Restriction map for
pF1KB5813
(Linear) (Six-base) MAPSORT of: KIBB5813.seq Check: 6535 from: 1 to: 1422
KIBB5813.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 267 550 610 1112 1272 1422
Size: 267 283 60 502 160 150
Fragments arranged by size:
502 283 267 160 150 60
AatII G_ACGT'C
Cuts at: 0 638 1422
Size: 638 784
AccI GT'mk_AC
Cuts at: 0 98 1422
Size: 98 1324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 366 1422
Size: 366 1056
AflIII A'CryG_T
Cuts at: 0 440 614 995 1422
Size: 440 174 381 427
Fragments arranged by size:
440 427 381 174
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 868 1422
Size: 868 554
AlwNI CAG_nnn'CTG
Cuts at: 0 690 1118 1422
Size: 690 428 304
Fragments arranged by size:
690 428 304
AvaI C'yCGr_G
Cuts at: 0 208 299 418 1422
Size: 208 91 119 1004
Fragments arranged by size:
1004 208 119 91
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 427 460 1422
Size: 427 33 962
Fragments arranged by size:
962 427 33
BanI G'GyrC_C
Cuts at: 0 538 1100 1284 1422
Size: 538 562 184 138
Fragments arranged by size:
562 538 184 138
BanII G_rGCy'C
Cuts at: 0 329 343 498 838 965 1049 1422
Size: 329 14 155 340 127 84 373
Fragments arranged by size:
373 340 329 155 127 84 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 630 1422
Size: 630 792
BbvCI CC'TCA_GC
Cuts at: 0 1229 1422
Size: 1229 193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 145 179 1422
Size: 145 34 1243
Fragments arranged by size:
1243 145 34
BglI GCCn_nnn'nGGC
Cuts at: 0 370 1098 1314 1422
Size: 370 728 216 108
Fragments arranged by size:
728 370 216 108
BglII A'GATC_T
Cuts at: 0 1055 1320 1422
Size: 1055 265 102
Fragments arranged by size:
1055 265 102
Bme1580I G_kGCm'C
Cuts at: 0 541 614 1103 1289 1422
Size: 541 73 489 186 133
Fragments arranged by size:
541 489 186 133 73
BmrI ACTGGGnnnn_n'
Cuts at: 0 1032 1422
Size: 1032 390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 327 498 916 1370 1422
Size: 280 47 171 418 454 52
Fragments arranged by size:
454 418 280 171 52 47
Bpu10I CC'TnA_GC
Cuts at: 0 1229 1422
Size: 1229 193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1422
Size: 658 764
BsaAI yAC'GTr
Cuts at: 0 615 996 1422
Size: 615 381 426
Fragments arranged by size:
615 426 381
BsaHI Gr'CG_yC
Cuts at: 0 635 1422
Size: 635 787
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 471 501 1422
Size: 471 30 921
Fragments arranged by size:
921 471 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 661 1018 1289 1422
Size: 661 357 271 133
Fragments arranged by size:
661 357 271 133
BseYI C'CCAG_C
Cuts at: 0 793 980 1184 1275 1349 1395 1422
Size: 793 187 204 91 74 46 27
Fragments arranged by size:
793 204 187 91 74 46 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 549 1422
Size: 388 161 873
Fragments arranged by size:
873 388 161
BsiEI CG_ry'CG
Cuts at: 0 194 1422
Size: 194 1228
BsiHKAI G_wGCw'C
Cuts at: 0 329 838 893 965 1049 1422
Size: 329 509 55 72 84 373
Fragments arranged by size:
509 373 329 84 72 55
Bsp1286I G_dGCh'C
Cuts at: 0 329 343 498 541 614 838 893 965
Size: 329 14 155 43 73 224 55 72
Cuts at: 965 1049 1103 1289 1422
Size: 84 54 186 133
Fragments arranged by size:
329 224 186 155 133 84 73 72
55 54 43 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 146 267 550 610 1112 1162 1272 1422
Size: 146 121 283 60 502 50 110 150
Fragments arranged by size:
502 283 150 146 121 110 60 50
BsrDI GCAATG_nn'
Cuts at: 0 97 1303 1422
Size: 97 1206 119
Fragments arranged by size:
1206 119 97
BsrFI r'CCGG_y
Cuts at: 0 193 1422
Size: 193 1229
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1118 1422
Size: 1118 304
BstYI r'GATC_y
Cuts at: 0 1055 1320 1422
Size: 1055 265 102
Fragments arranged by size:
1055 265 102
BtgI C'CryG_G
Cuts at: 0 19 658 1028 1212 1312 1422
Size: 19 639 370 184 100 110
Fragments arranged by size:
639 370 184 110 100 19
BtsI GCAGTG_nn'
Cuts at: 0 820 1254 1422
Size: 820 434 168
Fragments arranged by size:
820 434 168
DraIII CAC_nnn'GTG
Cuts at: 0 552 1422
Size: 552 870
EaeI y'GGCC_r
Cuts at: 0 191 251 281 362 371 661 966 1315
Size: 191 60 30 81 9 290 305 349
Cuts at: 1315 1422
Size: 107
Fragments arranged by size:
349 305 290 191 107 81 60 30
9
EagI C'GGCC_G
Cuts at: 0 191 1422
Size: 191 1231
EarI CTCTTCn'nnn_
Cuts at: 0 32 80 334 418 1422
Size: 32 48 254 84 1004
Fragments arranged by size:
1004 254 84 48 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 212 1422
Size: 212 1210
EcoICRI GAG'CTC
Cuts at: 0 327 836 963 1047 1422
Size: 327 509 127 84 375
Fragments arranged by size:
509 375 327 127 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 327 366 498 916 1370 1422
Size: 280 47 39 132 418 454 52
Fragments arranged by size:
454 418 280 132 52 47 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 477 883 1422
Size: 477 406 539
Fragments arranged by size:
539 477 406
EcoO109I rG'GnC_Cy
Cuts at: 0 135 214 459 1399 1407 1422
Size: 135 79 245 940 8 15
Fragments arranged by size:
940 245 135 79 15 8
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 930 1422
Size: 930 492
FspI TGC'GCA
Cuts at: 0 583 1422
Size: 583 839
HaeII r_GCGC'y
Cuts at: 0 81 1422
Size: 81 1341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 1422
Size: 511 32 879
Fragments arranged by size:
879 511 32
HincII GTy'rAC
Cuts at: 0 99 699 1422
Size: 99 600 723
Fragments arranged by size:
723 600 99
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1250 1422
Size: 1250 172
MscI TGG'CCA
Cuts at: 0 253 364 373 663 1422
Size: 253 111 9 290 759
Fragments arranged by size:
759 290 253 111 9
MslI CAynn'nnrTG
Cuts at: 0 994 1422
Size: 994 428
MspA1I CmG'CkG
Cuts at: 0 159 360 608 797 1011 1115 1169 1390
Size: 159 201 248 189 214 104 54 221
Cuts at: 1390 1422
Size: 32
Fragments arranged by size:
248 221 214 201 189 159 104 54
32
NaeI GCC'GGC
Cuts at: 0 195 1422
Size: 195 1227
NcoI C'CATG_G
Cuts at: 0 658 1028 1422
Size: 658 370 394
Fragments arranged by size:
658 394 370
NgoMIV G'CCGG_C
Cuts at: 0 193 1422
Size: 193 1229
NspI r_CATG'y
Cuts at: 0 393 444 1422
Size: 393 51 978
Fragments arranged by size:
978 393 51
PasI CC'CwG_GG
Cuts at: 0 51 512 1422
Size: 51 461 910
Fragments arranged by size:
910 461 51
PciI A'CATG_T
Cuts at: 0 440 1422
Size: 440 982
PflMI CCAn_nnn'nTGG
Cuts at: 0 552 1422
Size: 552 870
PfoI T'CCnGG_A
Cuts at: 0 893 1422
Size: 893 529
PmlI CAC'GTG
Cuts at: 0 615 996 1422
Size: 615 381 426
Fragments arranged by size:
615 426 381
PpuMI rG'GwC_Cy
Cuts at: 0 135 214 1399 1407 1422
Size: 135 79 1185 8 15
Fragments arranged by size:
1185 135 79 15 8
PstI C_TGCA'G
Cuts at: 0 77 607 755 763 857 1016 1114 1422
Size: 77 530 148 8 94 159 98 308
Fragments arranged by size:
530 308 159 148 98 94 77 8
PvuII CAG'CTG
Cuts at: 0 159 360 797 1011 1115 1390 1422
Size: 159 201 437 214 104 275 32
Fragments arranged by size:
437 275 214 201 159 104 32
SacI G_AGCT'C
Cuts at: 0 329 838 965 1049 1422
Size: 329 509 127 84 373
Fragments arranged by size:
509 373 329 127 84
SalI G'TCGA_C
Cuts at: 0 97 1422
Size: 97 1325
SanDI GG'GwC_CC
Cuts at: 0 214 1399 1422
Size: 214 1185 23
Fragments arranged by size:
1185 214 23
SapI GCTCTTCn'nnn_
Cuts at: 0 80 334 1422
Size: 80 254 1088
Fragments arranged by size:
1088 254 80
SbfI CC_TGCA'GG
Cuts at: 0 763 1422
Size: 763 659
ScaI AGT'ACT
Cuts at: 0 430 1422
Size: 430 992
SfcI C'TryA_G
Cuts at: 0 73 603 751 759 853 1012 1110 1422
Size: 73 530 148 8 94 159 98 312
Fragments arranged by size:
530 312 159 148 98 94 73 8
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 370 1422
Size: 370 1052
SmaI CCC'GGG
Cuts at: 0 210 301 420 1422
Size: 210 91 119 1002
Fragments arranged by size:
1002 210 119 91
SmlI C'TyrA_G
Cuts at: 0 673 1422
Size: 673 749
StuI AGG'CCT
Cuts at: 0 757 1422
Size: 757 665
StyI C'CwwG_G
Cuts at: 0 455 658 1028 1422
Size: 455 203 370 394
Fragments arranged by size:
455 394 370 203
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 719 1422
Size: 719 703
TatI w'GTAC_w
Cuts at: 0 428 799 1422
Size: 428 371 623
Fragments arranged by size:
623 428 371
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1233 1265 1422
Size: 1233 32 157
Fragments arranged by size:
1233 157 32
XmaI C'CCGG_G
Cuts at: 0 208 299 418 1422
Size: 208 91 119 1004
Fragments arranged by size:
1004 208 119 91
ZraI GAC'GTC
Cuts at: 0 636 1422
Size: 636 786
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AlfI AlwNI AvaI
BaeI BanI BanII BbsI BbvCI BcgI BglI BglII
Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BstAPI BstYI BtgI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I HincII MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI
PpuMI PstI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SfiI SmaI SmlI StuI StyI TaqII
TatI TstI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AloI
ApaI ApaLI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BciVI BclI BfrBI BlpI BmgBI BmtI BplI
BsaI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV
FseI FspAI HindIII HpaI KasI KpnI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PmeI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SexAI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI