Restriction map for
pF1KB5949
(Linear) (Six-base) MAPSORT of: KIBB5949.seq Check: 7979 from: 1 to: 1527
KIBB5949.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 1295 1527
Size: 815 480 232
Fragments arranged by size:
815 480 232
AflIII A'CryG_T
Cuts at: 0 1464 1527
Size: 1464 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 473 1527
Size: 473 1054
AlwNI CAG_nnn'CTG
Cuts at: 0 210 1038 1527
Size: 210 828 489
Fragments arranged by size:
828 489 210
ApaLI G'TGCA_C
Cuts at: 0 683 1527
Size: 683 844
ApoI r'AATT_y
Cuts at: 0 325 1127 1527
Size: 325 802 400
Fragments arranged by size:
802 400 325
AseI AT'TA_AT
Cuts at: 0 505 1527
Size: 505 1022
BanII G_rGCy'C
Cuts at: 0 400 1527
Size: 400 1127
BbsI GAAGACnn'nnnn_
Cuts at: 0 1004 1166 1281 1527
Size: 1004 162 115 246
Fragments arranged by size:
1004 246 162 115
BbvCI CC'TCA_GC
Cuts at: 0 33 1527
Size: 33 1494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 502 536 1527
Size: 502 34 991
Fragments arranged by size:
991 502 34
BglI GCCn_nnn'nGGC
Cuts at: 0 43 1527
Size: 43 1484
BglII A'GATC_T
Cuts at: 0 1047 1527
Size: 1047 480
Bme1580I G_kGCm'C
Cuts at: 0 687 1527
Size: 687 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 207 332 1372 1527
Size: 89 118 125 1040 155
Fragments arranged by size:
1040 155 125 118 89
Bpu10I CC'TnA_GC
Cuts at: 0 33 702 1527
Size: 33 669 825
Fragments arranged by size:
825 669 33
BsaAI yAC'GTr
Cuts at: 0 1420 1527
Size: 1420 107
BseYI C'CCAG_C
Cuts at: 0 1056 1527
Size: 1056 471
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 1130 1527
Size: 12 1118 397
Fragments arranged by size:
1118 397 12
BsiHKAI G_wGCw'C
Cuts at: 0 687 1527
Size: 687 840
Bsp1286I G_dGCh'C
Cuts at: 0 400 687 1527
Size: 400 287 840
Fragments arranged by size:
840 400 287
BsrDI GCAATG_nn'
Cuts at: 0 885 1285 1527
Size: 885 400 242
Fragments arranged by size:
885 400 242
BsrFI r'CCGG_y
Cuts at: 0 88 1527
Size: 88 1439
BstAPI GCAn_nnn'nTGC
Cuts at: 0 85 1527
Size: 85 1442
BstEII G'GTnAC_C
Cuts at: 0 91 1527
Size: 91 1436
BstYI r'GATC_y
Cuts at: 0 206 733 1047 1456 1527
Size: 206 527 314 409 71
Fragments arranged by size:
527 409 314 206 71
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 234 1527
Size: 234 1293
EaeI y'GGCC_r
Cuts at: 0 5 44 60 1527
Size: 5 39 16 1467
Fragments arranged by size:
1467 39 16 5
EarI CTCTTCn'nnn_
Cuts at: 0 128 533 1527
Size: 128 405 994
Fragments arranged by size:
994 405 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 207 332 815 1295 1372 1527
Size: 89 118 125 483 480 77 155
Fragments arranged by size:
483 480 155 125 118 89 77
EcoRI G'AATT_C
Cuts at: 0 1127 1527
Size: 1127 400
EcoRV GAT'ATC
Cuts at: 0 984 1527
Size: 984 543
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1183 1325 1527
Size: 1183 142 202
Fragments arranged by size:
1183 202 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1032 1064 1507 1527
Size: 1032 32 443 20
Fragments arranged by size:
1032 443 32 20
HincII GTy'rAC
Cuts at: 0 333 630 818 1527
Size: 333 297 188 709
Fragments arranged by size:
709 333 297 188
HindIII A'AGCT_T
Cuts at: 0 589 1527
Size: 589 938
HpaI GTT'AAC
Cuts at: 0 333 630 1527
Size: 333 297 897
Fragments arranged by size:
897 333 297
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 1527
Size: 123 1404
MspA1I CmG'CkG
Cuts at: 0 109 1060 1527
Size: 109 951 467
Fragments arranged by size:
951 467 109
NspI r_CATG'y
Cuts at: 0 1468 1475 1527
Size: 1468 7 52
Fragments arranged by size:
1468 52 7
PciI A'CATG_T
Cuts at: 0 1464 1527
Size: 1464 63
PfoI T'CCnGG_A
Cuts at: 0 1388 1527
Size: 1388 139
PstI C_TGCA'G
Cuts at: 0 525 1527
Size: 525 1002
PvuII CAG'CTG
Cuts at: 0 109 1060 1527
Size: 109 951 467
Fragments arranged by size:
951 467 109
SapI GCTCTTCn'nnn_
Cuts at: 0 533 1527
Size: 533 994
SfcI C'TryA_G
Cuts at: 0 521 1030 1115 1527
Size: 521 509 85 412
Fragments arranged by size:
521 509 412 85
SspI AAT'ATT
Cuts at: 0 177 576 1527
Size: 177 399 951
Fragments arranged by size:
951 399 177
StyI C'CwwG_G
Cuts at: 0 821 1527
Size: 821 706
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 110 1527
Size: 110 1417
TatI w'GTAC_w
Cuts at: 0 1383 1527
Size: 1383 144
XmnI GAAnn'nnTTC
Cuts at: 0 177 1527
Size: 177 1350
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaLI ApoI AseI BanII
BbsI BbvCI BcgI BglI BglII Bme1580I BpmI Bpu10I
BsaAI BseYI BsgI BsiHKAI Bsp1286I BsrDI BsrFI BstAPI
BstEII BstYI BtgZI EaeI EarI Eco57MI EcoRI EcoRV
FalI Hin4I HincII HindIII HpaI MmeI MspA1I NspI
PciI PfoI PstI PvuII SapI SfcI SspI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AsiSI AvaI AvrII
BaeI BamHI BanI BbeI BciVI BclI BfrBI BlpI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstXI
BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I FseI FspI
FspAI HaeII KasI KpnI MfeI MluI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI