Restriction map for
pF1KB5842
(Linear) (Six-base) MAPSORT of: KIBB5842.seq Check: 4237 from: 1 to: 1449
KIBB5842.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 937 979 1449
Size: 499 438 42 470
Fragments arranged by size:
499 470 438 42
ApaLI G'TGCA_C
Cuts at: 0 646 1449
Size: 646 803
ApoI r'AATT_y
Cuts at: 0 329 545 864 1449
Size: 329 216 319 585
Fragments arranged by size:
585 329 319 216
AvaI C'yCGr_G
Cuts at: 0 1184 1449
Size: 1184 265
BanI G'GyrC_C
Cuts at: 0 442 1449
Size: 442 1007
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1096 1130 1449
Size: 1096 34 319
Fragments arranged by size:
1096 319 34
BclI T'GATC_A
Cuts at: 0 711 1310 1449
Size: 711 599 139
Fragments arranged by size:
711 599 139
BlpI GC'TnA_GC
Cuts at: 0 1296 1449
Size: 1296 153
Bme1580I G_kGCm'C
Cuts at: 0 106 650 1449
Size: 106 544 799
Fragments arranged by size:
799 544 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 924 1078 1449
Size: 924 154 371
Fragments arranged by size:
924 371 154
BmtI G_CTAG'C
Cuts at: 0 1051 1449
Size: 1051 398
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 972 1449
Size: 113 859 477
Fragments arranged by size:
859 477 113
BsaI GGTCTCn'nnnn_
Cuts at: 0 1240 1449
Size: 1240 209
BsaAI yAC'GTr
Cuts at: 0 651 1449
Size: 651 798
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 110 385 1017 1363 1449
Size: 110 275 632 346 86
Fragments arranged by size:
632 346 275 110 86
BseYI C'CCAG_C
Cuts at: 0 344 1356 1449
Size: 344 1012 93
Fragments arranged by size:
1012 344 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1449
Size: 790 659
BsiHKAI G_wGCw'C
Cuts at: 0 7 650 1449
Size: 7 643 799
Fragments arranged by size:
799 643 7
BsmI GAATG_Cn'
Cuts at: 0 377 1449
Size: 377 1072
Bsp1286I G_dGCh'C
Cuts at: 0 7 106 650 1449
Size: 7 99 544 799
Fragments arranged by size:
799 544 99 7
BspHI T'CATG_A
Cuts at: 0 860 1131 1449
Size: 860 271 318
Fragments arranged by size:
860 318 271
BsrBI CCG'CTC
Cuts at: 0 440 1449
Size: 440 1009
BsrDI GCAATG_nn'
Cuts at: 0 383 506 1449
Size: 383 123 943
Fragments arranged by size:
943 383 123
BsrGI T'GTAC_A
Cuts at: 0 849 1449
Size: 849 600
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 1264 1449
Size: 588 676 185
Fragments arranged by size:
676 588 185
BstYI r'GATC_y
Cuts at: 0 243 681 1449
Size: 243 438 768
Fragments arranged by size:
768 438 243
BtsI GCAGTG_nn'
Cuts at: 0 966 1449
Size: 966 483
DraI TTT'AAA
Cuts at: 0 227 1449
Size: 227 1222
DraIII CAC_nnn'GTG
Cuts at: 0 654 1449
Size: 654 795
EaeI y'GGCC_r
Cuts at: 0 8 1449
Size: 8 1441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 499 937 972 979 1449
Size: 113 386 438 35 7 470
Fragments arranged by size:
470 438 386 113 35 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 95 481 901 1449
Size: 95 386 420 548
Fragments arranged by size:
548 420 386 95
EcoO109I rG'GnC_Cy
Cuts at: 0 67 1449
Size: 67 1382
EcoRI G'AATT_C
Cuts at: 0 864 1449
Size: 864 585
EcoRV GAT'ATC
Cuts at: 0 405 1449
Size: 405 1044
HaeII r_GCGC'y
Cuts at: 0 957 1449
Size: 957 492
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 1449
Size: 256 32 1161
Fragments arranged by size:
1161 256 32
MspA1I CmG'CkG
Cuts at: 0 60 132 1356 1449
Size: 60 72 1224 93
Fragments arranged by size:
1224 93 72 60
NdeI CA'TA_TG
Cuts at: 0 942 1317 1449
Size: 942 375 132
Fragments arranged by size:
942 375 132
NheI G'CTAG_C
Cuts at: 0 1047 1449
Size: 1047 402
PasI CC'CwG_GG
Cuts at: 0 667 1449
Size: 667 782
PfoI T'CCnGG_A
Cuts at: 0 677 1449
Size: 677 772
PmlI CAC'GTG
Cuts at: 0 651 1449
Size: 651 798
PsiI TTA'TAA
Cuts at: 0 31 1449
Size: 31 1418
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 515 547 1449
Size: 515 32 902
Fragments arranged by size:
902 515 32
PvuII CAG'CTG
Cuts at: 0 60 132 1356 1449
Size: 60 72 1224 93
Fragments arranged by size:
1224 93 72 60
ScaI AGT'ACT
Cuts at: 0 934 1449
Size: 934 515
SexAI A'CCwGG_T
Cuts at: 0 201 1449
Size: 201 1248
SfcI C'TryA_G
Cuts at: 0 532 1430 1449
Size: 532 898 19
Fragments arranged by size:
898 532 19
TatI w'GTAC_w
Cuts at: 0 849 932 963 1449
Size: 849 83 31 486
Fragments arranged by size:
849 486 83 31
Enzymes that do cut:
AcuI ApaLI ApoI AvaI BanI BcgI BclI BlpI
Bme1580I BmrI BmtI BpmI BsaI BsaAI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI BsrGI
BstXI BstYI BtsI DraI DraIII EaeI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I MspA1I NdeI NheI
PasI PfoI PmlI PsiI PsrI PvuII ScaI SexAI
SfcI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BciVI BfrBI BglI BglII BmgBI BplI Bpu10I
BpuEI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI
EarI EciI EcoICRI FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI NaeI NarI NcoI NgoMIV NotI NruI NsiI
NspI PacI PciI PflMI PmeI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TaqII TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI