Restriction map for
pF1KB6153
(Linear) (Six-base) MAPSORT of: KIBB6153.seq Check: 7017 from: 1 to: 1791
KIBB6153.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1221 1791
Size: 1221 570
AccI GT'mk_AC
Cuts at: 0 1683 1791
Size: 1683 108
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 455 659 772 1111 1243 1447 1791
Size: 455 204 113 339 132 204 344
Fragments arranged by size:
455 344 339 204 204 132 113
AflII C'TTAA_G
Cuts at: 0 649 1791
Size: 649 1142
AflIII A'CryG_T
Cuts at: 0 716 1791
Size: 716 1075
AlwNI CAG_nnn'CTG
Cuts at: 0 1180 1236 1633 1791
Size: 1180 56 397 158
Fragments arranged by size:
1180 397 158 56
ApaLI G'TGCA_C
Cuts at: 0 1288 1791
Size: 1288 503
ApoI r'AATT_y
Cuts at: 0 255 595 681 709 1791
Size: 255 340 86 28 1082
Fragments arranged by size:
1082 340 255 86 28
AseI AT'TA_AT
Cuts at: 0 185 1791
Size: 185 1606
BamHI G'GATC_C
Cuts at: 0 1621 1791
Size: 1621 170
BanI G'GyrC_C
Cuts at: 0 1348 1408 1791
Size: 1348 60 383
Fragments arranged by size:
1348 383 60
BanII G_rGCy'C
Cuts at: 0 1255 1791
Size: 1255 536
BbsI GAAGACnn'nnnn_
Cuts at: 0 161 461 958 1370 1791
Size: 161 300 497 412 421
Fragments arranged by size:
497 421 412 300 161
BbvCI CC'TCA_GC
Cuts at: 0 297 1791
Size: 297 1494
BfrBI ATG'CAT
Cuts at: 0 564 1773 1791
Size: 564 1209 18
Fragments arranged by size:
1209 564 18
BglI GCCn_nnn'nGGC
Cuts at: 0 400 1791
Size: 400 1391
BglII A'GATC_T
Cuts at: 0 759 1703 1791
Size: 759 944 88
Fragments arranged by size:
944 759 88
Bme1580I G_kGCm'C
Cuts at: 0 526 1292 1791
Size: 526 766 499
Fragments arranged by size:
766 526 499
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1178 1791
Size: 1178 613
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1791
Size: 76 1715
Bpu10I CC'TnA_GC
Cuts at: 0 297 1791
Size: 297 1494
BsaAI yAC'GTr
Cuts at: 0 717 1791
Size: 717 1074
BsaBI GATnn'nnATC
Cuts at: 0 1634 1791
Size: 1634 157
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 907 937 1332 1362 1791
Size: 907 30 395 30 429
Fragments arranged by size:
907 429 395 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 931 1791
Size: 931 860
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 725 1791
Size: 725 1066
BsiEI CG_ry'CG
Cuts at: 0 909 1791
Size: 909 882
BsiHKAI G_wGCw'C
Cuts at: 0 1292 1791
Size: 1292 499
BsmI GAATG_Cn'
Cuts at: 0 1111 1791
Size: 1111 680
Bsp1286I G_dGCh'C
Cuts at: 0 526 1255 1292 1791
Size: 526 729 37 499
Fragments arranged by size:
729 526 499 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1221 1791
Size: 1221 570
BsrGI T'GTAC_A
Cuts at: 0 264 1791
Size: 264 1527
BstXI CCAn_nnnn'nTGG
Cuts at: 0 428 897 1678 1791
Size: 428 469 781 113
Fragments arranged by size:
781 469 428 113
BstYI r'GATC_y
Cuts at: 0 759 1621 1703 1791
Size: 759 862 82 88
Fragments arranged by size:
862 759 88 82
Bsu36I CC'TnA_GG
Cuts at: 0 1153 1791
Size: 1153 638
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1732 1791
Size: 1732 59
BtsI GCAGTG_nn'
Cuts at: 0 502 721 902 953 1791
Size: 502 219 181 51 838
Fragments arranged by size:
838 502 219 181 51
ClaI AT'CG_AT
Cuts at: 0 462 1791
Size: 462 1329
DraI TTT'AAA
Cuts at: 0 1491 1791
Size: 1491 300
DrdI GACnn_nn'nnGTC
Cuts at: 0 74 1791
Size: 74 1717
EaeI y'GGCC_r
Cuts at: 0 906 1782 1791
Size: 906 876 9
Fragments arranged by size:
906 876 9
EagI C'GGCC_G
Cuts at: 0 906 1791
Size: 906 885
EarI CTCTTCn'nnn_
Cuts at: 0 860 935 1039 1791
Size: 860 75 104 752
Fragments arranged by size:
860 752 104 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 455 659 772 1111 1243 1447 1791
Size: 76 379 204 113 339 132 204 344
Fragments arranged by size:
379 344 339 204 204 132 113 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 148 1791
Size: 148 1643
EcoRI G'AATT_C
Cuts at: 0 255 1791
Size: 255 1536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 174 664 1791
Size: 174 490 1127
Fragments arranged by size:
1127 490 174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 451 483 838 870 1549 1581 1606 1638
Size: 451 32 355 32 679 32 25 32
Cuts at: 1638 1791
Size: 153
Fragments arranged by size:
679 451 355 153 32 32 32 32
25
HincII GTy'rAC
Cuts at: 0 1524 1791
Size: 1524 267
HindIII A'AGCT_T
Cuts at: 0 1093 1791
Size: 1093 698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1031 1791
Size: 437 594 760
Fragments arranged by size:
760 594 437
MscI TGG'CCA
Cuts at: 0 1784 1791
Size: 1784 7
MslI CAynn'nnrTG
Cuts at: 0 803 1264 1791
Size: 803 461 527
Fragments arranged by size:
803 527 461
MspA1I CmG'CkG
Cuts at: 0 54 1221 1791
Size: 54 1167 570
Fragments arranged by size:
1167 570 54
NsiI A_TGCA'T
Cuts at: 0 566 1775 1791
Size: 566 1209 16
Fragments arranged by size:
1209 566 16
NspI r_CATG'y
Cuts at: 0 568 1768 1791
Size: 568 1200 23
Fragments arranged by size:
1200 568 23
PfoI T'CCnGG_A
Cuts at: 0 38 1791
Size: 38 1753
PmlI CAC'GTG
Cuts at: 0 717 1791
Size: 717 1074
PsiI TTA'TAA
Cuts at: 0 536 1145 1791
Size: 536 609 646
Fragments arranged by size:
646 609 536
PstI C_TGCA'G
Cuts at: 0 1232 1791
Size: 1232 559
PvuII CAG'CTG
Cuts at: 0 54 1221 1791
Size: 54 1167 570
Fragments arranged by size:
1167 570 54
SapI GCTCTTCn'nnn_
Cuts at: 0 860 1791
Size: 860 931
SfcI C'TryA_G
Cuts at: 0 1228 1791
Size: 1228 563
SmlI C'TyrA_G
Cuts at: 0 649 1791
Size: 649 1142
SphI G_CATG'C
Cuts at: 0 568 1791
Size: 568 1223
SspI AAT'ATT
Cuts at: 0 1594 1791
Size: 1594 197
StyI C'CwwG_G
Cuts at: 0 1677 1791
Size: 1677 114
TatI w'GTAC_w
Cuts at: 0 122 264 1791
Size: 122 142 1527
Fragments arranged by size:
1527 142 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 792 824 907 939 1332 1364 1791
Size: 792 32 83 32 393 32 427
Fragments arranged by size:
792 427 393 83 32 32 32
XbaI T'CTAG_A
Cuts at: 0 117 1791
Size: 117 1674
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 450 615 1791
Size: 450 165 1176
Fragments arranged by size:
1176 450 165
Enzymes that do cut:
AarI AccI AcuI AflII AflIII AlwNI ApaLI ApoI
AseI BamHI BanI BanII BbsI BbvCI BfrBI BglI
BglII Bme1580I BplI BpmI Bpu10I BsaAI BsaBI BsaXI
BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrGI
BstXI BstYI Bsu36I BtgZI BtsI ClaI DraI DrdI
EaeI EagI EarI Eco57MI EcoNI EcoRI FalI Hin4I
HincII HindIII MmeI MscI MslI MspA1I NsiI NspI
PfoI PmlI PsiI PstI PvuII SapI SfcI SmlI
SphI SspI StyI TatI TstI XbaI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AgeI AhdI AleI AlfI
AloI ApaI AscI AsiSI AvaI AvrII BaeI BbeI
BcgI BciVI BclI BlpI BmgBI BmrI BmtI BpuEI
BsaI BsaHI BsaWI BseYI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI CspCI DraIII EciI EcoICRI EcoO109I EcoRV
FseI FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII
Tth111I XhoI XmaI XmnI ZraI