Restriction map for
pF1KB5905
(Linear) (Six-base) MAPSORT of: KIBB5905.seq Check: 467 from: 1 to: 1506
KIBB5905.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 925 1506
Size: 892 33 581
Fragments arranged by size:
892 581 33
AflIII A'CryG_T
Cuts at: 0 1196 1506
Size: 1196 310
AgeI A'CCGG_T
Cuts at: 0 1364 1506
Size: 1364 142
ApoI r'AATT_y
Cuts at: 0 1066 1506
Size: 1066 440
AvaI C'yCGr_G
Cuts at: 0 1109 1506
Size: 1109 397
AvrII C'CTAG_G
Cuts at: 0 213 1506
Size: 213 1293
BanI G'GyrC_C
Cuts at: 0 34 1117 1506
Size: 34 1083 389
Fragments arranged by size:
1083 389 34
BanII G_rGCy'C
Cuts at: 0 556 1425 1506
Size: 556 869 81
Fragments arranged by size:
869 556 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 1506
Size: 125 1381
BglII A'GATC_T
Cuts at: 0 993 1506
Size: 993 513
BlpI GC'TnA_GC
Cuts at: 0 602 1506
Size: 602 904
Bme1580I G_kGCm'C
Cuts at: 0 37 1295 1506
Size: 37 1258 211
Fragments arranged by size:
1258 211 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 813 1289 1506
Size: 813 476 217
Fragments arranged by size:
813 476 217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 198 1506
Size: 198 1308
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 1121 1506
Size: 536 585 385
Fragments arranged by size:
585 536 385
Bpu10I CC'TnA_GC
Cuts at: 0 555 1506
Size: 555 951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 950 1506
Size: 950 556
BsaBI GATnn'nnATC
Cuts at: 0 1242 1506
Size: 1242 264
BsaWI w'CCGG_w
Cuts at: 0 443 1364 1506
Size: 443 921 142
Fragments arranged by size:
921 443 142
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 315 345 1241 1271 1315 1345 1506
Size: 315 30 896 30 44 30 161
Fragments arranged by size:
896 315 161 44 30 30 30
BseYI C'CCAG_C
Cuts at: 0 1418 1506
Size: 1418 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 270 1506
Size: 35 235 1236
Fragments arranged by size:
1236 235 35
BsiHKAI G_wGCw'C
Cuts at: 0 556 1506
Size: 556 950
BsmI GAATG_Cn'
Cuts at: 0 286 1420 1506
Size: 286 1134 86
Fragments arranged by size:
1134 286 86
Bsp1286I G_dGCh'C
Cuts at: 0 37 556 1295 1425 1506
Size: 37 519 739 130 81
Fragments arranged by size:
739 519 130 81 37
BspHI T'CATG_A
Cuts at: 0 843 1281 1406 1506
Size: 843 438 125 100
Fragments arranged by size:
843 438 125 100
BsrDI GCAATG_nn'
Cuts at: 0 782 1506
Size: 782 724
BsrFI r'CCGG_y
Cuts at: 0 1364 1506
Size: 1364 142
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1367 1506
Size: 1367 139
BstYI r'GATC_y
Cuts at: 0 993 1506
Size: 993 513
BtsI GCAGTG_nn'
Cuts at: 0 325 1380 1506
Size: 325 1055 126
Fragments arranged by size:
1055 325 126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 91 126 1506
Size: 91 35 1380
Fragments arranged by size:
1380 91 35
EarI CTCTTCn'nnn_
Cuts at: 0 167 924 1506
Size: 167 757 582
Fragments arranged by size:
757 582 167
EcoICRI GAG'CTC
Cuts at: 0 554 1506
Size: 554 952
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 536 892 925 1121 1506
Size: 536 356 33 196 385
Fragments arranged by size:
536 385 356 196 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1222 1506
Size: 1222 284
EcoO109I rG'GnC_Cy
Cuts at: 0 809 1506
Size: 809 697
EcoRV GAT'ATC
Cuts at: 0 795 1506
Size: 795 711
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 557 589 880 912 1506
Size: 557 32 291 32 594
Fragments arranged by size:
594 557 291 32 32
HincII GTy'rAC
Cuts at: 0 129 1092 1506
Size: 129 963 414
Fragments arranged by size:
963 414 129
MfeI C'AATT_G
Cuts at: 0 275 980 1506
Size: 275 705 526
Fragments arranged by size:
705 526 275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1127 1506
Size: 1127 379
MslI CAynn'nnrTG
Cuts at: 0 848 1015 1506
Size: 848 167 491
Fragments arranged by size:
848 491 167
MspA1I CmG'CkG
Cuts at: 0 719 1506
Size: 719 787
NspI r_CATG'y
Cuts at: 0 1200 1506
Size: 1200 306
PciI A'CATG_T
Cuts at: 0 1196 1506
Size: 1196 310
PfoI T'CCnGG_A
Cuts at: 0 513 811 1506
Size: 513 298 695
Fragments arranged by size:
695 513 298
PpuMI rG'GwC_Cy
Cuts at: 0 809 1506
Size: 809 697
PspXI vC'TCGA_Gb
Cuts at: 0 1109 1506
Size: 1109 397
PstI C_TGCA'G
Cuts at: 0 792 1506
Size: 792 714
PvuII CAG'CTG
Cuts at: 0 719 1506
Size: 719 787
SacI G_AGCT'C
Cuts at: 0 556 1506
Size: 556 950
ScaI AGT'ACT
Cuts at: 0 963 1506
Size: 963 543
SfcI C'TryA_G
Cuts at: 0 402 596 788 1506
Size: 402 194 192 718
Fragments arranged by size:
718 402 194 192
SmlI C'TyrA_G
Cuts at: 0 965 1109 1506
Size: 965 144 397
Fragments arranged by size:
965 397 144
SspI AAT'ATT
Cuts at: 0 135 1506
Size: 135 1371
StuI AGG'CCT
Cuts at: 0 572 1506
Size: 572 934
StyI C'CwwG_G
Cuts at: 0 213 1506
Size: 213 1293
TatI w'GTAC_w
Cuts at: 0 58 961 1506
Size: 58 903 545
Fragments arranged by size:
903 545 58
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 60 92 240 272 1241 1273 1315 1347
Size: 60 32 148 32 969 32 42 32
Cuts at: 1347 1506
Size: 159
Fragments arranged by size:
969 159 148 60 42 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 124 807 1506
Size: 124 683 699
Fragments arranged by size:
699 683 124
XhoI C'TCGA_G
Cuts at: 0 1109 1506
Size: 1109 397
XmnI GAAnn'nnTTC
Cuts at: 0 694 1506
Size: 694 812
Enzymes that do cut:
AcuI AflIII AgeI ApoI AvaI AvrII BanI BanII
BbsI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaBI BsaWI BsaXI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BstXI BstYI BtsI CspCI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I HincII
MfeI MmeI MslI MspA1I NspI PciI PfoI PpuMI
PspXI PstI PvuII SacI ScaI SfcI SmlI SspI
StuI StyI TatI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BmgBI BmtI BsaI BsaAI BsaHI BseRI
BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI BtgZI
ClaI DraI DraIII DrdI EaeI EagI EciI EcoRI
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
TaqII XbaI XcmI XmaI ZraI