Restriction map for
pF1KB5840
(Linear) (Six-base) MAPSORT of: KIBB5840.seq Check: 681 from: 1 to: 1446
KIBB5840.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 187 1351 1446
Size: 187 1164 95
Fragments arranged by size:
1164 187 95
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1409 1446
Size: 581 828 37
Fragments arranged by size:
828 581 37
AflIII A'CryG_T
Cuts at: 0 710 1446
Size: 710 736
AlwNI CAG_nnn'CTG
Cuts at: 0 729 1387 1446
Size: 729 658 59
Fragments arranged by size:
729 658 59
ApaI G_GGCC'C
Cuts at: 0 1239 1446
Size: 1239 207
ApoI r'AATT_y
Cuts at: 0 456 1446
Size: 456 990
BamHI G'GATC_C
Cuts at: 0 899 1446
Size: 899 547
BanII G_rGCy'C
Cuts at: 0 580 806 1239 1446
Size: 580 226 433 207
Fragments arranged by size:
580 433 226 207
BfrBI ATG'CAT
Cuts at: 0 195 1446
Size: 195 1251
BlpI GC'TnA_GC
Cuts at: 0 959 1446
Size: 959 487
Bme1580I G_kGCm'C
Cuts at: 0 643 1239 1446
Size: 643 596 207
Fragments arranged by size:
643 596 207
BmgBI CAC'GTC
Cuts at: 0 1129 1446
Size: 1129 317
BmrI ACTGGGnnnn_n'
Cuts at: 0 504 875 1047 1446
Size: 504 371 172 399
Fragments arranged by size:
504 399 371 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 916 1446
Size: 378 538 530
Fragments arranged by size:
538 530 378
BsaI GGTCTCn'nnnn_
Cuts at: 0 296 1446
Size: 296 1150
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 966 1060 1207 1446
Size: 966 94 147 239
Fragments arranged by size:
966 239 147 94
BseYI C'CCAG_C
Cuts at: 0 1329 1446
Size: 1329 117
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 838 1128 1446
Size: 540 298 290 318
Fragments arranged by size:
540 318 298 290
BsiHKAI G_wGCw'C
Cuts at: 0 806 1446
Size: 806 640
BsmI GAATG_Cn'
Cuts at: 0 507 938 1003 1446
Size: 507 431 65 443
Fragments arranged by size:
507 443 431 65
Bsp1286I G_dGCh'C
Cuts at: 0 580 643 806 1239 1446
Size: 580 63 163 433 207
Fragments arranged by size:
580 433 207 163 63
BspHI T'CATG_A
Cuts at: 0 1410 1446
Size: 1410 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 173 187 472 1100 1351 1446
Size: 173 14 285 628 251 95
Fragments arranged by size:
628 285 251 173 95 14
BsrBI CCG'CTC
Cuts at: 0 52 1446
Size: 52 1394
BsrDI GCAATG_nn'
Cuts at: 0 1097 1446
Size: 1097 349
BstAPI GCAn_nnn'nTGC
Cuts at: 0 729 1446
Size: 729 717
BstEII G'GTnAC_C
Cuts at: 0 174 1446
Size: 174 1272
BstYI r'GATC_y
Cuts at: 0 845 899 1440 1446
Size: 845 54 541 6
Fragments arranged by size:
845 541 54 6
BtgI C'CryG_G
Cuts at: 0 617 693 1446
Size: 617 76 753
Fragments arranged by size:
753 617 76
BtsI GCAGTG_nn'
Cuts at: 0 659 1446
Size: 659 787
ClaI AT'CG_AT
Cuts at: 0 406 1446
Size: 406 1040
EaeI y'GGCC_r
Cuts at: 0 537 1182 1446
Size: 537 645 264
Fragments arranged by size:
645 537 264
EarI CTCTTCn'nnn_
Cuts at: 0 1049 1304 1446
Size: 1049 255 142
Fragments arranged by size:
1049 255 142
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 140 1446
Size: 19 121 1306
Fragments arranged by size:
1306 121 19
EcoICRI GAG'CTC
Cuts at: 0 804 1446
Size: 804 642
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 581 1409 1446
Size: 581 828 37
Fragments arranged by size:
828 581 37
HaeII r_GCGC'y
Cuts at: 0 891 1446
Size: 891 555
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 932 1446
Size: 393 539 514
Fragments arranged by size:
539 514 393
MslI CAynn'nnrTG
Cuts at: 0 856 1446
Size: 856 590
MspA1I CmG'CkG
Cuts at: 0 58 152 308 636 1446
Size: 58 94 156 328 810
Fragments arranged by size:
810 328 156 94 58
NsiI A_TGCA'T
Cuts at: 0 197 1446
Size: 197 1249
NspI r_CATG'y
Cuts at: 0 714 1171 1446
Size: 714 457 275
Fragments arranged by size:
714 457 275
PasI CC'CwG_GG
Cuts at: 0 114 1446
Size: 114 1332
PciI A'CATG_T
Cuts at: 0 710 1446
Size: 710 736
PfoI T'CCnGG_A
Cuts at: 0 18 1446
Size: 18 1428
PshAI GACnn'nnGTC
Cuts at: 0 221 1446
Size: 221 1225
PspOMI G'GGCC_C
Cuts at: 0 1235 1446
Size: 1235 211
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1446
Size: 1144 32 270
Fragments arranged by size:
1144 270 32
PstI C_TGCA'G
Cuts at: 0 184 313 575 654 725 1362 1446
Size: 184 129 262 79 71 637 84
Fragments arranged by size:
637 262 184 129 84 79 71
PvuII CAG'CTG
Cuts at: 0 636 1446
Size: 636 810
SacI G_AGCT'C
Cuts at: 0 806 1446
Size: 806 640
SbfI CC_TGCA'GG
Cuts at: 0 184 575 1446
Size: 184 391 871
Fragments arranged by size:
871 391 184
ScaI AGT'ACT
Cuts at: 0 1036 1446
Size: 1036 410
SfcI C'TryA_G
Cuts at: 0 180 309 356 473 571 650 721 1358
Size: 180 129 47 117 98 79 71 637
Cuts at: 1358 1446
Size: 88
Fragments arranged by size:
637 180 129 117 98 88 79 71
47
SmlI C'TyrA_G
Cuts at: 0 393 931 1446
Size: 393 538 515
Fragments arranged by size:
538 515 393
SphI G_CATG'C
Cuts at: 0 1171 1446
Size: 1171 275
StyI C'CwwG_G
Cuts at: 0 587 1446
Size: 587 859
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 486 1029 1446
Size: 486 543 417
Fragments arranged by size:
543 486 417
TatI w'GTAC_w
Cuts at: 0 1034 1446
Size: 1034 412
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 823 855 1446
Size: 823 32 591
Fragments arranged by size:
823 591 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1034 1446
Size: 1034 412
XmnI GAAnn'nnTTC
Cuts at: 0 1252 1446
Size: 1252 194
Enzymes that do cut:
AarI AcuI AflIII AlwNI ApaI ApoI BamHI BanII
BfrBI BlpI Bme1580I BmgBI BmrI BpuEI BsaI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BstAPI BstEII BstYI BtgI BtsI ClaI EaeI
EarI EciI EcoICRI Eco57MI HaeII MmeI MslI MspA1I
NsiI NspI PasI PciI PfoI PshAI PspOMI PsrI
PstI PvuII SacI SbfI ScaI SfcI SmlI SphI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BglI BglII BmtI BplI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstZ17I Bsu36I
BtgZI CspCI DraI DraIII DrdI EagI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PvuI
RsrII SacII SalI SanDI SapI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI