Restriction map for
pF1KB6128
(Linear) (Six-base) MAPSORT of: KIBB6128.seq Check: 1752 from: 1 to: 1881
KIBB6128.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 349 1881
Size: 349 1532
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1174 1412 1546 1881
Size: 1174 238 134 335
Fragments arranged by size:
1174 335 238 134
AleI CACnn'nnGTG
Cuts at: 0 931 1373 1881
Size: 931 442 508
Fragments arranged by size:
931 508 442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1756 1881
Size: 1756 125
AlwNI CAG_nnn'CTG
Cuts at: 0 741 1099 1146 1881
Size: 741 358 47 735
Fragments arranged by size:
741 735 358 47
ApaI G_GGCC'C
Cuts at: 0 795 1881
Size: 795 1086
ApoI r'AATT_y
Cuts at: 0 398 1881
Size: 398 1483
AvaI C'yCGr_G
Cuts at: 0 806 1000 1759 1881
Size: 806 194 759 122
Fragments arranged by size:
806 759 194 122
AvrII C'CTAG_G
Cuts at: 0 1860 1881
Size: 1860 21
BanI G'GyrC_C
Cuts at: 0 585 1732 1881
Size: 585 1147 149
Fragments arranged by size:
1147 585 149
BanII G_rGCy'C
Cuts at: 0 151 472 729 795 871 914 1001 1836
Size: 151 321 257 66 76 43 87 835
Cuts at: 1836 1881
Size: 45
Fragments arranged by size:
835 321 257 151 87 76 66 45
43
BbsI GAAGACnn'nnnn_
Cuts at: 0 1072 1798 1881
Size: 1072 726 83
Fragments arranged by size:
1072 726 83
BbvCI CC'TCA_GC
Cuts at: 0 157 590 1798 1881
Size: 157 433 1208 83
Fragments arranged by size:
1208 433 157 83
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 489 523 543 577 610 644 1171 1205
Size: 489 34 20 34 33 34 527 34
Cuts at: 1205 1881
Size: 676
Fragments arranged by size:
676 527 489 34 34 34 34 33
20
BclI T'GATC_A
Cuts at: 0 131 1881
Size: 131 1750
BglI GCCn_nnn'nGGC
Cuts at: 0 44 49 1862 1881
Size: 44 5 1813 19
Fragments arranged by size:
1813 44 19 5
BglII A'GATC_T
Cuts at: 0 1538 1881
Size: 1538 343
BlpI GC'TnA_GC
Cuts at: 0 314 728 1881
Size: 314 414 1153
Fragments arranged by size:
1153 414 314
Bme1580I G_kGCm'C
Cuts at: 0 795 1233 1881
Size: 795 438 648
Fragments arranged by size:
795 648 438
BmrI ACTGGGnnnn_n'
Cuts at: 0 269 690 1881
Size: 269 421 1191
Fragments arranged by size:
1191 421 269
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 230 1881
Size: 230 1651
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 448 463 928 1462 1513 1828 1881
Size: 44 404 15 465 534 51 315 53
Fragments arranged by size:
534 465 404 315 53 51 44 15
Bpu10I CC'TnA_GC
Cuts at: 0 157 215 590 986 1798 1881
Size: 157 58 375 396 812 83
Fragments arranged by size:
812 396 375 157 83 58
BsaI GGTCTCn'nnnn_
Cuts at: 0 287 1748 1881
Size: 287 1461 133
Fragments arranged by size:
1461 287 133
BsaAI yAC'GTr
Cuts at: 0 977 1881
Size: 977 904
BsaHI Gr'CG_yC
Cuts at: 0 1076 1881
Size: 1076 805
BsaWI w'CCGG_w
Cuts at: 0 689 1511 1598 1881
Size: 689 822 87 283
Fragments arranged by size:
822 689 283 87
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 843 873 988 1018 1881
Size: 843 30 115 30 863
Fragments arranged by size:
863 843 115 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 367 607 1060 1273 1426 1777 1881
Size: 19 348 240 453 213 153 351 104
Fragments arranged by size:
453 351 348 240 213 153 104 19
BseYI C'CCAG_C
Cuts at: 0 776 841 1232 1481 1782 1881
Size: 776 65 391 249 301 99
Fragments arranged by size:
776 391 301 249 99 65
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 88 1108 1351 1672 1881
Size: 88 1020 243 321 209
Fragments arranged by size:
1020 321 243 209 88
BsiHKAI G_wGCw'C
Cuts at: 0 861 1001 1881
Size: 861 140 880
Fragments arranged by size:
880 861 140
Bsp1286I G_dGCh'C
Cuts at: 0 151 472 729 795 861 871 914 1001
Size: 151 321 257 66 66 10 43 87
Cuts at: 1001 1233 1836 1881
Size: 232 603 45
Fragments arranged by size:
603 321 257 232 151 87 66 66
45 43 10
BspEI T'CCGG_A
Cuts at: 0 1511 1881
Size: 1511 370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 349 1881
Size: 305 44 1532
Fragments arranged by size:
1532 305 44
BsrFI r'CCGG_y
Cuts at: 0 43 662 1106 1881
Size: 43 619 444 775
Fragments arranged by size:
775 619 444 43
BssHII G'CGCG_C
Cuts at: 0 78 1881
Size: 78 1803
BssSI C'ACGA_G
Cuts at: 0 1690 1881
Size: 1690 191
BstAPI GCAn_nnn'nTGC
Cuts at: 0 75 741 1146 1881
Size: 75 666 405 735
Fragments arranged by size:
735 666 405 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 210 504 1881
Size: 96 114 294 1377
Fragments arranged by size:
1377 294 114 96
BstYI r'GATC_y
Cuts at: 0 1355 1538 1814 1881
Size: 1355 183 276 67
Fragments arranged by size:
1355 276 183 67
Bsu36I CC'TnA_GG
Cuts at: 0 863 1881
Size: 863 1018
BtgI C'CryG_G
Cuts at: 0 538 1881
Size: 538 1343
BtsI GCAGTG_nn'
Cuts at: 0 415 1881
Size: 415 1466
EaeI y'GGCC_r
Cuts at: 0 200 664 1881
Size: 200 464 1217
Fragments arranged by size:
1217 464 200
EarI CTCTTCn'nnn_
Cuts at: 0 310 1150 1388 1520 1881
Size: 310 840 238 132 361
Fragments arranged by size:
840 361 310 238 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 184 1828 1881
Size: 25 159 1644 53
Fragments arranged by size:
1644 159 53 25
EcoICRI GAG'CTC
Cuts at: 0 999 1881
Size: 999 882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 44 448 463 928 1174 1412 1462 1513
Size: 44 404 15 465 246 238 50 51
Cuts at: 1513 1546 1828 1881
Size: 33 282 53
Fragments arranged by size:
465 404 282 246 238 53 51 50
44 33 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 214 1840 1881
Size: 214 1626 41
Fragments arranged by size:
1626 214 41
EcoO109I rG'GnC_Cy
Cuts at: 0 426 780 791 792 810 1143 1275 1881
Size: 426 354 11 1 18 333 132 606
Fragments arranged by size:
606 426 354 333 132 18 11 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 915 1881
Size: 915 966
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 747 779 843 875 988 1020 1318 1350
Size: 747 32 64 32 113 32 298 32
Cuts at: 1350 1881
Size: 531
Fragments arranged by size:
747 531 298 113 64 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 1881
Size: 579 1302
MscI TGG'CCA
Cuts at: 0 202 1881
Size: 202 1679
MslI CAynn'nnrTG
Cuts at: 0 931 1373 1738 1881
Size: 931 442 365 143
Fragments arranged by size:
931 442 365 143
MspA1I CmG'CkG
Cuts at: 0 119 411 732 738 1020 1236 1428 1881
Size: 119 292 321 6 282 216 192 453
Fragments arranged by size:
453 321 292 282 216 192 119 6
NaeI GCC'GGC
Cuts at: 0 45 1108 1881
Size: 45 1063 773
Fragments arranged by size:
1063 773 45
NgoMIV G'CCGG_C
Cuts at: 0 43 1106 1881
Size: 43 1063 775
Fragments arranged by size:
1063 775 43
PasI CC'CwG_GG
Cuts at: 0 1071 1881
Size: 1071 810
PfoI T'CCnGG_A
Cuts at: 0 1490 1817 1871 1881
Size: 1490 327 54 10
Fragments arranged by size:
1490 327 54 10
PmlI CAC'GTG
Cuts at: 0 977 1881
Size: 977 904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 983 1015 1612 1644 1881
Size: 983 32 597 32 237
Fragments arranged by size:
983 597 237 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 426 810 1275 1881
Size: 426 384 465 606
Fragments arranged by size:
606 465 426 384
PspOMI G'GGCC_C
Cuts at: 0 791 1881
Size: 791 1090
PspXI vC'TCGA_Gb
Cuts at: 0 1000 1759 1881
Size: 1000 759 122
Fragments arranged by size:
1000 759 122
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 253 285 1881
Size: 253 32 1596
Fragments arranged by size:
1596 253 32
PstI C_TGCA'G
Cuts at: 0 360 458 638 746 830 1211 1316 1702
Size: 360 98 180 108 84 381 105 386
Cuts at: 1702 1881
Size: 179
Fragments arranged by size:
386 381 360 180 179 108 105 98
84
PvuII CAG'CTG
Cuts at: 0 411 1020 1428 1881
Size: 411 609 408 453
Fragments arranged by size:
609 453 411 408
RsrII CG'GwC_CG
Cuts at: 0 12 1881
Size: 12 1869
SacI G_AGCT'C
Cuts at: 0 1001 1881
Size: 1001 880
SanDI GG'GwC_CC
Cuts at: 0 426 1275 1881
Size: 426 849 606
Fragments arranged by size:
849 606 426
SapI GCTCTTCn'nnn_
Cuts at: 0 1150 1881
Size: 1150 731
SbfI CC_TGCA'GG
Cuts at: 0 360 1881
Size: 360 1521
SfcI C'TryA_G
Cuts at: 0 356 454 634 742 826 1207 1312 1698
Size: 356 98 180 108 84 381 105 386
Cuts at: 1698 1881
Size: 183
Fragments arranged by size:
386 381 356 183 180 108 105 98
84
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1862 1881
Size: 1862 19
SmaI CCC'GGG
Cuts at: 0 808 1881
Size: 808 1073
SmlI C'TyrA_G
Cuts at: 0 1000 1759 1881
Size: 1000 759 122
Fragments arranged by size:
1000 759 122
StuI AGG'CCT
Cuts at: 0 220 832 1666 1881
Size: 220 612 834 215
Fragments arranged by size:
834 612 220 215
StyI C'CwwG_G
Cuts at: 0 242 421 1860 1881
Size: 242 179 1439 21
Fragments arranged by size:
1439 242 179 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 29 1495 1881
Size: 29 1466 386
Fragments arranged by size:
1466 386 29
TatI w'GTAC_w
Cuts at: 0 1123 1881
Size: 1123 758
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 39 71 530 562 679 711 1881
Size: 39 32 459 32 117 32 1170
Fragments arranged by size:
1170 459 117 39 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 682 1881
Size: 682 1199
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1849 1881
Size: 1849 32
XhoI C'TCGA_G
Cuts at: 0 1000 1759 1881
Size: 1000 759 122
Fragments arranged by size:
1000 759 122
XmaI C'CCGG_G
Cuts at: 0 806 1881
Size: 806 1075
XmnI GAAnn'nnTTC
Cuts at: 0 487 1881
Size: 487 1394
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI ApoI AvaI
AvrII BanI BanII BbsI BbvCI BcgI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrFI BssHII BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI Hin4I MmeI MscI MslI
MspA1I NaeI NgoMIV PasI PfoI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuII RsrII SacI SanDI
SapI SbfI SfcI SfiI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaLI AscI AseI AsiSI BaeI BamHI
BbeI BciVI BfrBI BmgBI BmtI BpuEI BsaBI BsiEI
BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrGI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PshAI PsiI PvuI SacII SalI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI ZraI