Restriction map for
pF1KB6183
(Linear) (Six-base) MAPSORT of: KIBB6183.seq Check: 5967 from: 1 to: 1977
KIBB6183.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 299 1752 1977
Size: 299 1453 225
Fragments arranged by size:
1453 299 225
AatII G_ACGT'C
Cuts at: 0 553 1977
Size: 553 1424
AccI GT'mk_AC
Cuts at: 0 90 1977
Size: 90 1887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1493 1810 1977
Size: 525 968 317 167
Fragments arranged by size:
968 525 317 167
AfeI AGC'GCT
Cuts at: 0 1114 1977
Size: 1114 863
AhdI GACnn_n'nnGTC
Cuts at: 0 756 1977
Size: 756 1221
AleI CACnn'nnGTG
Cuts at: 0 52 514 1222 1977
Size: 52 462 708 755
Fragments arranged by size:
755 708 462 52
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 128 1240 1977
Size: 128 1112 737
Fragments arranged by size:
1112 737 128
AlwNI CAG_nnn'CTG
Cuts at: 0 291 312 897 957 1164 1317 1471 1510
Size: 291 21 585 60 207 153 154 39
Cuts at: 1510 1977
Size: 467
Fragments arranged by size:
585 467 291 207 154 153 60 39
21
ApaI G_GGCC'C
Cuts at: 0 62 1977
Size: 62 1915
AvaI C'yCGr_G
Cuts at: 0 30 204 1085 1977
Size: 30 174 881 892
Fragments arranged by size:
892 881 174 30
BamHI G'GATC_C
Cuts at: 0 250 1977
Size: 250 1727
BanI G'GyrC_C
Cuts at: 0 40 354 1216 1977
Size: 40 314 862 761
Fragments arranged by size:
862 761 314 40
BanII G_rGCy'C
Cuts at: 0 62 221 917 1018 1337 1637 1898 1977
Size: 62 159 696 101 319 300 261 79
Fragments arranged by size:
696 319 300 261 159 101 79 62
BbeI G_GCGC'C
Cuts at: 0 44 1977
Size: 44 1933
BbsI GAAGACnn'nnnn_
Cuts at: 0 1618 1677 1694 1977
Size: 1618 59 17 283
Fragments arranged by size:
1618 283 59 17
BbvCI CC'TCA_GC
Cuts at: 0 313 1778 1977
Size: 313 1465 199
Fragments arranged by size:
1465 313 199
BglI GCCn_nnn'nGGC
Cuts at: 0 57 369 1834 1977
Size: 57 312 1465 143
Fragments arranged by size:
1465 312 143 57
BglII A'GATC_T
Cuts at: 0 1382 1977
Size: 1382 595
BlpI GC'TnA_GC
Cuts at: 0 830 1257 1977
Size: 830 427 720
Fragments arranged by size:
830 720 427
Bme1580I G_kGCm'C
Cuts at: 0 62 675 1219 1977
Size: 62 613 544 758
Fragments arranged by size:
758 613 544 62
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1674 1704 1977
Size: 1674 30 273
Fragments arranged by size:
1674 273 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 370 522 595 775 883 907 919
Size: 79 291 152 73 180 108 24 12
Cuts at: 919 1060 1327 1651 1669 1801 1977
Size: 141 267 324 18 132 176
Fragments arranged by size:
324 291 267 180 176 152 141 132
108 79 73 24 18 12
Bpu10I CC'TnA_GC
Cuts at: 0 313 1109 1778 1977
Size: 313 796 669 199
Fragments arranged by size:
796 669 313 199
BsaI GGTCTCn'nnnn_
Cuts at: 0 1452 1657 1977
Size: 1452 205 320
Fragments arranged by size:
1452 320 205
BsaAI yAC'GTr
Cuts at: 0 1224 1326 1591 1977
Size: 1224 102 265 386
Fragments arranged by size:
1224 386 265 102
BsaHI Gr'CG_yC
Cuts at: 0 41 550 1247 1977
Size: 41 509 697 730
Fragments arranged by size:
730 697 509 41
BsaWI w'CCGG_w
Cuts at: 0 1841 1922 1977
Size: 1841 81 55
Fragments arranged by size:
1841 81 55
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 448 478 970 1000 1977
Size: 448 30 492 30 977
Fragments arranged by size:
977 492 448 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 229 289 1057 1162 1471 1540 1977
Size: 226 3 60 768 105 309 69 437
Fragments arranged by size:
768 437 309 226 105 69 60 3
BseYI C'CCAG_C
Cuts at: 0 344 469 772 1157 1464 1977
Size: 344 125 303 385 307 513
Fragments arranged by size:
513 385 344 307 303 125
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 459 1348 1977
Size: 277 182 889 629
Fragments arranged by size:
889 629 277 182
BsiHKAI G_wGCw'C
Cuts at: 0 122 221 1212 1337 1463 1657 1977
Size: 122 99 991 125 126 194 320
Fragments arranged by size:
991 320 194 126 125 122 99
BsmI GAATG_Cn'
Cuts at: 0 1303 1977
Size: 1303 674
BsmBI CGTCTCn'nnnn_
Cuts at: 0 421 524 1174 1977
Size: 421 103 650 803
Fragments arranged by size:
803 650 421 103
Bsp1286I G_dGCh'C
Cuts at: 0 62 122 221 675 917 1018 1212 1219
Size: 62 60 99 454 242 101 194 7
Cuts at: 1219 1337 1463 1637 1657 1898 1977
Size: 118 126 174 20 241 79
Fragments arranged by size:
454 242 241 194 174 126 118 101
99 79 62 60 20 7
BspEI T'CCGG_A
Cuts at: 0 1922 1977
Size: 1922 55
BspHI T'CATG_A
Cuts at: 0 1610 1977
Size: 1610 367
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 299 389 1752 1977
Size: 299 90 1363 225
Fragments arranged by size:
1363 299 225 90
BsrBI CCG'CTC
Cuts at: 0 1256 1977
Size: 1256 721
BssSI C'ACGA_G
Cuts at: 0 398 1696 1977
Size: 398 1298 281
Fragments arranged by size:
1298 398 281
BstAPI GCAn_nnn'nTGC
Cuts at: 0 291 1317 1977
Size: 291 1026 660
Fragments arranged by size:
1026 660 291
BstEII G'GTnAC_C
Cuts at: 0 1062 1809 1977
Size: 1062 747 168
Fragments arranged by size:
1062 747 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 1272 1977
Size: 54 1218 705
Fragments arranged by size:
1218 705 54
BstYI r'GATC_y
Cuts at: 0 250 569 884 1382 1977
Size: 250 319 315 498 595
Fragments arranged by size:
595 498 319 315 250
Bsu36I CC'TnA_GG
Cuts at: 0 1511 1977
Size: 1511 466
BtgI C'CryG_G
Cuts at: 0 53 123 222 1582 1829 1977
Size: 53 70 99 1360 247 148
Fragments arranged by size:
1360 247 148 99 70 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1329 1977
Size: 1329 648
BtsI GCAGTG_nn'
Cuts at: 0 675 1233 1830 1977
Size: 675 558 597 147
Fragments arranged by size:
675 597 558 147
DraIII CAC_nnn'GTG
Cuts at: 0 1131 1977
Size: 1131 846
DrdI GACnn_nn'nnGTC
Cuts at: 0 73 1977
Size: 73 1904
EaeI y'GGCC_r
Cuts at: 0 746 1262 1403 1585 1835 1977
Size: 746 516 141 182 250 142
Fragments arranged by size:
746 516 250 182 142 141
EarI CTCTTCn'nnn_
Cuts at: 0 1502 1541 1565 1695 1706 1977
Size: 1502 39 24 130 11 271
Fragments arranged by size:
1502 271 130 39 24 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 322 877 1490 1977
Size: 322 555 613 487
Fragments arranged by size:
613 555 487 322
EcoICRI GAG'CTC
Cuts at: 0 219 1335 1977
Size: 219 1116 642
Fragments arranged by size:
1116 642 219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 370 522 525 595 775 883 907
Size: 79 291 152 3 70 180 108 24
Cuts at: 907 919 1060 1327 1493 1651 1669 1801 1810
Size: 12 141 267 166 158 18 132 9
Cuts at: 1810 1977
Size: 167
Fragments arranged by size:
291 267 180 167 166 158 152 141
132 108 79 70 24 18 12 9
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1309 1977
Size: 1309 668
EcoO109I rG'GnC_Cy
Cuts at: 0 58 59 201 791 1314 1774 1977
Size: 58 1 142 590 523 460 203
Fragments arranged by size:
590 523 460 203 142 58 1
HaeII r_GCGC'y
Cuts at: 0 44 779 1116 1977
Size: 44 735 337 861
Fragments arranged by size:
861 735 337 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 742 774 970 1002 1135 1167
Size: 448 32 262 32 196 32 133 32
Cuts at: 1167 1977
Size: 810
Fragments arranged by size:
810 448 262 196 133 32 32 32
32
HincII GTy'rAC
Cuts at: 0 91 1977
Size: 91 1886
KasI G'GCGC_C
Cuts at: 0 40 1977
Size: 40 1937
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1977
Size: 827 1150
MscI TGG'CCA
Cuts at: 0 1837 1977
Size: 1837 140
MslI CAynn'nnrTG
Cuts at: 0 52 514 1222 1955 1977
Size: 52 462 708 733 22
Fragments arranged by size:
733 708 462 52 22
MspA1I CmG'CkG
Cuts at: 0 38 309 348 473 650 975 1161 1261
Size: 38 271 39 125 177 325 186 100
Cuts at: 1261 1377 1977
Size: 116 600
Fragments arranged by size:
600 325 271 186 177 125 116 100
39 38
NarI GG'CG_CC
Cuts at: 0 41 1977
Size: 41 1936
NspI r_CATG'y
Cuts at: 0 632 1677 1977
Size: 632 1045 300
Fragments arranged by size:
1045 632 300
PasI CC'CwG_GG
Cuts at: 0 62 480 1977
Size: 62 418 1497
Fragments arranged by size:
1497 418 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 897 1227 1764 1806 1977
Size: 897 330 537 42 171
Fragments arranged by size:
897 537 330 171 42
PfoI T'CCnGG_A
Cuts at: 0 1856 1977
Size: 1856 121
PmlI CAC'GTG
Cuts at: 0 1224 1326 1977
Size: 1224 102 651
Fragments arranged by size:
1224 651 102
PpuMI rG'GwC_Cy
Cuts at: 0 791 1977
Size: 791 1186
PspOMI G'GGCC_C
Cuts at: 0 58 1977
Size: 58 1919
PstI C_TGCA'G
Cuts at: 0 146 1052 1382 1724 1977
Size: 146 906 330 342 253
Fragments arranged by size:
906 342 330 253 146
PvuII CAG'CTG
Cuts at: 0 309 348 473 1161 1377 1977
Size: 309 39 125 688 216 600
Fragments arranged by size:
688 600 309 216 125 39
SacI G_AGCT'C
Cuts at: 0 221 1337 1977
Size: 221 1116 640
Fragments arranged by size:
1116 640 221
SalI G'TCGA_C
Cuts at: 0 89 1977
Size: 89 1888
SapI GCTCTTCn'nnn_
Cuts at: 0 1565 1706 1977
Size: 1565 141 271
Fragments arranged by size:
1565 271 141
SfcI C'TryA_G
Cuts at: 0 142 1048 1378 1720 1977
Size: 142 906 330 342 257
Fragments arranged by size:
906 342 330 257 142
SfoI GGC'GCC
Cuts at: 0 42 1977
Size: 42 1935
SmaI CCC'GGG
Cuts at: 0 32 1087 1977
Size: 32 1055 890
Fragments arranged by size:
1055 890 32
StuI AGG'CCT
Cuts at: 0 1852 1977
Size: 1852 125
StyI C'CwwG_G
Cuts at: 0 1640 1748 1977
Size: 1640 108 229
Fragments arranged by size:
1640 229 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 526 995 1081 1234 1429 1478 1977
Size: 526 469 86 153 195 49 499
Fragments arranged by size:
526 499 469 195 153 86 49
TatI w'GTAC_w
Cuts at: 0 518 1977
Size: 518 1459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 477 1626 1977
Size: 477 1149 351
Fragments arranged by size:
1149 477 351
XmaI C'CCGG_G
Cuts at: 0 30 1085 1977
Size: 30 1055 892
Fragments arranged by size:
1055 892 30
ZraI GAC'GTC
Cuts at: 0 551 1977
Size: 551 1426
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AleI AlfI
AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SalI SapI SfcI
SfoI SmaI StuI StyI TaqII TatI XcmI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI
BfrBI BmgBI BmrI BmtI BpuEI BsaBI BsiEI BsiWI
BsrDI BsrFI BsrGI BssHII BstBI BstZ17I ClaI CspCI
DraI EagI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XhoI XmnI