Restriction map for
pF1KB5880
(Linear) (Six-base) MAPSORT of: KIBB5880.seq Check: 2681 from: 1 to: 1488
KIBB5880.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 886 1488
Size: 886 602
AclI AA'CG_TT
Cuts at: 0 1271 1488
Size: 1271 217
AflIII A'CryG_T
Cuts at: 0 557 1488
Size: 557 931
AleI CACnn'nnGTG
Cuts at: 0 1153 1488
Size: 1153 335
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1488
Size: 1105 32 351
Fragments arranged by size:
1105 351 32
ApaLI G'TGCA_C
Cuts at: 0 1045 1488
Size: 1045 443
BamHI G'GATC_C
Cuts at: 0 263 1488
Size: 263 1225
BanI G'GyrC_C
Cuts at: 0 36 874 1488
Size: 36 838 614
Fragments arranged by size:
838 614 36
BanII G_rGCy'C
Cuts at: 0 446 600 1488
Size: 446 154 888
Fragments arranged by size:
888 446 154
BciVI GTATCCnnnnn_n'
Cuts at: 0 354 1488
Size: 354 1134
Bme1580I G_kGCm'C
Cuts at: 0 1049 1488
Size: 1049 439
BmrI ACTGGGnnnn_n'
Cuts at: 0 618 1488
Size: 618 870
BmtI G_CTAG'C
Cuts at: 0 338 1488
Size: 338 1150
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 312 794 1488
Size: 312 482 694
Fragments arranged by size:
694 482 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 764 838 1405 1488
Size: 176 588 74 567 83
Fragments arranged by size:
588 567 176 83 74
Bpu10I CC'TnA_GC
Cuts at: 0 940 1192 1488
Size: 940 252 296
Fragments arranged by size:
940 296 252
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 1378 1488
Size: 636 742 110
Fragments arranged by size:
742 636 110
BsaI GGTCTCn'nnnn_
Cuts at: 0 654 1488
Size: 654 834
BsaHI Gr'CG_yC
Cuts at: 0 1310 1488
Size: 1310 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1105 1135 1488
Size: 1105 30 353
Fragments arranged by size:
1105 353 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 343 1045 1120 1488
Size: 121 222 702 75 368
Fragments arranged by size:
702 368 222 121 75
BseYI C'CCAG_C
Cuts at: 0 381 687 1006 1450 1488
Size: 381 306 319 444 38
Fragments arranged by size:
444 381 319 306 38
BsiEI CG_ry'CG
Cuts at: 0 495 1488
Size: 495 993
BsiHKAI G_wGCw'C
Cuts at: 0 86 1049 1118 1488
Size: 86 963 69 370
Fragments arranged by size:
963 370 86 69
Bsp1286I G_dGCh'C
Cuts at: 0 86 446 600 1049 1118 1488
Size: 86 360 154 449 69 370
Fragments arranged by size:
449 370 360 154 86 69
BspHI T'CATG_A
Cuts at: 0 1094 1220 1301 1488
Size: 1094 126 81 187
Fragments arranged by size:
1094 187 126 81
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 321 872 886 1427 1488
Size: 321 551 14 541 61
Fragments arranged by size:
551 541 321 61 14
BsrFI r'CCGG_y
Cuts at: 0 33 1488
Size: 33 1455
BsrGI T'GTAC_A
Cuts at: 0 515 1488
Size: 515 973
BstAPI GCAn_nnn'nTGC
Cuts at: 0 95 1488
Size: 95 1393
BstXI CCAn_nnnn'nTGG
Cuts at: 0 324 614 1488
Size: 324 290 874
Fragments arranged by size:
874 324 290
BstYI r'GATC_y
Cuts at: 0 263 1359 1488
Size: 263 1096 129
Fragments arranged by size:
1096 263 129
BtgI C'CryG_G
Cuts at: 0 323 402 410 907 1488
Size: 323 79 8 497 581
Fragments arranged by size:
581 497 323 79 8
BtsI GCAGTG_nn'
Cuts at: 0 29 84 210 842 1488
Size: 29 55 126 632 646
Fragments arranged by size:
646 632 126 55 29
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 111 146 896 931 1488
Size: 111 35 750 35 557
Fragments arranged by size:
750 557 111 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1315 1488
Size: 1315 173
EaeI y'GGCC_r
Cuts at: 0 75 604 1488
Size: 75 529 884
Fragments arranged by size:
884 529 75
EarI CTCTTCn'nnn_
Cuts at: 0 1295 1488
Size: 1295 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 424 1488
Size: 19 405 1064
Fragments arranged by size:
1064 405 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 764 838 1405 1488
Size: 176 588 74 567 83
Fragments arranged by size:
588 567 176 83 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 1488
Size: 457 1031
EcoO109I rG'GnC_Cy
Cuts at: 0 310 1488
Size: 310 1178
HaeII r_GCGC'y
Cuts at: 0 683 1488
Size: 683 805
MfeI C'AATT_G
Cuts at: 0 1437 1488
Size: 1437 51
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1255 1452 1488
Size: 637 618 197 36
Fragments arranged by size:
637 618 197 36
MslI CAynn'nnrTG
Cuts at: 0 704 1153 1219 1488
Size: 704 449 66 269
Fragments arranged by size:
704 449 269 66
MspA1I CmG'CkG
Cuts at: 0 45 1006 1488
Size: 45 961 482
Fragments arranged by size:
961 482 45
NaeI GCC'GGC
Cuts at: 0 35 1488
Size: 35 1453
NcoI C'CATG_G
Cuts at: 0 323 402 1488
Size: 323 79 1086
Fragments arranged by size:
1086 323 79
NgoMIV G'CCGG_C
Cuts at: 0 33 1488
Size: 33 1455
NheI G'CTAG_C
Cuts at: 0 334 1488
Size: 334 1154
NspI r_CATG'y
Cuts at: 0 561 1488
Size: 561 927
PasI CC'CwG_GG
Cuts at: 0 459 1488
Size: 459 1029
PciI A'CATG_T
Cuts at: 0 557 1488
Size: 557 931
PfoI T'CCnGG_A
Cuts at: 0 266 1382 1488
Size: 266 1116 106
Fragments arranged by size:
1116 266 106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1488
Size: 1105 32 351
Fragments arranged by size:
1105 351 32
PpuMI rG'GwC_Cy
Cuts at: 0 310 1488
Size: 310 1178
PstI C_TGCA'G
Cuts at: 0 91 883 1424 1488
Size: 91 792 541 64
Fragments arranged by size:
792 541 91 64
PvuI CG_AT'CG
Cuts at: 0 495 1488
Size: 495 993
PvuII CAG'CTG
Cuts at: 0 1006 1488
Size: 1006 482
SbfI CC_TGCA'GG
Cuts at: 0 883 1488
Size: 883 605
SexAI A'CCwGG_T
Cuts at: 0 1060 1488
Size: 1060 428
SfcI C'TryA_G
Cuts at: 0 87 879 1420 1488
Size: 87 792 541 68
Fragments arranged by size:
792 541 87 68
SmlI C'TyrA_G
Cuts at: 0 651 1393 1488
Size: 651 742 95
Fragments arranged by size:
742 651 95
StyI C'CwwG_G
Cuts at: 0 323 402 1488
Size: 323 79 1086
Fragments arranged by size:
1086 323 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 415 884 1294 1488
Size: 415 469 410 194
Fragments arranged by size:
469 415 410 194
TatI w'GTAC_w
Cuts at: 0 515 1488
Size: 515 973
XbaI T'CTAG_A
Cuts at: 0 1293 1488
Size: 1293 195
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 631 1488
Size: 631 857
Enzymes that do cut:
AarI AclI AflIII AleI AloI ApaLI BamHI BanI
BanII BciVI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrFI BsrGI BstAPI BstXI BstYI
BtgI BtsI CspCI DrdI EaeI EarI EciI Eco57MI
EcoNI EcoO109I HaeII MfeI MmeI MslI MspA1I NaeI
NcoI NgoMIV NheI NspI PasI PciI PfoI PpiI
PpuMI PstI PvuI PvuII SbfI SexAI SfcI SmlI
StyI TaqII TatI XbaI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AcuI AfeI AflII AgeI AhdI
AlfI AlwNI ApaI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BbvCI BcgI BclI BfrBI
BglI BglII BlpI BmgBI BsaAI BsaBI BsaWI BsgI
BsiWI BsmI BsmBI BspEI BsrBI BsrDI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII
EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KasI KpnI MluI MscI
NarI NdeI NotI NruI NsiI PacI PflMI PmeI
PmlI PshAI PsiI PspOMI PspXI PsrI RsrII SacI
SacII SalI SanDI SapI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XhoI XmaI XmnI ZraI