Restriction map for
pF1KB6134
(Linear) (Six-base) MAPSORT of: KIBB6134.seq Check: 6562 from: 1 to: 1893
KIBB6134.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 0 958 1893
Size: 958 935
AccI GT'mk_AC
Cuts at: 0 626 1893
Size: 626 1267
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 994 1267 1429 1447 1893
Size: 673 321 273 162 18 446
Fragments arranged by size:
673 446 321 273 162 18
AfeI AGC'GCT
Cuts at: 0 832 1444 1893
Size: 832 612 449
Fragments arranged by size:
832 612 449
AleI CACnn'nnGTG
Cuts at: 0 1615 1893
Size: 1615 278
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 813 1893
Size: 34 779 1080
Fragments arranged by size:
1080 779 34
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 695 727 1893
Size: 695 32 1166
Fragments arranged by size:
1166 695 32
AlwNI CAG_nnn'CTG
Cuts at: 0 542 552 1056 1089 1533 1893
Size: 542 10 504 33 444 360
Fragments arranged by size:
542 504 444 360 33 10
AvaI C'yCGr_G
Cuts at: 0 1186 1217 1893
Size: 1186 31 676
Fragments arranged by size:
1186 676 31
BamHI G'GATC_C
Cuts at: 0 1765 1875 1893
Size: 1765 110 18
Fragments arranged by size:
1765 110 18
BanI G'GyrC_C
Cuts at: 0 365 680 1013 1151 1893
Size: 365 315 333 138 742
Fragments arranged by size:
742 365 333 315 138
BanII G_rGCy'C
Cuts at: 0 536 560 638 1893
Size: 536 24 78 1255
Fragments arranged by size:
1255 536 78 24
BbeI G_GCGC'C
Cuts at: 0 1017 1155 1893
Size: 1017 138 738
Fragments arranged by size:
1017 738 138
BbsI GAAGACnn'nnnn_
Cuts at: 0 856 958 1893
Size: 856 102 935
Fragments arranged by size:
935 856 102
BbvCI CC'TCA_GC
Cuts at: 0 604 1893
Size: 604 1289
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 918 952 1893
Size: 918 34 941
Fragments arranged by size:
941 918 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 193 1893
Size: 193 1700
BclI T'GATC_A
Cuts at: 0 404 1893
Size: 404 1489
BfrBI ATG'CAT
Cuts at: 0 1653 1893
Size: 1653 240
BglI GCCn_nnn'nGGC
Cuts at: 0 505 514 584 1893
Size: 505 9 70 1309
Fragments arranged by size:
1309 505 70 9
BlpI GC'TnA_GC
Cuts at: 0 535 1893
Size: 535 1358
Bme1580I G_kGCm'C
Cuts at: 0 683 1893
Size: 683 1210
BmgBI CAC'GTC
Cuts at: 0 57 1893
Size: 57 1836
BmrI ACTGGGnnnn_n'
Cuts at: 0 705 1893
Size: 705 1188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 505 574 1111 1312 1555 1688 1893
Size: 172 333 69 537 201 243 133 205
Fragments arranged by size:
537 333 243 205 201 172 133 69
Bpu10I CC'TnA_GC
Cuts at: 0 604 1893
Size: 604 1289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 7 76 1893
Size: 7 69 1817
Fragments arranged by size:
1817 69 7
BsaAI yAC'GTr
Cuts at: 0 866 1893
Size: 866 1027
BsaBI GATnn'nnATC
Cuts at: 0 928 1893
Size: 928 965
BsaHI Gr'CG_yC
Cuts at: 0 955 1014 1152 1893
Size: 955 59 138 741
Fragments arranged by size:
955 741 138 59
BsaWI w'CCGG_w
Cuts at: 0 1680 1893
Size: 1680 213
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1720 1750 1893
Size: 1720 30 143
Fragments arranged by size:
1720 143 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 256 502 1048 1078 1153 1156 1267 1270
Size: 256 246 546 30 75 3 111 3
Cuts at: 1270 1309 1408 1447 1552 1728 1893
Size: 39 99 39 105 176 165
Fragments arranged by size:
546 256 246 176 165 111 105 99
75 39 39 30 3 3
BseYI C'CCAG_C
Cuts at: 0 86 724 1523 1760 1893
Size: 86 638 799 237 133
Fragments arranged by size:
799 638 237 133 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1893
Size: 109 1784
BsiHKAI G_wGCw'C
Cuts at: 0 266 560 638 1893
Size: 266 294 78 1255
Fragments arranged by size:
1255 294 266 78
Bsp1286I G_dGCh'C
Cuts at: 0 266 536 560 638 683 1893
Size: 266 270 24 78 45 1210
Fragments arranged by size:
1210 270 266 78 45 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 440 1893
Size: 440 1453
BsrBI CCG'CTC
Cuts at: 0 1011 1074 1125 1508 1893
Size: 1011 63 51 383 385
Fragments arranged by size:
1011 385 383 63 51
BsrDI GCAATG_nn'
Cuts at: 0 1806 1893
Size: 1806 87
BsrFI r'CCGG_y
Cuts at: 0 1553 1643 1893
Size: 1553 90 250
Fragments arranged by size:
1553 250 90
BsrGI T'GTAC_A
Cuts at: 0 113 1893
Size: 113 1780
BssSI C'ACGA_G
Cuts at: 0 619 1893
Size: 619 1274
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1056 1311 1419 1893
Size: 1056 255 108 474
Fragments arranged by size:
1056 474 255 108
BstEII G'GTnAC_C
Cuts at: 0 1683 1893
Size: 1683 210
BstXI CCAn_nnnn'nTGG
Cuts at: 0 924 1386 1893
Size: 924 462 507
Fragments arranged by size:
924 507 462
BstYI r'GATC_y
Cuts at: 0 1765 1875 1893
Size: 1765 110 18
Fragments arranged by size:
1765 110 18
Bsu36I CC'TnA_GG
Cuts at: 0 1859 1893
Size: 1859 34
BtgI C'CryG_G
Cuts at: 0 143 1893
Size: 143 1750
BtsI GCAGTG_nn'
Cuts at: 0 792 1893
Size: 792 1101
EaeI y'GGCC_r
Cuts at: 0 293 611 1022 1893
Size: 293 318 411 871
Fragments arranged by size:
871 411 318 293
EarI CTCTTCn'nnn_
Cuts at: 0 58 376 565 746 1025 1385 1394 1469
Size: 58 318 189 181 279 360 9 75
Cuts at: 1469 1893
Size: 424
Fragments arranged by size:
424 360 318 279 189 181 75 58
9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 346 1222 1893
Size: 346 876 671
Fragments arranged by size:
876 671 346
EcoICRI GAG'CTC
Cuts at: 0 558 636 1893
Size: 558 78 1257
Fragments arranged by size:
1257 558 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 505 574 673 994 1111 1267 1312
Size: 172 333 69 99 321 117 156 45
Cuts at: 1312 1429 1447 1555 1688 1893
Size: 117 18 108 133 205
Fragments arranged by size:
333 321 205 172 156 133 117 117
108 99 69 45 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 10 232 385 655 964 1516 1893
Size: 10 222 153 270 309 552 377
Fragments arranged by size:
552 377 309 270 222 153 10
EcoO109I rG'GnC_Cy
Cuts at: 0 969 1320 1781 1893
Size: 969 351 461 112
Fragments arranged by size:
969 461 351 112
FspI TGC'GCA
Cuts at: 0 10 1893
Size: 10 1883
HaeII r_GCGC'y
Cuts at: 0 834 881 900 1017 1155 1186 1446 1752
Size: 834 47 19 117 138 31 260 306
Cuts at: 1752 1893
Size: 141
Fragments arranged by size:
834 306 260 141 138 117 47 31
19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1342 1374 1893
Size: 1342 32 519
Fragments arranged by size:
1342 519 32
KasI G'GCGC_C
Cuts at: 0 1013 1151 1893
Size: 1013 138 742
Fragments arranged by size:
1013 742 138
MfeI C'AATT_G
Cuts at: 0 1658 1893
Size: 1658 235
MscI TGG'CCA
Cuts at: 0 613 1893
Size: 613 1280
MslI CAynn'nnrTG
Cuts at: 0 269 922 1615 1628 1893
Size: 269 653 693 13 265
Fragments arranged by size:
693 653 269 265 13
MspA1I CmG'CkG
Cuts at: 0 575 717 728 783 913 996 1005 1056
Size: 575 142 11 55 130 83 9 51
Cuts at: 1056 1113 1272 1308 1425 1533 1760 1893
Size: 57 159 36 117 108 227 133
Fragments arranged by size:
575 227 159 142 133 130 117 108
83 57 55 51 36 11 9
NarI GG'CG_CC
Cuts at: 0 1014 1152 1893
Size: 1014 138 741
Fragments arranged by size:
1014 741 138
NsiI A_TGCA'T
Cuts at: 0 1655 1893
Size: 1655 238
NspI r_CATG'y
Cuts at: 0 207 840 1893
Size: 207 633 1053
Fragments arranged by size:
1053 633 207
PasI CC'CwG_GG
Cuts at: 0 1770 1893
Size: 1770 123
PfoI T'CCnGG_A
Cuts at: 0 227 551 1893
Size: 227 324 1342
Fragments arranged by size:
1342 324 227
PpuMI rG'GwC_Cy
Cuts at: 0 969 1781 1893
Size: 969 812 112
Fragments arranged by size:
969 812 112
PspXI vC'TCGA_Gb
Cuts at: 0 1186 1893
Size: 1186 707
PstI C_TGCA'G
Cuts at: 0 722 941 1052 1061 1085 1103 1136 1178
Size: 722 219 111 9 24 18 33 42
Cuts at: 1178 1484 1517 1893
Size: 306 33 376
Fragments arranged by size:
722 376 306 219 111 42 33 33
24 18 9
PvuII CAG'CTG
Cuts at: 0 717 728 913 1056 1425 1533 1893
Size: 717 11 185 143 369 108 360
Fragments arranged by size:
717 369 360 185 143 108 11
SacI G_AGCT'C
Cuts at: 0 560 638 1893
Size: 560 78 1255
Fragments arranged by size:
1255 560 78
SapI GCTCTTCn'nnn_
Cuts at: 0 565 1469 1893
Size: 565 904 424
Fragments arranged by size:
904 565 424
SfcI C'TryA_G
Cuts at: 0 718 937 1048 1057 1081 1099 1132 1174
Size: 718 219 111 9 24 18 33 42
Cuts at: 1174 1480 1513 1893
Size: 306 33 380
Fragments arranged by size:
718 380 306 219 111 42 33 33
24 18 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 505 514 1893
Size: 505 9 1379
Fragments arranged by size:
1379 505 9
SfoI GGC'GCC
Cuts at: 0 1015 1153 1893
Size: 1015 138 740
Fragments arranged by size:
1015 740 138
SmaI CCC'GGG
Cuts at: 0 1219 1893
Size: 1219 674
SmlI C'TyrA_G
Cuts at: 0 22 91 1186 1893
Size: 22 69 1095 707
Fragments arranged by size:
1095 707 69 22
SrfI GCCC'GGGC
Cuts at: 0 1219 1893
Size: 1219 674
StyI C'CwwG_G
Cuts at: 0 1025 1893
Size: 1025 868
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 466 1893
Size: 466 1427
TatI w'GTAC_w
Cuts at: 0 113 221 1893
Size: 113 108 1672
Fragments arranged by size:
1672 113 108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1720 1752 1893
Size: 1720 32 141
Fragments arranged by size:
1720 141 32
XhoI C'TCGA_G
Cuts at: 0 1186 1893
Size: 1186 707
XmaI C'CCGG_G
Cuts at: 0 1217 1893
Size: 1217 676
ZraI GAC'GTC
Cuts at: 0 956 1893
Size: 956 937
Enzymes that do cut:
AatII AccI AcuI AfeI AleI AlfI AloI AlwNI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI MfeI MscI MslI
MspA1I NarI NsiI NspI PasI PfoI PpuMI PspXI
PstI PvuII SacI SapI SfcI SfiI SfoI SmaI
SmlI SrfI StyI TaqII TatI TstI XhoI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI ApaI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BglII
BmtI BplI BsaI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI Tth111I
XbaI XcmI XmnI