Restriction map for
pF1KB5941
(Linear) (Six-base) MAPSORT of: KIBB5941.seq Check: 3525 from: 1 to: 1524
KIBB5941.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 990 1524
Size: 990 534
AatII G_ACGT'C
Cuts at: 0 526 551 1524
Size: 526 25 973
Fragments arranged by size:
973 526 25
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 853 1524
Size: 853 671
AfeI AGC'GCT
Cuts at: 0 207 1009 1524
Size: 207 802 515
Fragments arranged by size:
802 515 207
AflIII A'CryG_T
Cuts at: 0 338 1524
Size: 338 1186
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1230 1524
Size: 1230 294
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 85 117 1524
Size: 85 32 1407
Fragments arranged by size:
1407 85 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1162 1489 1524
Size: 1162 327 35
Fragments arranged by size:
1162 327 35
ApaI G_GGCC'C
Cuts at: 0 140 220 1060 1524
Size: 140 80 840 464
Fragments arranged by size:
840 464 140 80
AvaI C'yCGr_G
Cuts at: 0 10 131 219 247 858 1524
Size: 10 121 88 28 611 666
Fragments arranged by size:
666 611 121 88 28 10
BanI G'GyrC_C
Cuts at: 0 72 1524
Size: 72 1452
BanII G_rGCy'C
Cuts at: 0 140 220 831 865 955 1060 1428 1524
Size: 140 80 611 34 90 105 368 96
Fragments arranged by size:
611 368 140 105 96 90 80 34
BbeI G_GCGC'C
Cuts at: 0 76 1524
Size: 76 1448
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 333 367 1524
Size: 333 34 1157
Fragments arranged by size:
1157 333 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1089 1524
Size: 1089 435
BglI GCCn_nnn'nGGC
Cuts at: 0 70 124 1524
Size: 70 54 1400
Fragments arranged by size:
1400 70 54
BglII A'GATC_T
Cuts at: 0 842 1524
Size: 842 682
BlpI GC'TnA_GC
Cuts at: 0 619 674 1490 1524
Size: 619 55 816 34
Fragments arranged by size:
816 619 55 34
Bme1580I G_kGCm'C
Cuts at: 0 140 220 356 1060 1327 1524
Size: 140 80 136 704 267 197
Fragments arranged by size:
704 267 197 140 136 80
BmgBI CAC'GTC
Cuts at: 0 341 1524
Size: 341 1183
BmrI ACTGGGnnnn_n'
Cuts at: 0 1136 1524
Size: 1136 388
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1272 1524
Size: 1272 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 717 1087 1524
Size: 616 101 370 437
Fragments arranged by size:
616 437 370 101
Bpu10I CC'TnA_GC
Cuts at: 0 1328 1407 1524
Size: 1328 79 117
Fragments arranged by size:
1328 117 79
BsaAI yAC'GTr
Cuts at: 0 483 1524
Size: 483 1041
BsaBI GATnn'nnATC
Cuts at: 0 1100 1524
Size: 1100 424
BsaHI Gr'CG_yC
Cuts at: 0 16 73 437 523 548 730 1524
Size: 16 57 364 86 25 182 794
Fragments arranged by size:
794 364 182 86 57 25 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 703 733 1279 1309 1524
Size: 220 30 453 30 546 30 215
Fragments arranged by size:
546 453 220 215 30 30 30
BseYI C'CCAG_C
Cuts at: 0 58 208 1013 1240 1291 1298 1482 1504
Size: 58 150 805 227 51 7 184 22
Cuts at: 1504 1524
Size: 20
Fragments arranged by size:
805 227 184 150 58 51 22 20
7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 942 1524
Size: 262 680 582
Fragments arranged by size:
680 582 262
BsiEI CG_ry'CG
Cuts at: 0 557 1524
Size: 557 967
BsiHKAI G_wGCw'C
Cuts at: 0 431 806 1524
Size: 431 375 718
Fragments arranged by size:
718 431 375
BsmBI CGTCTCn'nnnn_
Cuts at: 0 530 555 1524
Size: 530 25 969
Fragments arranged by size:
969 530 25
Bsp1286I G_dGCh'C
Cuts at: 0 140 220 356 431 806 831 865 955
Size: 140 80 136 75 375 25 34 90
Cuts at: 955 1060 1327 1428 1524
Size: 105 267 101 96
Fragments arranged by size:
375 267 140 136 105 101 96 90
80 75 34 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 635 990 1524
Size: 635 355 534
Fragments arranged by size:
635 534 355
BsrBI CCG'CTC
Cuts at: 0 165 476 1524
Size: 165 311 1048
Fragments arranged by size:
1048 311 165
BsrFI r'CCGG_y
Cuts at: 0 556 1524
Size: 556 968
BssHII G'CGCG_C
Cuts at: 0 662 1524
Size: 662 862
BssSI C'ACGA_G
Cuts at: 0 296 775 1524
Size: 296 479 749
Fragments arranged by size:
749 479 296
BstXI CCAn_nnnn'nTGG
Cuts at: 0 934 1512 1524
Size: 934 578 12
Fragments arranged by size:
934 578 12
BstYI r'GATC_y
Cuts at: 0 590 842 1524
Size: 590 252 682
Fragments arranged by size:
682 590 252
BtgI C'CryG_G
Cuts at: 0 259 290 443 1187 1312 1524
Size: 259 31 153 744 125 212
Fragments arranged by size:
744 259 212 153 125 31
BtsI GCAGTG_nn'
Cuts at: 0 1037 1086 1176 1524
Size: 1037 49 90 348
Fragments arranged by size:
1037 348 90 49
DraIII CAC_nnn'GTG
Cuts at: 0 486 611 1524
Size: 486 125 913
Fragments arranged by size:
913 486 125
EaeI y'GGCC_r
Cuts at: 0 477 554 1184 1524
Size: 477 77 630 340
Fragments arranged by size:
630 477 340 77
EagI C'GGCC_G
Cuts at: 0 554 1524
Size: 554 970
EarI CTCTTCn'nnn_
Cuts at: 0 137 892 1524
Size: 137 755 632
Fragments arranged by size:
755 632 137
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 717 853 1087 1524
Size: 616 101 136 234 437
Fragments arranged by size:
616 437 234 136 101
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 1524
Size: 280 1244
EcoO109I rG'GnC_Cy
Cuts at: 0 136 975 1056 1057 1149 1159 1524
Size: 136 839 81 1 92 10 365
Fragments arranged by size:
839 365 136 92 81 10 1
EcoRV GAT'ATC
Cuts at: 0 397 1524
Size: 397 1127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 924 1524
Size: 924 600
HaeII r_GCGC'y
Cuts at: 0 76 209 389 704 1011 1524
Size: 76 133 180 315 307 513
Fragments arranged by size:
513 315 307 180 133 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 1183 1215 1279 1311 1390 1422
Size: 703 32 448 32 64 32 79 32
Cuts at: 1422 1524
Size: 102
Fragments arranged by size:
703 448 102 79 64 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 72 1524
Size: 72 1452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 689 1481 1524
Size: 149 540 792 43
Fragments arranged by size:
792 540 149 43
MscI TGG'CCA
Cuts at: 0 1186 1524
Size: 1186 338
MslI CAynn'nnrTG
Cuts at: 0 416 775 787 1524
Size: 416 359 12 737
Fragments arranged by size:
737 416 359 12
MspA1I CmG'CkG
Cuts at: 0 915 1489 1524
Size: 915 574 35
Fragments arranged by size:
915 574 35
NaeI GCC'GGC
Cuts at: 0 558 1524
Size: 558 966
NarI GG'CG_CC
Cuts at: 0 73 1524
Size: 73 1451
NcoI C'CATG_G
Cuts at: 0 259 1187 1312 1524
Size: 259 928 125 212
Fragments arranged by size:
928 259 212 125
NgoMIV G'CCGG_C
Cuts at: 0 556 1524
Size: 556 968
NspI r_CATG'y
Cuts at: 0 1090 1524
Size: 1090 434
PasI CC'CwG_GG
Cuts at: 0 1156 1419 1524
Size: 1156 263 105
Fragments arranged by size:
1156 263 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 1162 1524
Size: 1162 362
PfoI T'CCnGG_A
Cuts at: 0 89 593 1283 1524
Size: 89 504 690 241
Fragments arranged by size:
690 504 241 89
PpuMI rG'GwC_Cy
Cuts at: 0 975 1149 1524
Size: 975 174 375
Fragments arranged by size:
975 375 174
PspOMI G'GGCC_C
Cuts at: 0 136 216 1056 1524
Size: 136 80 840 468
Fragments arranged by size:
840 468 136 80
PspXI vC'TCGA_Gb
Cuts at: 0 247 1524
Size: 247 1277
PvuII CAG'CTG
Cuts at: 0 915 1524
Size: 915 609
SapI GCTCTTCn'nnn_
Cuts at: 0 137 1524
Size: 137 1387
SfcI C'TryA_G
Cuts at: 0 228 329 1401 1524
Size: 228 101 1072 123
Fragments arranged by size:
1072 228 123 101
SfoI GGC'GCC
Cuts at: 0 74 1524
Size: 74 1450
SmaI CCC'GGG
Cuts at: 0 133 221 1524
Size: 133 88 1303
Fragments arranged by size:
1303 133 88
SmlI C'TyrA_G
Cuts at: 0 247 1524
Size: 247 1277
SphI G_CATG'C
Cuts at: 0 1090 1524
Size: 1090 434
SrfI GCCC'GGGC
Cuts at: 0 221 1524
Size: 221 1303
StuI AGG'CCT
Cuts at: 0 884 1524
Size: 884 640
StyI C'CwwG_G
Cuts at: 0 259 1187 1312 1428 1524
Size: 259 928 125 116 96
Fragments arranged by size:
928 259 125 116 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 531 910 1524
Size: 531 379 614
Fragments arranged by size:
614 531 379
TatI w'GTAC_w
Cuts at: 0 1274 1524
Size: 1274 250
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 166 198 1524
Size: 166 32 1326
Fragments arranged by size:
1326 166 32
XhoI C'TCGA_G
Cuts at: 0 247 1524
Size: 247 1277
XmaI C'CCGG_G
Cuts at: 0 131 219 1524
Size: 131 88 1305
Fragments arranged by size:
1305 131 88
XmnI GAAnn'nnTTC
Cuts at: 0 146 1524
Size: 146 1378
ZraI GAC'GTC
Cuts at: 0 524 549 1524
Size: 524 25 975
Fragments arranged by size:
975 524 25
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AlfI AloI AlwNI
ApaI AvaI BanI BanII BbeI BcgI BciVI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI BstXI
BstYI BtgI BtsI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I EcoRV FalI HaeII Hin4I KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PflMI PfoI PpuMI PspOMI PspXI PvuII SapI
SfcI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AleI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI
BbvCI BclI BfrBI BmtI BpuEI BsaI BsaWI BseRI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EciI EcoICRI EcoRI FseI FspI FspAI HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SspI SwaI Tth111I XbaI XcmI