Restriction map for
pF1KB5832
(Linear) (Six-base) MAPSORT of: KIBB5832.seq Check: 4820 from: 1 to: 1443
KIBB5832.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 214 973 1443
Size: 214 759 470
Fragments arranged by size:
759 470 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 1443
Size: 302 1141
AleI CACnn'nnGTG
Cuts at: 0 169 490 1443
Size: 169 321 953
Fragments arranged by size:
953 321 169
AlwNI CAG_nnn'CTG
Cuts at: 0 565 1443
Size: 565 878
ApaI G_GGCC'C
Cuts at: 0 25 31 461 1175 1443
Size: 25 6 430 714 268
Fragments arranged by size:
714 430 268 25 6
ApaLI G'TGCA_C
Cuts at: 0 198 1443
Size: 198 1245
AvaI C'yCGr_G
Cuts at: 0 24 30 576 967 1443
Size: 24 6 546 391 476
Fragments arranged by size:
546 476 391 24 6
BamHI G'GATC_C
Cuts at: 0 378 1443
Size: 378 1065
BanI G'GyrC_C
Cuts at: 0 86 717 901 915 972 1164 1443
Size: 86 631 184 14 57 192 279
Fragments arranged by size:
631 279 192 184 86 57 14
BanII G_rGCy'C
Cuts at: 0 25 31 461 502 1175 1443
Size: 25 6 430 41 673 268
Fragments arranged by size:
673 430 268 41 25 6
BbeI G_GCGC'C
Cuts at: 0 1168 1443
Size: 1168 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 984 1018 1443
Size: 984 34 425
Fragments arranged by size:
984 425 34
BglI GCCn_nnn'nGGC
Cuts at: 0 35 1443
Size: 35 1408
Bme1580I G_kGCm'C
Cuts at: 0 25 31 202 461 977 1175 1443
Size: 25 6 171 259 516 198 268
Fragments arranged by size:
516 268 259 198 171 25 6
BmgBI CAC'GTC
Cuts at: 0 948 1443
Size: 948 495
BmrI ACTGGGnnnn_n'
Cuts at: 0 1113 1238 1443
Size: 1113 125 205
Fragments arranged by size:
1113 205 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 1443
Size: 90 1353
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 818 1015 1443
Size: 388 430 197 428
Fragments arranged by size:
430 428 388 197
BsaI GGTCTCn'nnnn_
Cuts at: 0 686 1443
Size: 686 757
BsaAI yAC'GTr
Cuts at: 0 171 1443
Size: 171 1272
BsaHI Gr'CG_yC
Cuts at: 0 1165 1443
Size: 1165 278
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 70 100 1443
Size: 70 30 1343
Fragments arranged by size:
1343 70 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 636 1443
Size: 636 807
BseYI C'CCAG_C
Cuts at: 0 105 152 805 860 1195 1251 1360 1443
Size: 105 47 653 55 335 56 109 83
Fragments arranged by size:
653 335 109 105 83 56 55 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 530 689 1443
Size: 514 16 159 754
Fragments arranged by size:
754 514 159 16
BsiHKAI G_wGCw'C
Cuts at: 0 202 628 1443
Size: 202 426 815
Fragments arranged by size:
815 426 202
BsmI GAATG_Cn'
Cuts at: 0 733 1443
Size: 733 710
BsmBI CGTCTCn'nnnn_
Cuts at: 0 557 1443
Size: 557 886
Bsp1286I G_dGCh'C
Cuts at: 0 25 31 202 461 502 628 977 1175
Size: 25 6 171 259 41 126 349 198
Cuts at: 1175 1443
Size: 268
Fragments arranged by size:
349 268 259 198 171 126 41 25
6
BspHI T'CATG_A
Cuts at: 0 1382 1443
Size: 1382 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 214 680 973 1443
Size: 214 466 293 470
Fragments arranged by size:
470 466 293 214
BsrBI CCG'CTC
Cuts at: 0 1029 1443
Size: 1029 414
BstAPI GCAn_nnn'nTGC
Cuts at: 0 206 739 1443
Size: 206 533 704
Fragments arranged by size:
704 533 206
BstEII G'GTnAC_C
Cuts at: 0 162 1443
Size: 162 1281
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 1156 1161 1360 1443
Size: 912 244 5 199 83
Fragments arranged by size:
912 244 199 83 5
BstYI r'GATC_y
Cuts at: 0 378 1364 1443
Size: 378 986 79
Fragments arranged by size:
986 378 79
BtgI C'CryG_G
Cuts at: 0 182 303 1443
Size: 182 121 1140
Fragments arranged by size:
1140 182 121
BtsI GCAGTG_nn'
Cuts at: 0 135 483 804 1197 1223 1443
Size: 135 348 321 393 26 220
Fragments arranged by size:
393 348 321 220 135 26
EaeI y'GGCC_r
Cuts at: 0 1014 1443
Size: 1014 429
EarI CTCTTCn'nnn_
Cuts at: 0 418 816 1006 1308 1443
Size: 418 398 190 302 135
Fragments arranged by size:
418 398 302 190 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 73 79 1443
Size: 73 6 1364
Fragments arranged by size:
1364 73 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 388 818 1015 1443
Size: 302 86 430 197 428
Fragments arranged by size:
430 428 302 197 86
EcoNI CCTnn'n_nnAGG
Cuts at: 0 764 1443
Size: 764 679
EcoO109I rG'GnC_Cy
Cuts at: 0 21 457 964 1020 1171 1232 1443
Size: 21 436 507 56 151 61 211
Fragments arranged by size:
507 436 211 151 61 56 21
EcoRV GAT'ATC
Cuts at: 0 177 1443
Size: 177 1266
HaeII r_GCGC'y
Cuts at: 0 1168 1443
Size: 1168 275
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 419 451 1306 1338 1443
Size: 419 32 855 32 105
Fragments arranged by size:
855 419 105 32 32
KasI G'GCGC_C
Cuts at: 0 1164 1443
Size: 1164 279
MscI TGG'CCA
Cuts at: 0 1016 1443
Size: 1016 427
MslI CAynn'nnrTG
Cuts at: 0 169 490 1443
Size: 169 321 953
Fragments arranged by size:
953 321 169
MspA1I CmG'CkG
Cuts at: 0 47 838 979 1128 1260 1360 1443
Size: 47 791 141 149 132 100 83
Fragments arranged by size:
791 149 141 132 100 83 47
NarI GG'CG_CC
Cuts at: 0 1165 1443
Size: 1165 278
PflMI CCAn_nnn'nTGG
Cuts at: 0 1228 1443
Size: 1228 215
PfoI T'CCnGG_A
Cuts at: 0 404 1443
Size: 404 1039
PpuMI rG'GwC_Cy
Cuts at: 0 964 1020 1443
Size: 964 56 423
Fragments arranged by size:
964 423 56
PspOMI G'GGCC_C
Cuts at: 0 21 27 457 1171 1443
Size: 21 6 430 714 272
Fragments arranged by size:
714 430 272 21 6
PspXI vC'TCGA_Gb
Cuts at: 0 576 1443
Size: 576 867
PstI C_TGCA'G
Cuts at: 0 118 876 984 1443
Size: 118 758 108 459
Fragments arranged by size:
758 459 118 108
PvuII CAG'CTG
Cuts at: 0 838 1360 1443
Size: 838 522 83
Fragments arranged by size:
838 522 83
SanDI GG'GwC_CC
Cuts at: 0 964 1443
Size: 964 479
SapI GCTCTTCn'nnn_
Cuts at: 0 816 1308 1443
Size: 816 492 135
Fragments arranged by size:
816 492 135
SbfI CC_TGCA'GG
Cuts at: 0 876 1443
Size: 876 567
ScaI AGT'ACT
Cuts at: 0 666 1443
Size: 666 777
SfcI C'TryA_G
Cuts at: 0 114 872 980 1443
Size: 114 758 108 463
Fragments arranged by size:
758 463 114 108
SfoI GGC'GCC
Cuts at: 0 1166 1443
Size: 1166 277
SmaI CCC'GGG
Cuts at: 0 26 32 969 1443
Size: 26 6 937 474
Fragments arranged by size:
937 474 26 6
SmlI C'TyrA_G
Cuts at: 0 576 1443
Size: 576 867
SrfI GCCC'GGGC
Cuts at: 0 26 32 1443
Size: 26 6 1411
Fragments arranged by size:
1411 26 6
StyI C'CwwG_G
Cuts at: 0 765 1443
Size: 765 678
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 526 1239 1443
Size: 97 429 713 204
Fragments arranged by size:
713 429 204 97
TatI w'GTAC_w
Cuts at: 0 664 1443
Size: 664 779
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 956 988 1176 1208 1247 1279
Size: 70 32 854 32 188 32 39 32
Cuts at: 1279 1443
Size: 164
Fragments arranged by size:
854 188 164 70 39 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 448 1443
Size: 448 995
XhoI C'TCGA_G
Cuts at: 0 576 1443
Size: 576 867
XmaI C'CCGG_G
Cuts at: 0 24 30 967 1443
Size: 24 6 937 476
Fragments arranged by size:
937 476 24 6
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BcgI BglI Bme1580I BmgBI BmrI
BplI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BstAPI BstEII BstXI BstYI BtgI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I KasI
MscI MslI MspA1I NarI PflMI PfoI PpuMI PspOMI
PspXI PstI PvuII SanDI SapI SbfI ScaI SfcI
SfoI SmaI SmlI SrfI StyI TaqII TatI TstI
Tth111I XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI
BmtI Bpu10I BpuEI BsaBI BsaWI BsiEI BsiWI BspEI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII
SacI SacII SalI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI XbaI XcmI XmnI
ZraI