Restriction map for
pF1KB5988
(Linear) (Six-base) MAPSORT of: KIBB5988.seq Check: 1830 from: 1 to: 1674
KIBB5988.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AccI GT'mk_AC
Cuts at: 0 660 914 1554 1674
Size: 660 254 640 120
Fragments arranged by size:
660 640 254 120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 136 548 1674
Size: 52 84 412 1126
Fragments arranged by size:
1126 412 84 52
AflIII A'CryG_T
Cuts at: 0 957 1359 1674
Size: 957 402 315
Fragments arranged by size:
957 402 315
AlwNI CAG_nnn'CTG
Cuts at: 0 1159 1674
Size: 1159 515
ApoI r'AATT_y
Cuts at: 0 245 876 1078 1674
Size: 245 631 202 596
Fragments arranged by size:
631 596 245 202
AseI AT'TA_AT
Cuts at: 0 1399 1674
Size: 1399 275
BamHI G'GATC_C
Cuts at: 0 824 1674
Size: 824 850
BanII G_rGCy'C
Cuts at: 0 336 719 1674
Size: 336 383 955
Fragments arranged by size:
955 383 336
BbsI GAAGACnn'nnnn_
Cuts at: 0 176 1674
Size: 176 1498
BciVI GTATCCnnnnn_n'
Cuts at: 0 1605 1674
Size: 1605 69
BglI GCCn_nnn'nGGC
Cuts at: 0 1636 1674
Size: 1636 38
BglII A'GATC_T
Cuts at: 0 732 1434 1674
Size: 732 702 240
Fragments arranged by size:
732 702 240
BlpI GC'TnA_GC
Cuts at: 0 374 1674
Size: 374 1300
Bme1580I G_kGCm'C
Cuts at: 0 1005 1423 1674
Size: 1005 418 251
Fragments arranged by size:
1005 418 251
BmrI ACTGGGnnnn_n'
Cuts at: 0 427 1674
Size: 427 1247
BmtI G_CTAG'C
Cuts at: 0 1480 1674
Size: 1480 194
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1511 1674
Size: 1511 163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 878 1674
Size: 878 796
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1674
Size: 508 1166
BseYI C'CCAG_C
Cuts at: 0 1054 1631 1674
Size: 1054 577 43
Fragments arranged by size:
1054 577 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 304 1333 1674
Size: 304 1029 341
Fragments arranged by size:
1029 341 304
BsiHKAI G_wGCw'C
Cuts at: 0 719 1497 1536 1674
Size: 719 778 39 138
Fragments arranged by size:
778 719 138 39
Bsp1286I G_dGCh'C
Cuts at: 0 336 719 1005 1423 1497 1536 1674
Size: 336 383 286 418 74 39 138
Fragments arranged by size:
418 383 336 286 138 74 39
BspHI T'CATG_A
Cuts at: 0 1611 1674
Size: 1611 63
BsrDI GCAATG_nn'
Cuts at: 0 521 1674
Size: 521 1153
BsrGI T'GTAC_A
Cuts at: 0 1455 1674
Size: 1455 219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 428 880 1000 1159 1674
Size: 428 452 120 159 515
Fragments arranged by size:
515 452 428 159 120
BstXI CCAn_nnnn'nTGG
Cuts at: 0 142 1674
Size: 142 1532
BstYI r'GATC_y
Cuts at: 0 732 824 1434 1543 1674
Size: 732 92 610 109 131
Fragments arranged by size:
732 610 131 109 92
BtgI C'CryG_G
Cuts at: 0 1343 1517 1674
Size: 1343 174 157
Fragments arranged by size:
1343 174 157
BtsI GCAGTG_nn'
Cuts at: 0 426 1421 1674
Size: 426 995 253
Fragments arranged by size:
995 426 253
DraIII CAC_nnn'GTG
Cuts at: 0 1000 1674
Size: 1000 674
DrdI GACnn_nn'nnGTC
Cuts at: 0 180 1674
Size: 180 1494
EaeI y'GGCC_r
Cuts at: 0 1016 1674
Size: 1016 658
EarI CTCTTCn'nnn_
Cuts at: 0 524 550 1674
Size: 524 26 1124
Fragments arranged by size:
1124 524 26
EcoICRI GAG'CTC
Cuts at: 0 717 1674
Size: 717 957
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 136 548 878 1674
Size: 52 84 412 330 796
Fragments arranged by size:
796 412 330 84 52
EcoRI G'AATT_C
Cuts at: 0 245 1674
Size: 245 1429
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 334 394 1674
Size: 334 60 1280
Fragments arranged by size:
1280 334 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1518 1550 1579 1611 1674
Size: 1518 32 29 32 63
Fragments arranged by size:
1518 63 32 32 29
HincII GTy'rAC
Cuts at: 0 48 173 1674
Size: 48 125 1501
Fragments arranged by size:
1501 125 48
MfeI C'AATT_G
Cuts at: 0 869 1168 1674
Size: 869 299 506
Fragments arranged by size:
869 506 299
MscI TGG'CCA
Cuts at: 0 1018 1674
Size: 1018 656
MslI CAynn'nnrTG
Cuts at: 0 1232 1674
Size: 1232 442
MspA1I CmG'CkG
Cuts at: 0 43 156 288 633 1054 1674
Size: 43 113 132 345 421 620
Fragments arranged by size:
620 421 345 132 113 43
NcoI C'CATG_G
Cuts at: 0 1343 1517 1674
Size: 1343 174 157
Fragments arranged by size:
1343 174 157
NdeI CA'TA_TG
Cuts at: 0 346 908 1674
Size: 346 562 766
Fragments arranged by size:
766 562 346
NheI G'CTAG_C
Cuts at: 0 1476 1674
Size: 1476 198
NspI r_CATG'y
Cuts at: 0 961 991 1363 1674
Size: 961 30 372 311
Fragments arranged by size:
961 372 311 30
PciI A'CATG_T
Cuts at: 0 957 1359 1674
Size: 957 402 315
Fragments arranged by size:
957 402 315
PshAI GACnn'nnGTC
Cuts at: 0 1260 1674
Size: 1260 414
PsiI TTA'TAA
Cuts at: 0 1030 1674
Size: 1030 644
PvuII CAG'CTG
Cuts at: 0 43 156 288 633 1054 1674
Size: 43 113 132 345 421 620
Fragments arranged by size:
620 421 345 132 113 43
SacI G_AGCT'C
Cuts at: 0 719 1674
Size: 719 955
SapI GCTCTTCn'nnn_
Cuts at: 0 524 1674
Size: 524 1150
SfcI C'TryA_G
Cuts at: 0 628 847 1674
Size: 628 219 827
Fragments arranged by size:
827 628 219
SmlI C'TyrA_G
Cuts at: 0 487 1674
Size: 487 1187
SpeI A'CTAG_T
Cuts at: 0 1351 1674
Size: 1351 323
SphI G_CATG'C
Cuts at: 0 991 1674
Size: 991 683
StuI AGG'CCT
Cuts at: 0 647 1639 1674
Size: 647 992 35
Fragments arranged by size:
992 647 35
StyI C'CwwG_G
Cuts at: 0 1343 1517 1674
Size: 1343 174 157
Fragments arranged by size:
1343 174 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 379 1674
Size: 379 1295
TatI w'GTAC_w
Cuts at: 0 514 981 1455 1566 1674
Size: 514 467 474 111 108
Fragments arranged by size:
514 474 467 111 108
XbaI T'CTAG_A
Cuts at: 0 1437 1584 1674
Size: 1437 147 90
Fragments arranged by size:
1437 147 90
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApoI AseI BamHI BanII
BbsI BciVI BglI BglII BlpI Bme1580I BmrI BmtI
BplI BpmI BpuEI BseYI BsgI BsiHKAI Bsp1286I BspHI
BsrDI BsrGI BstAPI BstXI BstYI BtgI BtsI DraIII
DrdI EaeI EarI EcoICRI Eco57MI EcoRI FalI Hin4I
HincII MfeI MscI MslI MspA1I NcoI NdeI NheI
NspI PciI PshAI PsiI PvuII SacI SapI SfcI
SmlI SpeI SphI StuI StyI TaqII TatI XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BclI BfrBI
BmgBI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspMI BsrBI
BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI EagI EciI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MmeI NaeI NarI NgoMIV NotI NruI NsiI
PacI PasI PflMI PfoI PmeI PmlI PpiI PpuMI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI SspI SwaI TstI Tth111I XcmI
XhoI XmaI XmnI ZraI