Restriction map for
pF1KB6116
(Linear) (Six-base) MAPSORT of: KIBB6116.seq Check: 381 from: 1 to: 1746
KIBB6116.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 459 1746
Size: 459 1287
AccI GT'mk_AC
Cuts at: 0 1691 1746
Size: 1691 55
Acc65I G'GTAC_C
Cuts at: 0 143 1746
Size: 143 1603
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1726 1746
Size: 1726 20
AflIII A'CryG_T
Cuts at: 0 1646 1746
Size: 1646 100
AleI CACnn'nnGTG
Cuts at: 0 262 1746
Size: 262 1484
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 499 1438 1746
Size: 499 939 308
Fragments arranged by size:
939 499 308
AlwNI CAG_nnn'CTG
Cuts at: 0 57 161 651 987 1746
Size: 57 104 490 336 759
Fragments arranged by size:
759 490 336 104 57
ApaI G_GGCC'C
Cuts at: 0 16 118 1272 1746
Size: 16 102 1154 474
Fragments arranged by size:
1154 474 102 16
ApaLI G'TGCA_C
Cuts at: 0 781 1746
Size: 781 965
AvaI C'yCGr_G
Cuts at: 0 167 970 1288 1400 1451 1746
Size: 167 803 318 112 51 295
Fragments arranged by size:
803 318 295 167 112 51
BanI G'GyrC_C
Cuts at: 0 143 287 547 562 898 994 1237 1746
Size: 143 144 260 15 336 96 243 509
Fragments arranged by size:
509 336 260 243 144 143 96 15
BanII G_rGCy'C
Cuts at: 0 16 118 398 824 1272 1401 1700 1745
Size: 16 102 280 426 448 129 299 45
Cuts at: 1745 1746
Size: 1
Fragments arranged by size:
448 426 299 280 129 102 45 16
1
BbeI G_GCGC'C
Cuts at: 0 291 1746
Size: 291 1455
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 1526 1746
Size: 464 1062 220
Fragments arranged by size:
1062 464 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 552 586 888 922 1746
Size: 552 34 302 34 824
Fragments arranged by size:
824 552 302 34 34
BclI T'GATC_A
Cuts at: 0 629 1746
Size: 629 1117
BglI GCCn_nnn'nGGC
Cuts at: 0 106 320 1331 1746
Size: 106 214 1011 415
Fragments arranged by size:
1011 415 214 106
Bme1580I G_kGCm'C
Cuts at: 0 16 118 785 1272 1370 1746
Size: 16 102 667 487 98 376
Fragments arranged by size:
667 487 376 102 98 16
BmgBI CAC'GTC
Cuts at: 0 765 780 1746
Size: 765 15 966
Fragments arranged by size:
966 765 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1581 1746
Size: 1581 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 838 1082 1746
Size: 689 149 244 664
Fragments arranged by size:
689 664 244 149
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1568 1684 1746
Size: 1568 116 62
Fragments arranged by size:
1568 116 62
BsaI GGTCTCn'nnnn_
Cuts at: 0 1162 1174 1746
Size: 1162 12 572
Fragments arranged by size:
1162 572 12
BsaBI GATnn'nnATC
Cuts at: 0 688 1746
Size: 688 1058
BsaHI Gr'CG_yC
Cuts at: 0 288 345 1605 1746
Size: 288 57 1260 141
Fragments arranged by size:
1260 288 141 57
BsaWI w'CCGG_w
Cuts at: 0 405 1554 1746
Size: 405 1149 192
Fragments arranged by size:
1149 405 192
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 868 898 1432 1462 1746
Size: 550 30 288 30 534 30 284
Fragments arranged by size:
550 534 288 284 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 1573 1738 1746
Size: 22 1551 165 8
Fragments arranged by size:
1551 165 22 8
BseYI C'CCAG_C
Cuts at: 0 19 90 654 1241 1746
Size: 19 71 564 587 505
Fragments arranged by size:
587 564 505 71 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 91 196 487 1746
Size: 91 105 291 1259
Fragments arranged by size:
1259 291 105 91
BsiEI CG_ry'CG
Cuts at: 0 110 1746
Size: 110 1636
BsiHKAI G_wGCw'C
Cuts at: 0 478 785 824 1700 1745 1746
Size: 478 307 39 876 45 1
Fragments arranged by size:
876 478 307 45 39 1
BsmBI CGTCTCn'nnnn_
Cuts at: 0 805 1746
Size: 805 941
Bsp1286I G_dGCh'C
Cuts at: 0 16 118 398 478 785 824 1272 1370
Size: 16 102 280 80 307 39 448 98
Cuts at: 1370 1401 1700 1745 1746
Size: 31 299 45 1
Fragments arranged by size:
448 307 299 280 102 98 80 45
39 31 16 1
BspEI T'CCGG_A
Cuts at: 0 1554 1746
Size: 1554 192
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 459 1180 1746
Size: 459 721 566
Fragments arranged by size:
721 566 459
BsrBI CCG'CTC
Cuts at: 0 339 1728 1746
Size: 339 1389 18
Fragments arranged by size:
1389 339 18
BsrDI GCAATG_nn'
Cuts at: 0 478 1182 1746
Size: 478 704 564
Fragments arranged by size:
704 564 478
BssSI C'ACGA_G
Cuts at: 0 1735 1746
Size: 1735 11
BstAPI GCAn_nnn'nTGC
Cuts at: 0 57 1746
Size: 57 1689
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 544 880 1746
Size: 276 268 336 866
Fragments arranged by size:
866 336 276 268
BstYI r'GATC_y
Cuts at: 0 1070 1746
Size: 1070 676
BtgI C'CryG_G
Cuts at: 0 8 77 1383 1746
Size: 8 69 1306 363
Fragments arranged by size:
1306 363 69 8
BtsI GCAGTG_nn'
Cuts at: 0 495 1422 1746
Size: 495 927 324
Fragments arranged by size:
927 495 324
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1173 1208 1746
Size: 1173 35 538
Fragments arranged by size:
1173 538 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 610 1746
Size: 610 1136
EaeI y'GGCC_r
Cuts at: 0 107 266 1159 1746
Size: 107 159 893 587
Fragments arranged by size:
893 587 159 107
EagI C'GGCC_G
Cuts at: 0 107 1746
Size: 107 1639
EarI CTCTTCn'nnn_
Cuts at: 0 910 1746
Size: 910 836
EcoICRI GAG'CTC
Cuts at: 0 822 1698 1743 1746
Size: 822 876 45 3
Fragments arranged by size:
876 822 45 3
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 838 1082 1726 1746
Size: 689 149 244 644 20
Fragments arranged by size:
689 644 244 149 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1534 1746
Size: 1534 212
EcoO109I rG'GnC_Cy
Cuts at: 0 13 114 115 158 1268 1269 1497 1548
Size: 13 101 1 43 1110 1 228 51
Cuts at: 1548 1623 1746
Size: 75 123
Fragments arranged by size:
1110 228 123 101 75 51 43 13
1 1
HaeII r_GCGC'y
Cuts at: 0 291 1746
Size: 291 1455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 1055 1087 1432 1464 1746
Size: 550 32 473 32 345 32 282
Fragments arranged by size:
550 473 345 282 32 32 32
HincII GTy'rAC
Cuts at: 0 333 1692 1746
Size: 333 1359 54
Fragments arranged by size:
1359 333 54
HindIII A'AGCT_T
Cuts at: 0 1189 1746
Size: 1189 557
KasI G'GCGC_C
Cuts at: 0 287 1746
Size: 287 1459
KpnI G_GTAC'C
Cuts at: 0 147 1746
Size: 147 1599
MscI TGG'CCA
Cuts at: 0 268 1746
Size: 268 1478
MslI CAynn'nnrTG
Cuts at: 0 262 274 301 322 542 878 1339 1746
Size: 262 12 27 21 220 336 461 407
Fragments arranged by size:
461 407 336 262 220 27 21 12
MspA1I CmG'CkG
Cuts at: 0 10 1245 1746
Size: 10 1235 501
Fragments arranged by size:
1235 501 10
NarI GG'CG_CC
Cuts at: 0 288 1746
Size: 288 1458
NspI r_CATG'y
Cuts at: 0 327 663 1338 1650 1746
Size: 327 336 675 312 96
Fragments arranged by size:
675 336 327 312 96
PasI CC'CwG_GG
Cuts at: 0 155 1746
Size: 155 1591
PciI A'CATG_T
Cuts at: 0 1646 1746
Size: 1646 100
PfoI T'CCnGG_A
Cuts at: 0 512 666 1746
Size: 512 154 1080
Fragments arranged by size:
1080 512 154
PpuMI rG'GwC_Cy
Cuts at: 0 1497 1548 1623 1746
Size: 1497 51 75 123
Fragments arranged by size:
1497 123 75 51
PspOMI G'GGCC_C
Cuts at: 0 12 114 1268 1746
Size: 12 102 1154 478
Fragments arranged by size:
1154 478 102 12
PstI C_TGCA'G
Cuts at: 0 53 992 1331 1746
Size: 53 939 339 415
Fragments arranged by size:
939 415 339 53
PvuII CAG'CTG
Cuts at: 0 1245 1746
Size: 1245 501
SacI G_AGCT'C
Cuts at: 0 824 1700 1745 1746
Size: 824 876 45 1
Fragments arranged by size:
876 824 45 1
SacII CC_GC'GG
Cuts at: 0 11 1746
Size: 11 1735
SalI G'TCGA_C
Cuts at: 0 1690 1746
Size: 1690 56
SbfI CC_TGCA'GG
Cuts at: 0 1331 1746
Size: 1331 415
SfcI C'TryA_G
Cuts at: 0 49 582 988 1327 1746
Size: 49 533 406 339 419
Fragments arranged by size:
533 419 406 339 49
SfoI GGC'GCC
Cuts at: 0 289 1746
Size: 289 1457
SmaI CCC'GGG
Cuts at: 0 169 972 1402 1453 1746
Size: 169 803 430 51 293
Fragments arranged by size:
803 430 293 169 51
SmlI C'TyrA_G
Cuts at: 0 1583 1699 1746
Size: 1583 116 47
Fragments arranged by size:
1583 116 47
StuI AGG'CCT
Cuts at: 0 314 748 1313 1746
Size: 314 434 565 433
Fragments arranged by size:
565 434 433 314
StyI C'CwwG_G
Cuts at: 0 260 1746
Size: 260 1486
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 323 1255 1746
Size: 268 55 932 491
Fragments arranged by size:
932 491 268 55
Tth111I GACn'n_nGTC
Cuts at: 0 526 799 1152 1746
Size: 526 273 353 594
Fragments arranged by size:
594 526 353 273
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 370 1390 1746
Size: 370 1020 356
Fragments arranged by size:
1020 370 356
XmaI C'CCGG_G
Cuts at: 0 167 970 1400 1451 1746
Size: 167 803 430 51 295
Fragments arranged by size:
803 430 295 167 51
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AleI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI
BclI BglI Bme1580I BmgBI BplI BpmI BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BssSI
BstAPI BstXI BstYI BtgI BtsI CspCI DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII KasI KpnI MscI MslI MspA1I NarI
NspI PasI PciI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SalI SbfI SfcI SfoI SmaI SmlI
StuI StyI TaqII Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BfrBI BglII BlpI BmrI BmtI Bpu10I BsaAI BsiWI
BsmI BspHI BsrFI BsrGI BssHII BstBI BstEII BstZ17I
Bsu36I BtgZI ClaI DraI DraIII EciI EcoRI EcoRV
FalI FseI FspI FspAI HpaI MfeI MluI MmeI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI XbaI XhoI XmnI ZraI