(Linear) (Six-base) MAPSORT of: KIBB5825.seq  Check: 4544  from: 1  to: 375



KIBB5825.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:17  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     116     375 
   Size:         65      51     259

  Fragments arranged by size: 

                 259      65      51
 
ApoI r'AATT_y

Cuts at:      0      72     375 
   Size:         72     303
 
BclI T'GATC_A

Cuts at:      0     222     375 
   Size:        222     153
 
BfrBI ATG'CAT

Cuts at:      0     217     375 
   Size:        217     158
 
BglI GCCn_nnn'nGGC

Cuts at:      0      22     286     375 
   Size:         22     264      89

  Fragments arranged by size: 

                 264      89      22
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     375 
   Size:        322      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     310     340     375 
   Size:        310      30      35

  Fragments arranged by size: 

                 310      35      30
 
BsmI GAATG_Cn'

Cuts at:      0     219     375 
   Size:        219     156
 
BstYI r'GATC_y

Cuts at:      0     363     375 
   Size:        363      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      19     375 
   Size:         19     356
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     134     375 
   Size:        134     241
 
EaeI y'GGCC_r

Cuts at:      0      14     375 
   Size:         14     361
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      65     116     375 
   Size:         65      51     259

  Fragments arranged by size: 

                 259      65      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     214     246     375 
   Size:        214      32     129

  Fragments arranged by size: 

                 214     129      32
 
HindIII A'AGCT_T

Cuts at:      0     331     375 
   Size:        331      44
 
MspA1I CmG'CkG

Cuts at:      0      94     375 
   Size:         94     281
 
NsiI A_TGCA'T

Cuts at:      0     219     375 
   Size:        219     156
 
NspI r_CATG'y

Cuts at:      0     221     375 
   Size:        221     154
 
PvuII CAG'CTG

Cuts at:      0      94     375 
   Size:         94     281
 
SmlI C'TyrA_G

Cuts at:      0     337     375 
   Size:        337      38
 
TatI w'GTAC_w

Cuts at:      0     269     375 
   Size:        269     106
 

 Enzymes that do cut:

     AcuI     ApoI     BclI    BfrBI     BglI    BpuEI    BsaXI     BsmI
    BstYI    BtgZI     DrdI     EaeI  Eco57MI    Hin4I  HindIII   MspA1I
     NsiI     NspI    PvuII     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI