(Linear) (Six-base) MAPSORT of: KIBB5823.seq  Check: 7469  from: 1  to: 444



KIBB5823.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     205     374     444 
   Size:        205     169      70

  Fragments arranged by size: 

                 205     169      70
 
AleI CACnn'nnGTG

Cuts at:      0     167     444 
   Size:        167     277
 
ApaLI G'TGCA_C

Cuts at:      0     160     444 
   Size:        160     284
 
ApoI r'AATT_y

Cuts at:      0      79     444 
   Size:         79     365
 
BamHI G'GATC_C

Cuts at:      0     348     444 
   Size:        348      96
 
BclI T'GATC_A

Cuts at:      0     140     444 
   Size:        140     304
 
Bme1580I G_kGCm'C

Cuts at:      0      44      62     164     444 
   Size:         44      18     102     280

  Fragments arranged by size: 

                 280     102      44      18
 
BmtI G_CTAG'C

Cuts at:      0      14     444 
   Size:         14     430
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     444 
   Size:         91     353
 
BsaAI yAC'GTr

Cuts at:      0      36     444 
   Size:         36     408
 
BsaBI GATnn'nnATC

Cuts at:      0     335     444 
   Size:        335     109
 
BsiEI CG_ry'CG

Cuts at:      0      88     444 
   Size:         88     356
 
BsiHKAI G_wGCw'C

Cuts at:      0     164     293     444 
   Size:        164     129     151

  Fragments arranged by size: 

                 164     151     129
 
Bsp1286I G_dGCh'C

Cuts at:      0      44      62     164     293     444 
   Size:         44      18     102     129     151

  Fragments arranged by size: 

                 151     129     102      44      18
 
BspHI T'CATG_A

Cuts at:      0     137     444 
   Size:        137     307
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      32     444 
   Size:         32     412
 
BssSI C'ACGA_G

Cuts at:      0      61     444 
   Size:         61     383
 
BstBI TT'CG_AA

Cuts at:      0      77     444 
   Size:         77     367
 
BstYI r'GATC_y

Cuts at:      0     348     444 
   Size:        348      96
 
EaeI y'GGCC_r

Cuts at:      0      85     255     444 
   Size:         85     170     189

  Fragments arranged by size: 

                 189     170      85
 
EagI C'GGCC_G

Cuts at:      0      85     444 
   Size:         85     359
 
EarI CTCTTCn'nnn_

Cuts at:      0     181     444 
   Size:        181     263
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     205     374     444 
   Size:         91     114     169      70

  Fragments arranged by size: 

                 169     114      91      70
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     280     312     444 
   Size:        280      32     132

  Fragments arranged by size: 

                 280     132      32
 
HindIII A'AGCT_T

Cuts at:      0      97     444 
   Size:         97     347
 
MslI CAynn'nnrTG

Cuts at:      0     167     444 
   Size:        167     277
 
NheI G'CTAG_C

Cuts at:      0      10     444 
   Size:         10     434
 
SnaBI TAC'GTA

Cuts at:      0      36     444 
   Size:         36     408
 
StuI AGG'CCT

Cuts at:      0     359     444 
   Size:        359      85
 
XmnI GAAnn'nnTTC

Cuts at:      0     359     444 
   Size:        359      85
 

 Enzymes that do cut:

     AcuI     AleI    ApaLI     ApoI    BamHI     BclI Bme1580I     BmtI
     BpmI    BsaAI    BsaBI    BsiEI  BsiHKAI Bsp1286I    BspHI    BspMI
    BssSI    BstBI    BstYI     EaeI     EagI     EarI  Eco57MI    Hin4I
  HindIII     MslI     NheI    SnaBI     StuI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI     SpeI
     SphI     SrfI     SspI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI