Restriction map for
pF1KB6234
(Linear) (Six-base) MAPSORT of: KIBB6234.seq Check: 5009 from: 1 to: 2031
KIBB6234.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 770 2031
Size: 770 1261
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 810 1318 1370 2031
Size: 668 142 508 52 661
Fragments arranged by size:
668 661 508 142 52
AfeI AGC'GCT
Cuts at: 0 1410 2031
Size: 1410 621
AflIII A'CryG_T
Cuts at: 0 383 1050 2031
Size: 383 667 981
Fragments arranged by size:
981 667 383
AleI CACnn'nnGTG
Cuts at: 0 214 817 2031
Size: 214 603 1214
Fragments arranged by size:
1214 603 214
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1180 2031
Size: 1180 851
AlwNI CAG_nnn'CTG
Cuts at: 0 1775 2031
Size: 1775 256
ApaI G_GGCC'C
Cuts at: 0 620 1176 2031
Size: 620 556 855
Fragments arranged by size:
855 620 556
ApaLI G'TGCA_C
Cuts at: 0 352 694 2031
Size: 352 342 1337
Fragments arranged by size:
1337 352 342
ApoI r'AATT_y
Cuts at: 0 1384 2031
Size: 1384 647
AvaI C'yCGr_G
Cuts at: 0 942 2031
Size: 942 1089
BanI G'GyrC_C
Cuts at: 0 55 1492 1972 2031
Size: 55 1437 480 59
Fragments arranged by size:
1437 480 59 55
BanII G_rGCy'C
Cuts at: 0 620 682 1176 1337 2031
Size: 620 62 494 161 694
Fragments arranged by size:
694 620 494 161 62
BbeI G_GCGC'C
Cuts at: 0 1496 2031
Size: 1496 535
BbsI GAAGACnn'nnnn_
Cuts at: 0 1847 2031
Size: 1847 184
BbvCI CC'TCA_GC
Cuts at: 0 461 2031
Size: 461 1570
BclI T'GATC_A
Cuts at: 0 320 2031
Size: 320 1711
BfrBI ATG'CAT
Cuts at: 0 1420 2031
Size: 1420 611
BglI GCCn_nnn'nGGC
Cuts at: 0 435 836 1111 2031
Size: 435 401 275 920
Fragments arranged by size:
920 435 401 275
Bme1580I G_kGCm'C
Cuts at: 0 60 356 620 698 1176 2031
Size: 60 296 264 78 478 855
Fragments arranged by size:
855 478 296 264 78 60
BmrI ACTGGGnnnn_n'
Cuts at: 0 735 1492 2031
Size: 735 757 539
Fragments arranged by size:
757 735 539
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1193 2016 2031
Size: 1193 823 15
Fragments arranged by size:
1193 823 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 471 1105 1378 2031
Size: 178 293 634 273 653
Fragments arranged by size:
653 634 293 273 178
Bpu10I CC'TnA_GC
Cuts at: 0 61 461 1960 2031
Size: 61 400 1499 71
Fragments arranged by size:
1499 400 71 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1252 1321 2031
Size: 1252 69 710
Fragments arranged by size:
1252 710 69
BsaI GGTCTCn'nnnn_
Cuts at: 0 17 367 386 1696 2031
Size: 17 350 19 1310 335
Fragments arranged by size:
1310 350 335 19 17
BsaAI yAC'GTr
Cuts at: 0 750 2031
Size: 750 1281
BsaHI Gr'CG_yC
Cuts at: 0 1493 2031
Size: 1493 538
BsaWI w'CCGG_w
Cuts at: 0 726 1389 1946 2031
Size: 726 663 557 85
Fragments arranged by size:
726 663 557 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 547 577 1663 1693 2031
Size: 547 30 1086 30 338
Fragments arranged by size:
1086 547 338 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 930 1483 2031
Size: 930 553 548
Fragments arranged by size:
930 553 548
BseYI C'CCAG_C
Cuts at: 0 108 304 1768 2026 2031
Size: 108 196 1464 258 5
Fragments arranged by size:
1464 258 196 108 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 755 991 1186 2031
Size: 214 541 236 195 845
Fragments arranged by size:
845 541 236 214 195
BsiEI CG_ry'CG
Cuts at: 0 907 2031
Size: 907 1124
BsiHKAI G_wGCw'C
Cuts at: 0 356 698 1337 1727 2031
Size: 356 342 639 390 304
Fragments arranged by size:
639 390 356 342 304
BsmI GAATG_Cn'
Cuts at: 0 1911 2031
Size: 1911 120
Bsp1286I G_dGCh'C
Cuts at: 0 60 356 620 682 698 1176 1337 1727
Size: 60 296 264 62 16 478 161 390
Cuts at: 1727 2031
Size: 304
Fragments arranged by size:
478 390 304 296 264 161 62 60
16
BspEI T'CCGG_A
Cuts at: 0 726 1389 2031
Size: 726 663 642
Fragments arranged by size:
726 663 642
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 745 770 2031
Size: 745 25 1261
Fragments arranged by size:
1261 745 25
BsrBI CCG'CTC
Cuts at: 0 51 72 2031
Size: 51 21 1959
Fragments arranged by size:
1959 51 21
BsrDI GCAATG_nn'
Cuts at: 0 1026 2031
Size: 1026 1005
BsrFI r'CCGG_y
Cuts at: 0 710 2021 2031
Size: 710 1311 10
Fragments arranged by size:
1311 710 10
BssSI C'ACGA_G
Cuts at: 0 1515 2031
Size: 1515 516
BstEII G'GTnAC_C
Cuts at: 0 757 1237 2031
Size: 757 480 794
Fragments arranged by size:
794 757 480
BstXI CCAn_nnnn'nTGG
Cuts at: 0 388 1713 2031
Size: 388 1325 318
Fragments arranged by size:
1325 388 318
Bsu36I CC'TnA_GG
Cuts at: 0 1255 2031
Size: 1255 776
BtgI C'CryG_G
Cuts at: 0 1610 2031
Size: 1610 421
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 402 2031
Size: 402 1629
BtsI GCAGTG_nn'
Cuts at: 0 1135 1596 1971 2023 2031
Size: 1135 461 375 52 8
Fragments arranged by size:
1135 461 375 52 8
ClaI AT'CG_AT
Cuts at: 0 1799 2031
Size: 1799 232
EaeI y'GGCC_r
Cuts at: 0 543 904 1593 1784 2031
Size: 543 361 689 191 247
Fragments arranged by size:
689 543 361 247 191
EagI C'GGCC_G
Cuts at: 0 904 2031
Size: 904 1127
EarI CTCTTCn'nnn_
Cuts at: 0 1453 2031
Size: 1453 578
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 466 1492 2031
Size: 466 1026 539
Fragments arranged by size:
1026 539 466
EcoICRI GAG'CTC
Cuts at: 0 1335 2031
Size: 1335 696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 471 668 810 1105 1318 1370 1378
Size: 178 293 197 142 295 213 52 8
Cuts at: 1378 2031
Size: 653
Fragments arranged by size:
653 295 293 213 197 178 142 52
8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 624 2031
Size: 624 1407
EcoO109I rG'GnC_Cy
Cuts at: 0 222 617 1172 1173 1480 1979 2031
Size: 222 395 555 1 307 499 52
Fragments arranged by size:
555 499 395 307 222 52 1
EcoRV GAT'ATC
Cuts at: 0 1617 2031
Size: 1617 414
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1323 2031
Size: 1323 708
HaeII r_GCGC'y
Cuts at: 0 78 430 1412 1496 2031
Size: 78 352 982 84 535
Fragments arranged by size:
982 535 352 84 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 1519 1551 1663 1695 2031
Size: 14 32 1473 32 112 32 336
Fragments arranged by size:
1473 336 112 32 32 32 14
HincII GTy'rAC
Cuts at: 0 12 150 576 855 1749 2031
Size: 12 138 426 279 894 282
Fragments arranged by size:
894 426 282 279 138 12
HindIII A'AGCT_T
Cuts at: 0 772 2031
Size: 772 1259
KasI G'GCGC_C
Cuts at: 0 1492 2031
Size: 1492 539
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1010 1898 2031
Size: 1010 888 133
Fragments arranged by size:
1010 888 133
MscI TGG'CCA
Cuts at: 0 545 1786 2031
Size: 545 1241 245
Fragments arranged by size:
1241 545 245
MslI CAynn'nnrTG
Cuts at: 0 214 382 643 817 1019 1247 2031
Size: 214 168 261 174 202 228 784
Fragments arranged by size:
784 261 228 214 202 174 168
MspA1I CmG'CkG
Cuts at: 0 228 549 614 688 903 1322 1441 1896
Size: 228 321 65 74 215 419 119 455
Cuts at: 1896 2031
Size: 135
Fragments arranged by size:
455 419 321 228 215 135 119 74
65
NaeI GCC'GGC
Cuts at: 0 2023 2031
Size: 2023 8
NarI GG'CG_CC
Cuts at: 0 1493 2031
Size: 1493 538
NgoMIV G'CCGG_C
Cuts at: 0 2021 2031
Size: 2021 10
NsiI A_TGCA'T
Cuts at: 0 1422 2031
Size: 1422 609
NspI r_CATG'y
Cuts at: 0 352 387 1054 2031
Size: 352 35 667 977
Fragments arranged by size:
977 667 352 35
PciI A'CATG_T
Cuts at: 0 383 1050 2031
Size: 383 667 981
Fragments arranged by size:
981 667 383
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 2031
Size: 552 1479
PpuMI rG'GwC_Cy
Cuts at: 0 222 1480 2031
Size: 222 1258 551
Fragments arranged by size:
1258 551 222
PspOMI G'GGCC_C
Cuts at: 0 616 1172 2031
Size: 616 556 859
Fragments arranged by size:
859 616 556
PstI C_TGCA'G
Cuts at: 0 131 1760 2031
Size: 131 1629 271
Fragments arranged by size:
1629 271 131
PvuII CAG'CTG
Cuts at: 0 549 1441 2031
Size: 549 892 590
Fragments arranged by size:
892 590 549
SacI G_AGCT'C
Cuts at: 0 1337 2031
Size: 1337 694
SanDI GG'GwC_CC
Cuts at: 0 222 2031
Size: 222 1809
ScaI AGT'ACT
Cuts at: 0 538 2031
Size: 538 1493
SexAI A'CCwGG_T
Cuts at: 0 1482 1712 2031
Size: 1482 230 319
Fragments arranged by size:
1482 319 230
SfcI C'TryA_G
Cuts at: 0 127 1756 2031
Size: 127 1629 275
Fragments arranged by size:
1629 275 127
SfoI GGC'GCC
Cuts at: 0 1494 2031
Size: 1494 537
SmlI C'TyrA_G
Cuts at: 0 1267 1336 2031
Size: 1267 69 695
Fragments arranged by size:
1267 695 69
SnaBI TAC'GTA
Cuts at: 0 750 2031
Size: 750 1281
SpeI A'CTAG_T
Cuts at: 0 849 2031
Size: 849 1182
StyI C'CwwG_G
Cuts at: 0 1728 2031
Size: 1728 303
TatI w'GTAC_w
Cuts at: 0 536 2031
Size: 536 1495
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 2031
Size: 547 32 1452
Fragments arranged by size:
1452 547 32
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI
BclI BfrBI BglI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssSI BstEII BstXI Bsu36I BtgI BtgZI
BtsI ClaI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NsiI NspI PciI PflMI PpuMI PspOMI PstI PvuII
SacI SanDI ScaI SexAI SfcI SfoI SmlI SnaBI
SpeI StyI TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BglII BlpI BmgBI BmtI BsaBI BsiWI BsmBI BspHI
BsrGI BssHII BstAPI BstBI BstYI BstZ17I CspCI DraI
DraIII DrdI EcoRI FseI FspI FspAI HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI PacI
PasI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI SfiI
SgfI SgrAI SmaI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI