Restriction map for
pF1KB5858
(Linear) (Six-base) MAPSORT of: KIBB5858.seq Check: 3435 from: 1 to: 1470
KIBB5858.orf
With 182 enzymes: SgfI *
October 7, 2009 14:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 169 238 1470
Size: 169 69 1232
Fragments arranged by size:
1232 169 69
AclI AA'CG_TT
Cuts at: 0 770 1470
Size: 770 700
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 817 868 1470
Size: 82 735 51 602
Fragments arranged by size:
735 602 82 51
AflIII A'CryG_T
Cuts at: 0 823 1470
Size: 823 647
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1470
Size: 1096 32 342
Fragments arranged by size:
1096 342 32
ApaI G_GGCC'C
Cuts at: 0 714 1470
Size: 714 756
ApoI r'AATT_y
Cuts at: 0 1363 1470
Size: 1363 107
AvrII C'CTAG_G
Cuts at: 0 714 1470
Size: 714 756
BanI G'GyrC_C
Cuts at: 0 294 352 1470
Size: 294 58 1118
Fragments arranged by size:
1118 294 58
BanII G_rGCy'C
Cuts at: 0 362 714 1430 1470
Size: 362 352 716 40
Fragments arranged by size:
716 362 352 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 24 1470
Size: 24 1446
BbvCI CC'TCA_GC
Cuts at: 0 41 1470
Size: 41 1429
BciVI GTATCCnnnnn_n'
Cuts at: 0 924 1470
Size: 924 546
BclI T'GATC_A
Cuts at: 0 555 1470
Size: 555 915
BglII A'GATC_T
Cuts at: 0 722 1470
Size: 722 748
BlpI GC'TnA_GC
Cuts at: 0 104 1060 1470
Size: 104 956 410
Fragments arranged by size:
956 410 104
Bme1580I G_kGCm'C
Cuts at: 0 299 714 1238 1470
Size: 299 415 524 232
Fragments arranged by size:
524 415 299 232
BmgBI CAC'GTC
Cuts at: 0 450 1470
Size: 450 1020
BmrI ACTGGGnnnn_n'
Cuts at: 0 1183 1470
Size: 1183 287
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1125 1470
Size: 1125 345
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1075 1470
Size: 1027 48 395
Fragments arranged by size:
1027 395 48
Bpu10I CC'TnA_GC
Cuts at: 0 41 1470
Size: 41 1429
BsaAI yAC'GTr
Cuts at: 0 405 1470
Size: 405 1065
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1009 1039 1096 1126 1270 1300 1470
Size: 1009 30 57 30 144 30 170
Fragments arranged by size:
1009 170 144 57 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 436 439 751 1117 1470
Size: 436 3 312 366 353
Fragments arranged by size:
436 366 353 312 3
BseYI C'CCAG_C
Cuts at: 0 1429 1446 1470
Size: 1429 17 24
Fragments arranged by size:
1429 24 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 804 1345 1470
Size: 804 541 125
Fragments arranged by size:
804 541 125
BsiEI CG_ry'CG
Cuts at: 0 225 1470
Size: 225 1245
BsiHKAI G_wGCw'C
Cuts at: 0 413 1470
Size: 413 1057
Bsp1286I G_dGCh'C
Cuts at: 0 299 362 413 714 1238 1430 1470
Size: 299 63 51 301 524 192 40
Fragments arranged by size:
524 301 299 192 63 51 40
BspHI T'CATG_A
Cuts at: 0 660 975 1470
Size: 660 315 495
Fragments arranged by size:
660 495 315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 169 238 446 1404 1470
Size: 169 69 208 958 66
Fragments arranged by size:
958 208 169 69 66
BsrBI CCG'CTC
Cuts at: 0 108 1470
Size: 108 1362
BsrDI GCAATG_nn'
Cuts at: 0 1440 1470
Size: 1440 30
BssHII G'CGCG_C
Cuts at: 0 322 1470
Size: 322 1148
BstXI CCAn_nnnn'nTGG
Cuts at: 0 193 1128 1269 1470
Size: 193 935 141 201
Fragments arranged by size:
935 201 193 141
BstYI r'GATC_y
Cuts at: 0 722 1470
Size: 722 748
BtgI C'CryG_G
Cuts at: 0 383 575 1470
Size: 383 192 895
Fragments arranged by size:
895 383 192
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 172 1470
Size: 172 1298
BtsI GCAGTG_nn'
Cuts at: 0 128 205 674 831 1470
Size: 128 77 469 157 639
Fragments arranged by size:
639 469 157 128 77
EaeI y'GGCC_r
Cuts at: 0 272 472 1181 1421 1470
Size: 272 200 709 240 49
Fragments arranged by size:
709 272 240 200 49
EarI CTCTTCn'nnn_
Cuts at: 0 865 1470
Size: 865 605
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1342 1470
Size: 1342 128
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 817 868 1027 1075 1470
Size: 82 735 51 159 48 395
Fragments arranged by size:
735 395 159 82 51 48
EcoO109I rG'GnC_Cy
Cuts at: 0 435 711 1077 1470
Size: 435 276 366 393
Fragments arranged by size:
435 393 366 276
EcoRI G'AATT_C
Cuts at: 0 1363 1470
Size: 1363 107
EcoRV GAT'ATC
Cuts at: 0 93 1470
Size: 93 1377
MslI CAynn'nnrTG
Cuts at: 0 191 260 1045 1183 1228 1321 1470
Size: 191 69 785 138 45 93 149
Fragments arranged by size:
785 191 149 138 93 69 45
MspA1I CmG'CkG
Cuts at: 0 40 795 1470
Size: 40 755 675
Fragments arranged by size:
755 675 40
NspI r_CATG'y
Cuts at: 0 501 827 1050 1216 1350 1470
Size: 501 326 223 166 134 120
Fragments arranged by size:
501 326 223 166 134 120
PciI A'CATG_T
Cuts at: 0 823 1470
Size: 823 647
PmlI CAC'GTG
Cuts at: 0 405 1470
Size: 405 1065
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 1470
Size: 1096 32 342
Fragments arranged by size:
1096 342 32
PpuMI rG'GwC_Cy
Cuts at: 0 435 1077 1470
Size: 435 642 393
Fragments arranged by size:
642 435 393
PspOMI G'GGCC_C
Cuts at: 0 710 1470
Size: 710 760
PstI C_TGCA'G
Cuts at: 0 90 572 1470
Size: 90 482 898
Fragments arranged by size:
898 482 90
PvuII CAG'CTG
Cuts at: 0 795 1470
Size: 795 675
SbfI CC_TGCA'GG
Cuts at: 0 572 1470
Size: 572 898
ScaI AGT'ACT
Cuts at: 0 469 808 1470
Size: 469 339 662
Fragments arranged by size:
662 469 339
SfcI C'TryA_G
Cuts at: 0 86 568 1470
Size: 86 482 902
Fragments arranged by size:
902 482 86
SphI G_CATG'C
Cuts at: 0 501 1216 1470
Size: 501 715 254
Fragments arranged by size:
715 501 254
StuI AGG'CCT
Cuts at: 0 566 1096 1108 1470
Size: 566 530 12 362
Fragments arranged by size:
566 530 362 12
StyI C'CwwG_G
Cuts at: 0 71 714 926 1470
Size: 71 643 212 544
Fragments arranged by size:
643 544 212 71
TatI w'GTAC_w
Cuts at: 0 467 503 634 806 1370 1470
Size: 467 36 131 172 564 100
Fragments arranged by size:
564 467 172 131 100 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1470
Size: 1270 32 168
Fragments arranged by size:
1270 168 32
Tth111I GACn'n_nGTC
Cuts at: 0 747 1470
Size: 747 723
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 603 933 1128 1416 1470
Size: 603 330 195 288 54
Fragments arranged by size:
603 330 288 195 54
XmnI GAAnn'nnTTC
Cuts at: 0 1363 1470
Size: 1363 107
Enzymes that do cut:
AarI AclI AcuI AflIII AloI ApaI ApoI AvrII
BanI BanII BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BspMI
BsrBI BsrDI BssHII BstXI BstYI BtgI BtgZI BtsI
EaeI EarI EciI Eco57MI EcoO109I EcoRI EcoRV MslI
MspA1I NspI PciI PmlI PpiI PpuMI PspOMI PstI
PvuII SbfI ScaI SfcI SphI StuI StyI TatI
TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AhdI AleI
AlfI AlwNI ApaLI AscI AseI AsiSI AvaI BaeI
BamHI BbeI BcgI BfrBI BglI BmtI BpuEI BsaI
BsaBI BsaHI BsaWI BsiWI BsmI BsmBI BspEI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI SwaI TaqII XbaI XhoI XmaI ZraI