Restriction map for
pF1KB6102
(Linear) (Six-base) MAPSORT of: KIBB6102.seq Check: 6687 from: 1 to: 1842
KIBB6102.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1815 1842
Size: 1815 27
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 886 1842
Size: 886 956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 857 1842
Size: 857 985
AlwNI CAG_nnn'CTG
Cuts at: 0 639 1589 1668 1842
Size: 639 950 79 174
Fragments arranged by size:
950 639 174 79
ApaLI G'TGCA_C
Cuts at: 0 715 1842
Size: 715 1127
ApoI r'AATT_y
Cuts at: 0 499 1376 1842
Size: 499 877 466
Fragments arranged by size:
877 499 466
AvaI C'yCGr_G
Cuts at: 0 608 868 1842
Size: 608 260 974
Fragments arranged by size:
974 608 260
BanI G'GyrC_C
Cuts at: 0 108 274 1643 1842
Size: 108 166 1369 199
Fragments arranged by size:
1369 199 166 108
BanII G_rGCy'C
Cuts at: 0 1684 1726 1842
Size: 1684 42 116
Fragments arranged by size:
1684 116 42
BbvCI CC'TCA_GC
Cuts at: 0 1216 1412 1842
Size: 1216 196 430
Fragments arranged by size:
1216 430 196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 589 623 1772 1806 1842
Size: 589 34 1149 34 36
Fragments arranged by size:
1149 589 36 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1315 1842
Size: 1315 527
BglI GCCn_nnn'nGGC
Cuts at: 0 1688 1842
Size: 1688 154
BlpI GC'TnA_GC
Cuts at: 0 644 1842
Size: 644 1198
Bme1580I G_kGCm'C
Cuts at: 0 279 719 857 1757 1842
Size: 279 440 138 900 85
Fragments arranged by size:
900 440 279 138 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 627 731 1644 1842
Size: 627 104 913 198
Fragments arranged by size:
913 627 198 104
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1257 1842
Size: 1257 585
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 1227 1842
Size: 218 1009 615
Fragments arranged by size:
1009 615 218
Bpu10I CC'TnA_GC
Cuts at: 0 788 1216 1412 1842
Size: 788 428 196 430
Fragments arranged by size:
788 430 428 196
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 1842
Size: 547 1295
BsaI GGTCTCn'nnnn_
Cuts at: 0 1245 1842
Size: 1245 597
BsaAI yAC'GTr
Cuts at: 0 1326 1842
Size: 1326 516
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 768 798 1842
Size: 768 30 1044
Fragments arranged by size:
1044 768 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 248 306 762 818 1546 1716 1842
Size: 248 58 456 56 728 170 126
Fragments arranged by size:
728 456 248 170 126 58 56
BseYI C'CCAG_C
Cuts at: 0 79 1756 1761 1842
Size: 79 1677 5 81
Fragments arranged by size:
1677 81 79 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 583 1286 1842
Size: 314 269 703 556
Fragments arranged by size:
703 556 314 269
BsiEI CG_ry'CG
Cuts at: 0 1702 1842
Size: 1702 140
BsiHKAI G_wGCw'C
Cuts at: 0 369 719 1458 1842
Size: 369 350 739 384
Fragments arranged by size:
739 384 369 350
Bsp1286I G_dGCh'C
Cuts at: 0 279 369 719 857 1458 1684 1726 1757
Size: 279 90 350 138 601 226 42 31
Cuts at: 1757 1842
Size: 85
Fragments arranged by size:
601 350 279 226 138 90 85 42
31
BspHI T'CATG_A
Cuts at: 0 860 1842
Size: 860 982
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 581 1676 1815 1842
Size: 581 1095 139 27
Fragments arranged by size:
1095 581 139 27
BsrBI CCG'CTC
Cuts at: 0 1641 1842
Size: 1641 201
BsrDI GCAATG_nn'
Cuts at: 0 246 309 426 674 1287 1842
Size: 246 63 117 248 613 555
Fragments arranged by size:
613 555 248 246 117 63
BsrFI r'CCGG_y
Cuts at: 0 1697 1842
Size: 1697 145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 685 1504 1589 1842
Size: 685 819 85 253
Fragments arranged by size:
819 685 253 85
BstXI CCAn_nnnn'nTGG
Cuts at: 0 79 1000 1842
Size: 79 921 842
Fragments arranged by size:
921 842 79
BstYI r'GATC_y
Cuts at: 0 154 1842
Size: 154 1688
Bsu36I CC'TnA_GG
Cuts at: 0 45 1842
Size: 45 1797
BtgI C'CryG_G
Cuts at: 0 1538 1686 1842
Size: 1538 148 156
Fragments arranged by size:
1538 156 148
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 130 665 1775 1842
Size: 130 535 1110 67
Fragments arranged by size:
1110 535 130 67
BtsI GCAGTG_nn'
Cuts at: 0 600 1590 1842
Size: 600 990 252
Fragments arranged by size:
990 600 252
DrdI GACnn_nn'nnGTC
Cuts at: 0 995 1842
Size: 995 847
EaeI y'GGCC_r
Cuts at: 0 18 614 1560 1674 1689 1699 1842
Size: 18 596 946 114 15 10 143
Fragments arranged by size:
946 596 143 114 18 15 10
EagI C'GGCC_G
Cuts at: 0 1699 1842
Size: 1699 143
EarI CTCTTCn'nnn_
Cuts at: 0 724 749 905 1842
Size: 724 25 156 937
Fragments arranged by size:
937 724 156 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1728 1842
Size: 1728 114
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 886 1227 1842
Size: 218 668 341 615
Fragments arranged by size:
668 615 341 218
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1731 1842
Size: 1731 111
EcoRI G'AATT_C
Cuts at: 0 499 1842
Size: 499 1343
FseI GG_CCGG'CC
Cuts at: 0 1701 1842
Size: 1701 141
HaeII r_GCGC'y
Cuts at: 0 1131 1152 1842
Size: 1131 21 690
Fragments arranged by size:
1131 690 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 723 755 1762 1794 1842
Size: 723 32 1007 32 48
Fragments arranged by size:
1007 723 48 32 32
MfeI C'AATT_G
Cuts at: 0 346 491 779 1842
Size: 346 145 288 1063
Fragments arranged by size:
1063 346 288 145
MscI TGG'CCA
Cuts at: 0 20 1676 1842
Size: 20 1656 166
Fragments arranged by size:
1656 166 20
MslI CAynn'nnrTG
Cuts at: 0 712 722 1842
Size: 712 10 1120
Fragments arranged by size:
1120 712 10
MspA1I CmG'CkG
Cuts at: 0 588 1704 1842
Size: 588 1116 138
Fragments arranged by size:
1116 588 138
NaeI GCC'GGC
Cuts at: 0 1699 1842
Size: 1699 143
NgoMIV G'CCGG_C
Cuts at: 0 1697 1842
Size: 1697 145
NspI r_CATG'y
Cuts at: 0 1583 1842
Size: 1583 259
PasI CC'CwG_GG
Cuts at: 0 1258 1842
Size: 1258 584
PflMI CCAn_nnn'nTGG
Cuts at: 0 639 1842
Size: 639 1203
PfoI T'CCnGG_A
Cuts at: 0 195 1842
Size: 195 1647
PstI C_TGCA'G
Cuts at: 0 587 1750 1842
Size: 587 1163 92
Fragments arranged by size:
1163 587 92
SfcI C'TryA_G
Cuts at: 0 583 1712 1746 1842
Size: 583 1129 34 96
Fragments arranged by size:
1129 583 96 34
SmaI CCC'GGG
Cuts at: 0 870 1842
Size: 870 972
SmlI C'TyrA_G
Cuts at: 0 562 1842
Size: 562 1280
SnaBI TAC'GTA
Cuts at: 0 1326 1842
Size: 1326 516
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 219 1246 1842
Size: 219 1027 596
Fragments arranged by size:
1027 596 219
TatI w'GTAC_w
Cuts at: 0 1395 1842
Size: 1395 447
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 116 148 516 548 768 800 1842
Size: 116 32 368 32 220 32 1042
Fragments arranged by size:
1042 368 220 116 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1654 1842
Size: 1654 188
XmaI C'CCGG_G
Cuts at: 0 868 1842
Size: 868 974
XmnI GAAnn'nnTTC
Cuts at: 0 656 1842
Size: 656 1186
Enzymes that do cut:
AarI AcuI AlfI AlwNI ApaLI ApoI AvaI BanI
BanII BbvCI BcgI BciVI BglI BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI FseI HaeII Hin4I MfeI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PflMI PfoI PstI SfcI
SmaI SmlI SnaBI TaqII TatI TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BclI BfrBI BglII BmgBI
BmtI BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI
BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII EcoICRI EcoO109I EcoRV FalI FspI FspAI
HincII HindIII HpaI KasI KpnI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SpeI SphI SrfI SspI StuI StyI SwaI Tth111I
XbaI XhoI ZraI