Restriction map for
pF1KB6127
(Linear) (Six-base) MAPSORT of: KIBB6127.seq Check: 1022 from: 1 to: 1761
KIBB6127.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 0 1178 1761
Size: 1178 583
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1594 1761
Size: 1594 167
AflIII A'CryG_T
Cuts at: 0 767 1124 1761
Size: 767 357 637
Fragments arranged by size:
767 637 357
AhdI GACnn_n'nnGTC
Cuts at: 0 768 1761
Size: 768 993
AlwNI CAG_nnn'CTG
Cuts at: 0 624 921 1233 1761
Size: 624 297 312 528
Fragments arranged by size:
624 528 312 297
ApaI G_GGCC'C
Cuts at: 0 99 1520 1761
Size: 99 1421 241
Fragments arranged by size:
1421 241 99
AvaI C'yCGr_G
Cuts at: 0 92 467 1057 1761
Size: 92 375 590 704
Fragments arranged by size:
704 590 375 92
BanI G'GyrC_C
Cuts at: 0 165 835 1053 1156 1761
Size: 165 670 218 103 605
Fragments arranged by size:
670 605 218 165 103
BanII G_rGCy'C
Cuts at: 0 99 257 1367 1460 1520 1761
Size: 99 158 1110 93 60 241
Fragments arranged by size:
1110 241 158 99 93 60
BbeI G_GCGC'C
Cuts at: 0 169 1057 1160 1761
Size: 169 888 103 601
Fragments arranged by size:
888 601 169 103
BbsI GAAGACnn'nnnn_
Cuts at: 0 226 1085 1761
Size: 226 859 676
Fragments arranged by size:
859 676 226
BclI T'GATC_A
Cuts at: 0 227 1761
Size: 227 1534
BglI GCCn_nnn'nGGC
Cuts at: 0 308 959 1385 1761
Size: 308 651 426 376
Fragments arranged by size:
651 426 376 308
BlpI GC'TnA_GC
Cuts at: 0 1021 1761
Size: 1021 740
Bme1580I G_kGCm'C
Cuts at: 0 99 1520 1761
Size: 99 1421 241
Fragments arranged by size:
1421 241 99
BmgBI CAC'GTC
Cuts at: 0 1038 1761
Size: 1038 723
BmrI ACTGGGnnnn_n'
Cuts at: 0 1611 1761
Size: 1611 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 628 1030 1404 1761
Size: 42 586 402 374 357
Fragments arranged by size:
586 402 374 357 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 1315 1761
Size: 735 580 446
Fragments arranged by size:
735 580 446
BsaAI yAC'GTr
Cuts at: 0 1545 1761
Size: 1545 216
BsaHI Gr'CG_yC
Cuts at: 0 166 842 962 1054 1157 1175 1259 1761
Size: 166 676 120 92 103 18 84 502
Fragments arranged by size:
676 502 166 120 103 92 84 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 57 87 629 659 871 901 1761
Size: 57 30 542 30 212 30 860
Fragments arranged by size:
860 542 212 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 650 820 1761
Size: 650 170 941
Fragments arranged by size:
941 650 170
BseYI C'CCAG_C
Cuts at: 0 158 1459 1508 1761
Size: 158 1301 49 253
Fragments arranged by size:
1301 253 158 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 53 265 417 550 565 862 934
Size: 35 18 212 152 133 15 297 72
Cuts at: 934 958 1489 1576 1761
Size: 24 531 87 185
Fragments arranged by size:
531 297 212 185 152 133 87 72
35 24 18 15
BsiEI CG_ry'CG
Cuts at: 0 986 1448 1761
Size: 986 462 313
Fragments arranged by size:
986 462 313
BsmI GAATG_Cn'
Cuts at: 0 663 1761
Size: 663 1098
Bsp1286I G_dGCh'C
Cuts at: 0 99 257 1367 1460 1520 1761
Size: 99 158 1110 93 60 241
Fragments arranged by size:
1110 241 158 99 93 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1415 1761
Size: 1415 346
BsrBI CCG'CTC
Cuts at: 0 480 577 632 1716 1761
Size: 480 97 55 1084 45
Fragments arranged by size:
1084 480 97 55 45
BsrDI GCAATG_nn'
Cuts at: 0 606 1761
Size: 606 1155
BsrFI r'CCGG_y
Cuts at: 0 87 927 1082 1404 1761
Size: 87 840 155 322 357
Fragments arranged by size:
840 357 322 155 87
BssHII G'CGCG_C
Cuts at: 0 1025 1027 1062 1064 1066 1761
Size: 1025 2 35 2 2 695
Fragments arranged by size:
1025 695 35 2 2 2
BssSI C'ACGA_G
Cuts at: 0 283 583 1192 1761
Size: 283 300 609 569
Fragments arranged by size:
609 569 300 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 921 1233 1761
Size: 921 312 528
Fragments arranged by size:
921 528 312
BstBI TT'CG_AA
Cuts at: 0 1668 1761
Size: 1668 93
BstEII G'GTnAC_C
Cuts at: 0 930 1761
Size: 930 831
BstYI r'GATC_y
Cuts at: 0 620 1761
Size: 620 1141
BtgI C'CryG_G
Cuts at: 0 564 1206 1242 1442 1761
Size: 564 642 36 200 319
Fragments arranged by size:
642 564 319 200 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 218 867 1761
Size: 218 649 894
Fragments arranged by size:
894 649 218
BtsI GCAGTG_nn'
Cuts at: 0 65 424 1761
Size: 65 359 1337
Fragments arranged by size:
1337 359 65
ClaI AT'CG_AT
Cuts at: 0 1526 1761
Size: 1526 235
DraIII CAC_nnn'GTG
Cuts at: 0 835 1761
Size: 835 926
DrdI GACnn_nn'nnGTC
Cuts at: 0 610 1606 1761
Size: 610 996 155
Fragments arranged by size:
996 610 155
EaeI y'GGCC_r
Cuts at: 0 300 686 983 1196 1445 1761
Size: 300 386 297 213 249 316
Fragments arranged by size:
386 316 300 297 249 213
EagI C'GGCC_G
Cuts at: 0 983 1445 1761
Size: 983 462 316
Fragments arranged by size:
983 462 316
EarI CTCTTCn'nnn_
Cuts at: 0 203 1761
Size: 203 1558
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1192 1761
Size: 1192 569
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 628 1030 1404 1594 1761
Size: 42 586 402 374 190 167
Fragments arranged by size:
586 402 374 190 167 42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 367 781 1422 1761
Size: 367 414 641 339
Fragments arranged by size:
641 414 367 339
EcoO109I rG'GnC_Cy
Cuts at: 0 96 1516 1761
Size: 96 1420 245
Fragments arranged by size:
1420 245 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 882 1761
Size: 882 879
FspI TGC'GCA
Cuts at: 0 1219 1761
Size: 1219 542
HaeII r_GCGC'y
Cuts at: 0 169 1018 1057 1160 1761
Size: 169 849 39 103 601
Fragments arranged by size:
849 601 169 103 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 754 786 1471 1503 1761
Size: 577 32 145 32 685 32 258
Fragments arranged by size:
685 577 258 145 32 32 32
HincII GTy'rAC
Cuts at: 0 1116 1761
Size: 1116 645
KasI G'GCGC_C
Cuts at: 0 165 1053 1156 1761
Size: 165 888 103 605
Fragments arranged by size:
888 605 165 103
MluI A'CGCG_T
Cuts at: 0 767 1761
Size: 767 994
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 686 1761
Size: 482 204 1075
Fragments arranged by size:
1075 482 204
MscI TGG'CCA
Cuts at: 0 302 1198 1761
Size: 302 896 563
Fragments arranged by size:
896 563 302
MslI CAynn'nnrTG
Cuts at: 0 1300 1761
Size: 1300 461
MspA1I CmG'CkG
Cuts at: 0 162 279 557 566 876 972 1050 1230
Size: 162 117 278 9 310 96 78 180
Cuts at: 1230 1444 1508 1761
Size: 214 64 253
Fragments arranged by size:
310 278 253 214 180 162 117 96
78 64 9
NaeI GCC'GGC
Cuts at: 0 89 1406 1761
Size: 89 1317 355
Fragments arranged by size:
1317 355 89
NarI GG'CG_CC
Cuts at: 0 166 1054 1157 1761
Size: 166 888 103 604
Fragments arranged by size:
888 604 166 103
NgoMIV G'CCGG_C
Cuts at: 0 87 1404 1761
Size: 87 1317 357
Fragments arranged by size:
1317 357 87
PasI CC'CwG_GG
Cuts at: 0 340 1761
Size: 340 1421
PfoI T'CCnGG_A
Cuts at: 0 1535 1761
Size: 1535 226
PspOMI G'GGCC_C
Cuts at: 0 95 1516 1761
Size: 95 1421 245
Fragments arranged by size:
1421 245 95
PstI C_TGCA'G
Cuts at: 0 329 368 1761
Size: 329 39 1393
Fragments arranged by size:
1393 329 39
PvuII CAG'CTG
Cuts at: 0 162 1230 1761
Size: 162 1068 531
Fragments arranged by size:
1068 531 162
SacII CC_GC'GG
Cuts at: 0 567 1445 1761
Size: 567 878 316
Fragments arranged by size:
878 567 316
SbfI CC_TGCA'GG
Cuts at: 0 368 1761
Size: 368 1393
ScaI AGT'ACT
Cuts at: 0 25 1761
Size: 25 1736
SfcI C'TryA_G
Cuts at: 0 325 364 903 1761
Size: 325 39 539 858
Fragments arranged by size:
858 539 325 39
SfoI GGC'GCC
Cuts at: 0 167 1055 1158 1761
Size: 167 888 103 603
Fragments arranged by size:
888 603 167 103
SmaI CCC'GGG
Cuts at: 0 94 1761
Size: 94 1667
SmlI C'TyrA_G
Cuts at: 0 714 1330 1761
Size: 714 616 431
Fragments arranged by size:
714 616 431
SrfI GCCC'GGGC
Cuts at: 0 94 1761
Size: 94 1667
StuI AGG'CCT
Cuts at: 0 214 712 787 1761
Size: 214 498 75 974
Fragments arranged by size:
974 498 214 75
StyI C'CwwG_G
Cuts at: 0 114 641 1761
Size: 114 527 1120
Fragments arranged by size:
1120 527 114
TatI w'GTAC_w
Cuts at: 0 23 1100 1761
Size: 23 1077 661
Fragments arranged by size:
1077 661 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 629 661 871 903 1761
Size: 57 32 540 32 210 32 858
Fragments arranged by size:
858 540 210 57 32 32 32
XmaI C'CCGG_G
Cuts at: 0 92 1761
Size: 92 1669
ZraI GAC'GTC
Cuts at: 0 1176 1761
Size: 1176 585
Enzymes that do cut:
AatII AcuI AflIII AhdI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BclI BglI BlpI Bme1580I BmgBI
BmrI BpmI BpuEI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HincII
KasI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV PasI PfoI PspOMI PstI PvuII SacII SbfI
ScaI SfcI SfoI SmaI SmlI SrfI StuI StyI
TatI TstI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BfrBI BglII BmtI
BplI Bpu10I BsaI BsaBI BsaWI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrGI BstXI BstZ17I Bsu36I CspCI DraI
EcoICRI EcoRI EcoRV FseI FspAI HindIII HpaI KpnI
MfeI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SspI SwaI TaqII Tth111I XbaI XcmI XhoI XmnI