Restriction map for
pF1KB5801
(Linear) (Six-base) MAPSORT of: KIBB5801.seq Check: 9202 from: 1 to: 1113
KIBB5801.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AflIII A'CryG_T
Cuts at: 0 614 1113
Size: 614 499
AlwNI CAG_nnn'CTG
Cuts at: 0 992 1113
Size: 992 121
ApoI r'AATT_y
Cuts at: 0 55 288 336 1096 1113
Size: 55 233 48 760 17
Fragments arranged by size:
760 233 55 48 17
AvaI C'yCGr_G
Cuts at: 0 492 562 770 1113
Size: 492 70 208 343
Fragments arranged by size:
492 343 208 70
BamHI G'GATC_C
Cuts at: 0 557 942 1113
Size: 557 385 171
Fragments arranged by size:
557 385 171
BanII G_rGCy'C
Cuts at: 0 531 802 1113
Size: 531 271 311
Fragments arranged by size:
531 311 271
BbsI GAAGACnn'nnnn_
Cuts at: 0 441 640 832 1113
Size: 441 199 192 281
Fragments arranged by size:
441 281 199 192
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 142 176 697 731 959 993 1113
Size: 142 34 521 34 228 34 120
Fragments arranged by size:
521 228 142 120 34 34 34
BglII A'GATC_T
Cuts at: 0 27 1113
Size: 27 1086
BmrI ACTGGGnnnn_n'
Cuts at: 0 715 805 1031 1113
Size: 715 90 226 82
Fragments arranged by size:
715 226 90 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 1113
Size: 669 444
BsaI GGTCTCn'nnnn_
Cuts at: 0 465 1113
Size: 465 648
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 428 458 516 546 1113
Size: 428 30 58 30 567
Fragments arranged by size:
567 428 58 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 607 610 754 757 1113
Size: 604 3 3 144 3 356
Fragments arranged by size:
604 356 144 3 3 3
BseYI C'CCAG_C
Cuts at: 0 866 1113
Size: 866 247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 1113
Size: 210 903
BsiHKAI G_wGCw'C
Cuts at: 0 531 1113
Size: 531 582
Bsp1286I G_dGCh'C
Cuts at: 0 531 802 1113
Size: 531 271 311
Fragments arranged by size:
531 311 271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 674 1113
Size: 674 439
BsrDI GCAATG_nn'
Cuts at: 0 386 954 1113
Size: 386 568 159
Fragments arranged by size:
568 386 159
BsrFI r'CCGG_y
Cuts at: 0 995 1113
Size: 995 118
BssSI C'ACGA_G
Cuts at: 0 144 1113
Size: 144 969
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 385 992 1113
Size: 188 197 607 121
Fragments arranged by size:
607 197 188 121
BstBI TT'CG_AA
Cuts at: 0 903 1113
Size: 903 210
BstXI CCAn_nnnn'nTGG
Cuts at: 0 866 889 1113
Size: 866 23 224
Fragments arranged by size:
866 224 23
BstYI r'GATC_y
Cuts at: 0 27 557 942 1113
Size: 27 530 385 171
Fragments arranged by size:
530 385 171 27
BtgI C'CryG_G
Cuts at: 0 80 429 584 638 1113
Size: 80 349 155 54 475
Fragments arranged by size:
475 349 155 80 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 232 1113
Size: 232 881
DraI TTT'AAA
Cuts at: 0 359 1113
Size: 359 754
EarI CTCTTCn'nnn_
Cuts at: 0 596 914 1113
Size: 596 318 199
Fragments arranged by size:
596 318 199
EcoICRI GAG'CTC
Cuts at: 0 529 1113
Size: 529 584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 669 1113
Size: 669 444
EcoNI CCTnn'n_nnAGG
Cuts at: 0 968 1090 1113
Size: 968 122 23
Fragments arranged by size:
968 122 23
EcoO109I rG'GnC_Cy
Cuts at: 0 176 551 566 1026 1113
Size: 176 375 15 460 87
Fragments arranged by size:
460 375 176 87 15
EcoRI G'AATT_C
Cuts at: 0 288 1113
Size: 288 825
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 400 1113
Size: 63 337 713
Fragments arranged by size:
713 337 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 428 448 460 480 516 548
Size: 292 32 104 20 12 20 36 32
Cuts at: 548 1113
Size: 565
Fragments arranged by size:
565 292 104 36 32 32 20 20
12
HindIII A'AGCT_T
Cuts at: 0 411 1113
Size: 411 702
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 479 728 1113
Size: 329 150 249 385
Fragments arranged by size:
385 329 249 150
MslI CAynn'nnrTG
Cuts at: 0 619 1113
Size: 619 494
NaeI GCC'GGC
Cuts at: 0 997 1113
Size: 997 116
NcoI C'CATG_G
Cuts at: 0 80 584 1113
Size: 80 504 529
Fragments arranged by size:
529 504 80
NgoMIV G'CCGG_C
Cuts at: 0 995 1113
Size: 995 118
NspI r_CATG'y
Cuts at: 0 618 1072 1113
Size: 618 454 41
Fragments arranged by size:
618 454 41
PciI A'CATG_T
Cuts at: 0 614 1113
Size: 614 499
PfoI T'CCnGG_A
Cuts at: 0 505 553 1113
Size: 505 48 560
Fragments arranged by size:
560 505 48
PpuMI rG'GwC_Cy
Cuts at: 0 176 551 566 1026 1113
Size: 176 375 15 460 87
Fragments arranged by size:
460 375 176 87 15
PshAI GACnn'nnGTC
Cuts at: 0 1024 1113
Size: 1024 89
PstI C_TGCA'G
Cuts at: 0 155 763 853 988 1113
Size: 155 608 90 135 125
Fragments arranged by size:
608 155 135 125 90
SacI G_AGCT'C
Cuts at: 0 531 1113
Size: 531 582
SanDI GG'GwC_CC
Cuts at: 0 566 1113
Size: 566 547
SexAI A'CCwGG_T
Cuts at: 0 541 1113
Size: 541 572
SfcI C'TryA_G
Cuts at: 0 151 171 759 849 984 1113
Size: 151 20 588 90 135 129
Fragments arranged by size:
588 151 135 129 90 20
SmaI CCC'GGG
Cuts at: 0 494 1113
Size: 494 619
SmlI C'TyrA_G
Cuts at: 0 770 1113
Size: 770 343
SphI G_CATG'C
Cuts at: 0 1072 1113
Size: 1072 41
StyI C'CwwG_G
Cuts at: 0 80 584 1113
Size: 80 504 529
Fragments arranged by size:
529 504 80
TatI w'GTAC_w
Cuts at: 0 611 1113
Size: 611 502
XhoI C'TCGA_G
Cuts at: 0 770 1113
Size: 770 343
XmaI C'CCGG_G
Cuts at: 0 492 1113
Size: 492 621
Enzymes that do cut:
AflIII AlwNI ApoI AvaI BamHI BanII BbsI BcgI
BglII BmrI BpmI BsaI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI BstBI
BstXI BstYI BtgI BtgZI DraI EarI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI Hin4I HindIII MmeI MslI
NaeI NcoI NgoMIV NspI PciI PfoI PpuMI PshAI
PstI SacI SanDI SexAI SfcI SmaI SmlI SphI
StyI TatI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbvCI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstEII
BstZ17I Bsu36I BtsI ClaI CspCI DraIII DrdI EaeI
EagI EciI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI MspA1I NarI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SrfI SspI StuI
SwaI TaqII TstI Tth111I XbaI XcmI XmnI ZraI