Restriction map for
pF1KB5937
(Linear) (Six-base) MAPSORT of: KIBB5937.seq Check: 445 from: 1 to: 1524
KIBB5937.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 294 518 529 1297 1524
Size: 115 179 224 11 768 227
Fragments arranged by size:
768 227 224 179 115 11
AflIII A'CryG_T
Cuts at: 0 1352 1524
Size: 1352 172
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 709 741 1524
Size: 14 32 663 32 783
Fragments arranged by size:
783 663 32 32 14
AlwNI CAG_nnn'CTG
Cuts at: 0 138 1524
Size: 138 1386
ApaI G_GGCC'C
Cuts at: 0 368 1524
Size: 368 1156
AvaI C'yCGr_G
Cuts at: 0 212 368 1302 1345 1524
Size: 212 156 934 43 179
Fragments arranged by size:
934 212 179 156 43
BanI G'GyrC_C
Cuts at: 0 115 1524
Size: 115 1409
BanII G_rGCy'C
Cuts at: 0 368 580 763 1244 1524
Size: 368 212 183 481 280
Fragments arranged by size:
481 368 280 212 183
BbeI G_GCGC'C
Cuts at: 0 119 1524
Size: 119 1405
BbsI GAAGACnn'nnnn_
Cuts at: 0 517 1302 1524
Size: 517 785 222
Fragments arranged by size:
785 517 222
BbvCI CC'TCA_GC
Cuts at: 0 475 1524
Size: 475 1049
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 190 224 1524
Size: 190 34 1300
Fragments arranged by size:
1300 190 34
BclI T'GATC_A
Cuts at: 0 1397 1524
Size: 1397 127
BglI GCCn_nnn'nGGC
Cuts at: 0 76 1338 1524
Size: 76 1262 186
Fragments arranged by size:
1262 186 76
BglII A'GATC_T
Cuts at: 0 707 1524
Size: 707 817
BlpI GC'TnA_GC
Cuts at: 0 83 574 1524
Size: 83 491 950
Fragments arranged by size:
950 491 83
Bme1580I G_kGCm'C
Cuts at: 0 368 1524
Size: 368 1156
BmrI ACTGGGnnnn_n'
Cuts at: 0 189 287 528 690 1524
Size: 189 98 241 162 834
Fragments arranged by size:
834 241 189 162 98
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 606 1520 1524
Size: 606 914 4
Fragments arranged by size:
914 606 4
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 403 576 736 1402 1524
Size: 61 342 173 160 666 122
Fragments arranged by size:
666 342 173 160 122 61
Bpu10I CC'TnA_GC
Cuts at: 0 475 1524
Size: 475 1049
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 667 1524
Size: 667 857
BsaI GGTCTCn'nnnn_
Cuts at: 0 1072 1147 1524
Size: 1072 75 377
Fragments arranged by size:
1072 377 75
BsaHI Gr'CG_yC
Cuts at: 0 46 116 779 1088 1524
Size: 46 70 663 309 436
Fragments arranged by size:
663 436 309 70 46
BsaWI w'CCGG_w
Cuts at: 0 1038 1070 1524
Size: 1038 32 454
Fragments arranged by size:
1038 454 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 14 44 709 739 1524
Size: 14 30 665 30 785
Fragments arranged by size:
785 665 30 30 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 480 1524
Size: 217 263 1044
Fragments arranged by size:
1044 263 217
BseYI C'CCAG_C
Cuts at: 0 87 138 450 794 804 1191 1243 1524
Size: 87 51 312 344 10 387 52 281
Fragments arranged by size:
387 344 312 281 87 52 51 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 1524
Size: 427 1097
BsiEI CG_ry'CG
Cuts at: 0 1062 1524
Size: 1062 462
BsiHKAI G_wGCw'C
Cuts at: 0 101 763 1524
Size: 101 662 761
Fragments arranged by size:
761 662 101
BsmI GAATG_Cn'
Cuts at: 0 248 1524
Size: 248 1276
Bsp1286I G_dGCh'C
Cuts at: 0 101 368 580 763 1244 1524
Size: 101 267 212 183 481 280
Fragments arranged by size:
481 280 267 212 183 101
BspEI T'CCGG_A
Cuts at: 0 1070 1524
Size: 1070 454
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1457 1524
Size: 1457 67
BsrBI CCG'CTC
Cuts at: 0 692 1524
Size: 692 832
BsrDI GCAATG_nn'
Cuts at: 0 1361 1524
Size: 1361 163
BsrFI r'CCGG_y
Cuts at: 0 611 1524
Size: 611 913
BstBI TT'CG_AA
Cuts at: 0 243 1524
Size: 243 1281
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1098 1524
Size: 1098 426
BstYI r'GATC_y
Cuts at: 0 707 840 1524
Size: 707 133 684
Fragments arranged by size:
707 684 133
BtgI C'CryG_G
Cuts at: 0 642 1334 1524
Size: 642 692 190
Fragments arranged by size:
692 642 190
BtsI GCAGTG_nn'
Cuts at: 0 540 1524
Size: 540 984
DraIII CAC_nnn'GTG
Cuts at: 0 1443 1524
Size: 1443 81
EaeI y'GGCC_r
Cuts at: 0 663 1524
Size: 663 861
EarI CTCTTCn'nnn_
Cuts at: 0 118 475 790 1524
Size: 118 357 315 734
Fragments arranged by size:
734 357 315 118
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 34 1147 1524
Size: 19 15 1113 377
Fragments arranged by size:
1113 377 19 15
EcoICRI GAG'CTC
Cuts at: 0 761 1524
Size: 761 763
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 115 294 403 518 529 576 736
Size: 61 54 179 109 115 11 47 160
Cuts at: 736 1297 1402 1524
Size: 561 105 122
Fragments arranged by size:
561 179 160 122 115 109 105 61
54 47 11
EcoO109I rG'GnC_Cy
Cuts at: 0 261 365 852 1371 1499 1524
Size: 261 104 487 519 128 25
Fragments arranged by size:
519 487 261 128 104 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1047 1524
Size: 1047 477
HaeII r_GCGC'y
Cuts at: 0 71 119 139 998 1389 1524
Size: 71 48 20 859 391 135
Fragments arranged by size:
859 391 135 71 48 20
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 885 917 1339 1371 1524
Size: 885 32 422 32 153
Fragments arranged by size:
885 422 153 32 32
KasI G'GCGC_C
Cuts at: 0 115 1524
Size: 115 1409
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 350 726 1275 1524
Size: 350 376 549 249
Fragments arranged by size:
549 376 350 249
MslI CAynn'nnrTG
Cuts at: 0 733 1252 1524
Size: 733 519 272
Fragments arranged by size:
733 519 272
MspA1I CmG'CkG
Cuts at: 0 411 423 662 678 847 882 1524
Size: 411 12 239 16 169 35 642
Fragments arranged by size:
642 411 239 169 35 16 12
NarI GG'CG_CC
Cuts at: 0 116 1524
Size: 116 1408
NcoI C'CATG_G
Cuts at: 0 1334 1524
Size: 1334 190
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 179 332 1161 1524
Size: 108 71 153 829 363
Fragments arranged by size:
829 363 153 108 71
PfoI T'CCnGG_A
Cuts at: 0 713 1421 1524
Size: 713 708 103
Fragments arranged by size:
713 708 103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 14 46 709 741 1524
Size: 14 32 663 32 783
Fragments arranged by size:
783 663 32 32 14
PspOMI G'GGCC_C
Cuts at: 0 364 1524
Size: 364 1160
PstI C_TGCA'G
Cuts at: 0 860 1454 1524
Size: 860 594 70
Fragments arranged by size:
860 594 70
PvuII CAG'CTG
Cuts at: 0 423 1524
Size: 423 1101
SacI G_AGCT'C
Cuts at: 0 763 1524
Size: 763 761
SapI GCTCTTCn'nnn_
Cuts at: 0 118 1524
Size: 118 1406
ScaI AGT'ACT
Cuts at: 0 1048 1524
Size: 1048 476
SfcI C'TryA_G
Cuts at: 0 856 1450 1524
Size: 856 594 74
Fragments arranged by size:
856 594 74
SfoI GGC'GCC
Cuts at: 0 117 1524
Size: 117 1407
SmaI CCC'GGG
Cuts at: 0 214 370 1524
Size: 214 156 1154
Fragments arranged by size:
1154 214 156
SmlI C'TyrA_G
Cuts at: 0 682 1524
Size: 682 842
StuI AGG'CCT
Cuts at: 0 1274 1524
Size: 1274 250
StyI C'CwwG_G
Cuts at: 0 1148 1269 1334 1524
Size: 1148 121 65 190
Fragments arranged by size:
1148 190 121 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 155 510 818 1275 1347 1524
Size: 155 355 308 457 72 177
Fragments arranged by size:
457 355 308 177 155 72
TatI w'GTAC_w
Cuts at: 0 1046 1524
Size: 1046 478
Tth111I GACn'n_nGTC
Cuts at: 0 874 1524
Size: 874 650
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 300 1524
Size: 300 1224
XmaI C'CCGG_G
Cuts at: 0 212 368 1524
Size: 212 156 1156
Fragments arranged by size:
1156 212 156
XmnI GAAnn'nnTTC
Cuts at: 0 1030 1524
Size: 1030 494
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstBI BstXI
BstYI BtgI BtsI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I KasI MmeI MslI
MspA1I NarI NcoI PflMI PfoI PpiI PspOMI PstI
PvuII SacI SapI ScaI SfcI SfoI SmaI SmlI
StuI StyI TaqII TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BciVI BfrBI BmgBI BmtI BsaAI
BsaBI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstAPI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EagI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TstI XbaI XhoI ZraI