Restriction map for
pF1KB6115
(Linear) (Six-base) MAPSORT of: KIBB6115.seq Check: 3144 from: 1 to: 1860
KIBB6115.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 642 1860
Size: 642 1218
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 741 1611 1860
Size: 741 870 249
Fragments arranged by size:
870 741 249
AleI CACnn'nnGTG
Cuts at: 0 949 1860
Size: 949 911
AlwNI CAG_nnn'CTG
Cuts at: 0 764 1320 1586 1860
Size: 764 556 266 274
Fragments arranged by size:
764 556 274 266
ApaI G_GGCC'C
Cuts at: 0 76 1860
Size: 76 1784
AvaI C'yCGr_G
Cuts at: 0 603 1789 1860
Size: 603 1186 71
Fragments arranged by size:
1186 603 71
BamHI G'GATC_C
Cuts at: 0 530 1860
Size: 530 1330
BanI G'GyrC_C
Cuts at: 0 286 1713 1860
Size: 286 1427 147
Fragments arranged by size:
1427 286 147
BanII G_rGCy'C
Cuts at: 0 62 76 92 160 524 1301 1340 1860
Size: 62 14 16 68 364 777 39 520
Fragments arranged by size:
777 520 364 68 62 39 16 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 784 1083 1221 1404 1860
Size: 26 758 299 138 183 456
Fragments arranged by size:
758 456 299 183 138 26
BbvCI CC'TCA_GC
Cuts at: 0 1212 1860
Size: 1212 648
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 180 214 1860
Size: 180 34 1646
Fragments arranged by size:
1646 180 34
BclI T'GATC_A
Cuts at: 0 1289 1860
Size: 1289 571
BglII A'GATC_T
Cuts at: 0 398 1860
Size: 398 1462
Bme1580I G_kGCm'C
Cuts at: 0 76 1133 1718 1860
Size: 76 1057 585 142
Fragments arranged by size:
1057 585 142 76
BmgBI CAC'GTC
Cuts at: 0 1143 1445 1860
Size: 1143 302 415
Fragments arranged by size:
1143 415 302
BmrI ACTGGGnnnn_n'
Cuts at: 0 1674 1860
Size: 1674 186
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 1860
Size: 139 1721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 884 981 1860
Size: 573 311 97 879
Fragments arranged by size:
879 573 311 97
Bpu10I CC'TnA_GC
Cuts at: 0 53 892 1212 1860
Size: 53 839 320 648
Fragments arranged by size:
839 648 320 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1837 1860
Size: 1837 23
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 1860
Size: 175 1685
BsaAI yAC'GTr
Cuts at: 0 822 1860
Size: 822 1038
BsaBI GATnn'nnATC
Cuts at: 0 575 1171 1860
Size: 575 596 689
Fragments arranged by size:
689 596 575
BsaHI Gr'CG_yC
Cuts at: 0 922 1860
Size: 922 938
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 495 1065 1860
Size: 495 570 795
Fragments arranged by size:
795 570 495
BseYI C'CCAG_C
Cuts at: 0 325 1546 1677 1682 1860
Size: 325 1221 131 5 178
Fragments arranged by size:
1221 325 178 131 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 475 649 1306 1860
Size: 139 336 174 657 554
Fragments arranged by size:
657 554 336 174 139
BsiHKAI G_wGCw'C
Cuts at: 0 92 524 1340 1466 1860
Size: 92 432 816 126 394
Fragments arranged by size:
816 432 394 126 92
Bsp1286I G_dGCh'C
Cuts at: 0 62 76 92 160 524 1133 1301 1340
Size: 62 14 16 68 364 609 168 39
Cuts at: 1340 1466 1718 1860
Size: 126 252 142
Fragments arranged by size:
609 364 252 168 142 126 68 62
39 16 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 314 642 1860
Size: 314 328 1218
Fragments arranged by size:
1218 328 314
BsrDI GCAATG_nn'
Cuts at: 0 320 1781 1860
Size: 320 1461 79
Fragments arranged by size:
1461 320 79
BsrFI r'CCGG_y
Cuts at: 0 908 1438 1860
Size: 908 530 422
Fragments arranged by size:
908 530 422
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1128 1860
Size: 1128 732
BstEII G'GTnAC_C
Cuts at: 0 852 1860
Size: 852 1008
BstXI CCAn_nnnn'nTGG
Cuts at: 0 69 682 1482 1677 1710 1860
Size: 69 613 800 195 33 150
Fragments arranged by size:
800 613 195 150 69 33
BstYI r'GATC_y
Cuts at: 0 398 530 1860
Size: 398 132 1330
Fragments arranged by size:
1330 398 132
Bsu36I CC'TnA_GG
Cuts at: 0 783 1860
Size: 783 1077
BtgI C'CryG_G
Cuts at: 0 20 38 68 148 161 515 681 706
Size: 20 18 30 80 13 354 166 25
Cuts at: 706 1223 1268 1703 1709 1860
Size: 517 45 435 6 151
Fragments arranged by size:
517 435 354 166 151 80 45 30
25 20 18 13 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 462 1176 1674 1860
Size: 462 714 498 186
Fragments arranged by size:
714 498 462 186
BtsI GCAGTG_nn'
Cuts at: 0 534 830 1860
Size: 534 296 1030
Fragments arranged by size:
1030 534 296
ClaI AT'CG_AT
Cuts at: 0 1304 1860
Size: 1304 556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 261 296 1860
Size: 261 35 1564
Fragments arranged by size:
1564 261 35
DraIII CAC_nnn'GTG
Cuts at: 0 1128 1860
Size: 1128 732
DrdI GACnn_nn'nnGTC
Cuts at: 0 1039 1860
Size: 1039 821
EaeI y'GGCC_r
Cuts at: 0 239 360 941 1145 1706 1860
Size: 239 121 581 204 561 154
Fragments arranged by size:
581 561 239 204 154 121
EarI CTCTTCn'nnn_
Cuts at: 0 316 505 1345 1354 1860
Size: 316 189 840 9 506
Fragments arranged by size:
840 506 316 189 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1831 1860
Size: 1831 29
EcoICRI GAG'CTC
Cuts at: 0 90 522 1338 1860
Size: 90 432 816 522
Fragments arranged by size:
816 522 432 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 573 741 884 981 1611 1860
Size: 573 168 143 97 630 249
Fragments arranged by size:
630 573 249 168 143 97
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 766 1860
Size: 100 666 1094
Fragments arranged by size:
1094 666 100
EcoO109I rG'GnC_Cy
Cuts at: 0 700 1754 1860
Size: 700 1054 106
Fragments arranged by size:
1054 700 106
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 219 1860
Size: 219 1641
HaeII r_GCGC'y
Cuts at: 0 490 1268 1860
Size: 490 778 592
Fragments arranged by size:
778 592 490
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1451 1483 1637 1669 1860
Size: 1451 32 154 32 191
Fragments arranged by size:
1451 191 154 32 32
HincII GTy'rAC
Cuts at: 0 900 1860
Size: 900 960
MscI TGG'CCA
Cuts at: 0 241 1147 1708 1860
Size: 241 906 561 152
Fragments arranged by size:
906 561 241 152
MslI CAynn'nnrTG
Cuts at: 0 581 949 1480 1708 1860
Size: 581 368 531 228 152
Fragments arranged by size:
581 531 368 228 152
MspA1I CmG'CkG
Cuts at: 0 150 385 708 1317 1542 1860
Size: 150 235 323 609 225 318
Fragments arranged by size:
609 323 318 235 225 150
NaeI GCC'GGC
Cuts at: 0 1440 1860
Size: 1440 420
NcoI C'CATG_G
Cuts at: 0 681 1268 1703 1709 1860
Size: 681 587 435 6 151
Fragments arranged by size:
681 587 435 151 6
NgoMIV G'CCGG_C
Cuts at: 0 1438 1860
Size: 1438 422
PasI CC'CwG_GG
Cuts at: 0 963 1251 1860
Size: 963 288 609
Fragments arranged by size:
963 609 288
PflMI CCAn_nnn'nTGG
Cuts at: 0 248 1860
Size: 248 1612
PfoI T'CCnGG_A
Cuts at: 0 547 1421 1860
Size: 547 874 439
Fragments arranged by size:
874 547 439
PpuMI rG'GwC_Cy
Cuts at: 0 1754 1860
Size: 1754 106
PshAI GACnn'nnGTC
Cuts at: 0 30 1860
Size: 30 1830
PspOMI G'GGCC_C
Cuts at: 0 72 1860
Size: 72 1788
PstI C_TGCA'G
Cuts at: 0 1744 1860
Size: 1744 116
PvuII CAG'CTG
Cuts at: 0 1317 1860
Size: 1317 543
SacI G_AGCT'C
Cuts at: 0 92 524 1340 1860
Size: 92 432 816 520
Fragments arranged by size:
816 520 432 92
SacII CC_GC'GG
Cuts at: 0 151 709 1860
Size: 151 558 1151
Fragments arranged by size:
1151 558 151
SapI GCTCTTCn'nnn_
Cuts at: 0 316 1345 1354 1860
Size: 316 1029 9 506
Fragments arranged by size:
1029 506 316 9
ScaI AGT'ACT
Cuts at: 0 646 1860
Size: 646 1214
SfcI C'TryA_G
Cuts at: 0 1026 1740 1860
Size: 1026 714 120
Fragments arranged by size:
1026 714 120
SmlI C'TyrA_G
Cuts at: 0 1852 1860
Size: 1852 8
StyI C'CwwG_G
Cuts at: 0 681 1148 1268 1703 1709 1860
Size: 681 467 120 435 6 151
Fragments arranged by size:
681 467 435 151 120 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 961 1261 1860
Size: 961 300 599
Fragments arranged by size:
961 599 300
TatI w'GTAC_w
Cuts at: 0 644 1553 1860
Size: 644 909 307
Fragments arranged by size:
909 644 307
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 679 819 1140 1275 1701 1860
Size: 679 140 321 135 426 159
Fragments arranged by size:
679 426 321 159 140 135
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI AvaI BamHI BanI
BanII BbsI BbvCI BcgI BclI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SacII
SapI ScaI SfcI SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbeI BciVI BfrBI BglI BlpI BmtI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstZ17I DraI EagI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI MmeI NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI