Restriction map for
pF1KB5807
(Linear) (Six-base) MAPSORT of: KIBB5807.seq Check: 3567 from: 1 to: 1416
KIBB5807.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 16 1416
Size: 16 1400
Acc65I G'GTAC_C
Cuts at: 0 449 1416
Size: 449 967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 201 745 766 1416
Size: 201 544 21 650
Fragments arranged by size:
650 544 201 21
AhdI GACnn_n'nnGTC
Cuts at: 0 1088 1416
Size: 1088 328
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 482 1416
Size: 482 934
AlwNI CAG_nnn'CTG
Cuts at: 0 1020 1416
Size: 1020 396
ApaLI G'TGCA_C
Cuts at: 0 1363 1416
Size: 1363 53
BanI G'GyrC_C
Cuts at: 0 449 1416
Size: 449 967
BanII G_rGCy'C
Cuts at: 0 49 1001 1416
Size: 49 952 415
Fragments arranged by size:
952 415 49
BbsI GAAGACnn'nnnn_
Cuts at: 0 1219 1416
Size: 1219 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1384 1416
Size: 1384 32
BglI GCCn_nnn'nGGC
Cuts at: 0 583 1416
Size: 583 833
BglII A'GATC_T
Cuts at: 0 989 1416
Size: 989 427
BlpI GC'TnA_GC
Cuts at: 0 1045 1416
Size: 1045 371
Bme1580I G_kGCm'C
Cuts at: 0 1367 1416
Size: 1367 49
BmgBI CAC'GTC
Cuts at: 0 1209 1416
Size: 1209 207
BmrI ACTGGGnnnn_n'
Cuts at: 0 1281 1416
Size: 1281 135
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 66 427 724 1042 1084 1096 1243
Size: 15 51 361 297 318 42 12 147
Cuts at: 1243 1276 1314 1416
Size: 33 38 102
Fragments arranged by size:
361 318 297 147 102 51 42 38
33 15 12
Bpu10I CC'TnA_GC
Cuts at: 0 838 1416
Size: 838 578
BsaI GGTCTCn'nnnn_
Cuts at: 0 1075 1416
Size: 1075 341
BsaHI Gr'CG_yC
Cuts at: 0 1268 1416
Size: 1268 148
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1264 1294 1416
Size: 1264 30 122
Fragments arranged by size:
1264 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 84 162 424 745 775 778 946
Size: 18 66 78 262 321 30 3 168
Cuts at: 946 1093 1156 1269 1272 1284 1317 1416
Size: 147 63 113 3 12 33 99
Fragments arranged by size:
321 262 168 147 113 99 78 66
63 33 30 18 12 3 3
BseYI C'CCAG_C
Cuts at: 0 127 1040 1154 1416
Size: 127 913 114 262
Fragments arranged by size:
913 262 127 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 991 1416
Size: 806 185 425
Fragments arranged by size:
806 425 185
BsiEI CG_ry'CG
Cuts at: 0 372 1416
Size: 372 1044
BsiHKAI G_wGCw'C
Cuts at: 0 405 1001 1367 1416
Size: 405 596 366 49
Fragments arranged by size:
596 405 366 49
BsmI GAATG_Cn'
Cuts at: 0 777 845 1416
Size: 777 68 571
Fragments arranged by size:
777 571 68
Bsp1286I G_dGCh'C
Cuts at: 0 49 405 1001 1367 1416
Size: 49 356 596 366 49
Fragments arranged by size:
596 366 356 49 49
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 635 1386 1416
Size: 16 619 751 30
Fragments arranged by size:
751 619 30 16
BsrBI CCG'CTC
Cuts at: 0 977 1416
Size: 977 439
BssSI C'ACGA_G
Cuts at: 0 757 1390 1416
Size: 757 633 26
Fragments arranged by size:
757 633 26
BstEII G'GTnAC_C
Cuts at: 0 348 1416
Size: 348 1068
BstXI CCAn_nnnn'nTGG
Cuts at: 0 793 1356 1416
Size: 793 563 60
Fragments arranged by size:
793 563 60
BstYI r'GATC_y
Cuts at: 0 437 989 1115 1196 1416
Size: 437 552 126 81 220
Fragments arranged by size:
552 437 220 126 81
Bsu36I CC'TnA_GG
Cuts at: 0 514 1416
Size: 514 902
BtgI C'CryG_G
Cuts at: 0 587 1416
Size: 587 829
BtsI GCAGTG_nn'
Cuts at: 0 345 1416
Size: 345 1071
EaeI y'GGCC_r
Cuts at: 0 104 584 689 950 1416
Size: 104 480 105 261 466
Fragments arranged by size:
480 466 261 105 104
EcoICRI GAG'CTC
Cuts at: 0 999 1416
Size: 999 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 66 201 427 724 745 766 1042
Size: 15 51 135 226 297 21 21 276
Cuts at: 1042 1084 1096 1243 1276 1314 1416
Size: 42 12 147 33 38 102
Fragments arranged by size:
297 276 226 147 135 102 51 42
38 33 21 21 15 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 727 1255 1416
Size: 391 336 528 161
Fragments arranged by size:
528 391 336 161
EcoO109I rG'GnC_Cy
Cuts at: 0 113 510 1398 1416
Size: 113 397 888 18
Fragments arranged by size:
888 397 113 18
FspI TGC'GCA
Cuts at: 0 631 664 1416
Size: 631 33 752
Fragments arranged by size:
752 631 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1021 1053 1074 1106 1309 1341 1416
Size: 1021 32 21 32 203 32 75
Fragments arranged by size:
1021 203 75 32 32 32 21
KpnI G_GTAC'C
Cuts at: 0 453 1416
Size: 453 963
MscI TGG'CCA
Cuts at: 0 691 952 1416
Size: 691 261 464
Fragments arranged by size:
691 464 261
MslI CAynn'nnrTG
Cuts at: 0 637 1360 1416
Size: 637 723 56
Fragments arranged by size:
723 637 56
MspA1I CmG'CkG
Cuts at: 0 462 589 1107 1149 1163 1416
Size: 462 127 518 42 14 253
Fragments arranged by size:
518 462 253 127 42 14
NspI r_CATG'y
Cuts at: 0 1104 1416
Size: 1104 312
PfoI T'CCnGG_A
Cuts at: 0 1118 1416
Size: 1118 298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 898 930 1416
Size: 898 32 486
Fragments arranged by size:
898 486 32
PpuMI rG'GwC_Cy
Cuts at: 0 113 510 1398 1416
Size: 113 397 888 18
Fragments arranged by size:
888 397 113 18
PshAI GACnn'nnGTC
Cuts at: 0 485 1416
Size: 485 931
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 1416
Size: 1174 32 210
Fragments arranged by size:
1174 210 32
PstI C_TGCA'G
Cuts at: 0 13 566 1037 1416
Size: 13 553 471 379
Fragments arranged by size:
553 471 379 13
PvuII CAG'CTG
Cuts at: 0 1107 1149 1416
Size: 1107 42 267
Fragments arranged by size:
1107 267 42
SacI G_AGCT'C
Cuts at: 0 1001 1416
Size: 1001 415
SacII CC_GC'GG
Cuts at: 0 590 1416
Size: 590 826
SfcI C'TryA_G
Cuts at: 0 9 483 562 1033 1416
Size: 9 474 79 471 383
Fragments arranged by size:
474 471 383 79 9
SphI G_CATG'C
Cuts at: 0 1104 1416
Size: 1104 312
StyI C'CwwG_G
Cuts at: 0 273 1349 1416
Size: 273 1076 67
Fragments arranged by size:
1076 273 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 277 781 1402 1416
Size: 277 504 621 14
Fragments arranged by size:
621 504 277 14
TatI w'GTAC_w
Cuts at: 0 1190 1416
Size: 1190 226
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1264 1296 1416
Size: 1264 32 120
Fragments arranged by size:
1264 120 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 423 699 963 1017 1126 1294 1416
Size: 423 276 264 54 109 168 122
Fragments arranged by size:
423 276 264 168 122 109 54
Enzymes that do cut:
AarI Acc65I AcuI AhdI AlfI AlwNI ApaLI BanI
BanII BbsI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BssSI
BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EcoICRI
Eco57MI EcoNI EcoO109I FspI Hin4I KpnI MscI MslI
MspA1I NspI PfoI PpiI PpuMI PshAI PsrI PstI
PvuII SacI SacII SfcI SphI StyI TaqII TatI
TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AleI
AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BmtI
BplI BpuEI BsaAI BsaBI BsaWI BsiWI BsmBI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoRI EcoRV FalI FseI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PsiI PspOMI PspXI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI
XmnI ZraI