Restriction map for
pF1KB5876
(Linear) (Six-base) MAPSORT of: KIBB5876.seq Check: 3312 from: 1 to: 1485
KIBB5876.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AclI AA'CG_TT
Cuts at: 0 1327 1485
Size: 1327 158
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 1175 1485
Size: 444 731 310
Fragments arranged by size:
731 444 310
AflIII A'CryG_T
Cuts at: 0 575 1485
Size: 575 910
AhdI GACnn_n'nnGTC
Cuts at: 0 285 1485
Size: 285 1200
AleI CACnn'nnGTG
Cuts at: 0 947 1485
Size: 947 538
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1335 1367 1485
Size: 1335 32 118
Fragments arranged by size:
1335 118 32
AlwNI CAG_nnn'CTG
Cuts at: 0 928 1485
Size: 928 557
ApaI G_GGCC'C
Cuts at: 0 77 1485
Size: 77 1408
ApoI r'AATT_y
Cuts at: 0 330 417 448 1239 1485
Size: 330 87 31 791 246
Fragments arranged by size:
791 330 246 87 31
AvaI C'yCGr_G
Cuts at: 0 52 223 259 1485
Size: 52 171 36 1226
Fragments arranged by size:
1226 171 52 36
BanII G_rGCy'C
Cuts at: 0 77 231 1485
Size: 77 154 1254
Fragments arranged by size:
1254 154 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 359 1485
Size: 359 1126
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1402 1436 1485
Size: 1402 34 49
Fragments arranged by size:
1402 49 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1449 1485
Size: 1449 36
BfrBI ATG'CAT
Cuts at: 0 660 1485
Size: 660 825
BlpI GC'TnA_GC
Cuts at: 0 5 1485
Size: 5 1480
Bme1580I G_kGCm'C
Cuts at: 0 77 1485
Size: 77 1408
BmrI ACTGGGnnnn_n'
Cuts at: 0 207 279 467 1485
Size: 207 72 188 1018
Fragments arranged by size:
1018 207 188 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 1485
Size: 680 805
Bpu10I CC'TnA_GC
Cuts at: 0 79 1485
Size: 79 1406
BsaHI Gr'CG_yC
Cuts at: 0 245 281 1485
Size: 245 36 1204
Fragments arranged by size:
1204 245 36
BsaWI w'CCGG_w
Cuts at: 0 972 1380 1485
Size: 972 408 105
Fragments arranged by size:
972 408 105
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 199 229 1485
Size: 199 30 1256
Fragments arranged by size:
1256 199 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 812 1048 1485
Size: 812 236 437
Fragments arranged by size:
812 437 236
BseYI C'CCAG_C
Cuts at: 0 313 1485
Size: 313 1172
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 1485
Size: 605 880
BsiHKAI G_wGCw'C
Cuts at: 0 1200 1485
Size: 1200 285
BsmBI CGTCTCn'nnnn_
Cuts at: 0 53 1485
Size: 53 1432
Bsp1286I G_dGCh'C
Cuts at: 0 77 231 1200 1485
Size: 77 154 969 285
Fragments arranged by size:
969 285 154 77
BspEI T'CCGG_A
Cuts at: 0 1380 1485
Size: 1380 105
BsrDI GCAATG_nn'
Cuts at: 0 128 1485
Size: 128 1357
BssSI C'ACGA_G
Cuts at: 0 625 1485
Size: 625 860
BstBI TT'CG_AA
Cuts at: 0 1334 1485
Size: 1334 151
BstYI r'GATC_y
Cuts at: 0 987 1485
Size: 987 498
BtgI C'CryG_G
Cuts at: 0 97 203 1485
Size: 97 106 1282
Fragments arranged by size:
1282 106 97
BtsI GCAGTG_nn'
Cuts at: 0 86 1200 1420 1485
Size: 86 1114 220 65
Fragments arranged by size:
1114 220 86 65
DraI TTT'AAA
Cuts at: 0 549 1485
Size: 549 936
DraIII CAC_nnn'GTG
Cuts at: 0 350 1485
Size: 350 1135
EaeI y'GGCC_r
Cuts at: 0 318 749 1485
Size: 318 431 736
Fragments arranged by size:
736 431 318
EarI CTCTTCn'nnn_
Cuts at: 0 787 1485
Size: 787 698
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 221 1485
Size: 221 1264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 444 680 1175 1485
Size: 444 236 495 310
Fragments arranged by size:
495 444 310 236
EcoO109I rG'GnC_Cy
Cuts at: 0 73 74 1485
Size: 73 1 1411
Fragments arranged by size:
1411 73 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1293 1485
Size: 1293 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 199 231 271 303 858 890 1006 1038
Size: 199 32 40 32 555 32 116 32
Cuts at: 1038 1197 1229 1485
Size: 159 32 256
Fragments arranged by size:
555 256 199 159 116 40 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1275 1485
Size: 1275 210
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1485
Size: 766 719
MscI TGG'CCA
Cuts at: 0 751 1485
Size: 751 734
MslI CAynn'nnrTG
Cuts at: 0 947 1485
Size: 947 538
MspA1I CmG'CkG
Cuts at: 0 99 317 1485
Size: 99 218 1168
Fragments arranged by size:
1168 218 99
NcoI C'CATG_G
Cuts at: 0 203 1485
Size: 203 1282
NsiI A_TGCA'T
Cuts at: 0 662 1485
Size: 662 823
NspI r_CATG'y
Cuts at: 0 46 579 664 1485
Size: 46 533 85 821
Fragments arranged by size:
821 533 85 46
PciI A'CATG_T
Cuts at: 0 575 1485
Size: 575 910
PflMI CCAn_nnn'nTGG
Cuts at: 0 350 1485
Size: 350 1135
PspOMI G'GGCC_C
Cuts at: 0 73 1485
Size: 73 1412
PstI C_TGCA'G
Cuts at: 0 411 1485
Size: 411 1074
SacII CC_GC'GG
Cuts at: 0 100 1485
Size: 100 1385
SexAI A'CCwGG_T
Cuts at: 0 1178 1485
Size: 1178 307
SfcI C'TryA_G
Cuts at: 0 407 1485
Size: 407 1078
SmaI CCC'GGG
Cuts at: 0 225 261 1485
Size: 225 36 1224
Fragments arranged by size:
1224 225 36
SpeI A'CTAG_T
Cuts at: 0 13 1485
Size: 13 1472
SphI G_CATG'C
Cuts at: 0 46 664 1485
Size: 46 618 821
Fragments arranged by size:
821 618 46
SspI AAT'ATT
Cuts at: 0 756 1485
Size: 756 729
StyI C'CwwG_G
Cuts at: 0 203 325 1061 1485
Size: 203 122 736 424
Fragments arranged by size:
736 424 203 122
XbaI T'CTAG_A
Cuts at: 0 910 1485
Size: 910 575
XmaI C'CCGG_G
Cuts at: 0 223 259 1485
Size: 223 36 1226
Fragments arranged by size:
1226 223 36
XmnI GAAnn'nnTTC
Cuts at: 0 397 1242 1370 1485
Size: 397 845 128 115
Fragments arranged by size:
845 397 128 115
Enzymes that do cut:
AclI AcuI AflIII AhdI AleI AloI AlwNI ApaI
ApoI AvaI BanII BbsI BcgI BciVI BfrBI BlpI
Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BsrDI BssSI
BstBI BstYI BtgI BtsI DraI DraIII EaeI EarI
EciI Eco57MI EcoO109I FalI Hin4I HindIII MmeI MscI
MslI MspA1I NcoI NsiI NspI PciI PflMI PspOMI
PstI SacII SexAI SfcI SmaI SpeI SphI SspI
StyI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BbvCI BclI BglI BglII BmgBI BmtI BplI
BpuEI BsaI BsaAI BsaBI BsiEI BsiWI BsmI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmlI SnaBI SrfI StuI
SwaI TaqII TatI TstI Tth111I XcmI XhoI ZraI