Restriction map for
pF1KB5923
(Linear) (Six-base) MAPSORT of: KIBB5923.seq Check: 2621 from: 1 to: 1515
KIBB5923.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AccI GT'mk_AC
Cuts at: 0 1372 1515
Size: 1372 143
Acc65I G'GTAC_C
Cuts at: 0 1411 1515
Size: 1411 104
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 901 1515
Size: 547 354 614
Fragments arranged by size:
614 547 354
AflII C'TTAA_G
Cuts at: 0 1162 1515
Size: 1162 353
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1006 1515
Size: 1006 509
ApaI G_GGCC'C
Cuts at: 0 1407 1515
Size: 1407 108
ApaLI G'TGCA_C
Cuts at: 0 782 1515
Size: 782 733
ApoI r'AATT_y
Cuts at: 0 958 1515
Size: 958 557
AvaI C'yCGr_G
Cuts at: 0 188 229 743 881 1112 1139 1406 1418
Size: 188 41 514 138 231 27 267 12
Cuts at: 1418 1515
Size: 97
Fragments arranged by size:
514 267 231 188 138 97 41 27
12
AvrII C'CTAG_G
Cuts at: 0 418 1515
Size: 418 1097
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 398 431 1210 1243 1515
Size: 398 33 779 33 272
Fragments arranged by size:
779 398 272 33 33
BanI G'GyrC_C
Cuts at: 0 99 1411 1515
Size: 99 1312 104
Fragments arranged by size:
1312 104 99
BanII G_rGCy'C
Cuts at: 0 43 136 229 1407 1515
Size: 43 93 93 1178 108
Fragments arranged by size:
1178 108 93 93 43
BbeI G_GCGC'C
Cuts at: 0 103 1515
Size: 103 1412
BbsI GAAGACnn'nnnn_
Cuts at: 0 653 907 1470 1515
Size: 653 254 563 45
Fragments arranged by size:
653 563 254 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 198 232 1515
Size: 198 34 1283
Fragments arranged by size:
1283 198 34
BglI GCCn_nnn'nGGC
Cuts at: 0 97 1515
Size: 97 1418
BlpI GC'TnA_GC
Cuts at: 0 223 1515
Size: 223 1292
Bme1580I G_kGCm'C
Cuts at: 0 786 1407 1515
Size: 786 621 108
Fragments arranged by size:
786 621 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 468 1515
Size: 468 1047
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 186 327 1087 1515
Size: 101 85 141 760 428
Fragments arranged by size:
760 428 141 101 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 991 1515
Size: 991 524
BsaI GGTCTCn'nnnn_
Cuts at: 0 1145 1415 1515
Size: 1145 270 100
Fragments arranged by size:
1145 270 100
BsaAI yAC'GTr
Cuts at: 0 819 1515
Size: 819 696
BsaHI Gr'CG_yC
Cuts at: 0 100 166 1434 1515
Size: 100 66 1268 81
Fragments arranged by size:
1268 100 81 66
BsaWI w'CCGG_w
Cuts at: 0 854 1515
Size: 854 661
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 414 1515
Size: 414 1101
BseYI C'CCAG_C
Cuts at: 0 148 1002 1043 1452 1515
Size: 148 854 41 409 63
Fragments arranged by size:
854 409 148 63 41
BsiEI CG_ry'CG
Cuts at: 0 23 1515
Size: 23 1492
BsiHKAI G_wGCw'C
Cuts at: 0 43 313 786 1250 1515
Size: 43 270 473 464 265
Fragments arranged by size:
473 464 270 265 43
BsmI GAATG_Cn'
Cuts at: 0 717 1515
Size: 717 798
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1441 1515
Size: 1441 74
Bsp1286I G_dGCh'C
Cuts at: 0 43 136 229 313 786 1250 1407 1515
Size: 43 93 93 84 473 464 157 108
Fragments arranged by size:
473 464 157 108 93 93 84 43
BspEI T'CCGG_A
Cuts at: 0 854 1515
Size: 854 661
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 89 809 1358 1515
Size: 89 720 549 157
Fragments arranged by size:
720 549 157 89
BsrBI CCG'CTC
Cuts at: 0 115 155 750 1515
Size: 115 40 595 765
Fragments arranged by size:
765 595 115 40
BsrFI r'CCGG_y
Cuts at: 0 1039 1515
Size: 1039 476
BssSI C'ACGA_G
Cuts at: 0 682 1515
Size: 682 833
BstEII G'GTnAC_C
Cuts at: 0 1134 1515
Size: 1134 381
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1002 1515
Size: 1002 513
BstYI r'GATC_y
Cuts at: 0 1071 1515
Size: 1071 444
BstZ17I GTA'TAC
Cuts at: 0 1373 1515
Size: 1373 142
Bsu36I CC'TnA_GG
Cuts at: 0 1214 1515
Size: 1214 301
BtgI C'CryG_G
Cuts at: 0 23 1425 1515
Size: 23 1402 90
Fragments arranged by size:
1402 90 23
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 505 1515
Size: 505 1010
BtsI GCAGTG_nn'
Cuts at: 0 960 1248 1515
Size: 960 288 267
Fragments arranged by size:
960 288 267
DraIII CAC_nnn'GTG
Cuts at: 0 36 765 1515
Size: 36 729 750
Fragments arranged by size:
750 729 36
DrdI GACnn_nn'nnGTC
Cuts at: 0 1474 1515
Size: 1474 41
EaeI y'GGCC_r
Cuts at: 0 20 756 821 1121 1515
Size: 20 736 65 300 394
Fragments arranged by size:
736 394 300 65 20
EagI C'GGCC_G
Cuts at: 0 20 1515
Size: 20 1495
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 44 503 1515
Size: 44 459 1012
Fragments arranged by size:
1012 459 44
EcoICRI GAG'CTC
Cuts at: 0 41 1515
Size: 41 1474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 186 327 547 901 1087 1515
Size: 101 85 141 220 354 186 428
Fragments arranged by size:
428 354 220 186 141 101 85
EcoNI CCTnn'n_nnAGG
Cuts at: 0 431 1515
Size: 431 1084
EcoO109I rG'GnC_Cy
Cuts at: 0 462 546 567 608 1403 1515
Size: 462 84 21 41 795 112
Fragments arranged by size:
795 462 112 84 41 21
EcoRI G'AATT_C
Cuts at: 0 958 1515
Size: 958 557
HaeII r_GCGC'y
Cuts at: 0 53 103 127 909 1515
Size: 53 50 24 782 606
Fragments arranged by size:
782 606 53 50 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1456 1488 1515
Size: 1456 32 27
Fragments arranged by size:
1456 32 27
KasI G'GCGC_C
Cuts at: 0 99 1515
Size: 99 1416
KpnI G_GTAC'C
Cuts at: 0 1415 1515
Size: 1415 100
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 1515
Size: 376 1139
MscI TGG'CCA
Cuts at: 0 758 823 1123 1515
Size: 758 65 300 392
Fragments arranged by size:
758 392 300 65
MslI CAynn'nnrTG
Cuts at: 0 874 1132 1515
Size: 874 258 383
Fragments arranged by size:
874 383 258
MspA1I CmG'CkG
Cuts at: 0 222 921 1515
Size: 222 699 594
Fragments arranged by size:
699 594 222
NarI GG'CG_CC
Cuts at: 0 100 1515
Size: 100 1415
NspI r_CATG'y
Cuts at: 0 481 1515
Size: 481 1034
PasI CC'CwG_GG
Cuts at: 0 1491 1515
Size: 1491 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 726 765 1392 1515
Size: 726 39 627 123
Fragments arranged by size:
726 627 123 39
PpuMI rG'GwC_Cy
Cuts at: 0 546 567 608 1515
Size: 546 21 41 907
Fragments arranged by size:
907 546 41 21
PspOMI G'GGCC_C
Cuts at: 0 1403 1515
Size: 1403 112
PspXI vC'TCGA_Gb
Cuts at: 0 188 1515
Size: 188 1327
PstI C_TGCA'G
Cuts at: 0 221 1174 1515
Size: 221 953 341
Fragments arranged by size:
953 341 221
PvuII CAG'CTG
Cuts at: 0 222 921 1515
Size: 222 699 594
Fragments arranged by size:
699 594 222
SacI G_AGCT'C
Cuts at: 0 43 1515
Size: 43 1472
SanDI GG'GwC_CC
Cuts at: 0 608 1515
Size: 608 907
SexAI A'CCwGG_T
Cuts at: 0 32 548 1515
Size: 32 516 967
Fragments arranged by size:
967 516 32
SfcI C'TryA_G
Cuts at: 0 217 1170 1515
Size: 217 953 345
Fragments arranged by size:
953 345 217
SfoI GGC'GCC
Cuts at: 0 101 1515
Size: 101 1414
SmaI CCC'GGG
Cuts at: 0 231 745 883 1408 1515
Size: 231 514 138 525 107
Fragments arranged by size:
525 514 231 138 107
SmlI C'TyrA_G
Cuts at: 0 188 970 1162 1515
Size: 188 782 192 353
Fragments arranged by size:
782 353 192 188
SphI G_CATG'C
Cuts at: 0 481 1515
Size: 481 1034
StyI C'CwwG_G
Cuts at: 0 418 995 1515
Size: 418 577 520
Fragments arranged by size:
577 520 418
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 222 979 1515
Size: 222 757 536
Fragments arranged by size:
757 536 222
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1449 1515
Size: 1449 66
XhoI C'TCGA_G
Cuts at: 0 188 1515
Size: 188 1327
XmaI C'CCGG_G
Cuts at: 0 229 743 881 1406 1515
Size: 229 514 138 525 109
Fragments arranged by size:
525 514 229 138 109
XmnI GAAnn'nnTTC
Cuts at: 0 715 869 1515
Size: 715 154 646
Fragments arranged by size:
715 646 154
Enzymes that do cut:
AccI Acc65I AcuI AflII AlfI ApaI ApaLI ApoI
AvaI AvrII BaeI BanI BanII BbeI BbsI BcgI
BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI DraIII DrdI
EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I
NarI NspI PasI PflMI PpuMI PspOMI PspXI PstI
PvuII SacI SanDI SexAI SfcI SfoI SmaI SmlI
SphI StyI TaqII XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
AloI AlwNI AscI AseI AsiSI BamHI BbvCI BciVI
BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaBI
BsaXI BsgI BsiWI BspHI BsrDI BsrGI BssHII BstAPI
BstBI ClaI CspCI DraI EarI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI ZraI