Restriction map for
pF1KB5983
(Linear) (Six-base) MAPSORT of: KIBB5983.seq Check: 6005 from: 1 to: 1563
KIBB5983.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 479 551 559 1450 1563
Size: 374 105 72 8 891 113
Fragments arranged by size:
891 374 113 105 72 8
AfeI AGC'GCT
Cuts at: 0 307 1095 1563
Size: 307 788 468
Fragments arranged by size:
788 468 307
AgeI A'CCGG_T
Cuts at: 0 985 1563
Size: 985 578
AlwNI CAG_nnn'CTG
Cuts at: 0 185 1041 1563
Size: 185 856 522
Fragments arranged by size:
856 522 185
ApoI r'AATT_y
Cuts at: 0 22 28 865 1563
Size: 22 6 837 698
Fragments arranged by size:
837 698 22 6
AvaI C'yCGr_G
Cuts at: 0 1550 1563
Size: 1550 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 1177 1563
Size: 1177 386
BbvCI CC'TCA_GC
Cuts at: 0 1103 1563
Size: 1103 460
BglI GCCn_nnn'nGGC
Cuts at: 0 1525 1563
Size: 1525 38
BlpI GC'TnA_GC
Cuts at: 0 1019 1563
Size: 1019 544
Bme1580I G_kGCm'C
Cuts at: 0 1359 1563
Size: 1359 204
BmrI ACTGGGnnnn_n'
Cuts at: 0 923 1563
Size: 923 640
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 939 1302 1563
Size: 939 363 261
Fragments arranged by size:
939 363 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1563
Size: 571 992
Bpu10I CC'TnA_GC
Cuts at: 0 1103 1563
Size: 1103 460
BsaBI GATnn'nnATC
Cuts at: 0 233 1563
Size: 233 1330
BsaWI w'CCGG_w
Cuts at: 0 985 1563
Size: 985 578
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 297 1563
Size: 297 1266
BseYI C'CCAG_C
Cuts at: 0 185 735 1563
Size: 185 550 828
Fragments arranged by size:
828 550 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1563
Size: 1141 422
BsiHKAI G_wGCw'C
Cuts at: 0 289 1563
Size: 289 1274
Bsp1286I G_dGCh'C
Cuts at: 0 289 1359 1563
Size: 289 1070 204
Fragments arranged by size:
1070 289 204
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 1563
Size: 302 1261
BsrDI GCAATG_nn'
Cuts at: 0 1030 1563
Size: 1030 533
BsrFI r'CCGG_y
Cuts at: 0 985 1115 1563
Size: 985 130 448
Fragments arranged by size:
985 448 130
BstEII G'GTnAC_C
Cuts at: 0 1321 1563
Size: 1321 242
BstXI CCAn_nnnn'nTGG
Cuts at: 0 169 1563
Size: 169 1394
BstYI r'GATC_y
Cuts at: 0 143 1563
Size: 143 1420
Bsu36I CC'TnA_GG
Cuts at: 0 280 1563
Size: 280 1283
DraI TTT'AAA
Cuts at: 0 27 1563
Size: 27 1536
EarI CTCTTCn'nnn_
Cuts at: 0 1156 1563
Size: 1156 407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 923 1122 1563
Size: 923 199 441
Fragments arranged by size:
923 441 199
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 479 551 559 571 1450 1563
Size: 374 105 72 8 12 879 113
Fragments arranged by size:
879 374 113 105 72 12 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 1563
Size: 760 803
EcoO109I rG'GnC_Cy
Cuts at: 0 1281 1563
Size: 1281 282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1428 1563
Size: 1428 135
HaeII r_GCGC'y
Cuts at: 0 309 1097 1116 1563
Size: 309 788 19 447
Fragments arranged by size:
788 447 309 19
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 672 704 1563
Size: 364 32 276 32 859
Fragments arranged by size:
859 364 276 32 32
HincII GTy'rAC
Cuts at: 0 753 1563
Size: 753 810
HindIII A'AGCT_T
Cuts at: 0 1294 1563
Size: 1294 269
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1563
Size: 154 1409
MslI CAynn'nnrTG
Cuts at: 0 167 721 1563
Size: 167 554 842
Fragments arranged by size:
842 554 167
MspA1I CmG'CkG
Cuts at: 0 549 1563
Size: 549 1014
NaeI GCC'GGC
Cuts at: 0 1117 1563
Size: 1117 446
NdeI CA'TA_TG
Cuts at: 0 828 1563
Size: 828 735
NgoMIV G'CCGG_C
Cuts at: 0 1115 1563
Size: 1115 448
NspI r_CATG'y
Cuts at: 0 712 1143 1563
Size: 712 431 420
Fragments arranged by size:
712 431 420
PfoI T'CCnGG_A
Cuts at: 0 1288 1563
Size: 1288 275
PpuMI rG'GwC_Cy
Cuts at: 0 1281 1563
Size: 1281 282
PstI C_TGCA'G
Cuts at: 0 313 1563
Size: 313 1250
PvuII CAG'CTG
Cuts at: 0 549 1563
Size: 549 1014
SapI GCTCTTCn'nnn_
Cuts at: 0 1156 1563
Size: 1156 407
SfcI C'TryA_G
Cuts at: 0 269 309 402 518 1563
Size: 269 40 93 116 1045
Fragments arranged by size:
1045 269 116 93 40
SgrAI Cr'CCGG_yG
Cuts at: 0 985 1563
Size: 985 578
SmaI CCC'GGG
Cuts at: 0 1552 1563
Size: 1552 11
SphI G_CATG'C
Cuts at: 0 1143 1563
Size: 1143 420
StuI AGG'CCT
Cuts at: 0 344 684 1563
Size: 344 340 879
Fragments arranged by size:
879 344 340
StyI C'CwwG_G
Cuts at: 0 74 1398 1563
Size: 74 1324 165
Fragments arranged by size:
1324 165 74
SwaI ATTT'AAAT
Cuts at: 0 27 1563
Size: 27 1536
Tth111I GACn'n_nGTC
Cuts at: 0 331 858 1563
Size: 331 527 705
Fragments arranged by size:
705 527 331
XmaI C'CCGG_G
Cuts at: 0 1550 1563
Size: 1550 13
Enzymes that do cut:
AcuI AfeI AgeI AlwNI ApoI AvaI BbsI BbvCI
BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaBI
BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BstEII BstXI BstYI Bsu36I DraI EarI EciI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII
MmeI MslI MspA1I NaeI NdeI NgoMIV NspI PfoI
PpuMI PstI PvuII SapI SfcI SgrAI SmaI SphI
StuI StyI SwaI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BanII BbeI BcgI BciVI
BclI BfrBI BglII BmgBI BmtI BpuEI BsaI BsaAI
BsaHI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraIII DrdI EaeI EagI
EcoICRI EcoRI EcoRV FseI FspI FspAI HpaI KasI
KpnI MfeI MluI MscI NarI NcoI NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SmlI SnaBI SpeI SrfI SspI TaqII
TatI TstI XbaI XcmI XhoI XmnI ZraI