Restriction map for
pF1KB6214
(Linear) (Six-base) MAPSORT of: KIBB6214.seq Check: 8277 from: 1 to: 2175
KIBB6214.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
Acc65I G'GTAC_C
Cuts at: 0 1368 2175
Size: 1368 807
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 455 571 1757 2175
Size: 259 196 116 1186 418
Fragments arranged by size:
1186 418 259 196 116
AleI CACnn'nnGTG
Cuts at: 0 1588 2175
Size: 1588 587
ApaI G_GGCC'C
Cuts at: 0 197 2175
Size: 197 1978
ApoI r'AATT_y
Cuts at: 0 1333 1560 2175
Size: 1333 227 615
Fragments arranged by size:
1333 615 227
AvaI C'yCGr_G
Cuts at: 0 422 2175
Size: 422 1753
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 961 994 2175
Size: 961 33 1181
Fragments arranged by size:
1181 961 33
BanI G'GyrC_C
Cuts at: 0 1368 2175
Size: 1368 807
BanII G_rGCy'C
Cuts at: 0 197 2175
Size: 197 1978
BbsI GAAGACnn'nnnn_
Cuts at: 0 1181 2175
Size: 1181 994
BciVI GTATCCnnnnn_n'
Cuts at: 0 471 1779 1959 2175
Size: 471 1308 180 216
Fragments arranged by size:
1308 471 216 180
Bme1580I G_kGCm'C
Cuts at: 0 197 2175
Size: 197 1978
BmrI ACTGGGnnnn_n'
Cuts at: 0 440 786 2175
Size: 440 346 1389
Fragments arranged by size:
1389 440 346
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 2175
Size: 81 2094
Bpu10I CC'TnA_GC
Cuts at: 0 1625 2175
Size: 1625 550
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 1241 1467 2175
Size: 50 1191 226 708
Fragments arranged by size:
1191 708 226 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 1525 2175
Size: 1525 650
BsaBI GATnn'nnATC
Cuts at: 0 1919 2175
Size: 1919 256
BsaWI w'CCGG_w
Cuts at: 0 553 2175
Size: 553 1622
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 844 874 1448 1478 1627 1657 2175
Size: 844 30 574 30 149 30 518
Fragments arranged by size:
844 574 518 149 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1635 1644 2175
Size: 1635 9 531
Fragments arranged by size:
1635 531 9
BseYI C'CCAG_C
Cuts at: 0 756 2175
Size: 756 1419
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2035 2175
Size: 2035 140
BsiHKAI G_wGCw'C
Cuts at: 0 66 2175
Size: 66 2109
BsmI GAATG_Cn'
Cuts at: 0 563 2175
Size: 563 1612
Bsp1286I G_dGCh'C
Cuts at: 0 66 197 2175
Size: 66 131 1978
Fragments arranged by size:
1978 131 66
BspHI T'CATG_A
Cuts at: 0 1137 2175
Size: 1137 1038
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 905 2175
Size: 905 1270
BsrDI GCAATG_nn'
Cuts at: 0 532 1937 2175
Size: 532 1405 238
Fragments arranged by size:
1405 532 238
BsrGI T'GTAC_A
Cuts at: 0 1104 2175
Size: 1104 1071
BssSI C'ACGA_G
Cuts at: 0 152 2175
Size: 152 2023
BstEII G'GTnAC_C
Cuts at: 0 1897 2175
Size: 1897 278
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1590 2175
Size: 1590 585
Bsu36I CC'TnA_GG
Cuts at: 0 392 2175
Size: 392 1783
BtgI C'CryG_G
Cuts at: 0 660 1892 2175
Size: 660 1232 283
Fragments arranged by size:
1232 660 283
DraIII CAC_nnn'GTG
Cuts at: 0 727 2175
Size: 727 1448
DrdI GACnn_nn'nnGTC
Cuts at: 0 744 2175
Size: 744 1431
EarI CTCTTCn'nnn_
Cuts at: 0 41 431 590 1196 2175
Size: 41 390 159 606 979
Fragments arranged by size:
979 606 390 159 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 81 259 455 571 1757 2175
Size: 81 178 196 116 1186 418
Fragments arranged by size:
1186 418 196 178 116 81
EcoO109I rG'GnC_Cy
Cuts at: 0 193 194 580 737 2175
Size: 193 1 386 157 1438
Fragments arranged by size:
1438 386 193 157 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 589 935 1086 2175
Size: 589 346 151 1089
Fragments arranged by size:
1089 589 346 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 198 230 375 407 2032 2064 2175
Size: 198 32 145 32 1625 32 111
Fragments arranged by size:
1625 198 145 111 32 32 32
HincII GTy'rAC
Cuts at: 0 30 2175
Size: 30 2145
HindIII A'AGCT_T
Cuts at: 0 1046 2175
Size: 1046 1129
KpnI G_GTAC'C
Cuts at: 0 1372 2175
Size: 1372 803
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1423 1932 2175
Size: 1423 509 243
Fragments arranged by size:
1423 509 243
MslI CAynn'nnrTG
Cuts at: 0 612 1427 1588 2086 2175
Size: 612 815 161 498 89
Fragments arranged by size:
815 612 498 161 89
MspA1I CmG'CkG
Cuts at: 0 1735 2175
Size: 1735 440
NcoI C'CATG_G
Cuts at: 0 1892 2175
Size: 1892 283
NdeI CA'TA_TG
Cuts at: 0 804 2175
Size: 804 1371
NspI r_CATG'y
Cuts at: 0 1059 1862 2175
Size: 1059 803 313
Fragments arranged by size:
1059 803 313
PflMI CCAn_nnn'nTGG
Cuts at: 0 240 808 2175
Size: 240 568 1367
Fragments arranged by size:
1367 568 240
PpuMI rG'GwC_Cy
Cuts at: 0 737 2175
Size: 737 1438
PspOMI G'GGCC_C
Cuts at: 0 193 2175
Size: 193 1982
PstI C_TGCA'G
Cuts at: 0 225 1215 2175
Size: 225 990 960
Fragments arranged by size:
990 960 225
PvuII CAG'CTG
Cuts at: 0 1735 2175
Size: 1735 440
SanDI GG'GwC_CC
Cuts at: 0 737 2175
Size: 737 1438
ScaI AGT'ACT
Cuts at: 0 1881 2175
Size: 1881 294
SfcI C'TryA_G
Cuts at: 0 131 221 998 1211 1505 1684 2175
Size: 131 90 777 213 294 179 491
Fragments arranged by size:
777 491 294 213 179 131 90
SmlI C'TyrA_G
Cuts at: 0 65 1256 1446 2175
Size: 65 1191 190 729
Fragments arranged by size:
1191 729 190 65
SspI AAT'ATT
Cuts at: 0 1521 1673 2175
Size: 1521 152 502
Fragments arranged by size:
1521 502 152
StyI C'CwwG_G
Cuts at: 0 1705 1892 2175
Size: 1705 187 283
Fragments arranged by size:
1705 283 187
TatI w'GTAC_w
Cuts at: 0 1104 1879 2175
Size: 1104 775 296
Fragments arranged by size:
1104 775 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1627 1659 2175
Size: 1627 32 516
Fragments arranged by size:
1627 516 32
XmnI GAAnn'nnTTC
Cuts at: 0 572 2175
Size: 572 1603
Enzymes that do cut:
Acc65I AcuI AleI ApaI ApoI AvaI BaeI BanI
BanII BbsI BciVI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstEII
BstXI Bsu36I BtgI DraIII DrdI EarI Eco57MI EcoO109I
FalI Hin4I HincII HindIII KpnI MmeI MslI MspA1I
NcoI NdeI NspI PflMI PpuMI PspOMI PstI PvuII
SanDI ScaI SfcI SmlI SspI StyI TatI TstI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BplI BsaAI BsaHI BsiEI
BsiWI BsmBI BspEI BsrBI BsrFI BssHII BstAPI BstBI
BstYI BstZ17I BtgZI BtsI ClaI CspCI DraI EaeI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI MfeI MluI MscI NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI ZraI