(Linear) (Six-base) MAPSORT of: KIBB5833.seq  Check: 8813  from: 1  to: 525



KIBB5833.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AhdI GACnn_n'nnGTC

Cuts at:      0     348     525 
   Size:        348     177
 
AvaI C'yCGr_G

Cuts at:      0      89     353     452     525 
   Size:         89     264      99      73

  Fragments arranged by size: 

                 264      99      89      73
 
BglII A'GATC_T

Cuts at:      0     417     525 
   Size:        417     108
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     208     337     442     525 
   Size:         65     143     129     105      83

  Fragments arranged by size: 

                 143     129     105      83      65
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     196     525 
   Size:        196     329
 
BsaWI w'CCGG_w

Cuts at:      0     225     525 
   Size:        225     300
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     175     205     525 
   Size:        175      30     320

  Fragments arranged by size: 

                 320     175      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      74     304     525 
   Size:         74     230     221

  Fragments arranged by size: 

                 230     221      74
 
BsiEI CG_ry'CG

Cuts at:      0     170     525 
   Size:        170     355
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     313     525 
   Size:        313     212
 
BspEI T'CCGG_A

Cuts at:      0     225     525 
   Size:        225     300
 
BsrBI CCG'CTC

Cuts at:      0     219     270     378     525 
   Size:        219      51     108     147

  Fragments arranged by size: 

                 219     147     108      51
 
BstEII G'GTnAC_C

Cuts at:      0     273     525 
   Size:        273     252
 
BstYI r'GATC_y

Cuts at:      0     299     417     525 
   Size:        299     118     108

  Fragments arranged by size: 

                 299     118     108
 
BtgI C'CryG_G

Cuts at:      0     235     525 
   Size:        235     290
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     327     525 
   Size:        327     198
 
EaeI y'GGCC_r

Cuts at:      0     323     525 
   Size:        323     202
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     109     274     525 
   Size:        109     165     251

  Fragments arranged by size: 

                 251     165     109
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     208     337     442     525 
   Size:         65     143     129     105      83

  Fragments arranged by size: 

                 143     129     105      83      65
 
EcoO109I rG'GnC_Cy

Cuts at:      0     350     525 
   Size:        350     175
 
EcoRV GAT'ATC

Cuts at:      0     449     525 
   Size:        449      76
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      50     525 
   Size:         50     475
 
HaeII r_GCGC'y

Cuts at:      0     508     525 
   Size:        508      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     297     329     334     366     525 
   Size:        297      32       5      32     159

  Fragments arranged by size: 

                 297     159      32      32       5
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     525 
   Size:        173     352
 
MslI CAynn'nnrTG

Cuts at:      0     331     525 
   Size:        331     194
 
MspA1I CmG'CkG

Cuts at:      0     251     525 
   Size:        251     274
 
PasI CC'CwG_GG

Cuts at:      0      67     525 
   Size:         67     458
 
PpuMI rG'GwC_Cy

Cuts at:      0     350     525 
   Size:        350     175
 
SmaI CCC'GGG

Cuts at:      0      91     355     525 
   Size:         91     264     170

  Fragments arranged by size: 

                 264     170      91
 
SmlI C'TyrA_G

Cuts at:      0     452     525 
   Size:        452      73
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     184     525 
   Size:        184     341
 
XbaI T'CTAG_A

Cuts at:      0      37     525 
   Size:         37     488
 
XhoI C'TCGA_G

Cuts at:      0     452     525 
   Size:        452      73
 
XmaI C'CCGG_G

Cuts at:      0      89     353     525 
   Size:         89     264     172

  Fragments arranged by size: 

                 264     172      89
 

 Enzymes that do cut:

     AhdI     AvaI    BglII     BpmI     BsaI    BsaWI    BsaXI    BseRI
    BsiEI    BsmBI    BspEI    BsrBI   BstEII    BstYI     BtgI     DrdI
     EaeI     EciI  Eco57MI EcoO109I    EcoRV     FalI    HaeII    Hin4I
     MmeI     MslI   MspA1I     PasI    PpuMI     SmaI     SmlI    TaqII
     XbaI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaBI    BsaHI    BseYI     BsgI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     EagI     EarI  EcoICRI    EcoNI
    EcoRI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XcmI     XmnI
     ZraI