Restriction map for
pF1KB5906
(Linear) (Six-base) MAPSORT of: KIBB5906.seq Check: 2453 from: 1 to: 1578
KIBB5906.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1018 1019 1578
Size: 125 893 1 559
Fragments arranged by size:
893 559 125 1
ApoI r'AATT_y
Cuts at: 0 790 1072 1578
Size: 790 282 506
Fragments arranged by size:
790 506 282
AseI AT'TA_AT
Cuts at: 0 965 1578
Size: 965 613
AvaI C'yCGr_G
Cuts at: 0 61 327 803 1578
Size: 61 266 476 775
Fragments arranged by size:
775 476 266 61
BanII G_rGCy'C
Cuts at: 0 259 1578
Size: 259 1319
BglI GCCn_nnn'nGGC
Cuts at: 0 746 1184 1578
Size: 746 438 394
Fragments arranged by size:
746 438 394
BmgBI CAC'GTC
Cuts at: 0 820 1578
Size: 820 758
BmrI ACTGGGnnnn_n'
Cuts at: 0 1226 1578
Size: 1226 352
BsaI GGTCTCn'nnnn_
Cuts at: 0 1020 1101 1578
Size: 1020 81 477
Fragments arranged by size:
1020 477 81
BsaWI w'CCGG_w
Cuts at: 0 1078 1578
Size: 1078 500
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1558 1578
Size: 1558 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1178 1235 1442 1578
Size: 1178 57 207 136
Fragments arranged by size:
1178 207 136 57
BseYI C'CCAG_C
Cuts at: 0 56 77 316 1578
Size: 56 21 239 1262
Fragments arranged by size:
1262 239 56 21
BsiHKAI G_wGCw'C
Cuts at: 0 259 1550 1578
Size: 259 1291 28
Fragments arranged by size:
1291 259 28
BsmI GAATG_Cn'
Cuts at: 0 1333 1578
Size: 1333 245
Bsp1286I G_dGCh'C
Cuts at: 0 259 1550 1578
Size: 259 1291 28
Fragments arranged by size:
1291 259 28
BspEI T'CCGG_A
Cuts at: 0 1078 1578
Size: 1078 500
BspHI T'CATG_A
Cuts at: 0 822 1578
Size: 822 756
BsrDI GCAATG_nn'
Cuts at: 0 1146 1578
Size: 1146 432
BsrGI T'GTAC_A
Cuts at: 0 971 1578
Size: 971 607
BstXI CCAn_nnnn'nTGG
Cuts at: 0 40 223 601 1578
Size: 40 183 378 977
Fragments arranged by size:
977 378 183 40
Bsu36I CC'TnA_GG
Cuts at: 0 455 1578
Size: 455 1123
BtgI C'CryG_G
Cuts at: 0 131 636 645 741 774 1172 1179 1458
Size: 131 505 9 96 33 398 7 279
Cuts at: 1458 1470 1578
Size: 12 108
Fragments arranged by size:
505 398 279 131 108 96 33 12
9 7
BtsI GCAGTG_nn'
Cuts at: 0 498 573 666 711 1209 1578
Size: 498 75 93 45 498 369
Fragments arranged by size:
498 498 369 93 75 45
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1128 1163 1578
Size: 1128 35 415
Fragments arranged by size:
1128 415 35
EaeI y'GGCC_r
Cuts at: 0 150 174 300 531 738 1215 1578
Size: 150 24 126 231 207 477 363
Fragments arranged by size:
477 363 231 207 150 126 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 779 1578
Size: 779 799
EcoICRI GAG'CTC
Cuts at: 0 257 1578
Size: 257 1321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 1018 1019 1578
Size: 125 893 1 559
Fragments arranged by size:
893 559 125 1
EcoO109I rG'GnC_Cy
Cuts at: 0 44 1168 1344 1578
Size: 44 1124 176 234
Fragments arranged by size:
1124 234 176 44
EcoRI G'AATT_C
Cuts at: 0 1072 1578
Size: 1072 506
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 799 831 1578
Size: 529 32 238 32 747
Fragments arranged by size:
747 529 238 32 32
HincII GTy'rAC
Cuts at: 0 209 1578
Size: 209 1369
MfeI C'AATT_G
Cuts at: 0 890 1578
Size: 890 688
MscI TGG'CCA
Cuts at: 0 152 176 302 533 1578
Size: 152 24 126 231 1045
Fragments arranged by size:
1045 231 152 126 24
MslI CAynn'nnrTG
Cuts at: 0 862 1578
Size: 862 716
MspA1I CmG'CkG
Cuts at: 0 614 647 1460 1578
Size: 614 33 813 118
Fragments arranged by size:
813 614 118 33
NcoI C'CATG_G
Cuts at: 0 1172 1578
Size: 1172 406
PasI CC'CwG_GG
Cuts at: 0 216 1578
Size: 216 1362
PpuMI rG'GwC_Cy
Cuts at: 0 1168 1578
Size: 1168 410
SacI G_AGCT'C
Cuts at: 0 259 1578
Size: 259 1319
SacII CC_GC'GG
Cuts at: 0 648 1461 1578
Size: 648 813 117
Fragments arranged by size:
813 648 117
SexAI A'CCwGG_T
Cuts at: 0 1374 1578
Size: 1374 204
SfcI C'TryA_G
Cuts at: 0 387 1578
Size: 387 1191
SmaI CCC'GGG
Cuts at: 0 63 1578
Size: 63 1515
SrfI GCCC'GGGC
Cuts at: 0 63 1578
Size: 63 1515
StuI AGG'CCT
Cuts at: 0 872 1578
Size: 872 706
StyI C'CwwG_G
Cuts at: 0 810 1172 1578
Size: 810 362 406
Fragments arranged by size:
810 406 362
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 70 865 1578
Size: 70 795 713
Fragments arranged by size:
795 713 70
TatI w'GTAC_w
Cuts at: 0 479 971 1578
Size: 479 492 607
Fragments arranged by size:
607 492 479
XmaI C'CCGG_G
Cuts at: 0 61 1578
Size: 61 1517
Enzymes that do cut:
AcuI ApoI AseI AvaI BanII BglI BmgBI BmrI
BsaI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspEI BspHI BsrDI BsrGI BstXI Bsu36I BtgI BtsI
CspCI EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI Hin4I
HincII MfeI MscI MslI MspA1I NcoI PasI PpuMI
SacI SacII SexAI SfcI SmaI SrfI StuI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AsiSI AvrII BaeI BamHI BanI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I
BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI
BsgI BsiEI BsiWI BsmBI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI ClaI
DraI DraIII DrdI EagI EarI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI