Restriction map for
pF1KB5766
(Linear) (Six-base) MAPSORT of: KIBB5766.seq Check: 4724 from: 1 to: 2241
KIBB5766.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 412 522 2241
Size: 412 110 1719
Fragments arranged by size:
1719 412 110
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 395 584 1664 2008 2125 2161 2241
Size: 89 306 189 1080 344 117 36 80
Fragments arranged by size:
1080 344 306 189 117 89 80 36
AleI CACnn'nnGTG
Cuts at: 0 1337 2227 2241
Size: 1337 890 14
Fragments arranged by size:
1337 890 14
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 698 2241
Size: 698 1543
AlwNI CAG_nnn'CTG
Cuts at: 0 448 1503 1642 1962 1986 2241
Size: 448 1055 139 320 24 255
Fragments arranged by size:
1055 448 320 255 139 24
ApoI r'AATT_y
Cuts at: 0 1489 1786 2241
Size: 1489 297 455
Fragments arranged by size:
1489 455 297
AvaI C'yCGr_G
Cuts at: 0 1061 2241
Size: 1061 1180
BanII G_rGCy'C
Cuts at: 0 1866 2006 2241
Size: 1866 140 235
Fragments arranged by size:
1866 235 140
BbsI GAAGACnn'nnnn_
Cuts at: 0 1714 2241
Size: 1714 527
BbvCI CC'TCA_GC
Cuts at: 0 1979 2241
Size: 1979 262
BclI T'GATC_A
Cuts at: 0 480 1203 1440 2241
Size: 480 723 237 801
Fragments arranged by size:
801 723 480 237
BmtI G_CTAG'C
Cuts at: 0 2083 2241
Size: 2083 158
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1533 2241
Size: 1533 708
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 161 389 1082 1802 2095 2241
Size: 161 228 693 720 293 146
Fragments arranged by size:
720 693 293 228 161 146
Bpu10I CC'TnA_GC
Cuts at: 0 1022 1979 2241
Size: 1022 957 262
Fragments arranged by size:
1022 957 262
BsaI GGTCTCn'nnnn_
Cuts at: 0 1846 2241
Size: 1846 395
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1930 1960 2241
Size: 1930 30 281
Fragments arranged by size:
1930 281 30
BseYI C'CCAG_C
Cuts at: 0 441 919 1409 1740 2241
Size: 441 478 490 331 501
Fragments arranged by size:
501 490 478 441 331
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 500 2241
Size: 500 1741
BsiHKAI G_wGCw'C
Cuts at: 0 2006 2241
Size: 2006 235
BsmI GAATG_Cn'
Cuts at: 0 699 2241
Size: 699 1542
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1549 2241
Size: 1549 692
Bsp1286I G_dGCh'C
Cuts at: 0 1866 2006 2241
Size: 1866 140 235
Fragments arranged by size:
1866 235 140
BspHI T'CATG_A
Cuts at: 0 1041 2241
Size: 1041 1200
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 412 522 2241
Size: 412 110 1719
Fragments arranged by size:
1719 412 110
BsrDI GCAATG_nn'
Cuts at: 0 76 292 1178 1910 2241
Size: 76 216 886 732 331
Fragments arranged by size:
886 732 331 216 76
BsrGI T'GTAC_A
Cuts at: 0 1841 2241
Size: 1841 400
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2124 2241
Size: 2124 117
Bsu36I CC'TnA_GG
Cuts at: 0 461 1459 2012 2241
Size: 461 998 553 229
Fragments arranged by size:
998 553 461 229
BtgI C'CryG_G
Cuts at: 0 743 1388 1833 2241
Size: 743 645 445 408
Fragments arranged by size:
743 645 445 408
ClaI AT'CG_AT
Cuts at: 0 307 2241
Size: 307 1934
DraI TTT'AAA
Cuts at: 0 473 1074 2031 2241
Size: 473 601 957 210
Fragments arranged by size:
957 601 473 210
EarI CTCTTCn'nnn_
Cuts at: 0 259 860 1640 1844 2241
Size: 259 601 780 204 397
Fragments arranged by size:
780 601 397 259 204
EcoICRI GAG'CTC
Cuts at: 0 2004 2241
Size: 2004 237
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 161 389 395 584 1082 1664 1802
Size: 89 72 228 6 189 498 582 138
Cuts at: 1802 2008 2095 2125 2161 2241
Size: 206 87 30 36 80
Fragments arranged by size:
582 498 228 206 189 138 89 87
80 72 36 30 6
EcoRI G'AATT_C
Cuts at: 0 1786 2241
Size: 1786 455
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1873 2241
Size: 1873 368
HaeII r_GCGC'y
Cuts at: 0 2207 2241
Size: 2207 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 760 792 960 992 1138 1156
Size: 418 32 310 32 168 32 146 18
Cuts at: 1156 1170 1188 2241
Size: 14 18 1053
Fragments arranged by size:
1053 418 310 168 146 32 32 32
18 18 14
HincII GTy'rAC
Cuts at: 0 122 1563 2241
Size: 122 1441 678
Fragments arranged by size:
1441 678 122
HindIII A'AGCT_T
Cuts at: 0 1796 2241
Size: 1796 445
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 749 2241
Size: 749 1492
MslI CAynn'nnrTG
Cuts at: 0 1337 2227 2241
Size: 1337 890 14
Fragments arranged by size:
1337 890 14
MspA1I CmG'CkG
Cuts at: 0 1209 1429 1740 2103 2241
Size: 1209 220 311 363 138
Fragments arranged by size:
1209 363 311 220 138
NcoI C'CATG_G
Cuts at: 0 743 1388 2241
Size: 743 645 853
Fragments arranged by size:
853 743 645
NdeI CA'TA_TG
Cuts at: 0 2043 2196 2241
Size: 2043 153 45
Fragments arranged by size:
2043 153 45
NheI G'CTAG_C
Cuts at: 0 2079 2241
Size: 2079 162
PfoI T'CCnGG_A
Cuts at: 0 212 2241
Size: 212 2029
PsiI TTA'TAA
Cuts at: 0 284 2241
Size: 284 1957
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1514 1546 2241
Size: 1514 32 695
Fragments arranged by size:
1514 695 32
PstI C_TGCA'G
Cuts at: 0 279 453 677 948 1311 1722 2241
Size: 279 174 224 271 363 411 519
Fragments arranged by size:
519 411 363 279 271 224 174
PvuII CAG'CTG
Cuts at: 0 1209 1429 2103 2241
Size: 1209 220 674 138
Fragments arranged by size:
1209 674 220 138
SacI G_AGCT'C
Cuts at: 0 2006 2241
Size: 2006 235
ScaI AGT'ACT
Cuts at: 0 1939 2241
Size: 1939 302
SfcI C'TryA_G
Cuts at: 0 275 449 673 944 1307 1718 1954 2241
Size: 275 174 224 271 363 411 236 287
Fragments arranged by size:
411 363 287 275 271 236 224 174
StyI C'CwwG_G
Cuts at: 0 743 1265 1388 2241
Size: 743 522 123 853
Fragments arranged by size:
853 743 522 123
SwaI ATTT'AAAT
Cuts at: 0 2031 2241
Size: 2031 210
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 426 1103 1807 2241
Size: 426 677 704 434
Fragments arranged by size:
704 677 434 426
TatI w'GTAC_w
Cuts at: 0 1697 1841 1937 2241
Size: 1697 144 96 304
Fragments arranged by size:
1697 304 144 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1718 1750 2241
Size: 1718 32 491
Fragments arranged by size:
1718 491 32
XmnI GAAnn'nnTTC
Cuts at: 0 1106 2241
Size: 1106 1135
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApoI AvaI BanII
BbsI BbvCI BclI BmtI BplI BpmI Bpu10I BsaI
BsaXI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrDI BsrGI BstXI Bsu36I BtgI ClaI DraI
EarI EcoICRI Eco57MI EcoRI FalI HaeII Hin4I HincII
HindIII MmeI MslI MspA1I NcoI NdeI NheI PfoI
PsiI PsrI PstI PvuII SacI ScaI SfcI StyI
SwaI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BcgI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BpuEI BsaAI BsaBI
BsaHI BsaWI BseRI BsiEI BsiWI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EciI EcoNI
EcoO109I EcoRV FseI FspI FspAI HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NgoMIV NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI Tth111I XbaI XcmI XhoI XmaI ZraI