AAT'ATT
Cuts at: 0 1091 1428 2247
Size: 1091 337 819
Fragments arranged by size:
1091 819 337
StyI C'CwwG_G
Cuts at: 0 1296 1451 2247
Size: 1296 155 796
Fragments arranged by size:
1296 796 155
SwaI ATTT'AAAT
Cuts at: 0 1979 2247
Size: 1979 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1608 2247
Size: 1608 639
TatI w'GTAC_w
Cuts at: 0 105 251 611 2194 2247
Size: 105 146 360 1583 53
Fragments arranged by size:
1583 360 146 105 53
Tth111I GACn'n_nGTC
Cuts at: 0 18 632 655 2247
Size: 18 614 23 1592
Fragments arranged by size:
1592 614 23 18
XhoI C'TCGA_G
Cuts at: 0 980 2134 2247
Size: 980 1154 113
Fragments arranged by size:
1154 980 113
ZraI GAC'GTC
Cuts at: 0 1338 2247
Size: 1338 909
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AlfI
AlwNI ApoI AseI AvaI BbsI BciVI BfrBI BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrGI BssSI BstAPI BstBI BstXI
BtgI BtsI DraI DraIII EarI EciI Eco57MI EcoNI
EcoRI EcoRV FalI HaeII Hin4I HincII MfeI MmeI
MslI MspA1I NcoI NheI NsiI NspI PciI PflMI
PfoI PstI SalI ScaI SfcI SmlI SphI SspI
StyI SwaI TaqII TatI Tth111I XhoI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AleI AloI ApaI ApaLI
AscI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BclI BglI BglII BsaAI BsaWI BseRI
BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrFI BssHII
BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DrdI
EaeI EagI EcoICRI EcoO109I FseI FspI FspAI HindIII
HpaI KasI KpnI MluI MscI NaeI NarI NdeI
NgoMIV NotI NruI PacI PasI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
TstI XbaI XcmI XmaI XmnI