Restriction map for
pF1KB6175
(Linear) (Six-base) MAPSORT of: KIBB6175.seq Check: 5286 from: 1 to: 1959
KIBB6175.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 500 1253 1959
Size: 500 753 706
Fragments arranged by size:
753 706 500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 426 643 727 1490 1959
Size: 426 217 84 763 469
Fragments arranged by size:
763 469 426 217 84
AfeI AGC'GCT
Cuts at: 0 535 976 1959
Size: 535 441 983
Fragments arranged by size:
983 535 441
AflIII A'CryG_T
Cuts at: 0 1769 1959
Size: 1769 190
AleI CACnn'nnGTG
Cuts at: 0 604 1772 1801 1959
Size: 604 1168 29 158
Fragments arranged by size:
1168 604 158 29
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 622 1740 1959
Size: 622 1118 219
Fragments arranged by size:
1118 622 219
AlwNI CAG_nnn'CTG
Cuts at: 0 492 804 1706 1785 1959
Size: 492 312 902 79 174
Fragments arranged by size:
902 492 312 174 79
ApaI G_GGCC'C
Cuts at: 0 513 1342 1590 1795 1855 1959
Size: 513 829 248 205 60 104
Fragments arranged by size:
829 513 248 205 104 60
ApaLI G'TGCA_C
Cuts at: 0 419 426 749 1959
Size: 419 7 323 1210
Fragments arranged by size:
1210 419 323 7
AvaI C'yCGr_G
Cuts at: 0 42 125 931 1085 1959
Size: 42 83 806 154 874
Fragments arranged by size:
874 806 154 83 42
BamHI G'GATC_C
Cuts at: 0 46 1959
Size: 46 1913
BanI G'GyrC_C
Cuts at: 0 1119 1265 1818 1959
Size: 1119 146 553 141
Fragments arranged by size:
1119 553 146 141
BanII G_rGCy'C
Cuts at: 0 513 740 1292 1342 1556 1590 1795 1855
Size: 513 227 552 50 214 34 205 60
Cuts at: 1855 1959
Size: 104
Fragments arranged by size:
552 513 227 214 205 104 60 50
34
BbsI GAAGACnn'nnnn_
Cuts at: 0 214 1240 1959
Size: 214 1026 719
Fragments arranged by size:
1026 719 214
BbvCI CC'TCA_GC
Cuts at: 0 177 940 1394 1959
Size: 177 763 454 565
Fragments arranged by size:
763 565 454 177
BglI GCCn_nnn'nGGC
Cuts at: 0 508 1790 1807 1934 1959
Size: 508 1282 17 127 25
Fragments arranged by size:
1282 508 127 25 17
BlpI GC'TnA_GC
Cuts at: 0 520 1959
Size: 520 1439
Bme1580I G_kGCm'C
Cuts at: 0 423 430 513 753 1342 1590 1795 1855
Size: 423 7 83 240 589 248 205 60
Cuts at: 1855 1955 1959
Size: 100 4
Fragments arranged by size:
589 423 248 240 205 100 83 60
7 4
BmgBI CAC'GTC
Cuts at: 0 1404 1697 1959
Size: 1404 293 262
Fragments arranged by size:
1404 293 262
BmrI ACTGGGnnnn_n'
Cuts at: 0 148 771 1308 1761 1959
Size: 148 623 537 453 198
Fragments arranged by size:
623 537 453 198 148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 1051 1479 1638 1959
Size: 106 945 428 159 321
Fragments arranged by size:
945 428 321 159 106
Bpu10I CC'TnA_GC
Cuts at: 0 170 177 940 1394 1959
Size: 170 7 763 454 565
Fragments arranged by size:
763 565 454 170 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 1959
Size: 514 1445
BsaAI yAC'GTr
Cuts at: 0 1443 1770 1959
Size: 1443 327 189
Fragments arranged by size:
1443 327 189
BsaHI Gr'CG_yC
Cuts at: 0 769 812 883 1319 1622 1959
Size: 769 43 71 436 303 337
Fragments arranged by size:
769 436 337 303 71 43
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 655 813 828 891 1300 1959
Size: 655 158 15 63 409 659
Fragments arranged by size:
659 655 409 158 63 15
BseYI C'CCAG_C
Cuts at: 0 147 195 232 498 609 788 971 985
Size: 147 48 37 266 111 179 183 14
Cuts at: 985 1882 1924 1959
Size: 897 42 35
Fragments arranged by size:
897 266 183 179 147 111 48 42
37 35 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 910 1719 1959
Size: 727 183 809 240
Fragments arranged by size:
809 727 240 183
BsiEI CG_ry'CG
Cuts at: 0 339 638 688 1111 1281 1959
Size: 339 299 50 423 170 678
Fragments arranged by size:
678 423 339 299 170 50
BsiHKAI G_wGCw'C
Cuts at: 0 423 430 740 753 938 1292 1556 1959
Size: 423 7 310 13 185 354 264 403
Fragments arranged by size:
423 403 354 310 264 185 13 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1687 1959
Size: 1687 272
Bsp1286I G_dGCh'C
Cuts at: 0 423 430 513 740 753 938 1292 1342
Size: 423 7 83 227 13 185 354 50
Cuts at: 1342 1556 1590 1795 1855 1955 1959
Size: 214 34 205 60 100 4
Fragments arranged by size:
423 354 227 214 205 185 100 83
60 50 34 13 7 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 500 725 1253 1959
Size: 500 225 528 706
Fragments arranged by size:
706 528 500 225
BsrBI CCG'CTC
Cuts at: 0 92 1345 1584 1959
Size: 92 1253 239 375
Fragments arranged by size:
1253 375 239 92
BsrDI GCAATG_nn'
Cuts at: 0 453 1959
Size: 453 1506
BsrFI r'CCGG_y
Cuts at: 0 3 109 1378 1435 1891 1959
Size: 3 106 1269 57 456 68
Fragments arranged by size:
1269 456 106 68 57 3
BssSI C'ACGA_G
Cuts at: 0 1549 1959
Size: 1549 410
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 804 1706 1959
Size: 81 723 902 253
Fragments arranged by size:
902 723 253 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1774 1803 1959
Size: 1774 29 156
Fragments arranged by size:
1774 156 29
BstYI r'GATC_y
Cuts at: 0 46 1959
Size: 46 1913
Bsu36I CC'TnA_GG
Cuts at: 0 1448 1959
Size: 1448 511
BtgI C'CryG_G
Cuts at: 0 333 1043 1352 1562 1802 1959
Size: 333 710 309 210 240 157
Fragments arranged by size:
710 333 309 240 210 157
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 196 1959
Size: 196 1763
BtsI GCAGTG_nn'
Cuts at: 0 547 1959
Size: 547 1412
DraIII CAC_nnn'GTG
Cuts at: 0 81 1770 1959
Size: 81 1689 189
Fragments arranged by size:
1689 189 81
EaeI y'GGCC_r
Cuts at: 0 336 455 685 758 949 1108 1592 1776
Size: 336 119 230 73 191 159 484 184
Cuts at: 1776 1808 1959
Size: 32 151
Fragments arranged by size:
484 336 230 191 184 159 151 119
73 32
EagI C'GGCC_G
Cuts at: 0 336 685 1108 1959
Size: 336 349 423 851
Fragments arranged by size:
851 423 349 336
EarI CTCTTCn'nnn_
Cuts at: 0 1175 1466 1959
Size: 1175 291 493
Fragments arranged by size:
1175 493 291
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1365 1695 1959
Size: 1365 330 264
Fragments arranged by size:
1365 330 264
EcoICRI GAG'CTC
Cuts at: 0 738 1290 1554 1959
Size: 738 552 264 405
Fragments arranged by size:
738 552 405 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 426 643 727 1051 1479 1490 1638
Size: 106 320 217 84 324 428 11 148
Cuts at: 1638 1959
Size: 321
Fragments arranged by size:
428 324 321 320 217 148 106 84
11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1594 1959
Size: 1594 365
EcoO109I rG'GnC_Cy
Cuts at: 0 130 509 1127 1338 1339 1390 1427 1587
Size: 130 379 618 211 1 51 37 160
Cuts at: 1587 1730 1791 1792 1852 1903 1959
Size: 143 61 1 60 51 56
Fragments arranged by size:
618 379 211 160 143 130 61 60
56 51 51 37 1 1
FspI TGC'GCA
Cuts at: 0 598 1261 1959
Size: 598 663 698
Fragments arranged by size:
698 663 598
FspAI rTGC'GCAy
Cuts at: 0 598 1959
Size: 598 1361
HaeII r_GCGC'y
Cuts at: 0 537 821 978 1104 1245 1311 1959
Size: 537 284 157 126 141 66 648
Fragments arranged by size:
648 537 284 157 141 126 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 892 924 1959
Size: 892 32 1035
Fragments arranged by size:
1035 892 32
HincII GTy'rAC
Cuts at: 0 861 1959
Size: 861 1098
HindIII A'AGCT_T
Cuts at: 0 655 1959
Size: 655 1304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1487 1508 1959
Size: 1487 21 451
Fragments arranged by size:
1487 451 21
MscI TGG'CCA
Cuts at: 0 760 1778 1959
Size: 760 1018 181
Fragments arranged by size:
1018 760 181
MslI CAynn'nnrTG
Cuts at: 0 604 1042 1222 1772 1801 1959
Size: 604 438 180 550 29 158
Fragments arranged by size:
604 550 438 180 158 29
MspA1I CmG'CkG
Cuts at: 0 199 732 924 1354 1703 1959
Size: 199 533 192 430 349 256
Fragments arranged by size:
533 430 349 256 199 192
NaeI GCC'GGC
Cuts at: 0 5 111 1893 1959
Size: 5 106 1782 66
Fragments arranged by size:
1782 106 66 5
NcoI C'CATG_G
Cuts at: 0 1043 1959
Size: 1043 916
NgoMIV G'CCGG_C
Cuts at: 0 3 109 1891 1959
Size: 3 106 1782 68
Fragments arranged by size:
1782 106 68 3
NruI TCG'CGA
Cuts at: 0 1412 1959
Size: 1412 547
NspI r_CATG'y
Cuts at: 0 240 1959
Size: 240 1719
PasI CC'CwG_GG
Cuts at: 0 264 1959
Size: 264 1695
PflMI CCAn_nnn'nTGG
Cuts at: 0 583 783 1848 1959
Size: 583 200 1065 111
Fragments arranged by size:
1065 583 200 111
PmlI CAC'GTG
Cuts at: 0 1770 1959
Size: 1770 189
PpuMI rG'GwC_Cy
Cuts at: 0 130 1127 1427 1730 1959
Size: 130 997 300 303 229
Fragments arranged by size:
997 303 300 229 130
PspOMI G'GGCC_C
Cuts at: 0 509 1338 1586 1791 1851 1959
Size: 509 829 248 205 60 108
Fragments arranged by size:
829 509 248 205 108 60
PstI C_TGCA'G
Cuts at: 0 554 731 1517 1790 1959
Size: 554 177 786 273 169
Fragments arranged by size:
786 554 273 177 169
PvuI CG_AT'CG
Cuts at: 0 638 1959
Size: 638 1321
SacI G_AGCT'C
Cuts at: 0 740 1292 1556 1959
Size: 740 552 264 403
Fragments arranged by size:
740 552 403 264
SacII CC_GC'GG
Cuts at: 0 1355 1959
Size: 1355 604
SanDI GG'GwC_CC
Cuts at: 0 130 1127 1959
Size: 130 997 832
Fragments arranged by size:
997 832 130
SbfI CC_TGCA'GG
Cuts at: 0 1790 1959
Size: 1790 169
ScaI AGT'ACT
Cuts at: 0 781 1959
Size: 781 1178
SfcI C'TryA_G
Cuts at: 0 550 727 978 1311 1513 1786 1959
Size: 550 177 251 333 202 273 173
Fragments arranged by size:
550 333 273 251 202 177 173
SmaI CCC'GGG
Cuts at: 0 127 1087 1959
Size: 127 960 872
Fragments arranged by size:
960 872 127
SmlI C'TyrA_G
Cuts at: 0 529 1959
Size: 529 1430
SphI G_CATG'C
Cuts at: 0 240 1959
Size: 240 1719
StuI AGG'CCT
Cuts at: 0 175 1612 1959
Size: 175 1437 347
Fragments arranged by size:
1437 347 175
StyI C'CwwG_G
Cuts at: 0 1043 1959
Size: 1043 916
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 447 1091 1858 1959
Size: 447 644 767 101
Fragments arranged by size:
767 644 447 101
TatI w'GTAC_w
Cuts at: 0 779 1959
Size: 779 1180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1674 1706 1959
Size: 1674 32 253
Fragments arranged by size:
1674 253 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1803 1959
Size: 1803 156
XmaI C'CCGG_G
Cuts at: 0 125 1085 1959
Size: 125 960 874
Fragments arranged by size:
960 874 125
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FspI FspAI HaeII
Hin4I HincII HindIII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NruI NspI PasI PflMI PmlI PpuMI
PspOMI PstI PvuI SacI SacII SanDI SbfI ScaI
SfcI SmaI SmlI SphI StuI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI
BciVI BclI BfrBI BglII BmtI BplI BsaI BsaBI
BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrGI BssHII
BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EcoRI
EcoRV FalI FseI HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NsiI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuII RsrII
SalI SapI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI
ZraI