Restriction map for
pF1KB6143
(Linear) (Six-base) MAPSORT of: KIBB6143.seq Check: 306 from: 1 to: 1785
KIBB6143.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 976 1785
Size: 976 809
AfeI AGC'GCT
Cuts at: 0 1276 1785
Size: 1276 509
AflIII A'CryG_T
Cuts at: 0 1637 1785
Size: 1637 148
AhdI GACnn_n'nnGTC
Cuts at: 0 321 1785
Size: 321 1464
AlwNI CAG_nnn'CTG
Cuts at: 0 223 475 1785
Size: 223 252 1310
Fragments arranged by size:
1310 252 223
ApaI G_GGCC'C
Cuts at: 0 381 515 1288 1785
Size: 381 134 773 497
Fragments arranged by size:
773 497 381 134
ApoI r'AATT_y
Cuts at: 0 173 213 605 619 692 964 1062 1785
Size: 173 40 392 14 73 272 98 723
Fragments arranged by size:
723 392 272 173 98 73 40 14
AvaI C'yCGr_G
Cuts at: 0 14 1785
Size: 14 1771
BanI G'GyrC_C
Cuts at: 0 1158 1785
Size: 1158 627
BanII G_rGCy'C
Cuts at: 0 381 402 515 1288 1785
Size: 381 21 113 773 497
Fragments arranged by size:
773 497 381 113 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 506 1785
Size: 506 1279
BbvCI CC'TCA_GC
Cuts at: 0 1753 1765 1785
Size: 1753 12 20
Fragments arranged by size:
1753 20 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1723 1757 1785
Size: 1723 34 28
Fragments arranged by size:
1723 34 28
BglI GCCn_nnn'nGGC
Cuts at: 0 10 1785
Size: 10 1775
BglII A'GATC_T
Cuts at: 0 1374 1452 1785
Size: 1374 78 333
Fragments arranged by size:
1374 333 78
BlpI GC'TnA_GC
Cuts at: 0 566 1785
Size: 566 1219
Bme1580I G_kGCm'C
Cuts at: 0 381 515 1288 1785
Size: 381 134 773 497
Fragments arranged by size:
773 497 381 134
BmrI ACTGGGnnnn_n'
Cuts at: 0 176 363 438 501 513 1785
Size: 176 187 75 63 12 1272
Fragments arranged by size:
1272 187 176 75 63 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 416 1732 1785
Size: 351 65 1316 53
Fragments arranged by size:
1316 351 65 53
Bpu10I CC'TnA_GC
Cuts at: 0 263 1753 1765 1785
Size: 263 1490 12 20
Fragments arranged by size:
1490 263 20 12
BsaWI w'CCGG_w
Cuts at: 0 96 1673 1785
Size: 96 1577 112
Fragments arranged by size:
1577 112 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 402 432 1785
Size: 402 30 1353
Fragments arranged by size:
1353 402 30
BseYI C'CCAG_C
Cuts at: 0 371 380 1785
Size: 371 9 1405
Fragments arranged by size:
1405 371 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1263 1785
Size: 1263 522
BsiHKAI G_wGCw'C
Cuts at: 0 1268 1785
Size: 1268 517
BsmBI CGTCTCn'nnnn_
Cuts at: 0 892 1785
Size: 892 893
Bsp1286I G_dGCh'C
Cuts at: 0 381 402 515 1268 1288 1785
Size: 381 21 113 753 20 497
Fragments arranged by size:
753 497 381 113 21 20
BspEI T'CCGG_A
Cuts at: 0 96 1785
Size: 96 1689
BspHI T'CATG_A
Cuts at: 0 1130 1785
Size: 1130 655
BsrDI GCAATG_nn'
Cuts at: 0 318 1785
Size: 318 1467
BssSI C'ACGA_G
Cuts at: 0 1429 1480 1785
Size: 1429 51 305
Fragments arranged by size:
1429 305 51
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1722 1785
Size: 1722 63
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1224 1431 1785
Size: 1224 207 354
Fragments arranged by size:
1224 354 207
BstYI r'GATC_y
Cuts at: 0 765 1374 1452 1676 1785
Size: 765 609 78 224 109
Fragments arranged by size:
765 609 224 109 78
BtgI C'CryG_G
Cuts at: 0 1145 1223 1785
Size: 1145 78 562
Fragments arranged by size:
1145 562 78
BtsI GCAGTG_nn'
Cuts at: 0 312 434 1785
Size: 312 122 1351
Fragments arranged by size:
1351 312 122
ClaI AT'CG_AT
Cuts at: 0 840 1785
Size: 840 945
DrdI GACnn_nn'nnGTC
Cuts at: 0 940 1785
Size: 940 845
EaeI y'GGCC_r
Cuts at: 0 2 444 662 1334 1785
Size: 2 442 218 672 451
Fragments arranged by size:
672 451 442 218 2
EarI CTCTTCn'nnn_
Cuts at: 0 528 932 953 1609 1785
Size: 528 404 21 656 176
Fragments arranged by size:
656 528 404 176 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1690 1785
Size: 1690 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 351 416 976 1732 1785
Size: 351 65 560 756 53
Fragments arranged by size:
756 560 351 65 53
EcoO109I rG'GnC_Cy
Cuts at: 0 377 1761 1785
Size: 377 1384 24
Fragments arranged by size:
1384 377 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 253 1513 1785
Size: 253 1260 272
Fragments arranged by size:
1260 272 253
FspI TGC'GCA
Cuts at: 0 979 1785
Size: 979 806
FspAI rTGC'GCAy
Cuts at: 0 979 1785
Size: 979 806
HaeII r_GCGC'y
Cuts at: 0 566 1278 1785
Size: 566 712 507
Fragments arranged by size:
712 566 507
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 232 264 307 339 529 561
Size: 65 32 135 32 43 32 190 32
Cuts at: 561 1610 1642 1785
Size: 1049 32 143
Fragments arranged by size:
1049 190 143 135 65 43 32 32
32 32 32
MluI A'CGCG_T
Cuts at: 0 1637 1785
Size: 1637 148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1785
Size: 687 1098
MscI TGG'CCA
Cuts at: 0 4 664 1336 1785
Size: 4 660 672 449
Fragments arranged by size:
672 660 449 4
MspA1I CmG'CkG
Cuts at: 0 550 1785
Size: 550 1235
NcoI C'CATG_G
Cuts at: 0 1145 1223 1785
Size: 1145 78 562
Fragments arranged by size:
1145 562 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 428 475 1785
Size: 428 47 1310
Fragments arranged by size:
1310 428 47
PfoI T'CCnGG_A
Cuts at: 0 624 1785
Size: 624 1161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 137 169 1785
Size: 137 32 1616
Fragments arranged by size:
1616 137 32
PspOMI G'GGCC_C
Cuts at: 0 377 511 1284 1785
Size: 377 134 773 501
Fragments arranged by size:
773 501 377 134
PvuII CAG'CTG
Cuts at: 0 550 1785
Size: 550 1235
SexAI A'CCwGG_T
Cuts at: 0 296 1785
Size: 296 1489
SpeI A'CTAG_T
Cuts at: 0 1566 1785
Size: 1566 219
StuI AGG'CCT
Cuts at: 0 457 997 1321 1785
Size: 457 540 324 464
Fragments arranged by size:
540 464 457 324
StyI C'CwwG_G
Cuts at: 0 8 462 1145 1217 1223 1295 1785
Size: 8 454 683 72 6 72 490
Fragments arranged by size:
683 490 454 72 72 8 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 203 235 402 434 1785
Size: 203 32 167 32 1351
Fragments arranged by size:
1351 203 167 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 304 1785
Size: 304 1481
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BsaWI BsaXI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BsrDI BssSI BstAPI
BstXI BstYI BtgI BtsI ClaI DrdI EaeI EarI
EciI Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I
MluI MmeI MscI MspA1I NcoI PflMI PfoI PpiI
PspOMI PvuII SexAI SpeI StuI StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsiEI BsiWI
BsmI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI CspCI DraI DraIII EagI EcoICRI
EcoNI EcoRI EcoRV FseI HincII HindIII HpaI KasI
KpnI MfeI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PmeI
PmlI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SphI SrfI SspI SwaI TaqII TatI Tth111I XbaI
XhoI XmaI XmnI ZraI