Restriction map for
pF1KB6113
(Linear) (Six-base) MAPSORT of: KIBB6113.seq Check: 4238 from: 1 to: 1860
KIBB6113.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 1760 1860
Size: 1760 100
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 503 533 1241 1860
Size: 172 331 30 708 619
Fragments arranged by size:
708 619 331 172 30
AfeI AGC'GCT
Cuts at: 0 301 1860
Size: 301 1559
AflIII A'CryG_T
Cuts at: 0 1755 1860
Size: 1755 105
AleI CACnn'nnGTG
Cuts at: 0 1093 1860
Size: 1093 767
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 381 413 1860
Size: 381 32 1447
Fragments arranged by size:
1447 381 32
ApoI r'AATT_y
Cuts at: 0 1555 1860
Size: 1555 305
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 988 1021 1698 1731 1860
Size: 988 33 677 33 129
Fragments arranged by size:
988 677 129 33 33
BanI G'GyrC_C
Cuts at: 0 348 1549 1860
Size: 348 1201 311
Fragments arranged by size:
1201 348 311
BanII G_rGCy'C
Cuts at: 0 170 1088 1860
Size: 170 918 772
Fragments arranged by size:
918 772 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 491 1712 1860
Size: 491 1221 148
Fragments arranged by size:
1221 491 148
BciVI GTATCCnnnnn_n'
Cuts at: 0 1016 1860
Size: 1016 844
BclI T'GATC_A
Cuts at: 0 1527 1860
Size: 1527 333
BglI GCCn_nnn'nGGC
Cuts at: 0 112 1860
Size: 112 1748
Bme1580I G_kGCm'C
Cuts at: 0 432 1860
Size: 432 1428
BmgBI CAC'GTC
Cuts at: 0 1758 1860
Size: 1758 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 1030 1860
Size: 1030 830
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1605 1860
Size: 1605 255
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 263 427 1297 1800 1860
Size: 263 164 870 503 60
Fragments arranged by size:
870 503 263 164 60
Bpu10I CC'TnA_GC
Cuts at: 0 905 1860
Size: 905 955
BsaI GGTCTCn'nnnn_
Cuts at: 0 1363 1785 1860
Size: 1363 422 75
Fragments arranged by size:
1363 422 75
BsaWI w'CCGG_w
Cuts at: 0 1001 1717 1860
Size: 1001 716 143
Fragments arranged by size:
1001 716 143
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 381 411 974 1004 1860
Size: 381 30 563 30 856
Fragments arranged by size:
856 563 381 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 995 1228 1860
Size: 995 233 632
Fragments arranged by size:
995 632 233
BseYI C'CCAG_C
Cuts at: 0 302 1346 1377 1860
Size: 302 1044 31 483
Fragments arranged by size:
1044 483 302 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1860
Size: 1276 584
BsiHKAI G_wGCw'C
Cuts at: 0 170 1088 1319 1860
Size: 170 918 231 541
Fragments arranged by size:
918 541 231 170
BsmI GAATG_Cn'
Cuts at: 0 456 1860
Size: 456 1404
Bsp1286I G_dGCh'C
Cuts at: 0 170 432 1088 1319 1860
Size: 170 262 656 231 541
Fragments arranged by size:
656 541 262 231 170
BsrDI GCAATG_nn'
Cuts at: 0 527 1860
Size: 527 1333
BsrFI r'CCGG_y
Cuts at: 0 293 1860
Size: 293 1567
BsrGI T'GTAC_A
Cuts at: 0 1730 1860
Size: 1730 130
BstAPI GCAn_nnn'nTGC
Cuts at: 0 218 1860
Size: 218 1642
BstXI CCAn_nnnn'nTGG
Cuts at: 0 976 1296 1860
Size: 976 320 564
Fragments arranged by size:
976 564 320
Bsu36I CC'TnA_GG
Cuts at: 0 560 1860
Size: 560 1300
BtsI GCAGTG_nn'
Cuts at: 0 571 1116 1776 1860
Size: 571 545 660 84
Fragments arranged by size:
660 571 545 84
DraI TTT'AAA
Cuts at: 0 78 1860
Size: 78 1782
DrdI GACnn_nn'nnGTC
Cuts at: 0 1826 1860
Size: 1826 34
EaeI y'GGCC_r
Cuts at: 0 1804 1860
Size: 1804 56
EarI CTCTTCn'nnn_
Cuts at: 0 473 581 956 1193 1415 1860
Size: 473 108 375 237 222 445
Fragments arranged by size:
473 445 375 237 222 108
EcoICRI GAG'CTC
Cuts at: 0 168 1086 1860
Size: 168 918 774
Fragments arranged by size:
918 774 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 263 427 503 533 1241 1297 1800
Size: 172 91 164 76 30 708 56 503
Cuts at: 1800 1860
Size: 60
Fragments arranged by size:
708 503 172 164 91 76 60 56
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1279 1420 1860
Size: 1279 141 440
Fragments arranged by size:
1279 440 141
EcoO109I rG'GnC_Cy
Cuts at: 0 1284 1860
Size: 1284 576
HaeII r_GCGC'y
Cuts at: 0 303 1860
Size: 303 1557
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 1758 1790 1860
Size: 1609 32 117 32 70
Fragments arranged by size:
1609 117 70 32 32
HincII GTy'rAC
Cuts at: 0 98 1831 1860
Size: 98 1733 29
Fragments arranged by size:
1733 98 29
HindIII A'AGCT_T
Cuts at: 0 412 1860
Size: 412 1448
HpaI GTT'AAC
Cuts at: 0 98 1860
Size: 98 1762
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1860
Size: 466 1394
MslI CAynn'nnrTG
Cuts at: 0 1093 1489 1860
Size: 1093 396 371
Fragments arranged by size:
1093 396 371
MspA1I CmG'CkG
Cuts at: 0 405 1260 1350 1860
Size: 405 855 90 510
Fragments arranged by size:
855 510 405 90
NaeI GCC'GGC
Cuts at: 0 295 1860
Size: 295 1565
NgoMIV G'CCGG_C
Cuts at: 0 293 1860
Size: 293 1567
NspI r_CATG'y
Cuts at: 0 1386 1430 1860
Size: 1386 44 430
Fragments arranged by size:
1386 430 44
PfoI T'CCnGG_A
Cuts at: 0 17 632 799 1860
Size: 17 615 167 1061
Fragments arranged by size:
1061 615 167 17
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 381 413 1860
Size: 381 32 1447
Fragments arranged by size:
1447 381 32
PstI C_TGCA'G
Cuts at: 0 810 1368 1860
Size: 810 558 492
Fragments arranged by size:
810 558 492
PvuII CAG'CTG
Cuts at: 0 405 1260 1860
Size: 405 855 600
Fragments arranged by size:
855 600 405
SacI G_AGCT'C
Cuts at: 0 170 1088 1860
Size: 170 918 772
Fragments arranged by size:
918 772 170
ScaI AGT'ACT
Cuts at: 0 592 1860
Size: 592 1268
SfcI C'TryA_G
Cuts at: 0 806 1364 1602 1860
Size: 806 558 238 258
Fragments arranged by size:
806 558 258 238
StuI AGG'CCT
Cuts at: 0 986 1860
Size: 986 874
StyI C'CwwG_G
Cuts at: 0 836 1860
Size: 836 1024
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 294 1056 1360 1860
Size: 294 762 304 500
Fragments arranged by size:
762 500 304 294
TatI w'GTAC_w
Cuts at: 0 590 1532 1730 1860
Size: 590 942 198 130
Fragments arranged by size:
942 590 198 130
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 974 1006 1860
Size: 370 32 572 32 854
Fragments arranged by size:
854 572 370 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 640 1461 1860
Size: 108 532 821 399
Fragments arranged by size:
821 532 399 108
XmnI GAAnn'nnTTC
Cuts at: 0 60 752 1860
Size: 60 692 1108
Fragments arranged by size:
1108 692 60
Enzymes that do cut:
AccI AcuI AfeI AflIII AleI AloI ApoI BaeI
BanI BanII BbsI BciVI BclI BglI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BsrGI
BstAPI BstXI Bsu36I BtsI DraI DrdI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII
HpaI MmeI MslI MspA1I NaeI NgoMIV NspI PfoI
PpiI PstI PvuII SacI ScaI SfcI StuI StyI
TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BbeI BbvCI BcgI BfrBI BglII BlpI BmtI
BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BssHII BssSI BstBI BstEII BstYI
BstZ17I BtgI BtgZI ClaI CspCI DraIII EagI EciI
EcoRI EcoRV FalI FseI FspI FspAI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmaI ZraI