Restriction map for
pF1KB5841
(Linear) (Six-base) MAPSORT of: KIBB5841.seq Check: 2993 from: 1 to: 1209
KIBB5841.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 876 1209
Size: 876 333
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 428 484 1027 1209
Size: 243 185 56 543 182
Fragments arranged by size:
543 243 185 182 56
AfeI AGC'GCT
Cuts at: 0 502 1209
Size: 502 707
AhdI GACnn_n'nnGTC
Cuts at: 0 558 1209
Size: 558 651
AleI CACnn'nnGTG
Cuts at: 0 453 1209
Size: 453 756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1123 1209
Size: 1123 86
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 274 306 643 675 1209
Size: 274 32 337 32 534
Fragments arranged by size:
534 337 274 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 832 1209
Size: 832 377
ApaI G_GGCC'C
Cuts at: 0 92 631 826 1209
Size: 92 539 195 383
Fragments arranged by size:
539 383 195 92
ApaLI G'TGCA_C
Cuts at: 0 437 1209
Size: 437 772
AscI GG'CGCG_CC
Cuts at: 0 430 1209
Size: 430 779
AvaI C'yCGr_G
Cuts at: 0 508 1209
Size: 508 701
BanI G'GyrC_C
Cuts at: 0 250 389 547 723 807 1209
Size: 250 139 158 176 84 402
Fragments arranged by size:
402 250 176 158 139 84
BanII G_rGCy'C
Cuts at: 0 92 356 631 826 1076 1209
Size: 92 264 275 195 250 133
Fragments arranged by size:
275 264 250 195 133 92
BbeI G_GCGC'C
Cuts at: 0 551 811 1209
Size: 551 260 398
Fragments arranged by size:
551 398 260
BglI GCCn_nnn'nGGC
Cuts at: 0 1150 1209
Size: 1150 59
Bme1580I G_kGCm'C
Cuts at: 0 92 392 441 631 826 1200 1209
Size: 92 300 49 190 195 374 9
Fragments arranged by size:
374 300 195 190 92 49 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 468 1209
Size: 468 741
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 499 517 1204 1209
Size: 463 36 18 687 5
Fragments arranged by size:
687 463 36 18 5
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 295 1209
Size: 139 156 914
Fragments arranged by size:
914 156 139
BsaAI yAC'GTr
Cuts at: 0 177 241 597 639 768 930 1209
Size: 177 64 356 42 129 162 279
Fragments arranged by size:
356 279 177 162 129 64 42
BsaHI Gr'CG_yC
Cuts at: 0 548 680 808 1209
Size: 548 132 128 401
Fragments arranged by size:
548 401 132 128
BsaWI w'CCGG_w
Cuts at: 0 272 1209
Size: 272 937
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 274 304 476 506 1209
Size: 274 30 172 30 703
Fragments arranged by size:
703 274 172 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 1209
Size: 439 770
BseYI C'CCAG_C
Cuts at: 0 535 951 1124 1209
Size: 535 416 173 85
Fragments arranged by size:
535 416 173 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 825 1209
Size: 818 7 384
Fragments arranged by size:
818 384 7
BsiEI CG_ry'CG
Cuts at: 0 368 1209
Size: 368 841
BsiHKAI G_wGCw'C
Cuts at: 0 356 441 1208 1209
Size: 356 85 767 1
Fragments arranged by size:
767 356 85 1
Bsp1286I G_dGCh'C
Cuts at: 0 92 356 392 441 631 826 1076 1200
Size: 92 264 36 49 190 195 250 124
Cuts at: 1200 1208 1209
Size: 8 1
Fragments arranged by size:
264 250 195 190 124 92 49 36
8 1
BspEI T'CCGG_A
Cuts at: 0 272 1209
Size: 272 937
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 530 876 1209
Size: 530 346 333
Fragments arranged by size:
530 346 333
BsrBI CCG'CTC
Cuts at: 0 817 1209
Size: 817 392
BsrFI r'CCGG_y
Cuts at: 0 473 1209
Size: 473 736
BssHII G'CGCG_C
Cuts at: 0 430 1209
Size: 430 779
BssSI C'ACGA_G
Cuts at: 0 453 1209
Size: 453 756
BstEII G'GTnAC_C
Cuts at: 0 564 1209
Size: 564 645
Bsu36I CC'TnA_GG
Cuts at: 0 13 38 1209
Size: 13 25 1171
Fragments arranged by size:
1171 25 13
BtgI C'CryG_G
Cuts at: 0 683 1209
Size: 683 526
BtsI GCAGTG_nn'
Cuts at: 0 895 1209
Size: 895 314
ClaI AT'CG_AT
Cuts at: 0 644 1209
Size: 644 565
DraIII CAC_nnn'GTG
Cuts at: 0 25 1209
Size: 25 1184
DrdI GACnn_nn'nnGTC
Cuts at: 0 685 1209
Size: 685 524
EaeI y'GGCC_r
Cuts at: 0 416 994 1209
Size: 416 578 215
Fragments arranged by size:
578 416 215
EarI CTCTTCn'nnn_
Cuts at: 0 914 1138 1183 1209
Size: 914 224 45 26
Fragments arranged by size:
914 224 45 26
EcoICRI GAG'CTC
Cuts at: 0 354 1209
Size: 354 855
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 243 428 463 484 499 517 1027 1204
Size: 243 185 35 21 15 18 510 177
Cuts at: 1204 1209
Size: 5
Fragments arranged by size:
510 243 185 177 35 21 18 15
5
EcoO109I rG'GnC_Cy
Cuts at: 0 88 89 627 628 829 1209
Size: 88 1 538 1 201 380
Fragments arranged by size:
538 380 201 88 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 207 1209
Size: 207 1002
HaeII r_GCGC'y
Cuts at: 0 504 551 721 811 1209
Size: 504 47 170 90 398
Fragments arranged by size:
504 398 170 90 47
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 544 576 1209
Size: 544 32 633
Fragments arranged by size:
633 544 32
HincII GTy'rAC
Cuts at: 0 690 960 1209
Size: 690 270 249
Fragments arranged by size:
690 270 249
HindIII A'AGCT_T
Cuts at: 0 1039 1209
Size: 1039 170
KasI G'GCGC_C
Cuts at: 0 547 807 1209
Size: 547 260 402
Fragments arranged by size:
547 402 260
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1038 1209
Size: 1038 171
MscI TGG'CCA
Cuts at: 0 418 996 1209
Size: 418 578 213
Fragments arranged by size:
578 418 213
MslI CAynn'nnrTG
Cuts at: 0 62 453 1209
Size: 62 391 756
Fragments arranged by size:
756 391 62
MspA1I CmG'CkG
Cuts at: 0 539 861 955 1209
Size: 539 322 94 254
Fragments arranged by size:
539 322 254 94
NaeI GCC'GGC
Cuts at: 0 475 1209
Size: 475 734
NarI GG'CG_CC
Cuts at: 0 548 808 1209
Size: 548 260 401
Fragments arranged by size:
548 401 260
NgoMIV G'CCGG_C
Cuts at: 0 473 1209
Size: 473 736
PasI CC'CwG_GG
Cuts at: 0 227 1209
Size: 227 982
PflMI CCAn_nnn'nTGG
Cuts at: 0 177 978 1057 1209
Size: 177 801 79 152
Fragments arranged by size:
801 177 152 79
PfoI T'CCnGG_A
Cuts at: 0 494 1209
Size: 494 715
PmlI CAC'GTG
Cuts at: 0 177 241 597 1209
Size: 177 64 356 612
Fragments arranged by size:
612 356 177 64
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 274 306 1209
Size: 274 32 903
Fragments arranged by size:
903 274 32
PpuMI rG'GwC_Cy
Cuts at: 0 829 1209
Size: 829 380
PspOMI G'GGCC_C
Cuts at: 0 88 627 822 1209
Size: 88 539 195 387
Fragments arranged by size:
539 387 195 88
PstI C_TGCA'G
Cuts at: 0 131 1209
Size: 131 1078
PvuII CAG'CTG
Cuts at: 0 955 1209
Size: 955 254
SacI G_AGCT'C
Cuts at: 0 356 1209
Size: 356 853
SbfI CC_TGCA'GG
Cuts at: 0 131 1209
Size: 131 1078
SfcI C'TryA_G
Cuts at: 0 127 1171 1209
Size: 127 1044 38
Fragments arranged by size:
1044 127 38
SfoI GGC'GCC
Cuts at: 0 549 809 1209
Size: 549 260 400
Fragments arranged by size:
549 400 260
StuI AGG'CCT
Cuts at: 0 125 1209
Size: 125 1084
StyI C'CwwG_G
Cuts at: 0 305 1209
Size: 305 904
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 268 382 552 792 1209
Size: 268 114 170 240 417
Fragments arranged by size:
417 268 240 170 114
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 11 43 476 508 1209
Size: 11 32 433 32 701
Fragments arranged by size:
701 433 32 32 11
XbaI T'CTAG_A
Cuts at: 0 75 1209
Size: 75 1134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 1146 1209
Size: 717 429 63
Fragments arranged by size:
717 429 63
XmnI GAAnn'nnTTC
Cuts at: 0 331 1209
Size: 331 878
Enzymes that do cut:
AarI AcuI AfeI AhdI AleI AlfI AloI AlwNI
ApaI ApaLI AscI AvaI BanI BanII BbeI BglI
Bme1580I BplI BpmI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI
BsrBI BsrFI BssHII BssSI BstEII Bsu36I BtgI BtsI
ClaI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV PasI PflMI PfoI
PmlI PpiI PpuMI PspOMI PstI PvuII SacI SbfI
SfcI SfoI StuI StyI TaqII TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI ApoI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
Bpu10I BpuEI BsaBI BsiWI BsmI BsmBI BspHI BsrDI
BsrGI BstAPI BstBI BstXI BstYI BstZ17I BtgZI CspCI
DraI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XhoI XmaI ZraI