Restriction map for
pF1KB6148
(Linear) (Six-base) MAPSORT of: KIBB6148.seq Check: 2147 from: 1 to: 1920
KIBB6148.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1544 1920
Size: 667 877 376
Fragments arranged by size:
877 667 376
AfeI AGC'GCT
Cuts at: 0 40 1920
Size: 40 1880
AflII C'TTAA_G
Cuts at: 0 1468 1920
Size: 1468 452
AhdI GACnn_n'nnGTC
Cuts at: 0 840 1920
Size: 840 1080
AlwNI CAG_nnn'CTG
Cuts at: 0 532 1920
Size: 532 1388
ApoI r'AATT_y
Cuts at: 0 147 203 494 753 1176 1512 1888 1920
Size: 147 56 291 259 423 336 376 32
Fragments arranged by size:
423 376 336 291 259 147 56 32
AvaI C'yCGr_G
Cuts at: 0 124 1920
Size: 124 1796
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1242 1275 1920
Size: 1242 33 645
Fragments arranged by size:
1242 645 33
BanI G'GyrC_C
Cuts at: 0 895 1920
Size: 895 1025
BciVI GTATCCnnnnn_n'
Cuts at: 0 814 1920
Size: 814 1106
BglI GCCn_nnn'nGGC
Cuts at: 0 649 1920
Size: 649 1271
BglII A'GATC_T
Cuts at: 0 1464 1920
Size: 1464 456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 341 667 1187 1488 1724 1920
Size: 297 44 326 520 301 236 196
Fragments arranged by size:
520 326 301 297 236 196 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 494 1205 1920
Size: 494 711 715
Fragments arranged by size:
715 711 494
BsaWI w'CCGG_w
Cuts at: 0 663 1920
Size: 663 1257
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 730 1174 1204 1920
Size: 700 30 444 30 716
Fragments arranged by size:
716 700 444 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 439 938 1154 1538 1920
Size: 46 393 499 216 384 382
Fragments arranged by size:
499 393 384 382 216 46
BseYI C'CCAG_C
Cuts at: 0 63 329 1920
Size: 63 266 1591
Fragments arranged by size:
1591 266 63
BsiHKAI G_wGCw'C
Cuts at: 0 860 1920
Size: 860 1060
BsmI GAATG_Cn'
Cuts at: 0 891 1920
Size: 891 1029
BsmBI CGTCTCn'nnnn_
Cuts at: 0 100 884 926 1611 1920
Size: 100 784 42 685 309
Fragments arranged by size:
784 685 309 100 42
Bsp1286I G_dGCh'C
Cuts at: 0 860 1920
Size: 860 1060
BspHI T'CATG_A
Cuts at: 0 1767 1920
Size: 1767 153
BssSI C'ACGA_G
Cuts at: 0 401 1920
Size: 401 1519
BstBI TT'CG_AA
Cuts at: 0 576 1920
Size: 576 1344
BstXI CCAn_nnnn'nTGG
Cuts at: 0 691 1920
Size: 691 1229
BstYI r'GATC_y
Cuts at: 0 1464 1920
Size: 1464 456
Bsu36I CC'TnA_GG
Cuts at: 0 278 1920
Size: 278 1642
BtgI C'CryG_G
Cuts at: 0 189 1550 1920
Size: 189 1361 370
Fragments arranged by size:
1361 370 189
BtsI GCAGTG_nn'
Cuts at: 0 1541 1920
Size: 1541 379
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 447 482 1920
Size: 447 35 1438
Fragments arranged by size:
1438 447 35
DraI TTT'AAA
Cuts at: 0 795 1920
Size: 795 1125
EarI CTCTTCn'nnn_
Cuts at: 0 1472 1920
Size: 1472 448
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 297 341 667 1187 1488 1544 1724 1920
Size: 297 44 326 520 301 56 180 196
Fragments arranged by size:
520 326 301 297 196 180 56 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 28 1920
Size: 28 1892
EcoO109I rG'GnC_Cy
Cuts at: 0 21 762 1920
Size: 21 741 1158
Fragments arranged by size:
1158 741 21
EcoRI G'AATT_C
Cuts at: 0 753 1920
Size: 753 1167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1656 1920
Size: 1656 264
HaeII r_GCGC'y
Cuts at: 0 42 124 1734 1920
Size: 42 82 1610 186
Fragments arranged by size:
1610 186 82 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 155 187 415 447 700 732 826 858
Size: 155 32 228 32 253 32 94 32
Cuts at: 858 925 957 1900 1920
Size: 67 32 943 20
Fragments arranged by size:
943 253 228 155 94 67 32 32
32 32 32 20
HincII GTy'rAC
Cuts at: 0 548 1236 1920
Size: 548 688 684
Fragments arranged by size:
688 684 548
MfeI C'AATT_G
Cuts at: 0 336 1565 1920
Size: 336 1229 355
Fragments arranged by size:
1229 355 336
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1211 1920
Size: 1211 709
MspA1I CmG'CkG
Cuts at: 0 1092 1131 1920
Size: 1092 39 789
Fragments arranged by size:
1092 789 39
NcoI C'CATG_G
Cuts at: 0 1550 1920
Size: 1550 370
NdeI CA'TA_TG
Cuts at: 0 474 1920
Size: 474 1446
NspI r_CATG'y
Cuts at: 0 94 1678 1920
Size: 94 1584 242
Fragments arranged by size:
1584 242 94
PasI CC'CwG_GG
Cuts at: 0 720 1920
Size: 720 1200
PpuMI rG'GwC_Cy
Cuts at: 0 762 1920
Size: 762 1158
PsiI TTA'TAA
Cuts at: 0 1631 1920
Size: 1631 289
PstI C_TGCA'G
Cuts at: 0 894 1083 1920
Size: 894 189 837
Fragments arranged by size:
894 837 189
SfcI C'TryA_G
Cuts at: 0 890 1079 1691 1920
Size: 890 189 612 229
Fragments arranged by size:
890 612 229 189
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 649 1920
Size: 649 1271
SmlI C'TyrA_G
Cuts at: 0 124 509 1220 1468 1920
Size: 124 385 711 248 452
Fragments arranged by size:
711 452 385 248 124
SphI G_CATG'C
Cuts at: 0 1678 1920
Size: 1678 242
StuI AGG'CCT
Cuts at: 0 652 1085 1920
Size: 652 433 835
Fragments arranged by size:
835 652 433
StyI C'CwwG_G
Cuts at: 0 180 1550 1920
Size: 180 1370 370
Fragments arranged by size:
1370 370 180
TatI w'GTAC_w
Cuts at: 0 1661 1920
Size: 1661 259
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1366 1398 1920
Size: 1366 32 522
Fragments arranged by size:
1366 522 32
XbaI T'CTAG_A
Cuts at: 0 862 1920
Size: 862 1058
XhoI C'TCGA_G
Cuts at: 0 124 1920
Size: 124 1796
XmnI GAAnn'nnTTC
Cuts at: 0 1847 1920
Size: 1847 73
Enzymes that do cut:
AcuI AfeI AflII AhdI AlwNI ApoI AvaI BaeI
BanI BciVI BglI BglII BpmI BpuEI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BssSI
BstBI BstXI BstYI Bsu36I BtgI BtsI CspCI DraI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I
HincII MfeI MmeI MspA1I NcoI NdeI NspI PasI
PpuMI PsiI PstI SfcI SfiI SmlI SphI StuI
StyI TatI TstI XbaI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflIII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstZ17I
BtgZI ClaI DraIII DrdI EaeI EagI EciI EcoICRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MluI MscI MslI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TaqII Tth111I XcmI XmaI ZraI