Restriction map for
pF1KB5977
(Linear) (Six-base) MAPSORT of: KIBB5977.seq Check: 3450 from: 1 to: 1557
KIBB5977.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 1557
Size: 1310 247
AflIII A'CryG_T
Cuts at: 0 597 1557
Size: 597 960
AlwNI CAG_nnn'CTG
Cuts at: 0 825 1557
Size: 825 732
ApoI r'AATT_y
Cuts at: 0 529 1557
Size: 529 1028
BamHI G'GATC_C
Cuts at: 0 762 1557
Size: 762 795
BbsI GAAGACnn'nnnn_
Cuts at: 0 130 677 1557
Size: 130 547 880
Fragments arranged by size:
880 547 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1217 1251 1557
Size: 1217 34 306
Fragments arranged by size:
1217 306 34
BfrBI ATG'CAT
Cuts at: 0 910 1557
Size: 910 647
BglI GCCn_nnn'nGGC
Cuts at: 0 478 1557
Size: 478 1079
BglII A'GATC_T
Cuts at: 0 213 1557
Size: 213 1344
BmrI ACTGGGnnnn_n'
Cuts at: 0 1530 1557
Size: 1530 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 35 728 1557
Size: 35 693 829
Fragments arranged by size:
829 693 35
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 107 608 1557
Size: 82 25 501 949
Fragments arranged by size:
949 501 82 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 11 1126 1557
Size: 11 1115 431
Fragments arranged by size:
1115 431 11
BseYI C'CCAG_C
Cuts at: 0 27 1557
Size: 27 1530
BsiEI CG_ry'CG
Cuts at: 0 8 1557
Size: 8 1549
BsmI GAATG_Cn'
Cuts at: 0 474 1528 1557
Size: 474 1054 29
Fragments arranged by size:
1054 474 29
BsrDI GCAATG_nn'
Cuts at: 0 323 1550 1557
Size: 323 1227 7
Fragments arranged by size:
1227 323 7
BstBI TT'CG_AA
Cuts at: 0 1413 1557
Size: 1413 144
BstYI r'GATC_y
Cuts at: 0 57 213 376 441 762 1557
Size: 57 156 163 65 321 795
Fragments arranged by size:
795 321 163 156 65 57
BtsI GCAGTG_nn'
Cuts at: 0 1211 1557
Size: 1211 346
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 33 68 171 206 672 707 1557
Size: 33 35 103 35 466 35 850
Fragments arranged by size:
850 466 103 35 35 35 33
DraI TTT'AAA
Cuts at: 0 1458 1557
Size: 1458 99
EaeI y'GGCC_r
Cuts at: 0 5 1557
Size: 5 1552
EagI C'GGCC_G
Cuts at: 0 5 1557
Size: 5 1552
EarI CTCTTCn'nnn_
Cuts at: 0 380 1557
Size: 380 1177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 107 608 1310 1557
Size: 82 25 501 702 247
Fragments arranged by size:
702 501 247 82 25
EcoO109I rG'GnC_Cy
Cuts at: 0 75 1557
Size: 75 1482
EcoRV GAT'ATC
Cuts at: 0 1400 1557
Size: 1400 157
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 1557
Size: 664 32 861
Fragments arranged by size:
861 664 32
HincII GTy'rAC
Cuts at: 0 876 1557
Size: 876 681
HindIII A'AGCT_T
Cuts at: 0 1142 1557
Size: 1142 415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 1557
Size: 772 785
MslI CAynn'nnrTG
Cuts at: 0 974 1557
Size: 974 583
MspA1I CmG'CkG
Cuts at: 0 27 1024 1509 1557
Size: 27 997 485 48
Fragments arranged by size:
997 485 48 27
NsiI A_TGCA'T
Cuts at: 0 912 1557
Size: 912 645
NspI r_CATG'y
Cuts at: 0 601 1557
Size: 601 956
PciI A'CATG_T
Cuts at: 0 597 1557
Size: 597 960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 1557
Size: 64 32 1461
Fragments arranged by size:
1461 64 32
PpuMI rG'GwC_Cy
Cuts at: 0 75 1557
Size: 75 1482
PshAI GACnn'nnGTC
Cuts at: 0 652 1557
Size: 652 905
PstI C_TGCA'G
Cuts at: 0 147 297 1206 1557
Size: 147 150 909 351
Fragments arranged by size:
909 351 150 147
PvuII CAG'CTG
Cuts at: 0 1024 1509 1557
Size: 1024 485 48
Fragments arranged by size:
1024 485 48
ScaI AGT'ACT
Cuts at: 0 1477 1557
Size: 1477 80
SfcI C'TryA_G
Cuts at: 0 143 293 757 1202 1557
Size: 143 150 464 445 355
Fragments arranged by size:
464 445 355 150 143
SpeI A'CTAG_T
Cuts at: 0 1488 1557
Size: 1488 69
SspI AAT'ATT
Cuts at: 0 592 842 1557
Size: 592 250 715
Fragments arranged by size:
715 592 250
StyI C'CwwG_G
Cuts at: 0 650 986 1557
Size: 650 336 571
Fragments arranged by size:
650 571 336
TatI w'GTAC_w
Cuts at: 0 1475 1557
Size: 1475 82
Tth111I GACn'n_nGTC
Cuts at: 0 871 1557
Size: 871 686
XbaI T'CTAG_A
Cuts at: 0 1081 1557
Size: 1081 476
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 657 1557
Size: 657 900
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BamHI BbsI BcgI BfrBI
BglI BglII BmrI BplI BpmI BseRI BseYI BsiEI
BsmI BsrDI BstBI BstYI BtsI CspCI DraI EaeI
EagI EarI Eco57MI EcoO109I EcoRV Hin4I HincII HindIII
MmeI MslI MspA1I NsiI NspI PciI PpiI PpuMI
PshAI PstI PvuII ScaI SfcI SpeI SspI StyI
TatI Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BanI BanII BbeI BbvCI
BciVI BclI BlpI Bme1580I BmgBI BmtI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI DraIII DrdI EciI EcoICRI EcoNI
EcoRI FalI FseI FspI FspAI HaeII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PflMI PfoI
PmeI PmlI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
StuI SwaI TaqII TstI XhoI XmaI XmnI ZraI