Restriction map for
pF1KB5974
(Linear) (Six-base) MAPSORT of: KIBB5974.seq Check: 3263 from: 1 to: 1680
KIBB5974.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 1180 1680
Size: 1180 500
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 287 303 993 1094 1680
Size: 61 226 16 690 101 586
Fragments arranged by size:
690 586 226 101 61 16
AlwNI CAG_nnn'CTG
Cuts at: 0 1597 1680
Size: 1597 83
ApaLI G'TGCA_C
Cuts at: 0 1628 1680
Size: 1628 52
ApoI r'AATT_y
Cuts at: 0 235 699 1680
Size: 235 464 981
Fragments arranged by size:
981 464 235
BamHI G'GATC_C
Cuts at: 0 324 1680
Size: 324 1356
BbsI GAAGACnn'nnnn_
Cuts at: 0 1435 1680
Size: 1435 245
BclI T'GATC_A
Cuts at: 0 1029 1680
Size: 1029 651
BfrBI ATG'CAT
Cuts at: 0 421 733 1680
Size: 421 312 947
Fragments arranged by size:
947 421 312
BglII A'GATC_T
Cuts at: 0 298 1680
Size: 298 1382
Bme1580I G_kGCm'C
Cuts at: 0 529 1632 1680
Size: 529 1103 48
Fragments arranged by size:
1103 529 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 538 1085 1680
Size: 538 547 595
Fragments arranged by size:
595 547 538
BmtI G_CTAG'C
Cuts at: 0 1202 1680
Size: 1202 478
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 858 1152 1619 1680
Size: 614 244 294 467 61
Fragments arranged by size:
614 467 294 244 61
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1680
Size: 545 1135
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1416 1446 1680
Size: 1416 30 234
Fragments arranged by size:
1416 234 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 626 1382 1680
Size: 43 583 756 298
Fragments arranged by size:
756 583 298 43
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 485 496 1223 1680
Size: 485 11 727 457
Fragments arranged by size:
727 485 457 11
BsiEI CG_ry'CG
Cuts at: 0 131 1680
Size: 131 1549
BsiHKAI G_wGCw'C
Cuts at: 0 1632 1680
Size: 1632 48
Bsp1286I G_dGCh'C
Cuts at: 0 529 1632 1680
Size: 529 1103 48
Fragments arranged by size:
1103 529 48
BsrDI GCAATG_nn'
Cuts at: 0 1212 1680
Size: 1212 468
BsrFI r'CCGG_y
Cuts at: 0 61 1680
Size: 61 1619
BstAPI GCAn_nnn'nTGC
Cuts at: 0 718 988 1680
Size: 718 270 692
Fragments arranged by size:
718 692 270
BstXI CCAn_nnnn'nTGG
Cuts at: 0 405 1680
Size: 405 1275
BstYI r'GATC_y
Cuts at: 0 298 324 1680
Size: 298 26 1356
Fragments arranged by size:
1356 298 26
BstZ17I GTA'TAC
Cuts at: 0 1181 1680
Size: 1181 499
Bsu36I CC'TnA_GG
Cuts at: 0 464 1680
Size: 464 1216
BtgI C'CryG_G
Cuts at: 0 1412 1680
Size: 1412 268
BtsI GCAGTG_nn'
Cuts at: 0 508 1391 1680
Size: 508 883 289
Fragments arranged by size:
883 508 289
DraIII CAC_nnn'GTG
Cuts at: 0 192 1680
Size: 192 1488
EaeI y'GGCC_r
Cuts at: 0 5 1680
Size: 5 1675
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 287 303 614 858 993 1094 1152
Size: 61 226 16 311 244 135 101 58
Cuts at: 1152 1619 1680
Size: 467 61
Fragments arranged by size:
467 311 244 226 135 101 61 61
58 16
EcoO109I rG'GnC_Cy
Cuts at: 0 434 1680
Size: 434 1246
EcoRI G'AATT_C
Cuts at: 0 235 699 1680
Size: 235 464 981
Fragments arranged by size:
981 464 235
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1124 1680
Size: 1124 556
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 283 315 365 397 595 627 1680
Size: 283 32 50 32 198 32 1053
Fragments arranged by size:
1053 283 198 50 32 32 32
HincII GTy'rAC
Cuts at: 0 120 644 1680
Size: 120 524 1036
Fragments arranged by size:
1036 524 120
HpaI GTT'AAC
Cuts at: 0 120 1680
Size: 120 1560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1672 1680
Size: 1672 8
MslI CAynn'nnrTG
Cuts at: 0 254 1405 1680
Size: 254 1151 275
Fragments arranged by size:
1151 275 254
MspA1I CmG'CkG
Cuts at: 0 928 1378 1594 1680
Size: 928 450 216 86
Fragments arranged by size:
928 450 216 86
NheI G'CTAG_C
Cuts at: 0 1198 1680
Size: 1198 482
NsiI A_TGCA'T
Cuts at: 0 423 735 1680
Size: 423 312 945
Fragments arranged by size:
945 423 312
NspI r_CATG'y
Cuts at: 0 1410 1680
Size: 1410 270
PpuMI rG'GwC_Cy
Cuts at: 0 434 1680
Size: 434 1246
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 882 914 1680
Size: 882 32 766
Fragments arranged by size:
882 766 32
PstI C_TGCA'G
Cuts at: 0 972 1161 1680
Size: 972 189 519
Fragments arranged by size:
972 519 189
PvuII CAG'CTG
Cuts at: 0 928 1594 1680
Size: 928 666 86
Fragments arranged by size:
928 666 86
SfcI C'TryA_G
Cuts at: 0 650 809 968 1157 1437 1680
Size: 650 159 159 189 280 243
Fragments arranged by size:
650 280 243 189 159 159
SmlI C'TyrA_G
Cuts at: 0 560 1680
Size: 560 1120
SphI G_CATG'C
Cuts at: 0 1410 1680
Size: 1410 270
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1416 1448 1680
Size: 1416 32 232
Fragments arranged by size:
1416 232 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 811 1680
Size: 811 869
Enzymes that do cut:
AccI AcuI AlwNI ApaLI ApoI BamHI BbsI BclI
BfrBI BglII Bme1580I BmrI BmtI BpmI BpuEI BsaXI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BsrDI BsrFI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII HpaI MmeI
MslI MspA1I NheI NsiI NspI PpuMI PsrI PstI
PvuII SfcI SmlI SphI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbvCI BcgI
BciVI BglI BlpI BmgBI BplI Bpu10I BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII
BtgZI ClaI CspCI DraI DrdI EagI EarI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NotI NruI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI StuI StyI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI ZraI