Restriction map for
pF1KB6188
(Linear) (Six-base) MAPSORT of: KIBB6188.seq Check: 7598 from: 1 to: 2109
KIBB6188.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1811 2109
Size: 1811 298
AclI AA'CG_TT
Cuts at: 0 449 2109
Size: 449 1660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 599 896 977 997 1244 2109
Size: 238 361 297 81 20 247 865
Fragments arranged by size:
865 361 297 247 238 81 20
AflIII A'CryG_T
Cuts at: 0 40 2109
Size: 40 2069
AleI CACnn'nnGTG
Cuts at: 0 1957 2109
Size: 1957 152
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 724 2109
Size: 724 1385
AlwNI CAG_nnn'CTG
Cuts at: 0 1056 2053 2109
Size: 1056 997 56
Fragments arranged by size:
1056 997 56
ApaI G_GGCC'C
Cuts at: 0 679 1381 1602 1905 2109
Size: 679 702 221 303 204
Fragments arranged by size:
702 679 303 221 204
AvaI C'yCGr_G
Cuts at: 0 1905 2109
Size: 1905 204
BanI G'GyrC_C
Cuts at: 0 387 1447 2109
Size: 387 1060 662
Fragments arranged by size:
1060 662 387
BanII G_rGCy'C
Cuts at: 0 221 679 1163 1381 1391 1602 1905 2109
Size: 221 458 484 218 10 211 303 204
Fragments arranged by size:
484 458 303 221 218 211 204 10
BbeI G_GCGC'C
Cuts at: 0 1451 2109
Size: 1451 658
BbsI GAAGACnn'nnnn_
Cuts at: 0 379 601 1197 1772 2109
Size: 379 222 596 575 337
Fragments arranged by size:
596 575 379 337 222
BbvCI CC'TCA_GC
Cuts at: 0 298 2109
Size: 298 1811
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1693 1727 2109
Size: 1693 34 382
Fragments arranged by size:
1693 382 34
BclI T'GATC_A
Cuts at: 0 995 2109
Size: 995 1114
BglI GCCn_nnn'nGGC
Cuts at: 0 879 1144 2065 2074 2109
Size: 879 265 921 9 35
Fragments arranged by size:
921 879 265 35 9
BglII A'GATC_T
Cuts at: 0 249 2109
Size: 249 1860
BlpI GC'TnA_GC
Cuts at: 0 19 64 2109
Size: 19 45 2045
Fragments arranged by size:
2045 45 19
Bme1580I G_kGCm'C
Cuts at: 0 679 1381 1602 1905 1988 2109
Size: 679 702 221 303 83 121
Fragments arranged by size:
702 679 303 221 121 83
BmgBI CAC'GTC
Cuts at: 0 192 2109
Size: 192 1917
BmrI ACTGGGnnnn_n'
Cuts at: 0 87 823 832 1409 2109
Size: 87 736 9 577 700
Fragments arranged by size:
736 700 577 87 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1078 1153 1345 1625 2109
Size: 540 538 75 192 280 484
Fragments arranged by size:
540 538 484 280 192 75
Bpu10I CC'TnA_GC
Cuts at: 0 298 2109
Size: 298 1811
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 2109
Size: 1147 962
BsaI GGTCTCn'nnnn_
Cuts at: 0 1463 1876 2109
Size: 1463 413 233
Fragments arranged by size:
1463 413 233
BsaBI GATnn'nnATC
Cuts at: 0 1930 1966 2109
Size: 1930 36 143
Fragments arranged by size:
1930 143 36
BsaHI Gr'CG_yC
Cuts at: 0 1448 2109
Size: 1448 661
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 166 196 386 416 566 596 1819 1849
Size: 166 30 190 30 150 30 1223 30
Cuts at: 1849 2062 2092 2109
Size: 213 30 17
Fragments arranged by size:
1223 213 190 166 150 30 30 30
30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 142 282 543 592 684 1265 1482 1933
Size: 142 140 261 49 92 581 217 451
Cuts at: 1933 2109
Size: 176
Fragments arranged by size:
581 451 261 217 176 142 140 92
49
BseYI C'CCAG_C
Cuts at: 0 639 787 1317 1391 1396 1553 1812 2109
Size: 639 148 530 74 5 157 259 297
Fragments arranged by size:
639 530 297 259 157 148 74 5
BsiHKAI G_wGCw'C
Cuts at: 0 365 1163 2109
Size: 365 798 946
Fragments arranged by size:
946 798 365
BsmI GAATG_Cn'
Cuts at: 0 1116 1738 2109
Size: 1116 622 371
Fragments arranged by size:
1116 622 371
BsmBI CGTCTCn'nnnn_
Cuts at: 0 322 2109
Size: 322 1787
Bsp1286I G_dGCh'C
Cuts at: 0 221 365 679 1163 1381 1391 1602 1905
Size: 221 144 314 484 218 10 211 303
Cuts at: 1905 1988 2109
Size: 83 121
Fragments arranged by size:
484 314 303 221 218 211 144 121
83 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 68 1811 2109
Size: 68 1743 298
Fragments arranged by size:
1743 298 68
BsrBI CCG'CTC
Cuts at: 0 928 2109
Size: 928 1181
BsrDI GCAATG_nn'
Cuts at: 0 1217 2031 2109
Size: 1217 814 78
Fragments arranged by size:
1217 814 78
BsrFI r'CCGG_y
Cuts at: 0 882 1502 2109
Size: 882 620 607
Fragments arranged by size:
882 620 607
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1669 2109
Size: 1669 440
BstYI r'GATC_y
Cuts at: 0 249 1586 2109
Size: 249 1337 523
Fragments arranged by size:
1337 523 249
Bsu36I CC'TnA_GG
Cuts at: 0 696 1382 1528 2109
Size: 696 686 146 581
Fragments arranged by size:
696 686 581 146
BtgI C'CryG_G
Cuts at: 0 223 898 1624 1868 1896 2109
Size: 223 675 726 244 28 213
Fragments arranged by size:
726 675 244 223 213 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 296 2109
Size: 296 1813
BtsI GCAGTG_nn'
Cuts at: 0 727 2109
Size: 727 1382
EaeI y'GGCC_r
Cuts at: 0 122 2109
Size: 122 1987
EarI CTCTTCn'nnn_
Cuts at: 0 148 291 907 1219 1489 2109
Size: 148 143 616 312 270 620
Fragments arranged by size:
620 616 312 270 148 143
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1090 2109
Size: 19 1071 1019
Fragments arranged by size:
1071 1019 19
EcoICRI GAG'CTC
Cuts at: 0 1161 2109
Size: 1161 948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 540 599 896 977 997 1078 1153
Size: 238 302 59 297 81 20 81 75
Cuts at: 1153 1244 1345 1625 2109
Size: 91 101 280 484
Fragments arranged by size:
484 302 297 280 238 101 91 81
81 75 59 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 850 2109
Size: 850 1259
EcoO109I rG'GnC_Cy
Cuts at: 0 582 1137 1367 1377 1378 1598 1901 1902
Size: 582 555 230 10 1 220 303 1
Cuts at: 1902 2109
Size: 207
Fragments arranged by size:
582 555 303 230 220 207 10 1
1
HaeII r_GCGC'y
Cuts at: 0 1451 1566 2109
Size: 1451 115 543
Fragments arranged by size:
1451 543 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 166 198 406 438 457 489 566 592
Size: 166 32 208 32 19 32 77 26
Cuts at: 592 598 624 679 711 1873 1905 2109
Size: 6 26 55 32 1162 32 204
Fragments arranged by size:
1162 208 204 166 77 55 32 32
32 32 32 26 26 19 6
HincII GTy'rAC
Cuts at: 0 522 2109
Size: 522 1587
KasI G'GCGC_C
Cuts at: 0 1447 2109
Size: 1447 662
MfeI C'AATT_G
Cuts at: 0 1751 2109
Size: 1751 358
MluI A'CGCG_T
Cuts at: 0 40 2109
Size: 40 2069
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1686 2109
Size: 1686 423
MslI CAynn'nnrTG
Cuts at: 0 1957 2109
Size: 1957 152
MspA1I CmG'CkG
Cuts at: 0 1701 2109
Size: 1701 408
NaeI GCC'GGC
Cuts at: 0 884 1504 2109
Size: 884 620 605
Fragments arranged by size:
884 620 605
NarI GG'CG_CC
Cuts at: 0 1448 2109
Size: 1448 661
NcoI C'CATG_G
Cuts at: 0 223 1868 2109
Size: 223 1645 241
Fragments arranged by size:
1645 241 223
NgoMIV G'CCGG_C
Cuts at: 0 882 1502 2109
Size: 882 620 607
Fragments arranged by size:
882 620 607
PasI CC'CwG_GG
Cuts at: 0 1374 1441 2109
Size: 1374 67 668
Fragments arranged by size:
1374 668 67
PfoI T'CCnGG_A
Cuts at: 0 584 1024 2109
Size: 584 440 1085
Fragments arranged by size:
1085 584 440
PpuMI rG'GwC_Cy
Cuts at: 0 582 1367 2109
Size: 582 785 742
Fragments arranged by size:
785 742 582
PshAI GACnn'nnGTC
Cuts at: 0 80 1614 2109
Size: 80 1534 495
Fragments arranged by size:
1534 495 80
PspOMI G'GGCC_C
Cuts at: 0 675 1377 1598 1901 2109
Size: 675 702 221 303 208
Fragments arranged by size:
702 675 303 221 208
PstI C_TGCA'G
Cuts at: 0 89 734 809 1052 2058 2109
Size: 89 645 75 243 1006 51
Fragments arranged by size:
1006 645 243 89 75 51
PvuII CAG'CTG
Cuts at: 0 1701 2109
Size: 1701 408
SacI G_AGCT'C
Cuts at: 0 1163 2109
Size: 1163 946
SapI GCTCTTCn'nnn_
Cuts at: 0 148 291 907 1489 2109
Size: 148 143 616 582 620
Fragments arranged by size:
620 616 582 148 143
ScaI AGT'ACT
Cuts at: 0 1079 2109
Size: 1079 1030
SfcI C'TryA_G
Cuts at: 0 85 730 743 805 1048 2054 2109
Size: 85 645 13 62 243 1006 55
Fragments arranged by size:
1006 645 243 85 62 55 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2074 2109
Size: 2074 35
SfoI GGC'GCC
Cuts at: 0 1449 2109
Size: 1449 660
SmaI CCC'GGG
Cuts at: 0 1907 2109
Size: 1907 202
SmlI C'TyrA_G
Cuts at: 0 1162 2109
Size: 1162 947
StuI AGG'CCT
Cuts at: 0 532 2077 2109
Size: 532 1545 32
Fragments arranged by size:
1545 532 32
StyI C'CwwG_G
Cuts at: 0 223 383 1451 1868 2109
Size: 223 160 1068 417 241
Fragments arranged by size:
1068 417 241 223 160
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1733 1772 2109
Size: 1733 39 337
Fragments arranged by size:
1733 337 39
TatI w'GTAC_w
Cuts at: 0 1077 2109
Size: 1077 1032
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 386 418 2062 2094 2109
Size: 386 32 1644 32 15
Fragments arranged by size:
1644 386 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 0 378 600 1887 2109
Size: 378 222 1287 222
Fragments arranged by size:
1287 378 222 222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1851 2109
Size: 1851 258
XmaI C'CCGG_G
Cuts at: 0 1905 2109
Size: 1905 204
Enzymes that do cut:
AarI AclI AcuI AflIII AleI AlfI AlwNI ApaI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstXI
BstYI Bsu36I BtgI BtgZI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
MfeI MluI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV PasI PfoI PpuMI PshAI PspOMI PstI PvuII
SacI SapI ScaI SfcI SfiI SfoI SmaI SmlI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BfrBI BmtI BplI BsaAI BsaWI BsgI BsiEI
BsiWI BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MscI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI XmnI ZraI